Oral health-related quality of life among students of the Medical University in the Russian Arctic

The aim is to assess oral health-related quality of life (OHRQoL) and factors associated with OHRQoL among the students of the medical university. Methods. The cross-sectional study included 308 Russian speaking third year students aged 19-25 years from the Faculty of General Medicine, Paediatrics, Preventive Medicine and Dentistry of the Northern State Medical University (NSMU), Arkhangelsk, Russian Arctic. The Oral Health (OH) Impact Profile (OHIP-14) was used to measure OHRQoL. Information on socio-demographic factors, self-esteem of tooth and gingiva health, OH behaviour, and diet was obtained from the OH Questionnaire for adults (World Health Organization, 2013). Pearson's chi-square test and multivariable Poisson regression with robust estimate of dispersion were used for statistical analysis. Results: 33,8 % and 18,5 % of students had poor dentition and gingiva, respectively. 39,0 % of students consumed sweet pies/buns more than once a week; 39.0 % of students reported on low OHRQoL. The highest mean scores OHIP-14 were found for the dimensions "physical pain" and "psychological discomfort": 1.59 (95 % confidence interval (CI): 1.43-1.75) and 0.84 (95 % CI: 0.70-0.98), respectively. Female sex (prevalence ratio (PR) = 1.73, 95 % CI: 1.23-2.45), poor self-esteem of teeth health (PR = 2.32, 95 % CI: 1.77-3.04), and sweet pies/buns consumption more than once a week (PR = 1.39, 95 % CI: 1.07-1.80), were all significantly and independently associated with low OHRQoL. Conclusions: Relatively high prevalence of low OHRQoL was observed in the examined students. Female sex, poor self-esteem of teeth health and frequent consumption of sweet pies/buns were associated with low OHRQoL. To enhance OHRQoL in Russian students, the preventive strategies for dental diseases and diet improvement should be implemented

Charge Transport by Light-Activated Rhodopsins Determined by Electrophysiological Recordings

Electrophysiological experiments are required to determine the ion transport properties of light-activated currents from microbial rhodopsin expressing cells. The recordings set the quantitative basis for correlation with spectroscopic data and for understanding of channel gating, ion transport vectoriality, or ion selectivity. This chapter focuses on voltage-clamp recordings of channelrhodopsin-2-expressing cells, and it will describe different illumination protocols that reveal the kinetic properties of gating. While the opening and closing reaction is determined from a single turnover upon a short laser flash, desensitization of the light-gated currents is studied under continuous illumination. Recovery from the desensitized state is probed after prolonged illumination with a subsequent light activation upon different dark intervals. Compiling the experimental data will define a minimum number of states in kinetic schemes used to describe the light-gated currents in channelrhodopsins, and emphasis will be given on how to correlate the results with the different time-resolved spectroscopic experiments