RNAseq reveals hydrophobins that are involved in the adaptation of aspergillus nidulans to lignocellulose

Background Sugarcane is one of the world’s most profitable crops. Waste steam-exploded sugarcane bagasse (SEB) is a cheap, abundant, and renewable lignocellulosic feedstock for the next-generation biofuels. In nature, fungi seldom exist as planktonic cells, similar to those found in the nutrient-rich environment created within an industrial fermenter. Instead, fungi predominantly form biofilms that allow them to thrive in hostile environments. Results In turn, we adopted an RNA-sequencing approach to interrogate how the model fungus, Aspergillus nidulans, adapts to SEB, revealing the induction of carbon starvation responses and the lignocellulolytic machinery, in addition to morphological adaptations. Genetic analyses showed the importance of hydrophobins for growth on SEB. The major hydrophobin, RodA, was retained within the fungal biofilm on SEB fibres. The StuA transcription factor that regulates fungal morphology was up-regulated during growth on SEB and controlled hydrophobin gene induction. The absence of the RodA or DewC hydrophobins reduced biofilm formation. The loss of a RodA or a functional StuA reduced the retention of the hydrolytic enzymes within the vicinity of the fungus. Hence, hydrophobins promote biofilm formation on SEB, and may enhance lignocellulose utilisation via promoting a compact substrate-enzyme-fungus structure. Conclusion This novel study highlights the importance of hydrophobins to the formation of biofilms and the efficient deconstruction of lignocellulose

Ultrafast dynamics of au nanopyramid interfaces prepared by nanosphere lithography: effect of substrate chemical composition

This work describes ultrafast spectroscopy studies of Au triangular pyramid particle arrays deposited over glass (termed Au/glass), and 190 nm indium tin oxide (ITO) film (termed Au/ITO/glass) prepared by nanosphere lithography. The linear absorption spectra of Au/glass and Au/ITO/glass exhibit surface plasmon resonances at 800 and 870 nm, respectively, in good agreement with discrete dipole approximation simulations. Ultrafast pump-probe measurements at wavelengths below resonance, at resonance, and above the surface plasmon resonance for each of these two systems are presented. The pump-probe measurements on both systems can be well fit with a model accounting for electron-electron scattering, electron-phonon coupling, and acoustic oscillations on top of cooling of the gold lattice. Numerical simulations employing a two-temperature model are consistent with the single-color pump-probe exponential decays. The wavelength-dependent pump-probe results are interpreted in terms of the complex wavelength-dependent refractive index of gold. We show that this interpretation is consistent with diffractive-optic four-wave mixing spectroscopy measurements of absorptive and dispersive parts of the third-order nonlinear polarization at 800 nm272423433FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPSem informaçã

Aspectos da cultura do estado de Goiás

ResumoO conceito de cultura abrange vários e diferentes aspectos. A respeito da cultura goiana, a raiz atrelada ao campo traduz o modo de falar, se portar e se vestir, além de repercutir nas tradições, festas, rituais, símbolos – compondo a sua memória e identidade. Porém, devido à modernização dos meios de produção e do modo de vida caipira, a raiz da identidade cultural goiana corre o risco de desaparecer sem ter sido amplamente conhecido pela comunidade. Nesse sentido, tem-se por objetivo apresentar um esboço descritivo a respeito dos principais aspectos da cultura do estado de Goiás. Para desenvolvimento do trabalho, foi utilizada a técnica documentação indireta referente à pesquisa documental e bibliográfica. Os aspectos culturais do Estado de Goiás são bem diversificados, representados por diversas por modalidades e vários seguimentos, como a música, as artes plásticas, as danças, as festas, a gastronomia, as lendas e o próprio modo de se comunicar (a fala). Estes aspectos compõem a estruturação e formação cultural de um Estado.Palavras-chave: Cultura, Goiás, Aspectos, Culturais, Geografia. AbstractThe concept of culture encompasses many different aspects. Regarding the culture of Goiás, the root linked to the field translates the way of speaking, behaving and dressing, as well as reverberating in traditions, festivals, rituals, symbols - composing their memory and identity. However, due to the modernization of the means of production and the country way of life, the root of the cultural identity of Goiás is in danger of disappearing without being widely known by the community. In this sense, this work aim to present a descriptive sketch about the main aspects of the culture of the state of Goiás. For the development of the work, the technique indirect documentation related to documentary and bibliographic research was used. The cultural aspects of the State of Goiás are well diversified, represented by diverse forms and various followings, such as music, plastic arts, dances, festivals, gastronomy, legends and the way of communicating itself (speech). These aspects make up the structuring and cultural formation of a State.Key-words: Culture, Goias, Aspects, Cultural, Geography

