Cuantificación de las demandas de carrera en hockey hierba femenino mediante el uso de umbrales individualizados. [Quantification of the running demands in women`s field hockey using individualized thresholds].

El objetivo de este estudio fue analizar las demandas de carrera del hockey hierba femenino utilizando valores relativos para determinar los umbrales de intensidad. 15 jugadoras (23,7 ± 4,1 años) fueron monitorizadas en partidos de competición oficial, utilizando un sistema de posicionamiento global (GPS). Para determinar los umbrales relativos de intensidad de carrera se usaron el test 30-15IFT y la velocidad máxima de sprint. Los resultados muestran que existen diferencias significativas entre analizar la carrera de alta intensidad de las jugadoras de hockey con valores absolutos y valores relativos pasando de 479,8 ±134,9 m a 1116,3 ± 281,4 m); Tamaño del efecto ± 90% LC = 2,78 (2,17-3,38), distancias que se ajustan más a los esfuerzos de las jugadoras. Sin embargo, el número de sprint realizados disminuyó (de 6,26 a 3,59). Se concluye que el uso de umbrales relativos incrementa la distancia recorrida a alta intensidad en jugadoras de hockey hierba. Abstract The objective of this study was to analyze the running demands of women’s field hockey, using relative values to determine intensity thresholds. 15 players (23,7 ± 4,1 years) were monitored in official competition matches, using a global positioning system (GPS). To determine the thresholds relative to the intensity of the race, the 30-15IFT test and the maximum sprint speed were used. The results show that there are significant differences between the analysis of high-speed running of the hockey players with absolute and relative values, increasing from 479.8 ± 134,9 m to 1116,3 ± 281,4 m; Effect size ± 90% CL = 2,78 (2,17-3,38), distances that are more accurate to the player’s effort. However, the sprint number was decreased (from 6,26 to 3,59). The use of relative thresholds increases high-intensity running in women’s field hockey

Exploiting oxidative phosphorylation to promote the stem and immunoevasive properties of pancreatic cancer stem cells

© The Author(s) 2020Pancreatic ductal adenocarcinoma (PDAC), the fourth leading cause of cancer death, has a 5-year survival rate of approximately 7–9%. The ineffectiveness of anti-PDAC therapies is believed to be due to the existence of a subpopulation of tumor cells known as cancer stem cells (CSCs), which are functionally plastic, and have exclusive tumorigenic, chemoresistant and metastatic capacities. Herein, we describe a 2D in vitro system for long-term enrichment of pancreatic CSCs that is amenable to biological and CSC-specific studies. By changing the carbon source from glucose to galactose in vitro, we force PDAC cells to utilize OXPHOS, resulting in enrichment of CSCs defined by increased CSC biomarker and pluripotency gene expression, greater tumorigenic potential, induced but reversible quiescence, increased OXPHOS activity, enhanced invasiveness, and upregulated immune evasion properties. This CSC enrichment method can facilitate the discovery of new CSC-specific hallmarks for future development into targets for PDAC-based therapies.We acknowledge and thank Dr. Nuria Malats and Jaime Villarreal from the Spanish National Cancer Research Center (CNIO) for RNA sequencing and analysis, funded by Fondo de Investigaciones Sanitarias (FIS) grant PI18/01347. We thank Patricia Sánchez-Tomero and Marina Ochando-Garmendia for technical assistance and support and Dr. Raúl Sánchez Lanzas for assistance with autophagy experiments. We want to particularly acknowledge the patients and the BioBank Hospital Ramón y Cajal-IRYCIS (PT13/0010/0002) integrated in the Spanish National Biobanks Network for its collaboration and, in particular, Adrián Povo Retana for macrophage isolation. We would also like to thank the Transmission Electron Microscopy Unit Laboratory, part of the UAM Interdepartmental Investigation Service (SIdI); Coral Pedrero for exceptional help with in vivo experiments; and the laboratories of Dr. Amparo Cano and Dr. José González Castaño for reagents and helpful discussions. S.V. was a recipient of an Ayuda de Movilidad del Personal Investigador del IRYCIS, a mobility grant from the Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain, and a pre-doctoral fellowship from the Comunidad de Madrid, Ayudas Para La Contratación De Investigadores Predoctorales Y Posdoctorales (PEJD-2017-PRE/BMD-5062), Madrid, Spain. This study was supported by a Rámon y Cajal Merit Award (RYC-2012-12104) from the Ministerio de Economía y Competitividad, Spain (to B.S.); funding from la Beca Carmen Delgado/Miguel Pérez-Mateo from AESPANC-ACANPAN Spain (to B.S.); a Conquer Cancer Now Grant from the Concern Foundation (Los Angeles, CA, USA) (to B.S.); a Coordinated grant (GC16173694BARB) from the Fundación Asociación Española Contra el Cáncer (AECC) (to B.S.); FIS grants PI18/00757 (to B.S.), PI16/00789 (to M.A.F.-M.), PI18/00267 (to L.G.-B.; co-financed through Fondo Europeo de Desarrollo Regional (FEDER) “Una manera de hacer Europa”); a Miguel Servet award (CP16/00121) (to P.S.); a Max Eder Fellowship of the German Cancer Aid (111746) (to P.C.H.); and the German Research Foundation (DFG, CRC 1279 “Exploiting the human peptidome for Novel Antimicrobial and Anticancer Agents”; to P.C.H.)

