Rhinebeck Central School District and Rhinebeck Teachers Association, New York State United of Teachers (NYSUT), American Federation of Teachers (AFT), AFL-CIO (2011)

Recent trends in transport and communication infrastructures have had a profound impact on the tourism industry. Despite the growing number and importance of online travel agencies and travel metasearch sites tourism literature addressing this issue is very scarce. This paper aims to examine the temporal changes of return airfares on online travel agency (OTA) and travel metasearch websites from Budapest to three short-haul destinations for fixed departure dates in high and shoulder season. The study was based on quantitative research methods using automated internet data collection and statistical analysis (ANOVA tests). During the study, we created a database containing more than 31 thousand ticket prices over a 182-day period for the three selected destinations. Empirical findings showed that no single website offered lowest airfares consistently and metasearch sites outperformed OTAs in almost any cases by offering lower airfares. Results also indicated the best time to book flights on the selected sites, airfares for shoulder season were significantly cheaper than for high season and ticket prices tend to be more expensive on Mondays and Sundays

SIMPLE ENTRAPMENT OF ALCALASE IN DIFFERENT SILICA XEROGELS USING THE TWO STEPS SOL-GEL METHOD

The present study has focused on the entrapment of Alcalase in different xerogels obtained by using various molar ratios of methyltriethoxysilane, dimethyldietoxisilane and tetraethoxysilane. Silica and their derivatives were characterized with regard to specific surface area (nitrogen adsorbtion), chemical composition (Fourier transform infrared spectroscopy (FT-IR)), weight loss upon heating (thermogravimetric analysis (TGA)) and catalytic activity

THE THERMAL ANALYSIS OF LIGHT STEEL-FRAME DWELLING HOUSES

Charting the loss of heat due to the heat bridge effect of cold-formed steel sections constituting the load-bearing structure is an important part of the thermal analysis of the building system of light steel-frame dwelling houses. The linear heat transmission coefficient, which serves as a numerical definition of the heat bridge effect, may be calculated on the basis of thermo-photos obtained from in situ thermovisual measurements. The parts of the building under examination have also been modelled through computer simulation, which also provided a value for the linear heat transmission coefficient. The satisfactory coincidence of the results of the two test methods served as a basis of the thermal development of the building system with the aid of simulation models

Entrapment of glucoamylase by sol-gel technique in PhTES/TEOS hybrid matrixes

Mesoporous silica particles were prepared by the sol-gel method from different alkoxysilane precursors and used as a host matrix for encapsulation of glucoamylase, an enzyme widely used in fermentative industry. The aim was to investigate the physico-chemical properties of the different silica powders and their effect on the enzyme kinetics. The encapsulated enzymes followed Michaelis-Menten kinetics. The Michaelis constant (KM) and the maximum rate of starch hydrolysis reaction (Vmax) were calculated according to the Michaelis-Menten and Lineweaver-Burke plots. The values of the Michaelis constant (KM) of the encapsulated enzymes were higher than those of the free enzyme. The temperature and pH infl uence on the activity of free and immobilized glucoamylase were also compared. The results of this study show that the enzymes immobilized in organic/inorganic hybrid silica matrixes (obtained by the sol-gel method), allowing the entrapped glucoamylase to retain its biological activity, are suitable for many different applications, (medicinal, clinical, analytical)

Identification of Structural Proteins of Rhizobium meliloti Temperate Phage 16-3

The structural proteins of Rhizobium meliloti temperate phage 16-3 have been analysed by means of polyacrylamide gel electrophoresis, isoelectric focusing and agarose gel electrophoresis. Five major and five minor proteins were identified and characterized with respect to their size, isoelectric point and their distribution between the head and tail of the phage particle. The synthesis of structural proteins was studied by one- and two-dimensional gel electrophoresis

PRELIMINARY STUDY FOR USING VINYLTRIACETOXYSILANE AS PRECURSOR IN ENZYME IMMOBILIZATION BASED ON SOL-GEL METHOD

During the last years, sol-gel technology has become a well-established method for the preparation of catalytic active monoliths, bulk, particles and thin films. One reason for the increasing research activities in this field is the opportunity to obtain versatile hybrid materials by incorporation of different molecules, like dyes, enzymes, whole cells, chemicals and drugs. The aim of this research was to evaluate the suitability of vinyltriacetoxysilane (VTAS) as precursor in sol-gel enzyme immobilization and the physicochemical characterization of the final products (silica xerogels)