Laboratory simulation of captan residues degradation during apple processing

The degradation of captan residues during the processing of apple to sterilised purée was investigated using laboratory small-scale processing (125 °C for 20 min and pH 4.0). [14C]-cyclohexene ring-labelled captan was completely degraded, mainly to tetrahydrophthalimide (96.5%). Other minor products such as tetrahydrophthalic acid (0.3) and tetrahydrophthalamic acid (0.2) were identified by HPLC and mass spectrometry. [14C]-trichloromethylthio-labelled captan was completely degraded essentially to [14C]CO2 (77%) accompanied by small amounts of [14C]CS2 (2%). Thiophosgene was not detectable. Approximately 11.5% of the radioactivity was non-extractable and was believed to result from the reaction of the trichloromethylthio moeity with endogenous compounds of the apple, e.g. protein. The results were compared with those obtained in buffer medium mimicking the same process

Modification of macroporous titanium tracheal implants with biodegradable structures: tracking in vivo integration for determination of optimal in situ epithelialization conditions.

Previously, we showed that macroporous titanium implants, colonized in vivo together with an epithelial graft, are viable options for tracheal replacement in sheep. To decrease the number of operating steps, biomaterial-based replacements for epithelial graft and intramuscular implantation were developed in the present study. Hybrid microporous PLLA/titanium tracheal implants were designed to decrease initial stenosis and provide a surface for epithelialization. They have been implanted in New Zealand white rabbits as tracheal substitutes and compared to intramuscular implantation samples. Moreover, a basement membrane like coating of the implant surface was also designed by Layer-by-Layer (LbL) method with collagen and alginate. The results showed that the commencement of stenosis can be prevented by the microporous PLLA. For determination of the optimum time point of epithelialization after implantation, HPLC analysis of blood samples, C-reactive protein (CRP), and Chromogranin A (CGA) analyses and histology were carried out. Following 3 weeks the implant would be ready for epithelialization with respect to the amount of tissue integration. Calcein-AM labeled epithelial cell seeding showed that after 3 weeks implant surfaces were suitable for their attachment. CRP readings were steady after an initial rise in the first week. Cross-linked collagen/alginate structures show nanofibrillarity and they form uniform films over the implant surfaces without damaging the microporosity of the PLLA body. Human respiratory epithelial cells proliferated and migrated on these surfaces which provided a better alternative to PLLA film surface. In conclusion, collagen/alginate LbL coated hybrid PLLA/titanium implants are viable options for tracheal replacement, together with in situ epithelialization.journal articleresearch support, non-u.s. gov't2012 Aug2012 03 02importe

The CADM1 tumor suppressor gene is a major candidate gene in MDS with deletion of the long arm of chromosome 11.

Myelodysplastic syndromes (MDS) represent a heterogeneous group of clonal hematopoietic stem cell disorders characterized by ineffective hematopoiesis leading to peripheral cytopenias and in a substantial proportion of cases to acute myeloid leukemia. The deletion of the long arm of chromosome 11, del(11q), is a rare but recurrent clonal event in MDS. Here, we detail the largest series of 113 cases of MDS and myelodysplastic syndromes/myeloproliferative neoplasms (MDS/MPN) harboring a del(11q) analyzed at clinical, cytological, cytogenetic, and molecular levels. Female predominance, a survival prognosis similar to other MDS, a low monocyte count, and dysmegakaryopoiesis were the specific clinical and cytological features of del(11q) MDS. In most cases, del(11q) was isolated, primary and interstitial encompassing the 11q22-23 region containing ATM, KMT2A, and CBL genes. The common deleted region at 11q23.2 is centered on an intergenic region between CADM1 (also known as Tumor Suppressor in Lung Cancer 1) and NXPE2. CADM1 was expressed in all myeloid cells analyzed in contrast to NXPE2. At the functional level, the deletion of Cadm1 in murine Lineage-Sca1+Kit+ cells modifies the lymphoid-to-myeloid ratio in bone marrow, although not altering their multilineage hematopoietic reconstitution potential after syngenic transplantation. Together with the frequent simultaneous deletions of KMT2A, ATM, and CBL and mutations of ASXL1, SF3B1, and CBL, we show that CADM1 may be important in the physiopathology of the del(11q) MDS, extending its role as tumor-suppressor gene from solid tumors to hematopoietic malignancies

Etalonnage d'un réseau d'antennes

L'invention concerne un système antennaire compor¬tant au moins une antenne (12) reliée à un récepteur (2) de traitement de signaux captés, un radôme (3) de protection de l'antenne ; et plusieurs sondes électro-optiques (42) réparties sur ou dans le radôme

Optically modulated scatterer probe for in-situ antenna monitoring

International audienceTraditionally antenna arrays are optimized and measured under ideal conditions usually in anechoic chamber. However, when the antenna is placed in a real environment (in the presence of near field scatterers), its radiation characteristics can be significantly perturbed compared to the ideal case. This paper presents a non-invasive solution to monitor antenna radiated performance with an embedded diagnostic system. The Optically Modulated Scatterer Technique is implemented. The performances of the OMS probe are analyzed both with static and dynamic characterization

Experimental validation of power budget model of optically modulated scatterer technique

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