Dietary α‐Linolenic Acid, Marine ω‐3 Fatty Acids, and Mortality in a Population With High Fish Consumption: Findings From the PREvención con DIeta MEDiterránea (PREDIMED) Study

Background: Epidemiological evidence suggests a cardioprotective role of α‐linolenic acid (ALA), a plant‐derived ω‐3 fatty acid. It is unclear whether ALA is beneficial in a background of high marine ω‐3 fatty acids (long‐chain n‐3 polyunsaturated fatty acids) intake. In persons at high cardiovascular risk from Spain, a country in which fish consumption is customarily high, we investigated whether meeting the International Society for the Study of Fatty Acids and Lipids recommendation for dietary ALA (0.7% of total energy) at baseline was related to all‐cause and cardiovascular disease mortality. We also examined the effect of meeting the society's recommendation for long‐chain n‐3 polyunsaturated fatty acids (≥500 mg/day). Methods and Results: We longitudinally evaluated 7202 participants in the PREvención con DIeta MEDiterránea (PREDIMED) trial. Multivariable‐adjusted Cox regression models were fitted to estimate hazard ratios. ALA intake correlated to walnut consumption (r=0.94). During a 5.9‐y follow‐up, 431 deaths occurred (104 cardiovascular disease, 55 coronary heart disease, 32 sudden cardiac death, 25 stroke). The hazard ratios for meeting ALA recommendation (n=1615, 22.4%) were 0.72 (95% CI 0.56–0.92) for all‐cause mortality and 0.95 (95% CI 0.58–1.57) for fatal cardiovascular disease. The hazard ratios for meeting the recommendation for long‐chain n‐3 polyunsaturated fatty acids (n=5452, 75.7%) were 0.84 (95% CI 0.67–1.05) for all‐cause mortality, 0.61 (95% CI 0.39–0.96) for fatal cardiovascular disease, 0.54 (95% CI 0.29–0.99) for fatal coronary heart disease, and 0.49 (95% CI 0.22–1.01) for sudden cardiac death. The highest reduction in all‐cause mortality occurred in participants meeting both recommendations (hazard ratio 0.63 [95% CI 0.45–0.87]). Conclusions: In participants without prior cardiovascular disease and high fish consumption, dietary ALA, supplied mainly by walnuts and olive oil, relates inversely to all‐cause mortality, whereas protection from cardiac mortality is limited to fish‐derived long‐chain n‐3 polyunsaturated fatty acids. Clinical Trial Registration URL: http://www.Controlled-trials.com/. Unique identifier: ISRCTN35739639

Formación inicial: reflexionando en torno a las construcciones de la diferencia

en torno a los conceptos de normalidad y diferencia expresados por los estudiantes de los dos últimos años de la carrera de Pedagogía en Inglés de la Universidad Metropolitana de Ciencias de la Educación (UMCE), futuros profesores que en su quehacer profesional ejercerán una influencia relevante en las subjetividades de sus estudiantes y en las posibilidades de aprendizaje de los mismos. Las dimensiones que se estudian son género, etnia y clase social. En este documento se comparten reflexiones preliminares en torno al análisis de los resultados de la validación de la metodología de esta investigación.This research, which is under development, seeks to understand the ideas around the concepts of normality and difference expressed by students in the last two years of the English teaching program at Universidad Metropolitana de Ciencias de la Educación (UMCE), future teachers who in their professional work will exert a major influence on the subjectivities of students and their possibilities of learning. The dimensions under the scope of our analysis are those of gender, ethnicity and social class. In this article, preliminary reflections on the analysis of the results of the validation of the methodology applied are shared

Impacto del uso de TIC en el desarrollo de habilidades de redacción en el idioma inglés como lengua extranjera

The present study aimed to explore the impact that the use of information and communication technologies (ICTs, henceforth) has on the development of the linguistic competence of EFL students of the TEFL Programme at UMCE. In order to do so, a quasi-experimental study was conducted: There was an experimental group taking the Inglés Escrito Pre-Intermedio course, who was exposed to ICT-based methodological strategies, and there was a control group. After the intervention, the results obtained in both groups were contrasted to identify differences. The results showed the effectiveness of the use of ICTs in the EFL students’ linguistic development. Therefore, this research seeks to create a positive impact on the design of ICT-based methodological strategies for the teacher training process, due to the relevance and pervasiveness these technologies have in our contemporary society.El presente estudio tuvo como objetivo explorar el impacto del uso de las tecnologías de la información y la comunicación (TIC, en adelante) en el desarrollo de la competencia lingüística de los estudiantes de ILE pertenecientes al programa de Pedagogía en Inglés de la UMCE. Para esto, un estudio cuasi-experimental se llevó a cabo donde un grupo experimental cursando Inglés Escrito Pre - Intermedio se expuso a estrategias metodológicas basadas en TIC, mientras que un grupo control no. Los resultados obtenidos en ambos grupos se contrastaron para identificar las posibles diferencias significativas. Los resultados mostraron la eficacia de la utilización de las TIC en el desarrollo lingüístico de los estudiantes. Por lo tanto, esta investigación busca crear un impacto positivo en el diseño de estrategias metodológicas basadas en TIC para la formación de profesores, debido a la relevancia y el uso generalizado que estas tecnologías tienen en nuestra sociedad contemporánea

