Ethanol Induced Toxicity and Lipid Peroxidation in Pregnant Mice: Protective Effects of Butanolic Extract from Leaves of Chrysanthemum fontanesii, Vitamin E and C

Background: The objective of the present study was to investigate the ability of butanolic extract from leaves of Chrysanthemum fontanesii, vitamin E and C to modulate ethanol-Induced toxicity and oxidation damage in maternal and fetal tissues of mice. Butanolic extract from leaves of Chrysanthemum fontanesii (200 mg/Kg per day), vitamin E (100mg/Kg per day) and C (8.3mg/Kg per day) were administered by gavage to groups of pregnant mice from the 6 th to 17 th day of gestation. A number of animals received plant extract, vitamin E and C, also treated with an oral administration of ethanol (0.02ml/g of 25% v/v absolute ethanol in water per day) in same conditions. On day 18 of gestation, pregnant mice were killed, fetus, placenta, fetal liver, liver, kidneys and brain were removed, homogenised and used for determination of lipid peroxidation (LPO) using TBARS method. Embryotoxicity was assessed by counting the number of live and dead fetus and growth retardation. Results: Severe alterations in all biomarkers were observed after injury with ETOH. ETOH produced significant decreases in fetal weight and significant increases in embryolethality and lipid peroxidation relative to control values. Treatment with Chrysanthemum fontanesii extract, vitamin C and vitamin E resulted in markedly decreased embryolethality and fetal growth retardation, while increased fetal weight were observed. Conclusion: The butanolic extract from leaves of Chrysanthemum fontanesii, vitamin E and C protected against ethanol induce fetal and maternal toxicity as revealed by the decrease in the extent of lipid peroxidation. So that butanolic extract from leaves of Chrysanthemum fontanesii posses in vivo antioxidant properties

Diet, Lifestyles, Family History, and Prostate Cancer Incidence in an East Algerian Patient Group

Prostate cancer (PC) is the fourth most common cancer in men and the sixth leading cause of death in Algeria. To examine the relationship between lifestyle factors, including diet, and family history and PC risk, a case-control study was performed in an eastern Algerian population, comprising 90 patients with histologically confirmed PC and 190 controls. Data collection was carried out through a structured questionnaire and statistical analysis was performed to evaluate the different variables. The data showed that consumption of lamb and beef meat and high intake of animal fat and dairy products increased PC risk. Seven to thirteen vegetables servings per week and fourteen or more servings decreased PC risk by 62% and 96%, respectively. Seven to fourteen fruit servings per week decrease PC risk by 98%. Green tea consumption reduced the risk of PC but the results were statistically borderline. Increased risk was observed for individuals with family history of PC in first and in second degree. A positive strong association was also found for alcohol and smoking intake and a dose-response relationship existed for quantity and history of smoking. This study suggests that dietary habits, lifestyle factors, and family history have influence on the development of PC in Algerian population

Antioxidant activity and chemical constituents of Anthriscus vulgaris Bernh. (Apiaceae) from Algeria

AbstractThe chloroform and ethyl acetate extracts obtained from the aerial parts of Anthriscus vulgaris Bernh. were analyzed by gas chromatography-mass spectrometry (GC-MS). 36 components have been identified in each extract. The major constituents were 1-monooleoylglycerol (20.72%), caffeic acid (15.20%), cinnamic acid (11.31%) and benzene acetic acid (10.95%). The phytochemical study led to the isolation and structural elucidation of three compounds, scopoletin, umckalin and 1-(3',4'-dihydroxycinnamoyl) cyclopentane-2,3-diol. Moreover the ethyl acetate extract was screened for its possible in vitro antioxidant activity by 2,2-diphenyl-1-picrylhydrazy l(DPPH) and lipid peroxidation inhibition assays in which it displayed a noticeable activity. This study provides the first biological and chemical investigation on Anthriscus vulgaris Bernh. in Algeria

In vitro antioxidant, antibacterial and membrane stabilizing activity of plant extract from Chrysanthemum fontanesii

Antioxidant activities, antibacterial and membrane stabilizing activity of butanolic extract from leaves of Chrysanthemum fontanesii were investigated. The inhibition of the formation of malondialdehyde (MDA) in vitro and the scavenging of DPPH were assayed .The phenolic content of the extract was determined. The experimental results show that butanolic extract have antioxidant activity in vitro. The extracts showed a high antioxidant effect, especially scavenging of DPPH anions and inhibition of lipid peroxidation. Antioxidant activities were compared to ascorbic acid. The total content of phenolic compounds was 349 µg of gallic acid equivalents/mg extract. The butanolic extract was effective against microorganisms and against heat-as well as hypotonic solution-induced haemolysis of erytrocytes in vitro

Protective role of Limonium bonduelli extract against non-enzymatic peroxidation in brain and testes induced by iron in vitro.

