Activation of human T cell clones through the UM4D4/CDw60 surface antigen

UM4D4 is a recently defined antigen that is expressed on ~25% of peripheral blood T cells, but on the majority of T cells in inflammatory synovial fluid. Anti-UM4D4 activates peripheral blood T cells in the presence of accessory cells and/or phorbol ester. UM4D4 has been assigned to a new antigen cluster termed CDw60. The present study examined the ability of anti-UM4D4 to activate T cell clones derived from the synovial fluid of patients with rheumatoid arthritis. UM4D4 was expressed at varying levels on both lectin-generated and antigen-specific clones, including clones of CD4+, CD8+, and CD4-CD8- phenotypes. Anti-UM4D4 used in soluble form as a single stimulus was typically mitogenic for the CD4+ and some of the CD8+ clones, but not for the CD4-CD8- clones. Phorbol ester boosted the response to anti-UM4D4 in some clones, had no effect in others, and diminished the responses in some cases. In contrast to anti-UM4D4, anti-CD3 was generally not mitogenic in soluble form, although it was mitogenic when conjugated to beads. The data show that T cell clones derived from an inflammatory T cell infiltrate can be readily activated through the UM4D4/CDw60 antigen.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/28512/1/0000309.pd

Bloom's syndrome ataxia telangiectasia

Detailed formal protocol with illustrations and extensive bibliography.UT Southwestern--Internal Medicin

Mágoas de amizade: um ensaio em antropologia das emoções

Neste ensaio, analiso a recorrência das categorias grosseria (rudeness) e ofensa (offence) no discurso sobre a amizade de um grupo de ingleses de camadas médias em Londres. Mais do que outros sentimentos ditos positivos, a menção freqüente a essas categorias aponta para uma tensão que atravessa o discurso sobre a amizade e que problematiza o espaço das amizades no conjunto mais amplo das relações sociais. Adoto, aqui, uma abordagem pragmática dentro do campo da "antropologia das emoções". Se, em um primeiro momento, os estudos priorizavam a relativização das categorias de emoções entre as culturas, verifica-se, mais recentemente, um movimento no sentido de tomar os discursos emotivos como práticas situadas em jogos de relações sociais e negociações de poder. Com isso, a emoção deixa de ser vista como experiência interna, subjetiva, para ser analisada como prática discursiva com efeitos externos, extrapolando o chamado domínio do privado.<br>In this essay, I discuss the presence of the categories rudeness and offence in the discourse on friendship produced by a group of middle class English people resident in London. The frequent reference to these categories, rather than to the so called positive emotions, reveals the tensions regarding the negotiation of personal space which crosscut this discourse, which in turn highlight the problematic space that friendship occupies within the field of social relations. This essay draws on the analytical tools of a pragmatic approach within the area known as "anthropology of emotions", developed in the last decade in the Unites States. If at first emotion concepts were mainly studied cross-culturally, nowadays they are seen as discursive practices situated within the wider field of social relations and negotiations of power. As such, emotions cease to be treated as an internal, purely subjective experience, in order to be analysed as a discursive practice with external effects on both private and public spheres