The selective cleaning behaviour of juvenile blue-headed wrasse (Thalassoma bifasciatum) in the Caribbean

Through the removal of parasites, dead skin and mucus from the bodies of visiting reef fish (clients), cleaner fish have a significant ecosystem function in the ecology of coral reefs. Cleaners gain nutrition from these interactions and through offering a ‘service’ are afforded protection from predators. Given these benefits, it is unclear why more fish do not engage in cleaning, and why part-time cleaning strategies exist. On coral reefs, dedicated species clean throughout their life, whereas some species are facultative, employing opportunistic and/or temporary cleaning strategies. Here, we investigate the cleaning behaviour of a facultative species to assess the relative importance of this interaction to the cleaner. Using a combination of focal and event sampling from a coral reef in Tobago, we show that cleaning is not an essential food source for facultative juvenile blue-headed wrasse (Thalassoma bifasciatum), as cleaning rate was unrelated to their foraging rate on the substrate. These wrasse displayed two cleaning strategies: stationary versus wandering cleaning, with cleaning frequency being highest for stationary cleaners. A specific cleaning location facilitated increased cleaning frequency, and wrasse cleaning rate decreased as cleaner or client abundance increased. We also compared juvenile blue-headed wrasse cleaning behaviour to a resident dedicated cleaner, the sharknose goby (Elacatinus evelynae), and showed that, in comparison, juvenile wrasse clean a narrower client range, predominately cleaning three species of gregarious free-ranging surgeonfish (Acanthurus spp.). The wrasse, however, frequently approached these clients without cleaning, which suggests that their selective cleaning strategy may be driven by the acquisition of a particular parasitic food source. Juvenile blue-headed wrasse are generalist foragers, and may thus be limited in their cleaning behaviour by their nutritional requirements, the availability of a suitable cleaning site, and fish density, which ultimately means that they do not adopt more dedicated cleaning roles within the reef community

The dynamics of cleaner-client interactions

Mutualisms involve beneficial interactions between species and link every eukaryote on the planet. Despite their pervasiveness, however, it is not clear why these interactions evolved and are so ubiquitously maintained. Cleaning is an iconic mutualism and involves a cleaner species, often a fish, removing ectoparasites and other material from the body of a heterospecific (termed a client): cleaners gain a food source, whilst the clients’ health can benefit from parasite removal. This study aimed to contribute to knowledge on the evolution and persistence of mutualisms by asking: how pervasive is cleaning across the animal kingdom, and what factors can influence the pervasiveness (Chapters 2 – 4) and dynamics (Chapters 5 – 8) of the interaction at an individual, population and species level. Mutualisms are complex and dynamic interactions, and this study identified a suite of contextual factors that can influence the pervasiveness and patterns of the interaction (Chapter 2). In situ, the availability of specific food resources, spatial location and the abiotic environment influenced the occurrence of cleaning (Chapters 3 and 4). Using a long-term 8 year data set collected on cleaner-client interactions on the same Caribbean reef, Chapters 5 and 6 showed that even within the same environment, cleaning patterns can vary temporally, spatially and, across and within client identities. Chapter 6 subsequently found that the abundance and diversity of clients (cleaner choice options), consistently regulated cleaning frequencies. The importance of both cleaner and client choice in governing who interacts with whom and how, was also found, at an individual level (Chapters 7 and 8). Together, this study highlights how sensitive cleaning interactions are to changing contexts, and how their occurrence and functioning, relies on the wide abundance, diversity and variable behaviour of clients. As Chapter 4 shows, iconic cleaning interactions are not immune to breakdowns, and coral reefs are currently one of the most threatened ecosystems on Earth, experiencing sharp declines in species abundance and diversity. It is not clear whether cleaning will be maintained as a food acquisition behaviour under future environmental conditions

Late Neogene evolution of modern deep-dwelling plankton

The fossil record of marine microplankton provides insights into the evolutionary drivers which led to the origin of modern deep-water plankton, one of the largest components of ocean biomass. We use global abundance and biogeographic data, combined with depth habitat reconstructions, to determine the environmental mechanisms behind speciation in two groups of pelagic microfossils over the past 15 Myr. We compare our microfossil datasets with water column profiles simulated in an Earth system model. We show that deep-living planktonic foraminiferal (zooplankton) and calcareous nannofossil (mixotroph phytoplankton) species were virtually absent globally during the peak of the middle Miocene warmth. The evolution of deep-dwelling planktonic foraminifera started from subpolar–mid-latitude species, during late Miocene cooling, via allopatry. Deep-dwelling species subsequently spread towards lower latitudes and further diversified via depth sympatry, establishing modern communities stratified hundreds of metres down the water column. Similarly, sub-euphotic zone specialist calcareous nannofossils become a major component of tropical and sub-tropical assemblages during the latest Miocene to early Pliocene. Our model simulations suggest that increased organic matter and oxygen availability for planktonic foraminifera, and increased nutrients and light penetration for nannoplankton, favoured the evolution of new deep-water niches. These conditions resulted from global cooling and the associated increase in the efficiency of the biological pump over the last 15 Myr

