Revising the Freshwater Thelohania to Astathelohania gen. et comb. nov., and Description of Two New Species

Crayfish are common hosts of microsporidian parasites, prominently from the genus Thelohania. Thelohania is a polyphyletic genus, with multiple genetically distinct lineages found from freshwater and marine environments. Researchers have been calling for a revision of this group for over a decade. We provide evidence that crayfish-infecting freshwater Thelohania are genetically and phylogenetically distinct from the marine Thelohania (Clade V/Glugeida), whilst also describing two new species that give further support to the taxonomic revision. We propose that the freshwater Thelohania should be transferred to their own genus, Astathelohania gen. et comb. nov., in a new family (Astathelohaniidae n. fam.). This results in the revision of Thelohania contejeani (Astathelohania contejeani), Thelohania montirivulorum (Astathelohania montirivulorum), and Thelohania parastaci (Astathelohania parastaci). We also describe two novel muscle-infecting Astathelohania species, A. virili n. sp. and A. rusti n. sp., from North American crayfishes (Faxonius sp.). We used histological, molecular, and ultrastructural data to formally describe the novel isolates. Our data suggest that the Astathelohania are genetically distinct from other known microsporidian genera, outside any described family, and that their SSU rRNA gene sequence diversity follows their host species and native geographic location. The range of this genus currently includes North America, Europe, and Australia

Canadian research contributions to low vision rehabilitation: A quantitative systematic review

Purpose: Low vision rehabilitation research is a quickly growing area, due in part to the increase in the demand for services geared at older adults with age-related vision loss. Various professions collaborate to provide such rehabilitation services; however, it is currently unclear which profession takes the leading role in advancing the frontiers of low vision rehabilitation research. A recent review article proposed that in Canada, this role is held by physicians. The present study was conducted to replicate these findings under conditions of a systematic review. Method: A search of seven databases and a hand-search of four vision rehabilitation journals identified articles on low vision rehabilitation whose first author had an affiliation at a Canadian institution. Data on professional credentials, funding source, and study content was tabulated. Results: Of the 1,870 references, data from 215 eligible articles were extracted. The top four author credentials were optometrists (with or without PhD; 56 papers, 26.0%), followed by researchers with PhDs only (48 papers, 22.3%), researchers with master’s degrees (43 papers, 20.0%), and medical doctors (with or without PhD; 39 papers, 18.1%). Vision rehabilitation journals published 38 per cent of all papers, followed by ophthalmology (27%) and optometry journals (22%). Publications in the past 11 years amounted to over 50 per cent of the output over the 64-year publication history in this field in Canada, 70 per cent of which were based in universities. Conclusion: The results reflect the mosaic structure of low vision rehabilitation research in Canada, highlighting collaborations among researchers, clinicians, funding sources and rehabilitation agencies. Given its multidisciplinary nature, low vision rehabilitation research seems to be driven by collaboration among the professions

Alternosema astaquatica n. sp. (Microsporidia: Enterocytozoonida), a systemic parasite of the crayfish Faxonius virilis

Crayfish have strong ecological impacts in freshwater systems, yet our knowledge of their parasites is limited. This study describes the first systemic microsporidium (infects multiple tissue types) Alternosema astaquatica n. sp. (Enterocytozoonida) isolated from a crayfish host, Faxonius virilis, using histopathology, transmission electron microscopy, gene sequencing, and phylogenetics. The parasite develops in direct contact with the host cell cytoplasm producing mature spores that are monokaryotic and ellipsoid in shape. Spores have 9–10 coils of the polar filament and measure 3.07 ± 0.26 µm (SD) in length and 0.93 ± 0.08 µm (SD) in width. Our novel isolate has high genetic similarity to Alternosema bostrichidis isolated from terrestrial beetles; however, genetic data from this parasite is restricted to a small fragment (396 bp) of the SSU gene. Additional data related to spore morphology and development, host, environment, and ecology indicate that our novel isolate is distinct from A. bostrichidis, which supports a new species description. Alternosema astaquatica n. sp. represents a novel member of the Orthosomella-like group which appears to be a set of opportunists within the Enterocytozoonida. The presence of this microsporidium in F. virilis could be relevant for freshwater ecosystems across this crayfish's broad geographic range in North America and may affect interactions between F. virilis and invasive rusty crayfish Faxonius rusticus in the Midwest USA.</p

