The views of the UK public towards routine neutering of dogs and cats

Despite being routinely recommended by veterinarians, neutering of dogs and cats has both positive and negative impacts on animal welfare and is ethically problematic. We examined attitudes of a sample of the UK public towards routine neutering of dogs and cats using a questionnaire. Respondents indicated their level of agreement with statements describing welfare and ethical reasons ‘for’ and ‘against’ the neutering of male and female dogs and cats. We conducted a general linear model (GLM) analysis to investigate the effects of demographic factors on agreement scores. Respondents (n = 451) expressed views both supporting and opposing neutering. The predominant view (>80%) supported neutering, justified primarily by prevention of unwanted offspring and reproductive diseases. Around 10% of the respondents disagreed and felt that neutering should only be done for medical reasons. Men were less likely than women to support neutering (p < 0.001). Those with meat reduction diets were more likely to be against neutering (p < 0.05) and cat owners supported neutering more than non-cat owners (p < 0.05). Although the data reflected a wide range of ethical views, our findings show that the UK public generally supports the routine neutering of dogs and cats. This insight has implications for future policy-making and compliance with veterinary advice

Skin temperature reveals the intensity of acute stress

Acute stress triggers peripheral vasoconstriction, causing a rapid, short-term drop in skin temperature in homeotherms. We tested, for the first time, whether this response has the potential to quantify stress, by exhibiting proportionality with stressor intensity. We used established behavioural and hormonal markers: activity level and corticosterone level, to validate a mild and more severe form of an acute restraint stressor in hens (Gallus gallus domesticus). We then used infrared thermography (IRT) to non-invasively collect continuous temperature measurements following exposure to these two intensities of acute handling stress. In the comb and wattle, two skin regions with a known thermoregulatory role, stressor intensity predicted the extent of initial skin cooling, and also the occurrence of a more delayed skin warming, providing two opportunities to quantify stress. With the present, cost-effective availability of IRT technology, this non-invasive and continuous method of stress assessment in unrestrained animals has the potential to become common practice in pure and applied research

Measuring the impact of dietary supplementation with citrus or cucumber extract on chicken gut microbiota using 16s rRNA gene sequencing

This study investigated the effects of dietary supplements, citrus (CTS) and cucumber (CMB), on the jejunum and cecum microbiota of 14- and 28-days old broiler chickens to evaluate their impact on the gut health and assess their role as alternatives to antibiotic growth promoters (ABGPs). 16SrRNA gene sequencing revealed the overall bacterial microbiota composition was significantly affected by the gut site (p?<?0.001) but not by either of the dietary supplements, CTS and CMB, at both 14 and 28 days of age. However, as a result of Linear discriminant analysis (LDA) effect size (LEfSE), CTS dietary supplements significantly increased the counts of Lactobacillus (p?<?0.01) and decreased the counts of Enterococcus (p?<?0.01) and Clostridium (p?<?0.05) in the jejunum, whereas the counts of Blautia were increased (p?<?0.01) and Enterococcus were decreased (p?<?0.05) in the cecum at both ages. Only minor CMB effects were identified in the cecum and non in the jejunum. The use of CTS dietary supplements has been shown to be associated to the reduction of potentially pathogenic bacteria (Enterococcus and Clostridium) and to the growth of beneficial bacteria (Lactobacillus and Blautia) which are known to have positive effects on chicken health in terms of nutrients absorption, stimulation and production of short chain fatty acids (SCFAs). Therefore, this study suggests that the use of a CTS supplemented diet could promote gut health while no clear advantages have been identified with the use of CMB as a dietary supplement

Behavioural, brain and cardiac responses to hypobaric hypoxia in broiler chickens