Fatores de risco cardiovascular e distribuição de gordura corporal em brasileiros com 80 anos ou mais

Modelo do estudo: Estudo transversal. Objetivo: Analisar a presença de fatores de risco cardiovascular (FRC) de acordo com o padrão de distribuição de gordura corporal em brasileiros com 80 anos ou mais. Materiais e Método: A amostra foi composta de 113 idosos, com média de idade de 83,4 anos, de ambos os sexos. O percentual de gordura corporal total e abdominal, hipertensão e perfil lipídico foram usados para caracterização dos FRC. O teste qui-quadrado foi utilizado para avaliação das proporções de FRC e o teste Mann-Whitney foi utilizado para comparar os resultados de adiposidade. Resultados: Idosos eutróficos apresentaram baixo triglicerídeos (p=0,017) e colesterol total (p=0,001) além de menores prevalências de hipertensão (p=0,003) e hipertrigliceridemia (p=0,007). Idosos com maior adiposidade abdominal apresentaram maior colesterol total (p=0,026) e prevalência de hipertrigliceridemia (p=0,011) e hipercolesterolemia (p=0,026) quando comparados com os de menor adiposidade abdominal. Aqueles com excesso de gordura (total, ou abdominal ou ambos), com exceção da glicemia (p=0,877), apresentaram alta prevalência de FRC. Aqueles com ambos os excessos de gordura apresentam diferenças, com maior nível de prevalência quando comparados aqueles com apenas um ou nenhum dos excessos. Conclusão: A obesidade, sendo abdominal ou total, é associada, da mesma maneira, com FRC em sujeitos com 80 anos ou maisStudy design: Cross-sectional study. Objective: To analyze the presence of cardiovascular risk factors (CRFs) according to the pattern of body fat distribution, in Brazilians aged 80 years or over. Methods: The sample consisted of 113 subjects, of 83.4 years, of both sexes. The percentages of total and abdominal body fat, hypertension and lipid profile were used for characterization of CRFs. The chisquare test was used to assess proportions of CRFs and the Mann-Whitney test was used to compare the results of adiposity. Results: Eutrophic subjects presented lower triglycerides (p=0.017), total cholesterol (p=0.001) and prevalence of both hypertension (p=0.003) and hypertriglyceridemia (p=0.007). Subjects with higher abdominal adiposity presented higher total cholesterol (p=0.026) and prevalence of hypertriglyceridemia (p=0.011) and hypercholesterolemia (p=0.026) than those with no excess abdominal adiposity. Those with excess fat (total, abdominal or both), with the exception of glucose (p=0.877), presented a high prevalence of CRFs. Those with both types of excess fat presented differences, with a higher prevalence level when compared to those with only one or neither of the excesses. Conclusion: Obesity, whether abdominal or total, is similarly associated with CRFs in subjects aged 80 years and ove

Association between body composition, balance and mobility of elderly aged 80 years or older

