Association of serum and follicular fluid leptin and ghrelin levels with in vitro fertilization success

 Objectives: The aim of this study was to evaluate the relationship between in vitro fertilization (IVF) cycle outcomes, serum and follicular fluid (FF) levels of leptin and ghrelin. Material and methods: Forty-four women who underwent intracytoplasmic sperm injection cycles (ICSI) were enrolled in the study. On the third day (D3) of the menstrual cycle, venous blood samples were drawn for serum measurements of leptin and ghrelin. The follicular fluid (FF) and the corresponding oocyte were obtained from a single dominant preovulatory follicle at the time of oocyte pick-up. The FF and D3 serum leptin and ghrelin concentrations were measured by enzyme-linked immunosorbent assay. The relationship between pregnancy rate and serum, follicular fluid levels of leptin and ghrelin were analyzed. Results: Of the 44 cases included, nineteen achieved clinical pregnancy (43.18%). Follicular fluid ghrelin levels were significantly lower in the pregnant group than non-pregnant group (p < 0.05) With respect to FF leptin, there was no statistically significant differences between the pregnant and non-pregnant women (p > 0.05). There was no statistically significant difference in D3 serum ghrelin between pregnant and non-pregnant groups (p > 0.05). However, D3 serum leptin levels were significantly lower in pregnant women than non-pregnant women (p < 0.05). Conclusions: Lower ghrelin levels in the follicular fluid were associated with higher pregnancy rates. Also, D3 serum leptin levels were inversely correlated with clinical pregnancy rates. These findings support the potential role of these molecules on IVF outcomes

ESHRE good practice recommendations for add- ons in reproductive medicine

The draft of the paper “ESHRE Good practice recommendations for add-ons in reproductive medicine” was published for public review for 4 weeks, between 1 November and 1 December 2022. This report summarizes all reviewers, their comments and the reply of the working group and is published on the ESHRE website as supporting documentation to the paper. During the stakeholder review, a total of 274 comments (including 24 duplicates) were received from 46 reviewers. Reviewers included professionals and representatives of donor-conceived offspring organisations. The comments were focussed on the content of the guideline (209 comments), language and style (31 comments), or were remarks that did not require a reply (10 comments). All comments to the language and format were checked and corrected where relevant. The comments to the content of the paper (n=209) were assessed by the working group and where relevant, adaptations were made in the paper (n=94; 45%). Adaptations included revisions and/or clarifications of the text, and amendments to the recommendations. For a number of comments, the working group considered them outside the scope of the paper or not appropriate/relevant (n=115; 55%).peer-reviewe

Testicular toxicity of Dichlorvos and the protective role of vitamins C and E in rats