A novel approach to pre-slaughter stunning of chickens has been developed in which birds are rendered unconscious by progressive hypobaric hypoxia. Termed Low Atmospheric Pressure Stunning (LAPS), this approach involves application of gradual decompression lasting 280 s according to a prescribed curve. We examined responses to LAPS by recording behaviour, electroencephalogram (EEG) and electrocardiogram (ECG) in individual male chickens, and interpreted these with regard to the welfare impact of the process. We also examined the effect of two temperature adjusted pressure curves on these responses. Broiler chickens were exposed to LAPS in 30 triplets (16 and 14 triplets assigned to each pressure curve). In each triplet, one bird was instrumented for recording of EEG and ECG while the behaviour of all three birds was observed. Birds showed a consistent sequence of behaviours during LAPS (ataxia, loss of posture, clonic convulsions and motionless) which were observed in all birds. Leg paddling, tonic convulsions, slow wing flapping, mandibulation, head shaking, open bill breathing, deep inhalation, jumping and vocalisation were observed in a proportion of birds. Spectral analysis of EEG responses at 2 s intervals throughout LAPS revealed progressive decreases in median frequency at the same time as corresponding progressive increases in total power, followed later by decreases in total power as all birds exhibited isoelectric EEG and died. There was a very pronounced increase in total power at 50–60 s into the LAPS cycle, which corresponded to dominance of the signal by high amplitude slow waves, indicating loss of consciousness. Slow wave EEG was seen early in the LAPS process, before behavioural evidence of loss of consciousness such as ataxia and loss of posture, almost certainly due to the fact that it was completely dark in the LAPS chamber. ECG recordings showed a pronounced bradycardia (starting on average 49.6 s into LAPS), often associated with arrhythmia, until around 60 s into LAPS when heart rate levelled off. There was a good correlation between behavioural, EEG and cardiac measures in relation to loss of consciousness which collectively provide a loss of consciousness estimate of around 60 s. There were some effects of temperature adjusted pressure curves on behavioural latencies and ECG responses, but in general responses were consistent and very similar to those reported in previous research on controlled atmosphere stunning with inert gases. The results suggest that the process is humane (slaughter without avoidable fear, anxiety, pain, suffering and distress). In particular, the maintenance of slow wave EEG patterns in the early part of LAPS (while birds are still conscious) is strongly suggestive that LAPS is non-aversive, since we would expect this to be interrupted by pain or discomfort

Effects of analgesic intervention on behavioural responses to Low Atmospheric Pressure Stunning

Worldwide, more than 50 billion chickens are killed annually for food production so their welfare at slaughter is an important concern. Low Atmospheric Pressure Stunning (LAPS) is a novel approach to pre-slaughter stunning of poultry in which birds are rendered unconscious by gradually reducing oxygen tension in the atmosphere to achieve a progressive anoxia (hypobaric hypoxia). Advantages of this approach over electrical stunning are that birds are not shackled while conscious and all birds are reliably and irreversibly stunned. However, concerns remain that birds undergoing LAPS could experience discomfort or pain. Here we investigated whether subjecting birds to LAPS with and without administration of an opioid analgesic (butorphanol) affected behavioural responses. A blocking design was used in which pairs of birds receiving either analgesic or sham treatment were allocated to three types (analgesic/analgesic, analgesic/sham, or sham/sham). In line with previous studies, birds showed a consistent sequence of behaviours during LAPS: ataxia, loss of posture, clonic/tonic convulsions, leg paddling and motionless. Overall, administration of butorphanol had no effect on the range and patterning of behavioural responses during LAPS, but there were some differences in behaviour latencies, counts and durations. For example, latencies to ataxia, mandibulation and deep inhalation were delayed by analgesic treatment, however the duration of ataxia and other behaviours related to loss of consciousness were unaffected. Fewer birds receiving analgesia showed jumping and slow wing flapping behaviour compared to controls, which suggests these may be pain related. These behaviours after the onset of ataxia and the results may reflect a smoother induction to unconsciousness in analgised birds. Collectively, the results do not provide convincing evidence that birds undergoing LAPS are experiencing pain. While there were effects of analgesia on some aspects of behaviour, these could be explained by potential sedative, dysphoric and physiological side effects of butorphanol. The behavioural responses to LAPS appear to be primarily related to exposure to anoxia rather than hypobaric conditions, and thus in terms of welfare, this stunning method may be equivalent to controlled atmosphere stunning with inert gases

