Tetra­aqua­bis(orotato-κO)cobalt(II) dihydrate

In the title CoII complex, [Co(C5H3N2O4)2(H2O)4]·2H2O, the CoII ion is located on an inversion center and is coordinated by two orotate (2,6-dioxo-1,2,3,6-tetrahydropyrimidine-4-carboxylate) anions and four water mol­ecules in a slightly distorted octa­hedral geometry. The dihedral angle between the carboxyl­ate group and the attached orotate ring is 1.2 (3)°. In the crystal structure, inter­molecular O—H⋯O, N—H⋯O and C—H⋯O hydrogen bonds link the mol­ecules into a three-dimensional network. π–π contacts between the orotate rings [centroid–centroid distances = 3.439 (2) and 3.438 (2) Å] further stabilize the structure

Degradation of some pesticides widely using in Bursa, by soil bacteria

Bu çalışmada, Bursa ve çevresinde yaygın olarak kullanılan pestisitlerden Chloridazon, Chlorpyrifos -ethyl ve mukayese materyali olarak da pp'- DDT'nin, Pseııdomonas aeruginosa DSM 50071, Bacillus megaterium DSM 32 ve Bacillus subtilis IMG 22 bakterileri tarafından parçalanma durumları araştırılmıştır. Pestisitlerin bakteriler tarafından parçalanması, optimum sıcaklık istekleri göz önüne alınarak Mineral Salts Yeast Extract (MSYE) ve Minimal Glucose Medium (MGM) besiyerinde 25 ve 35 °C lerde inkübe edilerek araştırılmıştır. 1.5 ve 5 ppm olarak pestisit ilave edilen besiyerlerine saf bakteri türleri inoküle edilerek kültür ortamı ve bakteri inoküle edilmeyen besiyeri de kontrol ortamı olarak kullanılmıştır. Hazırlanan bu ortamlar 30 gün süreyle inkübe edilmiştir. İnkübasyon süresinin farklı günlerinde (5, 10, 20, 30. gün ) kültürdeki pestisit miktarı etil asetatla (Merck) ekstrakte edilmiş ve Shimadzu marka GC 148 model gaz kromatografısi / Azot -Fosfor Dedektörü (NPD) kullanılarak pestisit kalıntı miktarı analiz edilmiştir. Kültürdeki mikroorganizmaların üreme durumları, inkübasyon süresinin farklı günlerinde (5, 10, 20, 30. gün) Plate Count Agar (PCA) besiyerinde plak kültürü metodu ile belirlenmiştir. Ayrıca spektrofotometre (Coleman Junior II) ile de 550 nm dalga boyunda ölçüm yapılarak bakteri sayısı tespit edilmiştir. Chlorpyrifos-ethyl; üç bakteri tarafından da parçalanmıştır. P. aeruginosa DSM 50071 bakterileri tarafından 25 °C de, % 20.81 (1.5 ppm) ve % 53.26 (5 ppm), 35 °C de, % 33.70 (1.5 ppm) ve % 27.44 (5 ppm), B. megaterium DSM 32 tarafından 35 °C de, % 27.89 (1.5 ppm) ve % 1 1.39 (5 ppm), B. subtilis IMG 22 bakterileri tarafından 35 °C de, % 27.81 (1.5 ppm) ve % 22.55 (5 ppm) oranında parçalanmıştır. Chloridazon; üç bakteri tarafından da parçalanmıştır. P. aeruginosa DSM 50071 tarafından 25 °C de, % 60.50 (1.5 ppm) ve% 67.86 (5 ppm), 35 °C de, % 66.31 (1.5 ppm) ve % 69.54 (5 ppm), B. megaterium DSM 32 tarafından 35 °C de, % 22.80 (1.5 ppm) ve % 58.66 (5 ppm), B. subtilis IMG 22 bakterileri tarafından 35 °C de, % 53.1 1 (1.5 ppm) ve ° o 44.48 (5 ppm) oranında parçalanmıştır.pp'-DDT; P. aeruginosa DSM 50071 bakterileri tarafından 25 ve 35 °C de, B. megaterium DSM 32 ve B. subtilis IMG 22 bakterileri tarafından 35 °C de, parçalanmamıştır.In this study, degredation of pesticides used in widely Bursa region such as Chloridazon, Chlorpyrifos -ethyl and a comparative material pp'- DDT by Pseudomonas aeruginosa DSM 50071, Bacillus megaterium DSM 32 ve Bacillus subtilis IMG 22 were investigated. Degredation of pesticides by bacteria was investigated in media of Mineral Salts Yeast Extract (MSYE)and Minimal Glucose Medium (MGM), and incubated at optimum temperature 25 and 35 °C. A culture was preparad by adding 1.5 and 5 ppm pesticide and pure bacterium species. A control medium was also prepared with only pesticide and compared with the culture of pesticide and bacterium. The prepared media were incubated for 30 days. Pesticides were extracted with ethyl acetate (Merck) in various days (5,10,20 and 30 th days) during incubation period. The extract was analysed by Shimadzu model gas chromatography (GC 148) with Nitrogen - Phosphorus Dedector (NPD). Development of microorganisms in culture during different incubation periods (5,10,20 and 30th days) were determined by plate culture method in Plate Count Agar (PCA) medium. The number of bacterium was determined by spectrophotometer (Coleman Junior II) at 550 nm. Chlorpyrifos -ethyl was also degraded by each of three bacteria. It was degraded by P. aeruginosa DSM 50071 at 25 °C, % 20.81 (1.5 ppm) and % 53.26 (5 ppm), at 35 °C, % 33.70 (1.5 ppm) and % 27.44 (5 ppm), by B. megaterium DSM 32 at 35 °C, % 27.89 (1.5 ppm) and % 1 1.39 (5 ppm), and by B. subtilis IMG 22 at 35 °C % 27.81 (1.5 ppm) and % 22.55 ( 5 ppm). Chloridazon was also degraded by each of three bacteria. It was degraded by P. aeruginosa DSM 50071 at 25 °C, % 60.50 (1.5 ppm) and % 67.86 (5 ppm), at 35 °C % 66.31 (1.5 ppm) and % 69.54 (5 ppm), by B. megaterium DSM 32 at 35 °C % 22.80 (1.5 ppm) and % 58.66 (5 ppm), and by B. subtilis BVIG 22 at 35 °C % 53.1 1 (1.5 ppm) and % 44.48 (5 ppm).IV pp- DDT was undegraded by P. aeruginosa DSM 50071 at 25 and 35 °C, by B. megatehum DSM 32 and B. subtilis IMG 22 at 35 °C

