Analysis of the genetic variation for adaptation to a short thermal stress on young « Bos taurus » cattle

In the present work, an attempt was made to determine the adaptation to heat of some cattle breeds, especially French ones. A total of 582 young male and female cattle, about 14 months old and coming from 41 elementary genetic combinations analyzed in 5 different non bioclimatological experiments, were subjected to a heat stress for 8 hours during which the room temperature was increased from 18°C to 38 °C. The usual reaction were observed i.e. increase in the rectal temperature during the stress (+ 0,54 °C), increase in the respiratory rate ( X 3), increase in the sweating rate, heart rate and skin temperature. The statistical analyses were made at three stages of stress (beginning, middle, end). In all cases, a very strong environmental effect was noticed, ie effect of the year and of experimental errors in the management of the theoretical schedule involving temperature and relative hygrometry. The data adjusted for environmental effects rather clearly show the genetic variability. However, a more accurate analysis shows that it is mainly proceeded from a between group variability when defining the groups according to « animal husbandry » parameters (dairy purpose, beef purpose, and local breed group). As a matter of fact, in most cases, there was no significant variability between the reactions of the genotypes belonging to the same group, whereas this variability existed between the groups..The satisfactory performance of the local breed group clearly appeared in comparison with the group including the improved dairy or beef breeds exhibiting only minor differences in the parameters analyzed. In the discussion, emphasis in laid ov the possible influence of thermogenesis on the results. Although our findings could have been afrected by disturbing events such as emotional stress, they agree rather well with data obtained is practice on the behaviour of the local French breeds in some hot countries.Le but de ce travail est d’essayer de caractériser l’adaptation à la chaleur d’un certain nombre de races bovines, notamment françaises. Pour cela, 582 jeunes bovins, mâles et femelles, d’environ 14 mois et issus de 41 combinaisons génétiques élémentaires analysées dans 5 expérimentations différentes, à objectif non bioclimatologique, sont soumis en chambre chaude, à un stress thermique de 8 heures pendant lequel la température externe a été portée de 11) De 18°C à 38 °C. Les réactions classiques sont observées : augmentation de la température rectale au cours du stress (+ 0,54°C), augmentation du rythme respiratoire ( X 3), augmentation du taux de sudation, du rythme cardiaque, de la température de la peau. Les analyses statistiques sont effectuées à trois stades du stress (début, milieu, fin). Dans tous les cas, on note de très forts effets dûs au milieu : effet de l’année et des erreurs expérimentales dans la conduite du protocole théorique, concernant la température et l’hygrométrie relative. Sur les données ajustées pour les effets de milieu, la variabilité génétique apparaît assez clairement. Cependant une analyse plus fine montre qu’elle provient essentiellement d’une variabilité inter groupe en définissant le groupe d’après des critères « zootechniques » ou «fonctionnels » (groupe à vocation laitière, groupe à vocation bouchère, groupe des races locales). En effet, dans la grande majorité des cas, il n’apparaît pas de variabilité significative entre les réactions des génotypes rangés dans le même groupe, alors qu’elle existe entre les groupes. La bonne performance du groupe des races locales apparaît en comparaison des groupes mettant en oeuvre les races améliorées, laitières ou bouchères, qui diffèrent peu entre eux pour les critères analysés. Dans la discussion, l’accent est mis sur l’incidence probable sur les résultats des phénomènes de thermogénèse. Si nos résultats ont pu être affectés par des phénomènes parasites, notamment ceux de stress émotionnel, il n’en reste pas moins qu’ils concordent assez bien avec l’expérience pratique acquise sur le comportement des races locales françaises dans certains pays chauds

