HUBUNGAN ANTARA PENGAWASAN ATASAN DAN PENGETAHUAN DENGAN TINDAKAN PENGGUNAAN ALAT PELINDUNG DIRI (APD) PADA PERAWAT DI RUMAH SAKIT UMUM DAERAH (RSUD) MARIA WALANDA MARAMIS KABUPATEN MINAHASA UTARA

Tenaga kerja berisiko tinggi terinfeksi penyakit yang dapat mengancam keselamatannya saat bekerja . Perawat merupakan tenaga kerja yang lebih banyak kontak langsung dengan pasien oleh karena itu perawat harus menerapkan penggunaan Alat Pelindung Diri (APD) sesuai dengan Standart Operating Procedure (SOP) untuk menghindari terjadinya infeksi saat bekerja.Penelitian ini dilakukan untuk mengetahui hubungan antara pengawasan atasan dan pengetahuan dengan tindakan penggunaan Alat Pelindung Diri (APD) pada perawat di rumah sakit umum Daerah (RSUD) Maria Walanda Maramis Kabupaten Minahasa Utara. Penelitian ini merupakan survey analitik dengan pendekatan cross-sectional  (potong lintang). Penelitian ini dilaksanakan pada bulan April - Mei 2019. Populasi pada penelitian ini adalah semua perawat yang ada di Rumah Sakit Umum Daerah (RSUD) Maria Walanda Maramis Kabupaten Minahasa Utara berjumlah 64 orang perawat. Analisis Bivariat menggunakan uji Spearman rank ɑ = 0,05 tingkat kemaknaan 95%. Hubungan antara pengawasan atasan dengan tindakan penggunaan alat pelindung diri didapatkan nilai probabilitas pvalue = 0,005 dan r = 0,346. Hubungan antara pengetahuan dengan tindakan penggunaan alat pelindung diri di dapatkan nilai probabilitas pvalue = 0.000 dan r =0,491. Terdapat hubungan antara pengawasan atasan dengan tindakan penggunaan alat pelindung diri pada perawat di Rumah Sakit Umum Daerah (RSUD) Maria Walanda Maramis Kabupaten Minahasa Utara. Terdapat hubungan antara pengetahuan dengan tindakan penggunaan alat pelindung diri pada perawat di Rumah Sakit Umum Daerah (RSUD) Maria Walanda Maramis Kabupaten Minahasa Utara. Kata Kunci :Pengawasan Atasan, Pengetahuan, Tindakan penggunaan APD ABSTRACKWorkers are at high risk of contacting with infection diseases that can threaten their safety at work. Nurses are  worelikely to have direct contact with patients, there for they need to utilised the Personal Protective Equipment (PPE) in accordance with the Standard Operating Procedure (SOP) to avoid infection at work. This research was conducted to determine the relationship between supervisor supervision and knowledge of  PPE with the act of using PPE on  nurses at the Regional General Hospital Maria Walanda Maramis, North Minahasa Regency. This research was an analytical survey with a cross-sectional study design. This research was conducted in  April to May 2019. Population in this research was 64 nurses in the Regional General Hospital Maria Walanda Maramis Kabupaten Minahasa Utara. Bivariate analysis used Spearman Rank Test (ɑ = 0.05, CI 95%). Correlation between supervision with the action of used personal protective equipment (ppe) probability values obtained that pvalue=0.005 and r=0.346. Correlation between knowledge with the action of used PPE probability values obtained that pvalue=0.000 and r=0.491. The conclusion that there are correlation between supervision with the action of used PPE and there are correlation between knowledge with the action of used PPE in nurses to the Regional General Hospital Maria Walanda Maramis Kabupaten Minahasa Utara. Keywords : Supervision, Knowledge, The Action Of Using Personal Protective Equipment (PPE)

Hubungan Antara Beban Kerja Dengan Stres Kerja Pada Perawat Pelaksana Di Ruang Isolasi Covid-19 Rumah Sakit Umum Daerah Kota Kotamobagu

