Dairy Productive Potential, Challenges and Production opportunities of Horro and their F1 Jersey Crossbred Cows: A Case of Guduru Livestock Production and Research Center and Its Surroundings, West Oromia, Ethiopia

A survey was conducted on 75 smallholder householders and 205 sample cows selected by random and purposive sampling with the objectives of investigating the challenges, potential and opportunities of production of indigenous Horro and their F1 Jersey cross heifers and cows. The means ± SDs of total farmland, cropland and grazing land holding of individual smallholder householders of the study area was 3.6±3.16, 2.86±2.51 and 0.81±0.89 respectively. The means and SDs of livestock species holdings were 17±11.66, 2.9±3.98, 2.9±4.83, 1.8 ±1.66 and 9.3±8.97 for cattle, sheep, goats, equine and poultry respectively. Breeding method of cattle used was within the proportion of 63.8% and 36.2% for natural mating, and for bull and AI services respectively for on-farm production, while both AI and controlled natural mating were used in the Research Center. The means±SD of NSPC for Horro and their Jersey crossbred heifers was 2.1±1.09 and 1.7±0.94 respectively, where NSPC was significantly influenced by breed of cattle at P<0.05. The overall mean ± SE milk off-take per cow per day for local and crossbred cows were 1.5±0.01 and 5.02±0.12 litres, respectively. Feeding management, breed of animal and site of production significantly influenced milk productivity at P<0.001 both in wet and dry seasons of the year

Early Growth and Reproductive Performances of Horro Cattle and thier F1 Jersey Crosses in and around Horro-Guduru Livestock Production and Research Center, Ethiopia

A cross-sectional survey, an assessment of recorded data and  measurements of different traits were conducted to determine the reproductive and early growth performances of Horro cattle and their F1 Jersey crosses of the study area. The mean(SD) total farmland, cropland and grazing land holdings of individual smallholder householders were 3.6±3.16, 2.86±2.51 and 0.81±0.89 respectively, while the mean(SD) livestock species holding were 17±11.66, 2.9±3.98,2.9 ±4.83, 1.8±1.66 and 9.3±8.97 for cattle, sheep, goats, equine and poultry respectively. Anestrous, repeat breeder, endo-metritis and dystocia were the main reproductive health problems which were found to occurs with the  proportion of 21.1%, 19.7%, 5.6% and 1.4%, while the remaining 52% attribute to non pregnancy in cows due to voluntary waiting period, postpartum period and lack of AI service. The overall mean age at first service across both production sites(on station and on farm) was 48.85 months for local Horro and 33.25 months for Horro-Jersey F1 Crosses, where as the mean (SD) NSPC for Horro and Horro-Jersey crossbred heifers was found 2.1±1.09 and 1.7±0.94 respectively. Breed of animals had high  significance effect at (P < 0.001) on AFS and AFC. The mean birth weight of Horro and Horro-Jersey crossbred calves was 17.5±2.25 and 18.2±2.03 kg respectively. Calf birth weight was significantly (P<0.001) influenced by breed and sex, season and year of birth of a calf. The mean (SD) body weight measure of Horro calves at six, twelve, eighteen and twenty four months age is found to be 69.8±31.19, 117.7±22.65, 129.4±24.82 and 150±23.56 Kg respectively. While, their respective counterpart Horro-Jersey F1 crossbred calves were found to weigh 64.42±10.18, 136.6±27.97 and 145.1±22.27 kg mean(SD) body weight at six, twelve and eighteen  months of ages respectively