Modelling Canopy Flows over Complex Terrain

Recent studies of flow over forested hills have been motivated by a number of important applications including understanding CO22 and other gaseous fluxes over forests in complex terrain, predicting wind damage to trees, and modelling wind energy potential at forested sites. Current modelling studies have focussed almost exclusively on highly idealized, and usually fully forested, hills. Here, we present model results for a site on the Isle of Arran, Scotland with complex terrain and heterogeneous forest canopy. The model uses an explicit representation of the canopy and a 1.5-order turbulence closure for flow within and above the canopy. The validity of the closure scheme is assessed using turbulence data from a field experiment before comparing predictions of the full model with field observations. For near-neutral stability, the results compare well with the observations, showing that such a relatively simple canopy model can accurately reproduce the flow patterns observed over complex terrain and realistic, variable forest cover, while at the same time remaining computationally feasible for real case studies. The model allows closer examination of the flow separation observed over complex forested terrain. Comparisons with model simulations using a roughness length parametrization show significant differences, particularly with respect to flow separation, highlighting the need to explicitly model the forest canopy if detailed predictions of near-surface flow around forests are required

Field observations of canopy flows over complex terrain

The investigation of airflow over and within forests in complex terrain has been, until recently, limited to a handful of modelling and laboratory studies. Here, we present an observational dataset of airflow measurements inside and above a forest situated on a ridge on the Isle of Arran, Scotland. The spatial coverage of the observations all the way across the ridge makes this a unique dataset. Two case studies of across-ridge flow under near-neutral conditions are presented and compared with recent idealized two-dimensional modelling studies. Changes in the canopy profiles of both mean wind and turbulent quantities across the ridge are broadly consistent with these idealized studies. Flow separation over the lee slope is seen as a ubiquitous feature of the flow. The three-dimensional nature of the terrain and the heterogeneous forest canopy does however lead to significant variations in the flow separation across the ridge, particularly over the less steep western slope. Furthermore, strong directional shear with height in regions of flow separation has a significant impact on the Reynolds stress terms and other turbulent statistics. Also observed is a decrease in the variability of the wind speed over the summit and lee slope, which has not been seen in previous studies. This dataset should provide a valuable resource for validating models of canopy flow over real, complex terrain

Sélection laitière divergente en race bovine Normande. I. Effets sur la production de viande des taurillons

International audienc

Comparaisons entre la race mixte normande, les races spécialisées holstein canadienne et charolaise et leurs croisements. I. – Performances de croissance des mâles et des femelles

International audienc

The effect of cigarette smoke exposure on the development of inflammation in lungs, gut and joints of TNFΔARE mice

The inflammatory cytokine TNF-alpha is a central mediator in many immune-mediated diseases, such as Crohn's disease (CD), spondyloarthritis (SpA) and chronic obstructive pulmonary disease (COPD). Epidemiologic studies have shown that cigarette smoking (CS) is a prominent common risk factor in these TNF-dependent diseases. We exposed TNF Delta ARE mice; in which a systemic TNF-alpha overexpression leads to the development of inflammation; to 2 or 4 weeks of air or CS. We investigated the effect of deregulated TNF expression on CS-induced pulmonary inflammation and the effect of CS exposure on the initiation and progression of gut and joint inflammation. Upon 2 weeks of CS exposure, inflammation in lungs of TNF Delta ARE mice was significantly aggravated. However, upon 4 weeks of CS-exposure, this aggravation was no longer observed. TNF Delta ARE mice have no increases in CD4+ and CD8+ T cells and a diminished neutrophil response in the lungs after 4 weeks of CS exposure. In the gut and joints of TNF Delta ARE mice, 2 or 4 weeks of CS exposure did not modulate the development of inflammation. In conclusion, CS exposure does not modulate gut and joint inflammation in TNF Delta ARE mice. The lung responses towards CS in TNF Delta ARE mice however depend on the duration of CS exposure