Modelo do estudo: Estudo transversal. Objetivo: O objetivo do presente estudo foi analisar a associação entre composição corporal, mobilidade e equilíbrio de idosos com idade igual ou superior a 80 anos. Métodos: A amostra foi composta por 123 idosos com idade entre 80 e 95 anos (83,2±2,7 anos), sendo 78 mulheres (83,2±2,9 anos) e 45 homens (83,2±2,4 anos) residentes na área urbana de Presidente Prudente - SP. A avaliação da composição corporal foi feita pelo método de Absorciometria por Dupla Emissão de Raios-X (DEXA). A mobilidade e o equilíbrio foram avaliados por meio dos testes de velocidade para caminhar, equilíbrio estático e força de membros inferiores. Para tratamento estatístico realizou-se o teste qui-quadrado, o software utilizado foi SPSS (13.0) e o nível de significância estabelecido foi de 5%. Resultados: No grupo masculino, os idosos com maior desempenho nos testes funcionais apresentaram maiores valores percentuais de massa muscular (MM) (35,6%) comparados aos de menor desempenho (15,6%), p=0,026. No grupo feminino, as idosas com maior desempenho nos testes apresentaram maiores valores de densidade mineral óssea (DMO) (30,8%) comparadas as de menor desempenho (20,5%) p=0,041. Conclusão: A MM para idosos do sexo masculino e a DMO para o sexo feminino foram os componentes da composição corporal que se associaram com a capacidade funcional.Study design: cross-sectional study. Objective: The objective of this study was to analyze the association between body composition, mobility and balance of elderly aged 80 years or older. Methods: The sample consisted of 123 elderly aged 80 and 95 years (83.2 ± 2.7 years) with 78 women (83.2 ± 2.9 years) and 45 men (83.2 ± 2, 4 years) residing in the city of Presidente Prudente - SP. Assessment of body composition was made by absorpiometria dual energy X-ray (DXA). The mobility and balance were assessed by means of tests of speed walking, and static balance lower limb strength. For statistical analysis we carried out the chi-square test, the software used was SPSS (13.0) and the significance level was set at 5%. Results: In males, those with higher performance in the functional tests showed higher percentages of muscle mass (MM) (35.6%) compared to lower performance (15.6%), p = 0.026. In the female group,the elderly with higher performance on tests showed higher bone mineral density (BMD) values (30.8%)compared with those with lower performance (20.5%) p = 0.041. Conclusion: MM for elderly males and BMD for females were the components of body composition that is associated with functional capacity

Critical assessment of enhancement factor measurements in surface-enhanced raman scattering on different substrates

The SERS enhancement factor (SERS-EF) is one of the most important parameters that characterizes the ability of a given substrate to enhance the Raman signal for SERS applications. The comparison of SERS intensities and SERS-EF values across different substrates is a common practice to unravel the performance of a given substrate. In this study, it is shown that such a comparison may lack significance if we compare substrates of very distinct nature and optical properties. It is specifically shown that the SERS-EF values for static substrates (e.g. immobilized metallic nanostructures) cannot be compared to those of dynamic ones (e.g. colloidal metal nanoparticle solutions), and that the optical properties for the latter show strong dependence on the metal-molecule interaction dynamics. The most representative experimental results concerning the dynamic substrates have been supported by generalized Mie theory simulations, which are tools used to describe the substrate complexity and the microscopic information not usually taken into account17332129421301CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE MINAS GERAIS - FAPEMIGFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPES

Predictive Maintenance Model Based on Anomaly Detection in Induction Motors: A Machine Learning Approach Using Real-Time IoT Data

With the support of Internet of Things (IoT) devices, it is possible to acquire data from degradation phenomena and design data-driven models to perform anomaly detection in industrial equipment. This approach not only identifies potential anomalies but can also serve as a first step toward building predictive maintenance policies. In this work, we demonstrate a novel anomaly detection system on induction motors used in pumps, compressors, fans, and other industrial machines. This work evaluates a combination of pre-processing techniques and machine learning (ML) models with a low computational cost. We use a combination of pre-processing techniques such as Fast Fourier Transform (FFT), Wavelet Transform (WT), and binning, which are well-known approaches for extracting features from raw data. We also aim to guarantee an optimal balance between multiple conflicting parameters, such as anomaly detection rate, false positive rate, and inference speed of the solution. To this end, multiobjective optimization and analysis are performed on the evaluated models. Pareto-optimal solutions are presented to select which models have the best results regarding classification metrics and computational effort. Differently from most works in this field that use publicly available datasets to validate their models, we propose an end-to-end solution combining low-cost and readily available IoT sensors. The approach is validated by acquiring a custom dataset from induction motors. Also, we fuse vibration, temperature, and noise data from these sensors as the input to the proposed ML model. Therefore, we aim to propose a methodology general enough to be applied in different industrial contexts in the future

