The water mites (Acari: Hydrachnidia) of the standing waters of Corsica, Sardinia and Sicily: review and new data

A compilation of our present knowledge of the water mites (Acari: Hydrachnidia) adapted to life in standing waters on the three large islands in the western Mediterranean (Corsica, Sardinia and Sicily) is provided. In addition to published data, this study deals with a rich volume of new material from recent field work, mostly deriving from intermittent ponds and pools, an extremely poorly investigated yet peculiar habitat type in the Mediterranean area. Species richness of water mites reported for the standing waters of the three islands amounts to 91 species. Out of the 47 species for which we present new distributional data, Hydrachna incisa Halbert, 1903, Hydrachna leegei Koenike, 1895, Piersigia limophila Protz, 1896, Hydryphantes crassipalpis Koenike, 1914 and Piona laminata (Thor, 1900) have not been recorded previously from the Mediterranean area. Most of these species were believed to have typical North European distributions. In addition to these, a further 13 species are recorded for the first time from the area covered. In total, 11 species are new for Italy, seven more are new for Sicily, three for Sardinia and seven for Corsica. Redescriptions are given of Axonopsis complanata (Müller, 1776) (A. graeca, nov. syn), Brachypoda baderi (reported for the first time after the original description from Abruzzo, Italy, synonymization with B. mutila rejected) and B. mutila (recorded for the first time outside Algeria with certainty). For each species, information is given on habitat preference and geographical distribution; the significance of the data is discussed under perspectives of zoogeography and nature protection. The completeness of our knowledge for the three investigated island is assessed using rarefaction curves and non-parametric estimators of species richness; while Sicily can be considered fairly well known, Corsica and Sardinia require further sampling to assess their water mite diversity

The 4 K outer cryostat for the CUORE experiment: construction and quality control

The external shell of the CUORE cryostat is a large cryogen-free system designed to host the dilution refrigerator and the bolometers of the CUORE experiment in a low radioactivity environment. The three vessels that form the outer shell were produced and delivered to the Gran Sasso underground Laboratories in July 2012. In this paper, we describe the production techniques and the validation tests done at the production site in 2012.Comment: 11 pages, 13 figures; to appear in NIM

Clinically-based determination of safe DNAemia cutoff levels for preemptive therapy or human cytomegalovirus infections in solid organ and hematopoietic stem cell transplant recipients

Transplantation Centers using human cytomegalovirus (HCMV) antigenemia-based preemptive therapy will need to replace in the near future the antigenemia assay with a more standardized and automatable assay, such as a molecular assay quantifying HCMV DNA in blood (DNAemia). Thus, in view of replacing antigenemia with clinically safe cutoff values, DNAemia levels corresponding to antigenemia cutoffs guiding HCMV preemptive therapy were determined retrospectively in solid organ and hematopoietic stem cell transplant recipients (HSCTR) using an "in-house" quantitative PCR (QPCR) method. Since preemptive therapy had prevented appearance of HCMV disease in all patients tested, DNA cutoffs determined retrospectively had to be considered as safe clinically as antigenemia cutoffs used prospectively. However, in solid organ transplant recipients (SOTR), initiating preemptive therapy upon an antigenemia cutoff of 100 pp65-positive leukocytes, a DNAemia cutoff of 300,000 copies/ml blood had positive and negative predictive values of >90%, indicating that a DNAemia cutoff could achieve, in terms of prevention of HCMV disease, the same clinical results as the antigenemia cutoff. In HSCTR, initiating preemptive therapy upon first antigenemia positivity, a DNAemia cutoff of 10,000 copies/ml blood had a positive predictive value of >90%, indicating that the great majority of patients treated under the antigenemia guidance would have been treated also using this DNA cutoff. On the other hand, the negative predictive value of 28.6% indicated that two out of three HSCTR had been treated under the antigenemia guidance having the same levels of viral DNA as the untreated patients. The data suggest that a quantitative cutoff could be adopted as a guiding criterion for preemptive therapy also in HSCTR. Regression analysis allowed to determine the DNAemia (corresponding to QPCR) cutoff values for two commercial assays tested both in solid organ and HSCTR. Retrospective DNAemia cutoff values will be verified for safety in prospective trial

Characterization of defatted products obtained from the Parmigiano–Reggiano manufacturing chain: Determination of peptides and amino acids content and study of the digestibility and bioactive properties

Parmigiano–Reggiano (PR) is a worldwide known Italian, long ripened, hard cheese. Its inclusion in the list of cheeses bearing the protected designation of origin (PDO, EU regulation 510/2006) poses restrictions to its geographic area of production and its technological characteristics. To innovate the Parmigiano–Reggiano (PR) cheese manufacturing chain from the health and nutritional point of view, the output of defatted PR is addressed. Two defatting procedures (Soxhlet, and supercritical CO2 extraction) were tested, and the obtained products were compared in the composition of their nitrogen fraction, responsible for their nutritional, organoleptic, and bioactive functions. Free amino acids were quantified, and other nitrogen compounds (peptides, proteins, and non-proteolytic aminoacyl derivatives) were identified in the extracts and the mixtures obtained after simulated gastrointestinal digestion. Moreover, antioxidant and angiotensin converting enzyme (ACE) inhibition capacities of the digests were tested. Results obtained from the molecular and biofunctional characterization of the nitrogen fraction, show that both the defatted products keep the same nutritional properties of the whole cheese