Magnetic resonance imaging after most common form of concussion

<p>Abstract</p> <p>Background</p> <p>Until now there is a lack of carefully controlled studies with conventional MR imaging performed exclusively in concussion with short lasting loss of consciousness (LOC).</p> <p>Methods</p> <p>A MR investigation was performed within 24 hours and after 3 months in 20 patients who had suffered a concussion with a verified loss of consciousness of maximally 5 minutes. As a control group, 20 age- and gender matched patients with minor orthopaedic injuries had a MR investigation using the same protocol.</p> <p>Results</p> <p>In a concussion population with an average LOC duration of 1. 4 minutes no case with unequivocal intracranial traumatic pathology was detected.</p> <p>Conclusion</p> <p>An ordinary concussion with short lasting LOC does not or only seldom result in a degree of diffuse axonal injury (DAI) that is visualized by conventional MR with field strength of 1.0 Tesla (T). Analysis of earlier MR studies in concussion using field strength of 1.5 T as well as of studies with diffusion tensor MR imaging (MR DTI) reveal methodological shortcomings, in particular use of inadequate control groups. There is, therefore, a need for carefully controlled studies using MR of higher field strength and/or studies with MR DTI exclusively in common concussion with LOC of maximally 5 minutes.</p

A Bayesian approach to modelling heterogeneous calcium responses in cell populations

Calcium responses have been observed as spikes of the whole-cell calcium concentration in numerous cell types and are essential for translating extracellular stimuli into cellular responses. While there are several suggestions for how this encoding is achieved, we still lack a comprehensive theory. To achieve this goal it is necessary to reliably predict the temporal evolution of calcium spike sequences for a given stimulus. Here, we propose a modelling framework that allows us to quantitatively describe the timing of calcium spikes. Using a Bayesian approach, we show that Gaussian processes model calcium spike rates with high fidelity and perform better than standard tools such as peri-stimulus time histograms and kernel smoothing. We employ our modelling concept to analyse calcium spike sequences from dynamically-stimulated HEK293T cells. Under these conditions, different cells often experience diverse stimuli time courses, which is a situation likely to occur in vivo. This single cell variability and the concomitant small number of calcium spikes per cell pose a significant modelling challenge, but we demonstrate that Gaussian processes can successfully describe calcium spike rates in these circumstances. Our results therefore pave the way towards a statistical description of heterogeneous calcium oscillations in a dynamic environmen

Characteristics of clinical trials in rare vs. common diseases : A register-based Latvian study

Publisher Copyright: © 2018 Logviss et al. This is an open ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and eproduction in any medium, provided the original author and source are credited.Background Conducting clinical studies in small populations may be very challenging; therefore quality of clinical evidence may differ between rare and non-rare disease therapies. Objective This register-based study aims to evaluate the characteristics of clinical trials in rare diseases conducted in Latvia and compare them with clinical trials in more common conditions. Methods The EU Clinical Trials Register (clinicaltrialsregister.eu) was used to identify interventional clinical trials related to rare diseases (n = 51) and to compose a control group of clinical trials in non-rare diseases (n = 102) for further comparison of the trial characteristics. Results We found no significant difference in the use of overall survival as a primary endpoint in clinical trials between rare and non-rare diseases (9.8% vs. 13.7%, respectively). However, clinical trials in rare diseases were less likely to be randomized controlled trials (62.7% vs. 83.3%). Rare and non-rare disease clinical trials varied in masking, with rare disease trials less likely to be double blind (45.1% vs. 63.7%). Active comparators were less frequently used in rare disease trials (36.4% vs. 58.8% of controlled trials). Clinical trials in rare diseases enrolled fewer participants than those in non-rare diseases: In Latvia (mean 18.3 vs. 40.2 subjects, respectively), in the European Economic Area (mean 181.0 vs. 626.9 subjects), and in the whole clinical trial (mean 335.8 vs. 1406.3 subjects). Although, we found no significant difference in trial duration between the groups (mean 38.3 vs. 36.4 months). Conclusions The current study confirms that clinical trials in rare diseases vary from those in non-rare conditions, with notable differences in enrollment, randomization, masking, and the use of active comparators. However, we found no significant difference in trial duration and the use of overall survival as a primary endpoint.publishersversionPeer reviewe