Organofosfatlı bir insektisit olan dichlorvos ziraî mücadelede sıklıkla kullanılmaktadır. Bu çalısmada erkek ratlar 4 gruba ayrılmıstır. 1. grup kontrol grubu ratlar, 2. grup vitamin C (200 mg/kg gün)+vitamin E (200 mg/kg gün) muameleli ratlar, 3. grup dichlorvos (1,6 mg/kg gün) muameleli ratlar ve 4. grup vitamin C (200 mg/kg gün)+vitamin E (200 mg/kg gün)+ dichlorvos (1,6 mg/kg gün) muameleli ratlar. Maddeler ratlara oral gavaj yoluyla verilmistir. Muameleden 24 saat, 4 hafta ve 7 hafta sonra ratların vücut ve testis agırlıkları tartılmıs, testislerdeki sperm sayısı, epididimlerindeki sperm motilitesi ve morfolojisi, kandaki folikül stimüle edici hormon (FSH), lüteinlestirici hormon (LH) ve testosteron düzeyleri arastırılmıs, ısık ve elektron mikroskobuyla testis dokularındaki patolojik degisiklikler incelenmistir. Deney grubu ratlar, sperm sayısı bakımından kontrol grubu ile karsılastırıldıgında istatistiksel olarak herhangi bir fark gözlenmedi. Sperm motilitesi bakımından kontrol grubu ratlarla dichlorvos, vitamin C ve E+dichlorvos grubu ratlar karsılastırıldıgında 24 saat ve 4. hafta sonunda bir degisiklik gözlenmezken, 7. haftanın sonunda istatistiksel olarak bir azalma gözlendi. Anormal sperm morfolojisi bakımından kontrol grubu ratlarla dichlorvos, vitamin C ve E+dichlorvos grubu ratlar karsılastırıldıgında 4. ve 7. haftanın sonunda istatistiksel olarak bir artıs gözlendi. FSH, LH ve testosteron düzeyleri 4. ve 7. haftanın sonunda kontrol grubu ile karsılastırıldıgında istatistiksel olarak bir azalma tespit edildi. Bununla beraber, testosteron düzeyleri bakımından vitamin C+vitamin E+dichlorvos muameleli grup, dichlorvos grubu ratlarla karsılastırıldıgında 4. ve 7. haftanın sonunda istatistiksel olarak bir artıs gözlendi. Isık ve elektron mikroskobu ile yapılan incelemelerde dichlorvos muameleli ratlarla, vitamin C+vitamin E+dichlorvos muameleli ratların seminifer tüpçüklerinde 4. ve 7. haftanın sonunda patolojik degisiklikler gözlendi. Sonuç olarak dichlorvos'un ratlarda sebep oldugu testiküler toksisiteyi vitamin C ve vitamin E'nin önlemedigi tespit edildi.Dichlorvos is an organophosphate insecticide, which is widely used in pest control. In this study, male rats were divided into four groups. Control group, vitamin C+vitamin E-treatment (200 mg/kg bw per day) group, dichlorvos treatment group (1,6 mg/kg bw per day) and vitamin C+vitamin E (200 mg/kg bw per day)+dichlorvos treatment (1,6 mg/kg bw per day) group. All combinations were given to rats orally via gavage for 7 weeks. At the end of the 24 hrs, 4th and 7th weeks, body and testis weights of control and treated rats were measured. Testicular sperm count, epididymal sperm motility, morphology and serum follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone levels were determined and pathological changes of the testicular tissues were evaluated by light and electron microscopy. Evaluation of sperm concentration after 24 hrs, 4th and 7th weeks did not show any statistically significant difference when control group was compared to dichlorvos and vitamin C and E+dichlorvos treated group. After 7 weeks of dichlorvos administration, a decrease of epididymal sperm motility occurred in male rats. Increase of abnormal sperm count occurred 4 and 7 weeks after dichlorvos treatment. In the present study, FSH, LH and testosterone levels were decreased statistically after 4 and 7 weeks of exposure when control group was compared to dichlorvos and vitamin C and E+dichlorvos group. Apart, when control group was compared to vitamin C and E treated group, FSH and LH levels were not altered. Vitamins E and C administration resulted in a statistically significant increase in plasma testosterone levels when vitamin C and E+dichlorvos group was compared to dichlorvos group. Light and electron microscopic investigations figured out pathological changes in dichlorvos and vitamin C and E+dichlorvos treated groups after 4 and 7 weeks of exposure. As a result, vitamins C and E were not able to recover the testicular toxicity of dichlorvos in rats

Association of serum and follicular fluid leptin and ghrelin levels with in vitro fertilization success

Dirican, Enver/0000-0001-6230-1229; Gode, Funda/0000-0002-9371-6942; Dirican, Enver/0000-0001-6230-1229WOS: 000411999200002PubMed: 29949336Objectives: The aim of this study was to evaluate the relationship between in vitro fertilization (IVF) cycle outcomes, serum and follicular fluid (FF) levels of leptin and ghrelin. Material and methods: Forty-four women who underwent intracytoplasmic sperm injection cycles (ICSI) were enrolled in the study. On the third day (D3) of the menstrual cycle, venous blood samples were drawn for serum measurements of leptin and ghrelin. The follicular fluid (FF) and the corresponding oocyte were obtained from a single dominant preovulatory follicle at the time of oocyte pick-up. The FF and D3 serum leptin and ghrelin concentrations were measured by enzyme-linked immunosorbent assay. The relationship between pregnancy rate and serum, follicular fluid levels of leptin and ghrelin were analyzed. Results: Of the 44 cases included, nineteen achieved clinical pregnancy (43.18%). Follicular fluid ghrelin levels were significantly lower in the pregnant group than non-pregnant group (p 0.05). There was no statistically significant difference in D3 serum ghrelin between pregnant and non-pregnant groups (p > 0.05). However, D3 serum leptin levels were significantly lower in pregnant women than non-pregnant women (p < 0.05). Conclusions: Lower ghrelin levels in the follicular fluid were associated with higher pregnancy rates. Also, D3 serum leptin levels were inversely correlated with clinical pregnancy rates. These findings support the potential role of these molecules on IVF outcomes

Association of serum and follicular fluid leptin and ghrelin levels with in vitro fertilization success

Objectives: The aim of this study was to evaluate the relationship between in vitro fertilization (IVF) cycle outcomes, serum and follicular fluid (FF) levels of leptin and ghrelin

Association of serum and follicular fluid leptin and ghrelin levels with in vitro fertilization success

Objectives: The aim of this study was to evaluate the relationship between in vitro fertilization (IVF) cycle outcomes, serum and follicular fluid (FF) levels of leptin and ghrelin