Influence of Diabetes on Morphometric Index of Ovarian Follicles in Streptozocin-Induced Rats

The study aimed to determine the effect of diabetes on follicle development by measuring the diameters of rats' ovarian follicles. The structure and function of many organs change by being affected by diabetes. The ovary is an essential organ of the reproductive system affected by diabetes. The size of the ovarian follicles and corpus luteum can also be affected by diabetes. For this reason, body mass, blood glucose level, and rat ovarian follicles, and corpus luteum diameters were measured in this study. Ten healthy female rats were kept as the control group. In the other rat group, experimental diabetes was induced with streptozotocin (STZ)(60 mg/kg). The rats in both groups were killed after 30 days and their ovaries removed. 5-6 µm sections were made using paraffin embedding techniques and stained with hematoxylin-eosin. On the fifth day of STZ administration to rats, the mass loss of rats was 10%, and the diabetogenic index was 330%. Compared with the control group, diameters of diabetic rats significantly decreased the diameter of the primordial, primary and Graafian follicles, and corpus luteum. The decrease in the diameter of secondary follicles of diabetic rats was not significant. The percentage shrinkage index was the highest in the corpus luteum with 37%. As a result, it can be said that diabetes influences the size of the ovarian follicles and especially the corpus luteum, thereby negatively affecting the ability to improve oocyte quality. Diabetes-related follicle diameter may shrink and cause infertility. It may be essential to measure the diameters of the follicles in vitro fertilization studies in patients with diabetes

Stimuli Responsive Hydrogels: NIPAM/AAm/Carboxylic Acid Polymers

Stimuli-responsive hydrogels (SRH) were prepared by using monomers (i.e. N-isopropyl acrylamide; NIPAM and acrylamide; AAm), co-monomers (i.e. methacrylic acid; MPA or mesaconic acid; MFA) and a crosslinker (N, N’-methylene bisacrylamide; N-Bis). SRH have been prepared by thermal free radical polymerization reaction in aqueous solution. Spectroscopic and thermal analyses such as Fourier Transform Infrared Spectroscopy, thermogravimetric analysis and differential scanning calorimetry analysis were performed for SRH characterization. The equilibrium swelling studies by gravimetrically were carried out in different solvents, at the solutions, temperature, pH, and ionic strengths to determine their effect on swelling characteristic of the hydrogels. In addition, cycles equilibrium swelling studies were made with the solutions at different temperatures and at different pH. NIPAM/AAm hydrogel exhibits a lover critical solution temperature (LCST) at 28 oC, whereas NIPAM/AAm-MPA and NIPAM/AAm-MFA hydrogels exhibit a LCST at 31 C and 35 oC, respectively, and the LCST of NIPAM/AAm-MFA hydrogel is close to the body temperature

Synthesis of New Molecular Imprinted Polymer for Highly Recognition of Cholic Acid

In this study, molecular imprinted polymers for highly selective recognition of cholic acid, which is a bile acid, were prepared. Acrylamide, methacrylic acid, methacrylamide were chosen as monomer for the production of molecularly imprinted polymers. Functional monomers were polymerized with various crosslinkers; ethylene glycol dimethacrylate (EGDMA), 1,4-butanediol diacrylate (BUT), trimethylpropane triacrylate (TMT) with target molecule (MIP-EGDMA, MIP-BUT and MIP TMT) and various MIPs were prepared. The cholic acid was removed from MIP with a suitable method. NIP polymers were synthesized without cholic acid (NIPEGDMA, NIP-BUT and NIP-TMT). For the characterization of synthesized polymers FTIR, DSC, TGA, SEM analyses were used. The parameters that affect the adsorption of target species on polymers such as temperature, pH, and concentration were evaluated. The selectivity and reusability studies were also investigated

Adsorption of Bovine Serum Albumin Onto Radiation-Crosslinked Poly(Acrylamide/Acrylic Acid)

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