Efeito do probiótico sobre o desempenho, rendimento de carcaça e exigências nutricionais de proteína bruta de codornas de corte Effect of the addition of probiotic on performance, carcass yield and crude protein nutritional requirements of meat quails

Para avaliar o efeito do probiótico (Lactobacillus sp.) associado a diferentes níveis de proteína bruta (PB) sobre o desempenho, as exigências nutricionais de PB, o rendimento de carcaça e de cortes de codornas de corte, foram utilizadas 2.304 codornas de corte, distribuídas em esquema fatorial 2 × 4 (com e sem probiótico e 15, 20, 25 ou 30% de PB), com duas repetições por nível de PB, repetido duas vezes no tempo (dois períodos experimentais), totalizando 32 unidades experimentais. A adição do probiótico reduziu o consumo de ração e melhorou a conversão alimentar no período de 1 a 7 dias de idade. De acordo com os pesos médios do modelo Linear Response Plateau (LRP), aos 7 e aos 14 dias, as estimativas de exigência foram de 27% de PB e aos 21, 28 e 35 dias, 22 a 24% de PB. A adição do probiótico não influenciou o rendimento de carcaça e de cortes. Para o rendimento de carcaça, a exigência de PB, de acordo com o modelo quadrático, foi estimada em 23,53%, mas, pelo modelo LRP, a estimativa reduziu para 17,13% de PB. Nas condições ambientais em que as codornas foram criadas, a adição do probiótico não influenciou o desempenho, as exigências nutricionais de PB e o rendimento de carcaça de codornas de corte. As exigências de PB para aves de até 14 dias de idade são de 27% e, após esta idade, reduzem para 22 a 24%.<br>To evaluate the effect of probiotic (Lactobacillus sp.) associated with different crude protein (CP) levels on performance, CP nutritional requirements, carcass and cut yields of meat quails, a total of 2304 meat quails were distributed in a 2 × 4 factorial design (with and without probiotic and four CP levels - 15, 20, 25 and 30%), with two replications per treatment, repeated twice in time (two experimental periods), totalizing 32 experimental units. The addition of probiotic decreased the feed intake and improved the feed conversion rate at 1 to 7 days of age. According to the mean weight of the LRP model, at 7 and 14 days, the requirement estimations were of 27% of CP and at 21, 28 and 35 days, the requirements were from 22 to 24% of CP. The addition of probiotic did not influence carcass and cut yields. For the carcass yield, the CP requirement, according to the quadratic model, was estimated in 23.53%; however, in relation to LRP, the estimative decrease to 17.13% of CP. It was possible to conclude that in the environmental conditions evaluated, the addition of probiotic did not influence the performance, CP nutritional requirement and carcass yield of meat quails. The CP requirements for birds of up to 14 days of age were of 27% and, after this age, there are evidences of reduction to values from 22 to 24%

Mechanisms of cell transformation induced by polyomavirus

Polyomavirus is a DNA tumor virus that induces a variety of tumors in mice. Its genome encodes three proteins, namely large T (LT), middle T (MT), and small T (ST) antigens, that have been implicated in cell transformation and tumorigenesis. LT is associated with cell immortalization, whereas MT plays an essential role in cell transformation by binding to and activating several cytoplasmic proteins that participate in growth factor-induced mitogenic signal transduction to the nucleus. The use of different MT mutants has led to the identification of MT-binding proteins as well as analysis of their importance during cell transformation. Studying the molecular mechanisms of cell transformation by MT has contributed to a better understanding of cell cycle regulation and growth control

Perspectives on ethnic and racial disparities in Alzheimer&apos;s disease and related dementias: Update and areas of immediate need

Alzheimer&apos;s disease and related dementias (ADRDs) are a global crisis facing the aging population and society as a whole. With the numbers of people with ADRDs predicted to rise dramatically across the world, the scientific community can no longer neglect the need for research focusing on ADRDs among underrepresented ethnoracial diverse groups. The Alzheimer&apos;s Association International Society to Advance Alzheimer&apos;s Research and Treatment (ISTAART; alz.org/ISTAART) comprises a number of professional interest areas (PIAs), each focusing on a major scientific area associated with ADRDs. We leverage the expertise of the existing international cadre of ISTAART scientists and experts to synthesize a cross-PIA white paper that provides both a concise “state-of-the-science” report of ethnoracial factors across PIA foci and updated recommendations to address immediate needs to advance ADRD science across ethnoracial populations. © 2018 The Author

The Drosophila Nuclear Lamina Protein YA Binds to DNA and Histone H2B with Four Domains

Dramatic changes occur in nuclear organization and function during the critical developmental transition from meiosis to mitosis. The Drosophila nuclear lamina protein YA binds to chromatin and is uniquely required for this transition. In this study, we dissected YA's binding to chromatin. We found that YA can bind to chromatin directly and specifically. It binds to DNA but not RNA, with a preference for double-stranded DNA (linear or supercoiled) over single-stranded DNA. It also binds to histone H2B. YA's binding to DNA and histone H2B is mediated by four domains distributed along the length of the YA molecule. A model for YA function at the end of Drosophila female meiosis is proposed

Nup98 is a mobile nucleoporin with transcription-dependent dynamics

Nucleoporin 98 (Nup98), a glycine-leucine-phenylalanine-glycine (GLFG) amino acid repeat-containing nucleoporin, plays a critical part in nuclear trafficking. Injection of antibodies to Nup98 into the nucleus blocks the export of most RNAs. Nup98 contains binding sites for several transport factors; however, the mechanism by which this nucleoporin functions has remained unclear. Multiple subcellular localizations have been suggested for Nup98. Here we show that Nup98 is indeed found both at the nuclear pore complex and within the nucleus. Inside the nucleus, Nup98 associates with a novel nuclear structure that we term the GLFG body because the GLFG domain of Nup98 is required for targeting to this structure. Photobleaching of green fluorescent protein-Nup98 in living cells reveals that Nup98 is mobile and moves between these different localizations. The rate of recovery after photobleaching indicates that Nup98 interacts with other, less mobile, components in the nucleoplasm. Strikingly, given the previous link to nuclear export, the mobility of Nup98 within the nucleus and at the pore is dependent on ongoing transcription by RNA polymerases I and II. These data give rise to a model in which Nup98 aids in direction of RNAs to the nuclear pore and provide the first potential mechanism for the role of a mobile nucleoporin