13 research outputs found
Antimicrobial resistance levels amongst staphylococci isolated from clinical cases of bovine mastitis in Kosovo
Introduction: Mastitis is one of the most frequent and costly disease in cattle. We studied milk samples from cattle with mastitis from farms in Kosovo to identify mastitis-causing pathogens and possible effective antibiotics. Our ultimate goal is to help implement adequate antibiotic management and treatment practices in Kosovo Methodology: A total of 152 milk samples were collected from cows with clinical mastitis from different farms in Kosovo. After identification of microorganisms, antibiotic susceptibility and the occurrence of enterotoxins was investigated. Results: Staphylococci were found in 89 samples, of which 58 were coagulase negative and 31 coagulase positive. S. aureus was isolated from 27 samples, S. epidermidis from 25, and S. chromogenes from 15, while other species of staphylococci were isolated from the remaining 22 isolates. Interestingly, the bacterial diversity was different between cows in different periods of lactation and among different breeds. Most of the isolates (76/89) were resistant to two or more antibiotics. The highest resistance was to penicillin and ampicillin (> 65%), followed by tetracycline, oxacillin, streptomycin, chloramphenicol (> 23%), and less than 3% to erythromycin. Of the 89 isolates, 40 produced enterotoxins that were most frequently typed as A and C. Conclusions: We detected human bacterial pathogens in the cultures of milk samples from cows with mastitis. The isolates demonstrated resistance to two or more antibiotics, some of which are frequently used to treat animal and human infections. We recommend increased control and more stringent use of antibiotics in veterinary as well as human medicine
Bioluminescence based biosensors for quantitative detection of enterococcal peptide - Pheromone activity reveal inter-strain telesensing in vivo during polymicrobial systemic infection
The interaction between Staphylococcus aureus SdrD and desmoglein 1 is important for adhesion to host cells
Efficient inactivation of multi-antibiotics resistant nosocomial Enterococci by purified Hiracin Bacteriocin
The interaction between Staphylococcus aureus SdrD and desmoglein 1 is important for adhesion to host cells
Staphylococcus aureus is known as a frequent colonizer of the skin and mucosa. Among bacterial factors
involved in colonization are adhesins such as the microbial surface components recognizing adhesive
matrix molecules (MSCRAMMs). Serine aspartate repeat containing protein D (SdrD) is involved in
adhesion to human squamous cells isolated from the nose. Here, we identify Desmoglein 1 (Dsg1)
as a novel interaction partner for SdrD. Genetic deletion of
sdrD in S. aureusNCTC8325-4 through
allelic replacement resulted in decreased bacterial adherence to Dsg1- expressing HaCaT cells
in vitro.
Complementary gain-of-function was demonstrated by heterologous expression of SdrD in Lactococcus
lactis, which increased adherence to HaCaT cells. Also ectopic expression of Dsg1 in HEK293 cells
resulted in increased adherence of S. aureus
NCTC8325-4 in vitro. Increased adherence of NCTC8325-4, compared to NCTC8325-4ΔsdrD,
to the recombinant immobilized Dsg1 demonstrated direct interaction between SdrD and Dsg1. Specificity of SdrD interaction with Dsg1 was further verified using flow
cytometry and confirmed binding of recombinant SdrD to HaCaT cells expressing Dsg1 on their surface.
These data demonstrate that Dsg1 is a host ligand for SdrD