Experimental and numerical analysis of supersonic blade profiles developed for highly loaded impulse type steam turbine stages

The paper describes the results of a numerical and experimental research program addressing the aerodynamic investigation on the performance of blade profiles specifically developed for application in highly loaded impulse type turbine stages. The industrial requirements driving toward the adoption of highly loaded stage solutions are presented, along with an estimation of the profiles operating parameters. Two stator vanes and one rotor blade profile have been developed and extensively tested by means of flow field measurements and schlieren visualization in a transonic blow-down wind tunnel for linear cascades. Experimental results for the relevant operating conditions are presented, providing validation data for the CFD model used for blade design and evidencing that the main goals of the design optimization procedure have been achieved

Experimental Observation of Non-Ideal Nozzle Flow of Siloxane Vapor MDM

The first experimental results from the Test-Rig for Organic Vapors (TROVA) at Politecnico di Milano are reported. The facility implements an Organic Rankine Cycle (ORC) where the expansion process takes place within a straight axis convergent-divergent nozzle, which is the simplest geometry representative of an ORC turbine blade passage. In order to reduce the required input thermal power, a batch operating mode was selected for the plant. Experimental runs with air allowed to verify the throttling valve operation and the measurement techniques, which include total pressure and temperature measurements in the settling chamber, static pressure measurements along the nozzle axis. A double-passage Schlieren technique is used to visualize the flow field in the nozzle throat and divergent section and to determine the position of shock waves within the flow field. The first experimental observation of non-ideal nozzle flows are presented for the expansion of siloxane fluid MDM (C8H24O2Si3, octamethyltrisiloxane) for vapor expansion in the close proximity of the liquid-vapor saturation curve, at relatively low pressure of operation. A supersonic flow is attained within the divergent section of the nozzle, as demonstrated by the observation of an oblique shock wave at the throat section, where a 0.1 mm recessed step is located. Schlieren visualizations are limited by the occurrence of condensation along the mirror side of the nozzle. Pressure measurements are compatible with the observed flow field

Experimental observation of non-ideal expanding flows of Siloxane MDM vapor for ORC applications

Abstract Extensive experimental results characterizing the supersonic expansion of an organic vapor in non-ideal conditions are reported in this paper for the first time. The collected data also allowed the assessment of the accuracy of Computational Fluid Dynamic (CFD) tools employed to predict the non-ideal behavior of such flows, including the consistency of thermodynamic models adopted. The investigation has been carried out on the converging-diverging nozzle test section of the Test Rig for Organic VApors (TROVA), at the Laboratory of Compressible fluid-dynamics for Renewable Energy Application (CREA) of Politecnico di Milano. Supersonic nozzle flow was chosen as the simplest one of significance for organic Rankine cycle (ORC) turbine channels. The working fluid under scrutiny is Siloxane MDM, a widely employed compound for high temperature ORCs. MDM vapor expands through the TROVA nozzle at moderate non-ideal conditions in the close proximity of the vapor saturation curve. This is the region where ORC expanders typically operate, thus proving the relevance of the investigation for the ORC community. Indeed, detailed experimental data representative of typical ORC expansions were lacking in the open literature up to date. Two different nozzle geometries, featuring exit Mach number of 2.0 and 1.5 respectively, were tested, exploring a wide range of thermodynamic inlet conditions and diverse levels of non-ideality, from moderate non-ideal state, indicated by a compressibility factor Z = Pv/RT ≃ 0.80, to dilute gas conditions, Z ≥ 0.97. Maximum operating total pressure and temperature are Pt ≃ 5 bar and T T ≃ 250 °C. The nozzle flow is characterized in terms of total pressure, total temperature, static pressure at discrete locations along the nozzle axis, and schlieren imaging. In contrast to the well known case of polytropic ideal gas, the vapor expansion through the nozzle is found to be dependent on the inlet conditions, thus proving the non-ideal character of the flow. This influence is found to be consistent with the one predicted by the quasi-1D theory coupled with simple non-ideal gas models. Experimental data at the nozzle centerline are compared with those resulting from a two-dimensional viscous CFD calculation carried out using the SU2 software suite and the improved Peng Robinson Stryjek Vera (iPRSV) thermodynamic model. A very good accordance is found, demonstrating the high accuracy of the applied tools

Decreased humoral immune response in the bronchi of rapid decliners with chronic obstructive pulmonary disease