The views of the UK public towards routine neutering of dogs and cats

Despite being routinely recommended by veterinarians, neutering of dogs and cats has both positive and negative impacts on animal welfare and is ethically problematic. We examined attitudes of a sample of the UK public towards routine neutering of dogs and cats using a questionnaire. Respondents indicated their level of agreement with statements describing welfare and ethical reasons ‘for’ and ‘against’ the neutering of male and female dogs and cats. We conducted a general linear model (GLM) analysis to investigate the effects of demographic factors on agreement scores. Respondents (n = 451) expressed views both supporting and opposing neutering. The predominant view (&gt;80%) supported neutering, justified primarily by prevention of unwanted offspring and reproductive diseases. Around 10% of the respondents disagreed and felt that neutering should only be done for medical reasons. Men were less likely than women to support neutering (p &lt; 0.001). Those with meat reduction diets were more likely to be against neutering (p &lt; 0.05) and cat owners supported neutering more than non-cat owners (p &lt; 0.05). Although the data reflected a wide range of ethical views, our findings show that the UK public generally supports the routine neutering of dogs and cats. This insight has implications for future policy-making and compliance with veterinary advice</p

Body surface temperature of rats reveals both magnitude and sex differences in the acute stress response

Understanding how biological markers of stress relate to stressor magnitude is much needed and can be used in welfare assessment. Changes in body surface temperature can be measured using infrared thermography (IRT) as a marker of a physiological response to acute stress. While an avian study has shown that changes in body surface temperature can reflect the intensity of acute stress, little is known about surface temperature responses to stressors of different magnitudes and its sex-specificity in mammals, and how they correlate with hormonal and behavioural responses. We used IRT to collect continuous tail and eye temperature measurements of male and female rats (Rattus norvegicus), for 30 minutes after exposure to one of three stressors (small cage, encircling handling or rodent restraint cone) for one minute, and cross-validated the thermal response with plasma corticosterone (CORT) and behavioural assessment. To obtain individual baseline temperatures and thermal responses to stress, rats were imaged in a test arena (to which they were habituated) for 30 seconds before and 30 minutes after being exposed to the stressor. In response to the three stressors, tail temperature initially decreased and then recovered to, or overshot the baseline temperature. Tail temperature dynamics differed between stressors; being restrained in the small cage was associated with the smallest drop in temperature, in male rats, and the fastest thermal recovery, in both sexes. Increases in eye temperature only distinguished between stressors early in the response and only in females. The post stressor increase in eye temperature was greater in the right eye of males and the left eye of females. In both sexes encircling was associated with the largest increase in CORT. These results were in line with observed behavioural changes, with greater movement in rats exposed to the small cage and higher immobility after encircling. The female tail and eye temperature, as well as the CORT concentrations did not return to pre-stressor levels in the observation period, in conjunction with the greater occurrence of escape-related behaviours in female rats. These results suggest that female rats are more vulnerable to restraint stress compared to male rats and emphasise the importance of using both sexes in future investigations of stressor magnitude. This study demonstrates that stress induced changes in mammalian surface temperature measured with IRT relate to the magnitude of restraint stress, indicate sex differences and correlate with hormonal and behavioural responses. Thus, IRT has the potential to become a non-invasive method of continuous welfare assessment in unrestrained mammals.<br/

Body surface temperature of rats reveals both magnitude and sex differences in the acute stress response