Anomaly Detection in Industrial Machinery using IoT Devices and Machine Learning: a Systematic Mapping

Anomaly detection is critical in the smart industry for preventing equipment failure, reducing downtime, and improving safety. Internet of Things (IoT) has enabled the collection of large volumes of data from industrial machinery, providing a rich source of information for Anomaly Detection. However, the volume and complexity of data generated by the Internet of Things ecosystems make it difficult for humans to detect anomalies manually. Machine learning (ML) algorithms can automate anomaly detection in industrial machinery by analyzing generated data. Besides, each technique has specific strengths and weaknesses based on the data nature and its corresponding systems. However, the current systematic mapping studies on Anomaly Detection primarily focus on addressing network and cybersecurity-related problems, with limited attention given to the industrial sector. Additionally, these studies do not cover the challenges involved in using ML for Anomaly Detection in industrial machinery within the context of the IoT ecosystems. This paper presents a systematic mapping study on Anomaly Detection for industrial machinery using IoT devices and ML algorithms to address this gap. The study comprehensively evaluates 84 relevant studies spanning from 2016 to 2023, providing an extensive review of Anomaly Detection research. Our findings identify the most commonly used algorithms, preprocessing techniques, and sensor types. Additionally, this review identifies application areas and points to future challenges and research opportunities

Periostin as a modulator of chronic cardiac remodeling after myocardial infarction

OBJECTIVE: After acute myocardial infarction, during the cardiac repair phase, periostin is released into the infarct and activates signaling pathways that are essential for the reparative process. However, the role of periostin in chronic cardiac remodeling after myocardial infarction remains to be elucidated. Therefore, the objective of this study was to investigate the relationship between tissue periostin and cardiac variables in the chronic cardiac remodeling induced by myocardial infarction. METHODS: Male Wistar rats were assigned to 2 groups: a simulated surgery group (SHAM; n = 8) and a myocardial infarction group (myocardial infarction; n = 13). After 3 months, morphological, functional and biochemical analyses were performed. The data are expressed as means±SD or medians (including the lower and upper quartiles). RESULTS: Myocardial infarctions induced increased left ventricular diastolic and systolic areas associated with a decreased fractional area change and a posterior wall shortening velocity. With regard to the extracellular matrix variables, the myocardial infarction group presented with higher values of periostin and types I and III collagen and higher interstitial collagen volume fractions and myocardial hydroxyproline concentrations. In addition, periostin was positively correlated with type III collagen levels (r = 0.673, p = 0.029) and diastolic (r = 0.678, p = 0.036) and systolic (r = 0.795, p = 0.006) left ventricular areas. Considering the relationship between periostin and the cardiac function variables, periostin was inversely correlated with both the fractional area change (r = -0.783, p = 0.008) and the posterior wall shortening velocity (r = -0.767, p = 0.012). CONCLUSIONS: Periostin might be a modulator of deleterious cardiac remodeling in the chronic phase after myocardial infarction in rats

Profile of Self-Care Capacity and Alcohol Use in Elderly Brazilians during the COVID-19 Outbreak: An Online Study

Background: Preventive and positive online coping strategies are essential for harm reduction associated with alcohol abuse among older adults in pandemic and social isolation scenarios. The objectives were to examine the relationship between alcohol use/abuse and physical capacity/self-care to perform the physical activities of daily living or impairment of the functional capacity of the elderly in the COVID-19 pandemic. Methods: An online cross-sectional survey was carried out. One hundred and one elderly people in the city of São Paulo, Brazil, participated in a community program. Results: Most participants (52.5%) showed excellent self-care skills. Approximately 12% of participants reported problems related to alcohol use/abuse. There was no association between self-care ability and abuse and probable alcohol dependence. Conclusions: Although most participants have excellent self-care and functional capacity and have not evidenced alcohol use/abuse, health professionals need to systematically provide information to prevent alcohol abuse, especially in scenarios of great emotional distress, such as in a pandemic. In addition, the online meetings held by the UAPI program were shown to be opportunities for social interaction and were essential to minimize the negative effects of the possible presence of alcohol use/abuse and the deteriorating performance of physical activities of daily living during a pandemic outbreak for the elderly