Background: Identification of COPD patients with a rapid decline in FEV1 is of particular interest for prognostic and therapeutic reasons. Objective: To determine the expression of markers of inflammation in COPD patients with rapid functional decline in comparison to slow or no decliners. Methods: In COPD patients monitored for at least 3 years (mean ± SD: 5.8 ± 3 years) for lung functional decline, the expression and localization of inflammatory markers was measured in bronchial biopsies of patients with no lung functional decline (FEV1% + 30 ± 43 ml/year, n = 21), slow (FEV1% ml/year, − 40 ± 19, n = 14) and rapid decline (FEV1% ml/year, − 112 ± 53, n = 15) using immunohistochemistry. ELISA test was used for polymeric immunoglobulin receptor (pIgR) quantitation “in vitro”. Results: The expression of secretory IgA was significantly reduced in bronchial epithelium (p = 0.011) and plasma cell numbers was significantly reduced in the bronchial lamina propria (p = 0.017) of rapid decliners compared to no decliners. Bronchial inflammatory cell infiltration, CD4, CD8, CD68, CD20, NK, neutrophils, eosinophils, mast cells, pIgR, was not changed in epithelium and lamina propria of rapid decliners compared to other groups. Plasma cells/mm2 correlated positively with scored total IgA in lamina propria of all patients. “In vitro” stimulation of 16HBE cells with LPS (10 μg/ml) and IL-8 (10 ng/ml) induced a significant increase while H2O2 (100 μM) significantly decreased pIgR epithelial expression. Conclusion: These data show an impaired humoral immune response in rapid decliners with COPD, marked by reduced epithelial secretory IgA and plasma cell numbers in the bronchial lamina propria. These findings may help in the prognostic stratification and treatment of COPD

Gemcitabine-releasing mesenchymal stromal cells inhibit in vitro proliferation of human pancreatic carcinoma cells

BACKGROUND AIMS: Pancreatic cancer (pCa) is a tumor characterized by a fibrotic state and associated with a poor prognosis. The observation that mesenchymal stromal cells (MSCs) migrate toward inflammatory micro-environments and engraft into tumor stroma after systemic administration suggested new therapeutic approaches with the use of engineered MSCs to deliver and produce anti-cancer molecules directly within the tumor. Previously, we demonstrated that without any genetic modifications, MSCs are able to deliver anti-cancer drugs. MSCs loaded with paclitaxel by exposure to high concentrations release the drug both in vitro and in vivo, inhibiting tumor proliferation. On the basis of these observations, we evaluated the ability of MSCs (from bone marrow and pancreas) to uptake and release gemcitabine (GCB), a drug widely used in pCa treatment. METHODS: MSCs were primed by 24-h exposure to 2000 ng/mL of GCB. The anti-tumor potential of primed MSCs was then investigated by in vitro anti-proliferation assays with the use of CFPAC-1, a pancreatic tumor cell line sensitive to GCB. The uptake/release ability was confirmed by means of high-performance liquid chromatography analysis. A cell-cycle study and secretome evaluation were also conducted to better understand the characteristics of primed MSCs. RESULTS: GCB-releasing MSCs inhibit the growth of a human pCa cell line in vitro. CONCLUSIONS: The use of MSCs as a "trojan horse" can open the way to a new pCa therapeutic approach; GCB-loaded MSCs that integrate into the tumor mass could deliver much higher concentrations of the drug in situ than can be achieved by intravenous injection

Mini Nutritional Assessment May Identify a Dual Pattern of Perturbed Plasma Amino Acids in Patients with Alzheimer's Disease: A Window to Metabolic and Physical Rehabilitation?

Conflicting results about alterations of plasma amino acid (AA) levels are reported in subjects with Alzheimer's disease (AD). The current study aimed to provide more homogeneous AA profiles and correlations between AAs and cognitive tests. Venous plasma AAs were measured in 54 fasting patients with AD (37 males, 17 females; 74.63 ± 8.03 yrs; 3.2 ± 1.9 yrs from symptom onset). Seventeen matched subjects without neurodegenerative symptoms (NNDS) served as a control group (C-NNDS). Patients were tested for short-term verbal memory and attention capacity and stratified for nutritional state (Mini Nutritional Assessment, MNA). Compared to C-NNDS, patients exhibited lower plasma levels of aspartic acid and taurine (p < 0.0001) and higher 3-methylhistidine (p < 0.0001), which were independent of patients' MNA. In comparison to normonourished AD, the patients at risk of and with malnutrition showed a tendency towards lower ratios of Essential AAs/Total AAs, Branched-chain AAs/Total AAs, and Branched-chain AAs/Essential AAs. Serine and histidine were positively correlated with verbal memory and attention capacity deficits, respectively. Total AAs negatively correlated with attention capacity deficits. Stratifying patients with AD for MNA may identify a dual pattern of altered AAs, one due to AD per se and the other linked to nutritional state. Significant correlations were observed between several AAs and cognitive tests