Neonatal Graves' disease occurring in an infant whose mother had a thyroidectomy due to Graves' disease [Graves hastali?i nedeniyle tiroidektomi olan annenin yenido?an bebe?inde Graves hastali?i: Bir vaka takdimi]

Neonatal Graves' disease caused by fetal thyroid gland stimulation is the result of transplacental passage of maternal thyroid-stimulating hormone receptor antibodies in the second trimester of pregnancy. A mother with Graves-Basedow disease had a total thyroidectomy operation three months before a pregnancy; maternal TSH receptor antibody (TRAb) were detected at the sixth month of pregnancy. Antithyroid drug therapy was started to the 34 weeks' gestational age infant, who was diagnosed with neonatal hyperthyroidism. The condition improved after five weeks of antithyroid therapy. The pathogenesis was believed to be due to transplacental passage of maternal TRAb. This case is presented to highlight the fact that even mothers who have a total throidectomy for Graves' disease may have infants with rarely seen neonatal Graves' disease due to the presence of maternal TRAb in the circulation

A new treatment modality for fibromyalgia syndrome: Hyperbaric oxygen therapy

Fibromyalgia syndrome (FMS) is characterized by longstanding multifocal pain with generalized allodynia/hyperalgesia. There are several treatment methods but none has been specifically approved for this application. We conducted a randomized controlled study to evaluate the effect of hyperbaric oxygen (HBO) therapy in FMS (HBO group: n = 26; control group: n = 24). Tender points and pain threshold were assessed before, and after the first and fifteenth sessions of therapy. Pain was also scored on a visual analogue scale (VAS). There was a significant reduction in tender points and VAS scores and a significant increase in pain threshold of the HBO group after the first and fifteenth therapy sessions. There was also a significant difference between the HBO and control groups for all parameters except the VAS scores after the first session. We conclude that HBO therapy has an important role in managing FMS