Cuerpos y diversidades: desafíos encarnados

Depto. de Antropología Social y Psicología SocialDepto. de GeografíaFac. de Ciencias Políticas y SociologíaFac. de Geografía e HistoriaFALSEpu

Targeting cancer stem cell OXPHOS with tailored ruthenium complexes as a new anti-cancer strategy

Abstract Background Previous studies by our group have shown that oxidative phosphorylation (OXPHOS) is the main pathway by which pancreatic cancer stem cells (CSCs) meet their energetic requirements; therefore, OXPHOS represents an Achille’s heel of these highly tumorigenic cells. Unfortunately, therapies that target OXPHOS in CSCs are lacking. Methods The safety and anti-CSC activity of a ruthenium complex featuring bipyridine and terpyridine ligands and one coordination labile position (Ru1) were evaluated across primary pancreatic cancer cultures and in vivo, using 8 patient-derived xenografts (PDXs). RNAseq analysis followed by mitochondria-specific molecular assays were used to determine the mechanism of action. Results We show that Ru1 is capable of inhibiting CSC OXPHOS function in vitro, and more importantly, it presents excellent anti-cancer activity, with low toxicity, across a large panel of human pancreatic PDXs, as well as in colorectal cancer and osteosarcoma PDXs. Mechanistic studies suggest that this activity stems from Ru1 binding to the D-loop region of the mitochondrial DNA of CSCs, inhibiting OXPHOS complex-associated transcription, leading to reduced mitochondrial oxygen consumption, membrane potential, and ATP production, all of which are necessary for CSCs, which heavily depend on mitochondrial respiration. Conclusions Overall, the coordination complex Ru1 represents not only an exciting new anti-cancer agent, but also a molecular tool to dissect the role of OXPHOS in CSCs. Results indicating that the compound is safe, non-toxic and highly effective in vivo are extremely exciting, and have allowed us to uncover unprecedented mechanistic possibilities to fight different cancer types based on targeting CSC OXPHOS

El gran juego de la paz

El trabajo obtuvo el Tercer Premio de la Modalidad B de los 'Premios Tomás García Verdejo' a las buenas prácticas educativas en la Comunidad Autónoma de Extremadura para el curso académico 2013/2014Se presentan un conjunto de actividades que se han llevado a cabo en el IESO Matías Ramón Martínez de Burguillos del Cerro (Badajoz) desde el curso 2007-2008 para la conmemoración del Día de la no violencia y la paz y con el desarrollo de 'El gran juego de la paz'. Entre los objetivos que se perseguían estaban: promocionar valores de paz, igualdad y no violencia entre los alumnos, promover la educación para la salud, el fomento de la práctica deportiva y el respeto por el medio ambiente, adquirir una buena inteligencia emocional, despertar el gusto e interés por la lectura y la investigación en la comunidad educativa, promover el uso de las nuevas tecnologías, desarrollar el plurilingüísmo en el alumnado, potenciar la integración escolar, aprender a resolver conflictos escolares de manera pacífica y democrática, fomentar la participación e implicación de la familia, profesorado, asociaciones locales, ayuntamiento, etc. en la vida escolar del centro y crear un clima de convivencia idóneo entre todos los sectores de la comunidad escolarExtremaduraES

Down syndrome as risk factor for respiratory syncytial virus hospitalization : A prospective multicenter epidemiological study

Respiratory syncytial virus (RSV) infection in childhood, particularly in premature infants, is associated with significant morbidity and mortality. To compare the hospitalization rates due to RSV infection and severity of disease between infants with and without Down syndrome (DS) born at term and without other associated risk factors for severe RSV infection. In a prospective multicentre epidemiological study, 93 infants were included in the DS cohort and 68 matched by sex and data of birth (±1 week) and were followed up to 1 year of age and during a complete RSV season. The hospitalization rate for all acute respiratory infection was significantly higher in the DS cohort than in the non-DS cohort (44.1% vs 7.7%, P<.0001). Hospitalizations due to RSV were significantly more frequent in the DH cohort than in the non-DS cohort (9.7% vs 1.5%, P=.03). RSV prophylaxis was recorded in 33 (35.5%) infants with DS. The rate of hospitalization according to presence or absence of RSV immunoprophylaxis was 3.0% vs 15%, respectively. Infants with DS showed a higher rate of hospitalization due to acute lower respiratory tract infection and RSV infection compared to non-DS infants. Including DS infants in recommendations for immunoprophylaxis of RSV disease should be considered