Small and large scale genomic DNA isolation protocol for chickpea (Cicer arietinum L.), suitable for molecular marker and transgenic analyses

Chickpea is an important food legume crop with high nutritional value. Lack of appropriate DNA isolation protocol is a limiting factor for any molecular studies of this crop. The present report describes a rapid and efficient protocol for small and large scale preparation of superior quality and quantity of DNA from four cultivars (JG62, WR315, C235 and ICCV89314) compared to that of earlier reports. The yield of DNA through both the methods was estimated to be approximately 80 μg per g of plant tissue. Both small and large scale preparations were essentially suitable for PCR and Southern blot hybridization analyses, which are the key steps in crop improvement programme through marker development and genetic engineering techniques.Key words: Cicer arietinum L., phenolics, restriction enzyme digestion, PCR amplification, Southern hybridization

New physics implications of VBF searches exemplified through the Georgi-Machacek model

LHC searches for nonstandard scalars in vector boson fusion (VBF) production processes can be particularly efficient in probing scalars belonging to triplet or higher multiplet representations of the Standard Model $SU(2)_L$ gauge group. They can be especially relevant for models where the additional scalars do not have any tree-level couplings to the Standard Model fermions, rendering VBF as their primary production mode at the LHC. In this work, we employ the latest LHC data from VBF resonance searches to constrain the properties of nonstandard scalars, taking the Georgi-Machacek model as a prototypical example. We take into account the theoretical constraints on the potential from unitarity and boundedness-from-below as well as indirect constraints coming from the signal strength measurements of the 125 GeV Higgs boson at the LHC. To facilitate the phenomenological analysis we advocate a convenient reparametrization of the trilinear couplings in the scalar potential. We derive simple correlations among the model parameters corresponding to the decoupling limit of the model. We explicitly demonstrate how a combination of theoretical and phenomenological constraints can push the GM model towards the decoupling limit. Our analysis suggests that the VBF searches can provide key insights into the composition of the electroweak vacuum expectation value.Comment: 17 pages, 7 figure

Primary Metabolism of Chickpea Is the Initial Target of Wound Inducing Early Sensed Fusarium oxysporum f. sp. ciceri Race I

BACKGROUND: Biotrophic interaction between host and pathogen induces generation of reactive oxygen species that leads to programmed cell death of the host tissue specifically encompassing the site of infection conferring resistance to the host. However, in the present study, biotrophic relationship between Fusarium oxysporum and chickpea provided some novel insights into the classical concepts of defense signaling and disease perception where ROS (reactive oxygen species) generation followed by hypersensitive responses determined the magnitude of susceptibility or resistant potentiality of the host. METHODOLOGY/PRINCIPAL FINDINGS: Microscopic observations detected wound mediated in planta pathogenic establishment and its gradual progression within the host vascular tissue. cDNA-AFLP showed differential expression of many defense responsive elements. Real time expression profiling also validated the early recognition of the wound inducing pathogen by the host. The interplay between fungus and host activated changes in primary metabolism, which generated defense signals in the form of sugar molecules for combating pathogenic encounter. CONCLUSIONS/SIGNIFICANCE: The present study showed the limitations of hypersensitive response mediated resistance, especially when foreign encounters involved the food production as well as the translocation machinery of the host. It was also predicted from the obtained results that hypersensitivity and active species generation failed to impart host defense in compatible interaction between chickpea and Fusarium. On the contrary, the defense related gene(s) played a critical role in conferring natural resistance to the resistant host. Thus, this study suggests that natural selection is the decisive factor for selecting and segregating out the suitable type of defense mechanism to be undertaken by the host without disturbing its normal metabolism, which could deviate from the known classical defense mechanisms

Allergenicity Assessment of Allium sativum Leaf Agglutinin, a Potential Candidate Protein for Developing Sap Sucking Insect Resistant Food Crops

Background: Mannose-binding Allium sativum leaf agglutinin (ASAL) is highly antinutritional and toxic to various phloem-feeding hemipteran insects. ASAL has been expressed in a number of agriculturally important crops to develop resistance against those insects. Awareness of the safety aspect of ASAL is absolutely essential for developing ASAL transgenic plants. Methodology/Principal Findings: Following the guidelines framed by the Food and Agriculture Organization/World Health Organization, the source of the gene, its sequence homology with potent allergens, clinical tests on mammalian systems, and the pepsin resistance and thermostability of the protein were considered to address the issue. No significant homology to the ASAL sequence was detected when compared to known allergenic proteins. The ELISA of blood sera collected from known allergy patients also failed to show significant evidence of cross-reactivity. In vitro and in vivo assays both indicated the digestibility of ASAL in the presence of pepsin in a minimum time period. Conclusions/Significance: With these experiments, we concluded that ASAL does not possess any apparent features of an allergen. This is the first report regarding the monitoring of the allergenicity of any mannose-binding monocot lectin having insecticidal efficacy against hemipteran insects

Arsenic Contamination in Groundwater: A Global Perspective with Emphasis on the Asian Scenario

The incidence of high concentrations of arsenic in drinking-water has emerged as a major public-health problem. With newer-affected sites discovered during the last decade, a significant change has been observed in the global scenario of arsenic contamination, especially in Asian countries. This communication presents an overview of the current scenario of arsenic contamination in countries across the globe with an emphasis on Asia. Along with the present situation in severely-affected countries in Asia, such as Bangladesh, India, and China, recent instances from Pakistan, Myanmar, Afghanistan, Cambodia, etc. are presented

Arsenic exposure and outcomes of antimonial treatment in visceral leishmaniasis patients in bihar, India:a retrospective cohort study

Funding: This work was supported by a Clinical PhD Fellowship to MRP (090665) and a Principal Research Fellowship to AHF (079838) from the Wellcome Trust (http://www.wellcome.ac.uk). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD

An Eight-year Study Report on Arsenic Contamination in Groundwater and Health Effects in Eruani Village, Bangladesh and an Approach for Its Mitigation

Based on several surveys during 1997-2005 and visits of a medical team to Eruani village, Laksham upazila, Comilla district, Bangladesh, the arsenic contamination situation and consequent clinical manifestations of arsenicosis among the villagers, including dermatology, neuropathy, and obstetric outcome, are reported here. Analysis of biological samples from patients and non-patients showed high body burden of arsenic. Even after eight years of known exposure, village children were still drinking arsenic-contaminated water, and many of them had arsenical skin lesions. There were social problems due to the symptoms of arsenicosis. The last survey established that there is a lack of proper awareness among villagers about different aspects of arsenic toxicity. The viability of different options of safe water, such as dugwells, deep tubewells, rainwater harvesting, and surface water with watershed management in the village, was studied. Finally, based on 19 years of field experience, it was felt that, for any successful mitigation programme, emphasis should be given to creating awareness among villagers about the arsenic problem, role of arsenic-free water, better nutrition from local fruits and vegetables, and, above all, active participation of women along with others in the struggle against the arsenic menace