Infertility and Neurodegerative diseases have been linked to oxidative stress arising from peroxidation of membrane biomolecules and high levels of iron have been reported to play an important role. The present study sought to determine the antioxidant activity and protective ability of n-butanol extract of Limonium bonduelli on lipid peroxidation induced by FeSO4 in rat brain and testes homogenates in vitro. n-butanol extract of the aerial parts (leaves and flowers) was prepared, and the ability of the extract to inhibit FeSO4 induced lipid peroxidation in isolated rat brain and testes was determined using spectrophotometric method. The study revealed that the extract inhibited malondialdehyde (MDA) production in FeSO4 induced lipid peroxidation in the brain and testes in a dose-dependent manner and the highest percentage of the inhibition was 89.80% similar to vitamin C in the same concentration (100 µg/mL) in brain and 82.33% in testes (200 µg/mL). Limonium bonduelli extract demonstrated important anti-lipid peroxidative effect, which may be useful in preventing the progress of various oxidative stress related diseases. The higher inhibitory effect of the extract could be attributed to its phytochemical content

Oxidative stress and brain mitochondria swelling induced by endosulfan and protective role of quercetin in rat

International audienceThe neurological damages resulted by endosulfan poisoning is not completely elucidated, especially in cellular organelles such as mitochondria. In the present study, the pro-oxidant effect of endosulfan on brain mitochondria was first investigated. Gavages of endosulfan into rats at the dose of 2 mg/kg induced oxidative stress in this organelle since it provokes a significant reduction of catalase (CAT), superoxide dismutase (SOD), and glutathione (GSH) level. In addition, a significant increase in mitochondria swelling and malondialdehyde (MDA) levels were observed in neuronal mitochondria, indicating clearly an intense peroxidation within mitochondria. Second, the protective effect of quercetin (QE) (10 mg/kg) against endosulfan-induced oxidative stress in mitochondria was also assessed. Indeed, the pretreatment of rats with QE protects brain mitochondria from oxidative stress, lipid peroxidation, and mitochondria swelling induced by endosulfan. The activities of antioxidant enzymes and the mitochondrial content of GSH and MDA were returned to control values. Thus, although endosulfan can have neurotoxic effects in brain rats, this toxicity can be prevented by quercetin

Chemical Composition, Antioxidant and Anticorrosive Activities of Hammada articulata Essential Oil

International audienceThe objective of this research was to investigate the chemical composition of the essential oil of Hammada articulata obtained by steam distillation and to investigate its antioxidant and anticorrosive activities. Gas chromatography–mass spectrometry analysis of this oil resulted in the identification of 46 compounds, which accounted for 97.0% of the total oil. The major components found in this oil were hexahydrofarnesyl acetone (11.9%), β-bisabolene (10.7%), eugenol (7.7%) and 2-methyldecalin (5.3%). The DPPH radical scavenging technique was used to assess antioxidant activity. The sample of essential oil presented good antioxidant activity. This oil was tested as a corrosion inhibitor of stainless steel in 0.5 M HCl using weight loss measurements and electrochemical polarization methods. The corrosion rate of stainless steel was decreased in the presence of essential oil. The inhibition efficiency was found to increase with oil concentration to attain 96.68% at 1000 ppm. The adsorption of inhibitor on the stainless steel surface was found to be a spontaneous process and obeys Langmuir's adsorption isotherm

Compositional study, antibacterial and antioxidant potential of Lepidium draba L. (brascicaceae)

The present study was designed to evaluate the phytochemical screening, antimicrobial and antioxidant activities of Lepidium draba The arial parts of Lepidium draba showed the presence of alkaloids, terpenoids, tannins,saponins, Leuco anthocyans, triterpenoids and flavonoids. Three known flavonoid glycosides: rhamnocitrin- 3-O- Dglucoside (1), complanatuside (2), genkwanin- 4’-O- Dglucoside (3) were isolated from the all parts of Lepidium draba their structures were elucidated by NMR experiments and acid hydrolysis. The antibacterial activity of the extracts was evaluated by disc diffusion assay and the antioxidant activity and phenolic contents of n-butanol extract were evaluated in vitro using spectrophotometer methods.Peer Reviewe

Erratum to: Neurobehavioral deficits and brain oxidative stress induced by chronic low exposure of persistent organic pollutants mixture in adult female rat

Erratum to: Neurobehavioral deficits and brain oxidative stress induced by chronic low exposure of persistent organic pollutants mixture in adult female ra

Diet, Lifestyles, Family History, and Prostate Cancer Incidence in an East Algerian Patient Group

Prostate cancer (PC) is the fourth most common cancer in men and the sixth leading cause of death in Algeria. To examine the relationship between lifestyle factors, including diet, and family history and PC risk, a case-control study was performed in an eastern Algerian population, comprising 90 patients with histologically confirmed PC and 190 controls. Data collection was carried out through a structured questionnaire and statistical analysis was performed to evaluate the different variables. The data showed that consumption of lamb and beef meat and high intake of animal fat and dairy products increased PC risk. Seven to thirteen vegetables servings per week and fourteen or more servings decreased PC risk by 62% and 96%, respectively. Seven to fourteen fruit servings per week decrease PC risk by 98%. Green tea consumption reduced the risk of PC but the results were statistically borderline. Increased risk was observed for individuals with family history of PC in first and in second degree. A positive strong association was also found for alcohol and smoking intake and a dose-response relationship existed for quantity and history of smoking. This study suggests that dietary habits, lifestyle factors, and family history have influence on the development of PC in Algerian population