CMBPol Mission Concept Study: Probing Inflation with CMB Polarization

We summarize the utility of precise cosmic microwave background (CMB) polarization measurements as probes of the physics of inflation. We focus on the prospects for using CMB measurements to differentiate various inflationary mechanisms. In particular, a detection of primordial B-mode polarization would demonstrate that inflation occurred at a very high energy scale, and that the inflaton traversed a super-Planckian distance in field space. We explain how such a detection or constraint would illuminate aspects of physics at the Planck scale. Moreover, CMB measurements can constrain the scale-dependence and non-Gaussianity of the primordial fluctuations and limit the possibility of a significant isocurvature contribution. Each such limit provides crucial information on the underlying inflationary dynamics. Finally, we quantify these considerations by presenting forecasts for the sensitivities of a future satellite experiment to the inflationary parameters.Comment: 107 pages, 14 figures, 17 tables; Inflation Working Group contribution to the CMBPol Mission Concept Study; v2: typos fixed and references adde

The Growth of Massive Galaxies Since z=2

We study the growth of massive galaxies from z=2 to the present using data from the NEWFIRM Medium Band Survey. The sample is selected at a constant number density of n=2x10^-4 Mpc^-3, so that galaxies at different epochs can be compared in a meaningful way. We show that the stellar mass of galaxies at this number density has increased by a factor of ~2 since z=2, following the relation log(M)=11.45-0.15z. In order to determine at what physical radii this mass growth occurred we construct very deep stacked rest-frame R-band images at redshifts z=0.6, 1.1, 1.6, and 2.0. These image stacks of typically 70-80 galaxies enable us to characterize the stellar distribution to surface brightness limits of ~28.5 mag/arcsec^2. We find that massive galaxies gradually built up their outer regions over the past 10 Gyr. The mass within a radius of r=5 kpc is nearly constant with redshift whereas the mass at 5-75 kpc has increased by a factor of ~4 since z=2. Parameterizing the surface brightness profiles we find that the effective radius and Sersic n parameter evolve as r_e~(1+z)^-1.3 and n~(1+z)^-1.0 respectively. The data demonstrate that massive galaxies have grown mostly inside-out, assembling their extended stellar halos around compact, dense cores with possibly exponential radial density distributions. Comparing the observed mass evolution to the average star formation rates of the galaxies we find that the growth is likely dominated by mergers, as in-situ star formation can only account for ~20% of the mass build-up from z=2 to z=0. The main uncertainties in this study are possible redshift-dependent systematic errors in the total stellar masses and the conversion from light-weighted to mass-weighted radial profiles.Comment: Accepted for publication in the Astrophysical Journal. 26 pages, 13 figures in main tex

BHPR research: qualitative1. Complex reasoning determines patients' perception of outcome following foot surgery in rheumatoid arhtritis

Background: Foot surgery is common in patients with RA but research into surgical outcomes is limited and conceptually flawed as current outcome measures lack face validity: to date no one has asked patients what is important to them. This study aimed to determine which factors are important to patients when evaluating the success of foot surgery in RA Methods: Semi structured interviews of RA patients who had undergone foot surgery were conducted and transcribed verbatim. Thematic analysis of interviews was conducted to explore issues that were important to patients. Results: 11 RA patients (9 ♂, mean age 59, dis dur = 22yrs, mean of 3 yrs post op) with mixed experiences of foot surgery were interviewed. Patients interpreted outcome in respect to a multitude of factors, frequently positive change in one aspect contrasted with negative opinions about another. Overall, four major themes emerged. Function: Functional ability & participation in valued activities were very important to patients. Walking ability was a key concern but patients interpreted levels of activity in light of other aspects of their disease, reflecting on change in functional ability more than overall level. Positive feelings of improved mobility were often moderated by negative self perception ("I mean, I still walk like a waddling duck”). Appearance: Appearance was important to almost all patients but perhaps the most complex theme of all. Physical appearance, foot shape, and footwear were closely interlinked, yet patients saw these as distinct separate concepts. Patients need to legitimize these feelings was clear and they frequently entered into a defensive repertoire ("it's not cosmetic surgery; it's something that's more important than that, you know?”). Clinician opinion: Surgeons' post operative evaluation of the procedure was very influential. The impact of this appraisal continued to affect patients' lasting impression irrespective of how the outcome compared to their initial goals ("when he'd done it ... he said that hasn't worked as good as he'd wanted to ... but the pain has gone”). Pain: Whilst pain was important to almost all patients, it appeared to be less important than the other themes. Pain was predominately raised when it influenced other themes, such as function; many still felt the need to legitimize their foot pain in order for health professionals to take it seriously ("in the end I went to my GP because it had happened a few times and I went to an orthopaedic surgeon who was quite dismissive of it, it was like what are you complaining about”). Conclusions: Patients interpret the outcome of foot surgery using a multitude of interrelated factors, particularly functional ability, appearance and surgeons' appraisal of the procedure. While pain was often noted, this appeared less important than other factors in the overall outcome of the surgery. Future research into foot surgery should incorporate the complexity of how patients determine their outcome Disclosure statement: All authors have declared no conflicts of interes

Experiences of lifestyle change among women with gestational diabetes mellitus (GDM): A behavioural diagnosis using the COM-B model in a low-income setting

Lifestyle change can reduce the risk of developing type 2 diabetes among women with prior gestational diabetes mellitus (GDM). While understanding women’s lived experiences and views around GDM is critical to the development of behaviour change interventions to reduce this risk, few studies have addressed this issue in low- and middle- income countries. The aim of the study was to explore women’s lived experiences of GDM and the feasibility of sustained lifestyle modification after GDM in a low-income setting