Dampak dari wabah Coronavirus Disease of 2019 (COVID-19) menimbulkan pengaruh yang serius pada tenaga kesehatan. Dampak yang paling berpengaruh yaitu kesehatan, dampak kesehatan yang ditimbulkan bukan hanya kesehatan secara fisik namun dapat memiliki efek serius pada kesehatan mental dan merujuk untuk munculnya keluhan stres kerja khususnya petugas kesehatan atau perawat. Kasus COVID-19 tidak bisa diprediksi, maka perlu adanya perkembangan ilmu kesehatan dalam penanganan COVID-19 khususnya pada perawat. Penelitian ini bertujuan untuk mengetahui Hubungan antara Beban Kerja dengan Stres Kerja pada Perawat Pelaksana di Ruang Isolasi COVID-19 Rumah Sakit Umum Daerah Kota Kotamobagu. Jenis penelitian kuantitatif dengan menggunakan metode analitik observasional dengan pendekatan studi potong lintang Cross-Sectional, dilaksanakan di RSUD Kota Kotamobagu pada bulan Agustus - Oktober tahun 2021. Sampel sebanyak 62 responden dengan menggunakan teknik pengambilan sampel yaitu total sampling. Instrumen dalam penelitian ini menggunakan kuesioner. Penelitian ini di ikuti oleh 62 responden dimana 53% berumur  28 tahun, mayoritas perempuan 71,6% dengan tingkat pendidikan D3 69,4% dan masa kerja  5 tahun 74%. Pada penelitian ini ditemukan 91,9% melaporkan mengalami stres ringan dan mempunyai beban kerja ringan 75,8%. Hasil penelitian ini menggunakan uji korelasi Somers’d. Hasil yang didapatkan dalam penelitian ini yaitu berdasarkan uji statistik diperoleh nilai korelasi koefisien sebesar 0,157 yang menunjukkan korelasi yang sangat lemah dengan nilai p = 0.153>0,05 artinya tidak ada hubungan antara beban kerja dengan stres kerja pada perawat pelaksana di ruang isolasi COVID-19 RSUD Kota Kotamobagu. Kesimpulan dalam penelitian ini bahwa beban kerja bukan satu-satunya faktor penting yang mempengaruhi stres kerja pada perawat pelaksana diruang isolasi COVID-19 RSUD Kota Kotamobagu. Saran untuk instansi rumah sakit agar bisa mengevaluasi faktor-faktor lain yang bisa menyebabkan stres pada perawat. Kata kunci: stres kerja, beban kerja, perawat pelaksana ruang isolasi COVID-19 ABSTRACTThe impact of the Coronavirus Disease of 2019 (COVID-19) outbreak has a serious impact on health workers. The most influential impact is health, the health impact caused not only physical health but can have a serious effect on mental health and refer to the emergence of complaints of work stress, especially health workers or nurses. COVID-19 cases cannot be predicted, so there needs to be the development of health science in handling COVID-19, especially in nurses. This study aims to find out the relationship between workload and work stress in the managing nurse in the isolation room of COVID-19 kotamobagu regional general hospital. This type of quantitative research using observational analytical methods with cross-sectional latitude cut study approach, was carried out at Kotamobagu Hospital in August - October 2021. A sample of 62 respondents using sampling techniques, namely total sampling. The instruments in the study used questionnaires. The study was followed by 62 respondents where 53% aged ≤ 28 years, the majority of women 71.6% with a D3 education level of 69.4% and a working period of ≤ 5 years 74%. In this study, 91.9% reported experiencing mild stress and having a light workload of 75.8%. The results of this study used the Somers'd correlation test. The results obtained in this study, based on statistical tests obtained a coefficient correlation value of 0.157 which showed a very weak correlation with a value of p = 0.153>0.05 means that there is no relationship between workload and work stress in the implementing nurse in the isolation room of COVID-19 Kotamobagu Hospital. The conclusion in this study that workload is not the only important factor that affects work stress in the implementing nurses in the isolation room of COVID-19 Kota Kotamobagu Hospital. Advice for hospital agencies to be able to evaluate other factors that can cause stress in nurses. Keywords: work stress, workload, COVID-19 isolation room managing nurse  

Diversity in collaborative research communities: a multicultural, multidisciplinary thesis writing group in public health

Writing groups for doctoral students are generally agreed to provide valuable learning spaces for Ph.D. candidates. Here an academic developer and the eight members of a writing group formed in a Discipline of Public Health provide an account of their experiences of collaborating in a multicultural, multidisciplinary thesis writing group. We consider the benefits of belonging to such a group for Ph.D. students who are operating in a research climate in which disciplinary boundaries are blurring and where an increasing number of doctoral projects are interdisciplinary in nature; in which both academic staff and students come from enormously diverse cultural and language backgrounds; and in which teamwork, networking and collaboration are prized but not always proactively facilitated. We argue that doctoral writing groups comprising students from diverse cultural and disciplinary backgrounds can be of significant value for postgraduates who wish to collaborate on their own academic development to improve their research writing and communication skills; at the same time, such collaborative work effectively builds an inclusive, dynamic research community.Cally Guerin, Vicki Xafis, Diana V. Doda, Marianne H. Gillam, Allison J. Larg, Helene Luckner, Nasreen Jahan, Aris Widayati and Chuangzhou X

Reduction in greenhouse gas emissions from national climate legislation

The international response to climate change has been inadequate, but not zero. There are 1,800 climate change laws worldwide. We use panel data on legislative activity in 133 countries over the period 1999–2016 to identify statistically the short-term and long-term impact of climate legislation. Each new law reduces annual carbon dioxide (CO2) emissions per unit of gross domestic product by 0.78% nationally in the short term (during the first three years) and by 1.79% in the long term (beyond three years). The results are driven by parliamentary acts and by countries with a strong rule of law. In 2016, current climate laws were associated with an annual reduction in global CO2 emissions of 5.9 GtCO2, more than the US CO2 output that year. Cumulative CO2 emissions savings from 1999 to 2016 amount to 38 GtCO2, or one year’s worth of global CO2 output. The impact on other greenhouse gases is much lower

MEG3 long noncoding RNA regulates the TGF-β pathway genes through formation of RNA-DNA triplex structures

Long noncoding RNAs (lncRNAs) regulate gene expression by association with chromatin, but how they target chromatin remains poorly understood. We have used chromatin RNA immunoprecipitation-coupled high-throughput sequencing to identify 276 lncRNAs enriched in repressive chromatin from breast cancer cells. Using one of the chromatin-interacting lncRNAs, MEG3, we explore the mechanisms by which lncRNAs target chromatin. Here we show that MEG3 and EZH2 share common target genes, including the TGF-β pathway genes. Genome-wide mapping of MEG3 binding sites reveals that MEG3 modulates the activity of TGF-β genes by binding to distal regulatory elements. MEG3 binding sites have GA-rich sequences, which guide MEG3 to the chromatin through RNA-DNA triplex formation. We have found that RNA-DNA triplex structures are widespread and are present over the MEG3 binding sites associated with the TGF-β pathway genes. Our findings suggest that RNA-DNA triplex formation could be a general characteristic of target gene recognition by the chromatin-interacting lncRNAs