Guidelines for assessing favourable conservation status of Natura 2000 species and habitat types in Bulgaria

This executive summary describes the methodology for assessing the favourable conservation status of N2000 habitats and species on site level in Bulgaria and gives guidelines for its application. The methodology was developed in the frame of the BBI/Matra project 2006/014 “Favourable Conservation Status of Natura 2000 Habitat types and Species in Bulgaria”. The project was generously supported by the Dutch government under the BBI/Matra programme, which is a combination of two international policy programs of the Dutch government. The objectives and financial resources of the BBI/Matra Programme fall within the remit of the Matra Social Transformation Program of the Ministry of Foreign Affairs and under the International Policy Program on Biodiversity of the Ministry of Agriculture, Nature and Food Quality

Optimized production of large Bose Einstein Condensates

We suggest different simple schemes to efficiently load and evaporate a ''dimple'' crossed dipolar trap. The collisional processes between atoms which are trapped in a reservoir load in a non adiabatic way the dimple. The reservoir trap can be provided either by a dark SPOT Magneto Optical Trap, the (aberrated) laser beam itself or by a quadrupolar or quadratic magnetic trap. Optimal parameters for the dimple are derived from thermodynamical equations and from loading time, including possible inelastic and Majorana losses. We suggest to load at relatively high temperature a tight optical trap. Simple evaporative cooling equations, taking into account gravity, the possible occurrence of hydrodynamical regime, Feshbach resonance processes and three body recombination events are given. To have an efficient evaporation the elastic collisional rate (in s$^{-1}$) is found to be on the order of the trapping frequency and lower than one hundred times the temperature in micro-Kelvin. Bose Einstein condensates with more than $10^7$ atoms should be obtained in much less than one second starting from an usual MOT setup.Comment: 14 page

A vesicle microrheometer for high-throughput viscosity measurements of lipid and polymer membranes

Viscosity is a key property of cell membranes that controls mobility of embedded proteins and membrane remodeling. Measuring it is challenging because existing approaches involve complex experimental designs and/or models, and the applicability of some is limited to specific systems and membrane compositions. As a result there is scarcity of systematic data and the reported values for membrane viscosity vary by orders of magnitude for the same system. Here, we show how viscosity of bilayer membranes can be easily obtained from the transient deformation of giant unilamellar vesicles. The approach enables a non-invasive, probe-independent and high-throughput measurement of the viscosity of bilayers made of lipids or polymers with a wide range of compositions and phase state. Using this novel method, we have collected a significant amount of data that provide insights into the relation between membrane viscosity, composition, and structure

Greenhouse gas balance over thaw-freeze cycles in discontinuous zone permafrost

Peat in the discontinuous permafrost zone contains a globally significant reservoir of carbon that has undergone multiple permafrost-thaw cycles since the end of the mid-Holocene (~3700 years before present). Periods of thaw increase C decomposition rates which leads to the release of CO2 and CH4 to the atmosphere creating potential climate feedback. To determine the magnitude and direction of such feedback, we measured CO2 and CH4 emissions and modeled C accumulation rates and radiative fluxes from measurements of two radioactive tracers with differing lifetimes to describe the C balance of the peatland over multiple permafrost-thaw cycles since the initiation of permafrost at the site. At thaw features, the balance between increased primary production and higher CH4 emission stimulated by warmer temperatures and wetter conditions favors C sequestration and enhanced peat accumulation. Flux measurements suggest that frozen plateaus may intermittently (order of years to decades) act as CO2 sources depending on temperature and net ecosystem respiration rates, but modeling results suggest that—despite brief periods of net C loss to the atmosphere at the initiation of thaw—integrated over millennia, these sites have acted as net C sinks via peat accumulation. In greenhouse gas terms, the transition from frozen permafrost to thawed wetland is accompanied by increasing CO2 uptake that is partially offset by increasing CH4 emissions. In the short-term (decadal time scale) the net effect of this transition is likely enhanced warming via increased radiative C emissions, while in the long-term (centuries) net C deposition provides a negative feedback to climate warming

Skeletal Muscle Gender Dimorphism from Proteomics

Gross contraction in skeletal muscle is primarily determined by a relatively small number of contractile proteins, however this tissue is also remarkably adaptable to environmental factors1 such as hypertrophy by resistance exercise and atrophy by disuse. It thereby exhibits remodeling and adaptations to stressors (heat, ischemia, heavy metals, etc.)2,3. Damage can occur to muscle by a muscle exerting force while lengthening, the so-called eccentric contraction4. The contractile proteins can be damaged in such exertions and need to be repaired, degraded and/or resynthesized; these functions are not part of the contractile proteins, but of other much less abundant proteins in the cell. To determine what subset of proteins is involved in the amelioration of this type of damage, a global proteome must be established prior to exercise5 and then followed subsequent to the exercise to determine the differential protein expression and thereby highlight candidate proteins in the adaptations to damage and its repair. Furthermore, most studies of skeletal muscle have been conducted on the male of the species and hence may not be representative of female muscle

STUDIES ON CYTOMEGALOVIRAL (CMV) INFECTIONS IN NEWBORN CHILDREN

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