Developmental succession of the microbiome of Culex mosquitoes

Background: The native microflora associated with mosquitoes have important roles in mosquito development and vector competence. Sequencing of bacterial V3 region from 16S rRNA genes across the developmental stages of Culex mosquitoes (early and late larval instars, pupae and adults) was used to test the hypothesis that bacteria found in the larval stage of Culex are transstadially transmitted to the adult stage, and to compare the microbiomes of field-collected versus laboratory-reared mosquitoes.Results: Beta diversity analysis revealed that bacterial community structure differed among three life stages (larvae, pupae and adults) of Culex tarsalis. Although only similar to 2 % of the total number of bacterial OTUs were found in all stages, sequences from these OTUs accounted for nearly 82 % of the total bacterial sequences recovered from all stages. Thorsellia (Gammaproteobacteria) was the most abundant bacterial taxon found across all developmental stages of field-collected Culex mosquitoes, but was rare in mosquitoes from laboratory-reared colonies. The proportion of Thorsellia sequences in the microbiomes of mosquito life stages varied ontogenetically with the greatest proportions recovered from the pupae of C. tarsalis and the lowest from newly emerged adults. The microbiome of field-collected late instar larvae was not influenced significantly by differences in the microbiota of the habitat due to habitat age or biopesticide treatments. The microbiome diversity was the greatest in the early instar larvae and the lowest in laboratory-reared mosquitoes.Conclusions: Bacterial communities in early instar C. tarsalis larvae were significantly more diverse when compared to late instar larvae, pupae and newly emerged adults. Some of the bacterial OTUs found in the early instar larvae were also found across developmental stages. Thorsellia dominated the bacterial communities in field-collected immature stages but occurred at much lower relative abundance in adults. Differences in microbiota observed in larval habitats did not influence bacterial community profiles of late instar larvae or adults. However, bacterial communities in laboratory-reared C. tarsalis larvae differed significantly from the field. Determining the role of Thorsellia in mosquitoes and its distribution across different species of mosquitoes warrants further investigation

Microbiota variations in Culex nigripalpus disease vector mosquito of West Nile virus and Saint Louis Encephalitis from different geographic origins

Although mosquito microbiota are known to influence reproduction, nutrition, disease transmission, and pesticide resistance, the relationship between host-associated microbial community composition and geographical location is poorly understood. To begin addressing this knowledge gap, we characterized microbiota associated with adult females of Culex nigripalpus mosquito vectors of Saint Louis Encephalitis and West Nile viruses sampled from three locations in Florida (Vero Beach, Palmetto Inland, and Palmetto Coast). High-throughput sequencing of PCR-amplified 16S rRNA genes demonstrated significant differences among microbial communities of mosquitoes sampled from the three locations. Mosquitoes from Vero Beach (east coast Florida) were dominated by uncultivated Asaia sp. (Alphaproteobacteria), whereas microbiota associated with mosquitoes collected from two mosquito populations at Palmetto (west coast Florida) sites were dominated by uncultured Spironema culicis (Spirochaetes), Salinisphaera hydrothermalis (Gammaproteobacteria), Spiroplasma (Mollicutes), uncultured Enterobacteriaceae, Candidatus Megaira (Alphaproteobacteria; Rickettsiae), and Zymobacter (Gammaproteobacteria). The variation in taxonomic profiles of Cx. nigripalpus gut microbial communities, especially with respect to dominating taxa, is a potentially critical factor in understanding disease transmission and mosquito susceptibility to insecticides among different mosquito populations

Culex pipiens development is greatly influenced by native bacteria and exogenous yeast

Culex pipiens is the most cosmopolitan mosquito of the Pipiens Assemblage. By studying the nature of interactions between this species and microorganisms common to its breeding environment we can unravel important pitfalls encountered during development. We tested the survival rate of larval stages, pupae and adults of a Cx. pipiens colony exposed to a variety of microorganisms in laboratory conditions and assessed the transmission to offspring (F1) by those organisms that secured development up to adulthood. Three complementary experiments were designed to: 1) explore the nutritional value of yeasts and other microorganisms during Cx. pipiens development; 2) elucidate the transstadial transmission of yeast to the host offspring; and 3) to examine the relevance of all these microorganisms in female choice for oviposition-substratum. The yeast Saccharomyces cerevisiae proved to be the most nutritional diet, but despite showing the highest survival rates, vertical transmission to F1 was never confirmed. In addition, during the oviposition trials, none of the gravid females was attracted to the yeast substratum. Notably, the two native bacterial strains, Klebsiella sp. and Aeromonas sp., were the preferred oviposition media, the same two bacteria that managed to feed neonates until molting into 2nd instar larvae. Our results not only suggest that Klebsiella sp. or Aeromonas sp. serve as attractants for oviposition habitat selection, but also nurture the most fragile instar, L1, to assure molting into a more resilient stage, L2, while yeast proves to be the most supportive diet for completing development. These experiments unearthed survival traits that might be considered in the future development of strategies of Cx. pipiens control. These studies can be extended to other members of the Pipiens Assemblag