Behavioral Approaches for Infant Pain Relief

Infants experience a host of painful medical procedures including heel sticks, venipuncture, and immunizations. Historically, little attention was given to infant pain management due to misconceived myths and beliefs about the experience and long-term effects of pain in infants. Recent data suggest that there are both short- and long-term negative repercussions associated with unmanaged infant pain. In response, a number of non-pharmacological approaches have been developed to provide infants with pain relief associated with medical events. This paper will review these behavioral approaches including positioning, parent training, distraction, sucrose, and skin-to-skin contact. Recommendations for best practices will be provided and future direction for infant pain management will be outlined

Psychological Approaches to Acute Pediatric Pain Management

Children endure numerous acute painful events, most of which occur within the medical arena. For instance, by the time a child reaches the age of 6, the child will have experienced approximately 30 immunization injections (Centers for Disease Control and Prevention, 2008). Grounded in the Gate Control Theory (Melzack & Wall, 1965), psychological methods of pain management have focused on anxiety and pain management via behavioral means. In addition, ample research has been devoted to how best to prepare children and their parents for upcoming painful or distress-provoking procedures (e.g., surgery, hospitalization, injection). This paper will review the preparation literature, which details how to inform and arm children and their parents for impending procedures and will highlight the psychological pain management literature which includes distraction, cognitive behavioral therapy, and additional promising interventions

Cambaraspora faxoni n. sp. (Microsporidia: Glugeida) from native and invasive crayfish in the USA and a novel host of Cambaraspora floridanus

Crayfishes are among the most widely introduced freshwater taxa and can have extensive ecological impacts. Knowledge of the parasites crayfish harbor is limited, yet co-invasion of parasites is a significant risk associated with invasions. In this study, we describe a novel microsporidium, Cambaraspora faxoni n. sp. (Glugeida: Tuzetiidae), from two crayfish hosts in the Midwest USA, Faxonius virilis and Faxonius rusticus. We also expand the known host range of Cambaraspora floridanus to include Procambarus spiculifer. Cambaraspora faxoni infects muscle and heart tissue of F. rusticus and develops within a sporophorous vesicle. The mature spore measures 3.22 ± 0.14 μm in length and 1.45 ± 0.13 μm in width, with 8–9 turns of the polar filament. SSU sequencing indicates the isolates from F. virilis and F. rusticus were identical (100%) and 93.49% similar to C. floridanus, supporting the erection of a new species within the Cambaraspora genus. The novel parasite was discovered within the native range of F. rusticus (Ohio, USA) and within a native congeneric (F. virilis) in the invasive range of F. rusticus (Wisconsin, USA). Faxonius virilis is invasive in other regions. This new parasite could have been introduced to Wisconsin with F. rusticus or it may be a generalist species with a broad distribution. In either case, this parasite infects two crayfish species that have been widely introduced to new drainages throughout North America and could have future effects on invasion dynamics or impacts.</p

Arthropods infesting small mammals (Insectivora and Rodentia) near Cedar Point Biological Station in southwestern Nebraska

Cedar Point Biological Station (CPBS) is located in the mixed grass prairie of the central Great Plains, at the transition between the subregions known as the “tall grass” and “short grass” prairies. Adding to the habitat diversity, there are wetlands and riparian habitats associated with the North Platte River and the edge of the Sandhills region of north central Nebraska. This concurrence of habitats supports a diverse small mammal community. The purpose of this paper is to assemble all published information on ectoparasites associated with small mammals (Insectivora, Rodentia) of southwestern Nebraska, and to report the results of an intensive survey carried out by students of the Parasitology field course during two summers at CPBS. In 2012 and 2013, 27 species of mammal-associated arthropods were collected, including five species of sucking lice (Anoplura), a chewing louse (Ischnocera), six species of fleas (Siphonaptera), thirteen species of mesostigmatic mites (Laelapidae, Macronyssidae, Macrochelidae), and two species of metastigmatic ticks (Ixodidae). These specimens were brushed from the pelage of 11 species of small mammals that were captured in a variety of habitats around CPBS. The arthropod list includes 17 new records for the State of Nebraska. This collection is housed in the Harold W. Manter Laboratory of Parasitology (HWML), University of Nebraska State Museum, at the University of Nebraska-Lincoln, and serves as a taxonomic base for our continued efforts to establish a long-term catalog of parasites associated with small mammals in southwestern Nebraska