Understanding how biological markers of stress relate to stressor magnitude is much needed and can be used in welfare assessment. Changes in body surface temperature can be measured using infrared thermography (IRT) as a marker of a physiological response to acute stress. While an avian study has shown that changes in body surface temperature can reflect the intensity of acute stress, little is known about surface temperature responses to stressors of different magnitudes and its sex-specificity in mammals, and how they correlate with hormonal and behavioural responses. We used IRT to collect continuous surface temperature measurements of tail and eye of adult male and female rats (Rattus norvegicus), for 30 minutes after exposure to one of three stressors (small cage, encircling handling or rodent restraint cone) for one minute, and cross-validated the thermal response with plasma corticosterone (CORT) and behavioural assessment. To obtain individual baseline temperatures and thermal responses to stress, rats were imaged in a test arena (to which they were habituated) for 30 seconds before and 30 minutes after being exposed to the stressor. In response to the three stressors, tail temperature initially decreased and then recovered to, or overshot the baseline temperature. Tail temperature dynamics differed between stressors; being restrained in the small cage was associated with the smallest drop in temperature, in male rats, and the fastest thermal recovery, in both sexes. Increases in eye temperature only distinguished between stressors early in the response and only in females. The post stressor increase in eye temperature was greater in the right eye of males and the left eye of females. In both sexes encircling may have been associated with the fastest increase in CORT. These results were in line with observed behavioural changes, with greater movement in rats exposed to the small cage and higher immobility after encircling. The female tail and eye temperature, as well as the CORT concentrations did not return to pre-stressor levels in the observation period, in conjunction with the greater occurrence of escape-related behaviours in female rats. These results suggest that female rats are more vulnerable to acute restraint stress compared to male rats and emphasise the importance of using both sexes in future investigations of stressor magnitude. This study demonstrates that acute stress induced changes in mammalian surface temperature measured with IRT relate to the magnitude of restraint stress, indicate sex differences and correlate with hormonal and behavioural responses. Thus, IRT has the potential to become a non-invasive method of continuous welfare assessment in unrestrained mammals

Body surface temperature of rats reveals both magnitude and sex differences in the acute stress response

Understanding how biological markers of stress relate to stressor magnitude is much needed and can be used in welfare assessment. Changes in body surface temperature can be measured using infrared thermography (IRT) as a marker of a physiological response to acute stress. While an avian study has shown that changes in body surface temperature can reflect the intensity of acute stress, little is known about surface temperature responses to stressors of different magnitudes and its sex-specificity in mammals, and how they correlate with hormonal and behavioural responses. We used IRT to collect continuous surface temperature measurements of tail and eye of adult male and female rats (Rattus norvegicus), for 30 minutes after exposure to one of three stressors (small cage, encircling handling or rodent restraint cone) for one minute, and cross-validated the thermal response with plasma corticosterone (CORT) and behavioural assessment. To obtain individual baseline temperatures and thermal responses to stress, rats were imaged in a test arena (to which they were habituated) for 30 seconds before and 30 minutes after being exposed to the stressor. In response to the three stressors, tail temperature initially decreased and then recovered to, or overshot the baseline temperature. Tail temperature dynamics differed between stressors; being restrained in the small cage was associated with the smallest drop in temperature, in male rats, and the fastest thermal recovery, in both sexes. Increases in eye temperature only distinguished between stressors early in the response and only in females. The post stressor increase in eye temperature was greater in the right eye of males and the left eye of females. In both sexes encircling may have been associated with the fastest increase in CORT. These results were in line with observed behavioural changes, with greater movement in rats exposed to the small cage and higher immobility after encircling. The female tail and eye temperature, as well as the CORT concentrations did not return to pre-stressor levels in the observation period, in conjunction with the greater occurrence of escape-related behaviours in female rats. These results suggest that female rats are more vulnerable to acute restraint stress compared to male rats and emphasise the importance of using both sexes in future investigations of stressor magnitude. This study demonstrates that acute stress induced changes in mammalian surface temperature measured with IRT relate to the magnitude of restraint stress, indicate sex differences and correlate with hormonal and behavioural responses. Thus, IRT has the potential to become a non-invasive method of continuous welfare assessment in unrestrained mammals