Dendritic cells in vivo and in vitro

Dendritic cells (DC) are crucial cells of the immune system, and bridge the essential connection between innate and adaptive immunity. They reside in the periphery as sentinels where they take up antigens. Upon activation, they migrate to lymphoid organs and present there the processed antigens to T cells, thereby activating them and eliciting a potent immune response. Dendritic cells are bone marrow-derived cells, still big controversies exist about their in vivo development. In vitro, DC can be generated from multiple precursor cells, among them lymphoid and myeloid committed progenitors. Although it remains unknown how DC are generated in vivo, studying the functions of in vitro generated DC results in fundamental knowledge of the DC biology with promising applications for future medicine. Therefore, in this review, we present current protocols for the generation of DC from precursors in vitro. We will do this for the mouse system, where most research occurs and for the human system, where research concentrates on implementing DC biology in disease treatments

Agenda 21 local como herramienta de desarollo sostenible : La situación en Suiza

1999an Suitzak bere konpromisoa bete zuen, garapen iraunkorra Konstituzioan barneratu baitzuen. Herrialde hau Tokiko Agenda 21 delakoaren moduko prozesu zehatzak sustatzeko hainbat estrategia ari da probatzen. 2005eko uztailean, Suitzako udalerrien % 4 baino ez zegoen prozesu honetan sartuta; hau da, biztanleriaren % 29. Suitzako tokiko agintaritzek, sistema federala dela eta, autonomia handia izan dute aspalditik; gainera, biztanleek demokrazia zuzeneko sistema suitzarraren bidez, Europako beste herrialde batzuetan baino zuzenago parte har dezakete erabaki politikoak hartzerakoan. Epe luzera, Garapen Iraunkorreko Estrategia Nazionalaren helburua garapen iraunkorra agintari publikoen jarduera eta politika guztietan barneratzea da.In 1999, Switzerland made good its pledge by writing sustainable development into its Constitution. Different strategies and programs are going on to promote Agenda 21-like processes. In July 2005, just 4% of municipalities were involved in the process, though this does represent 29% of the population. In Switzerland, under the country¿s federal system, local authorities have for a long time enjoyed considerable autonomy and, under the Swiss system of direct democracy, citizens are able to participate more fully in all political decision-making than in other countries. In the longer term, the aim of the National Sustainable development Strategy is to include sustainable development in all the policies and activities of public authorities.En 1999, Suiza cumplió con su compromiso al incluir el desarrollo sostenible en la Constitución. Este país está ensayando diferentes estrategias para fomentar determinados procesos del estilo de la Agenda 21 Local. En julio de 2005, tan sólo un 4% de los municipios suizos se habían involucrado en este proceso, si bien representan un 29% de la población. Las autoridades locales suizas, bajo un sistema federal, han disfrutado durante mucho tiempo de una autonomía considerable. Y los ciudadanos, mediante el sistema suizo de democracia directa, pueden participar de una manera más activa que en otros países europeos en la toma de decisiones políticas. A largo plazo, el objetivo de la Estrategia Nacional de Desarrollo Sostenible es incluir el desarrollo sostenible en todas las actividades y políticas de las autoridades pública

Nonpolar optical scattering of positronium in magnesium fluoride

We report the results of the analysis of the temperature broadening of the momentum distribution of delocalized Positronium (Ps) in Magnesium Fluoride in terms of optical deformation-potential scattering model (long-wavelength optical phonons). The Ps optical deformation-potential coupling constant $D_{o}$ in MgF$_{2}$ has been determined to be $(1.8\pm0.3)\times10^{9}$ eV/cm. We also show that the Ps momentum distribution is sensitive to second-order phase transitions in those crystals where optical deformation-potential scattering is allowed in one and forbidden in another crystalline phase

Positron localization effects on the Doppler broadening of the annihilation line: Aluminum as a case study

The coincidence Doppler broadening (CDB) technique is widely used to measure one-dimensional momentum distributions of annihilation photons, with the aim of obtaining information on the chemical environment of open-volume defects. However, the quantitative analysis of CDB spectra needs to include also purely geometrical effects. A demonstration is given here, on the basis of CDB spectra measured in quenched and in deformed pure aluminum. The comparison of the experimental results with ab initio computations shows that the observed differences come from the difference in free volume seen by positrons trapped in quenched-in vacancies or in vacancylike defects associated to dislocations. The computation reproduces accurately all details of CDB spectra, including the peak near the Fermi break, which is due to the zero-point motion of the confined positron.Peer reviewe

Analysis of electron-positron momentum spectra of metallic alloys as supported by first-principles calculations

Electron-positron momentum distributions measured by the coincidence Doppler broadening method can be used in the chemical analysis of the annihilation environment, typically a vacancy-impurity complex in a solid. In the present work, we study possibilities for a quantitative analysis, i.e., for distinguishing the average numbers of different atomic species around the defect. First-principles electronic structure calculations self-consistently determining electron and positron densities and ion positions are performed for vacancy-solute complexes in Al-Cu, Al-Mg-Cu, and Al-Mg-Cu-Ag alloys. The ensuing simulated coincidence Doppler broadening spectra are compared with measured ones for defect identification. A linear fitting procedure, which uses the spectra for positrons trapped at vacancies in pure constituent metals as components, has previously been employed to find the relative percentages of different atomic species around the vacancy [A. Somoza et al. Phys. Rev. B 65, 094107 (2002)]. We test the reliability of the procedure by the help of first-principles results for vacancy-solute complexes and vacancies in constituent metals.Comment: Submitted to Physical Review B on September 19 2006. Revised version submitted on November 8 2006. Published on February 14 200

Defect Characterization in SiGe/SOI Epitaxial Semiconductors by Positron Annihilation

The potential of positron annihilation spectroscopy (PAS) for defect characterization at the atomic scale in semiconductors has been demonstrated in thin multilayer structures of SiGe (50 nm) grown on UTB (ultra-thin body) SOI (silicon-on-insulator). A slow positron beam was used to probe the defect profile. The SiO2/Si interface in the UTB-SOI was well characterized, and a good estimation of its depth has been obtained. The chemical analysis indicates that the interface does not contain defects, but only strongly localized charged centers. In order to promote the relaxation, the samples have been submitted to a post-growth annealing treatment in vacuum. After this treatment, it was possible to observe the modifications of the defect structure of the relaxed film. Chemical analysis of the SiGe layers suggests a prevalent trapping site surrounded by germanium atoms, presumably Si vacancies associated with misfit dislocations and threading dislocations in the SiGe films

AEGIS at CERN: Measuring Antihydrogen Fall

The main goal of the AEGIS experiment at the CERN Antiproton Decelerator is the test of fundamental laws such as the Weak Equivalence Principle (WEP) and CPT symmetry. In the first phase of AEGIS, a beam of antihydrogen will be formed whose fall in the gravitational field is measured in a Moire' deflectometer; this will constitute the first test of the WEP with antimatter.Comment: Presented at the Fifth Meeting on CPT and Lorentz Symmetry, Bloomington, Indiana, June 28-July 2, 201