North American crayfish harbour diverse members of the Nudiviridae

Three novel crayfish-infecting nudiviruses from crayfish in North America represent the first genomic confirmation of nudiviruses in crayfish: Faxonius propinquus nudivirus (FpNV), Faxonius rusticus nudivirus (FrNV),and Faxonius virilis nudivirus (FvNV). Histopathology and electron microscopy revealed nuclear infections,including nuclear hypertrophy in hepatopancreatic epithelial cells and the presence of membrane-bound bacilliform virions. Metagenomic sequencing resulted in complete circular genome assembly, and phylogenetic analyses (based on nudivirus core genes) placed these viruses within the unofficial Epsilonnudivirus genus. One ofthe nudiviruses was detected in the antennal gland of its host, and another is correlated with invasive crayfishdecline in one infected lake ecosystem - suggesting a potential route for viral transmission through water, andpossible population level impact. This study highlights the importance of genomic and ecological data inelucidating the diversity and evolutionary relationships of the Nudiviridae, while expanding their known diversityand range of host species

Adherence to the Planetary Health Diet Index and Correlation with Nutrients of Public Health Concern: An analysis of NHANES 2003-2018:Planetary Health Diet Index: Trends in the US

Background: The Planetary Health Diet Index (PHDI) is a novel measure adapted to quantify alignment with the dietary evidence presented by the EAT-Lancet Commission on Food, Planet Health.Objectives: To examine how population-level health and sustainability of diet as measured by the PHDI changed from 2003-2018, and to assess how PHDI correlated with inadequacy for nutrients of public health concern (iron, calcium, potassium, and fiber) in the US.Methods: We estimated survey-weighted trends in PHDI scores and median intake of PHDI components in a nationally-representative sample of 33,859 adults aged 20+ years from eight cycles (2003–2018) of the National Health and Nutrition Examination Survey with two days of dietary recall data. We used the NCI method to examine how PHDI correlated with inadequate intake of iron, calcium, potassium, and fiber.Results: Out of a theoretical range of 0 to 140, median PHDI value increased by 4.2 points over the study period, from 62.7 (95% CI: 62.0, 63.4) points in 2003-2004 to 66.9 (66.2, 67.7) points in 2017-2018 (ptrend&lt;0.001), although most of this change occurred before 2011-2012 and plateaued thereafter. For adequacy components that are encouraged for consumption, non starchy vegetable intake significantly decreased over time, while whole grains, nuts and seeds, and unsaturated oils increased. For moderation components with recommended limits for consumption, poultry and egg intake increased, but red and processed meat, added sugars, saturated fats, and starchy vegetables decreased over time. Higher PHDI values were associated with lower probability of iron, fiber, and potassium inadequacy.Conclusions: Although there have been positive changes over the past 20 years, there is substantial room for improving the health and sustainability of the US diet. Shifting diets towards EAT-Lancet recommendations would improve nutrient adequacy for iron, fiber and potassium. Policy action is needed to support healthier, more sustainable diets in the US and globally

Dietary quality and cardiometabolic indicators in the USA: A comparison of the Planetary Health Diet Index, Healthy Eating Index-2015, and Dietary Approaches to Stop Hypertension

Background. The Planetary Health Diet Index (PHDI) measures adherence to the sustainable dietary guidance proposed by the EAT-Lancet Commission on Food, Planet, Health. To justify incorporating sustainable dietary guidance such as the PHDI in the US, the index needs to be compared to health-focused dietary recommendations already in use. The objectives of this study were to compare the how the Planetary Health Diet Index (PHDI), the Healthy Eating Index34 2015 (HEI-2015) and Dietary Approaches to Stop Hypertension (DASH) relate to cardiometabolic risk factors.Methods and Findings. Participants from the National Health and Nutrition Examination Survey (2015-2018) were assigned a score for each dietary index. We examined disparities in dietary quality for each index. We used linear and logistic regression to assess the association of standardized dietary index values with waist circumference, blood pressure, HDL-C, fasting plasma glucose (FPG) and triglycerides (TG). We also dichotomized the cardiometabolic indicators using the cutoffs for the Metabolic Syndrome and used logistic regression to assess the relationship of the standardized dietary index values with binary cardiometabolic risk factors. We observed diet quality disparities for populations that were Black, Hispanic, low-income, a low-education. Higher diet quality was associated with improved continuous and binary cardiometabolic risk factors, although higher PHDI was not associated with high FPG and was the only index associated with lower TG. These patterns remained consistent in sensitivity analyses.Conclusions. Sustainability-focused dietary recommendations such as the PHDI have similar cross-sectional associations with cardiometabolic risk as HEI-2015 or DASH. Health-focused dietary guidelines such as the forthcoming 2025-2030 Dietary Guidelines for Americans can consider the environmental impact of diet and still promote cardiometabolic health