Evaluation of Gyp7 Protein Ability to Coordinate and Regulate Mitochondrial Genomes Stability

Cellular creation of adenosine triphosphate, ATP, is essential for eukaryotic cells to function properly. The ATP molecule drives most of the biochemical and metabolic pathways of the cell. The cell\u27s ATP is produced in the mitochondria. Mutations within the genome of the mitochondria will alter the cell\u27s ability to generate A TP. Preliminary work has shown that loss of the Gyp 7p in Saccharomyces cerevisiae blocks the ability of mitochondria to properly function. The Gyp 7 gene was isolated using a technique called two-hybrid analysis with a known mitochondrial protein called llvSp, which was used as \u27bait\u27. We have shown that a deletion of the Gyp7 gene is not essential for cellular viability in S. cerevisiae. We observed that loss of Gyp 7 decreases both the occurrence of point mutations at microsatellite sequences as well as decreasing the rate at which recombination between direct-repeat DNA sequences occurs. This contributes to the effective that cellular respiration mutation rate increase when Gyp7p is removed. Gyp7 encodes for the production of the GTPase-activating protein (GAP) Gyp7p within the Ypt/Rab transport GTPase pathway. This pathway is involved in protein trafficking within the cell

Stimulant effects of ethanol in adolescent Swiss mice: development of sensitization and consequences in adulthood

The adolescent period is characterized by behavioral and neurobiological changes, which might predispose adolescents to the long-term negative consequences of alcohol. For example, enhanced risks of alcohol dependence are reported when drinking is initiated early. In the present studies, we used Swiss female mice to test whether chronic ethanol injections during adolescence durably affect the sensitivity to the stimulant effects of ethanol in adulthood. In a first set of experiments, several groups of young (28 day-old) mice were daily injected with various ethanol doses (1.5 – 4 g/kg) to test for ethanol sensitization during adolescence in comparison to adult mice exposed to the same schedule of ethanol injections. The results show that young mice express much higher stimulant effects after acute ethanol injections. However, they also require higher ethanol doses than adult mice to develop a sensitization to the stimulant effects of ethanol. In a second set of experiments, 28 day-old mice were sensitized to ethanol for 14 days with high ethanol doses (2.5 or 4 g/kg) and then tested for the stimulant effects of ethanol and the development of ethanol sensitization in adulthood. The results of this second set of experiments show that mice sensitized to ethanol during their adolescence remain more sensitive to the acute stimulant effects of ethanol in adulthood, especially when high ethanol doses were administered. However, the rate of the development of a sensitization to this effect was only slightly affected relative to adult mice exposed to a chronic ethanol regimen for the first time. Together, these results indicate that adolescent mice are more sensitive to the stimulant effects of ethanol but require higher ethanol doses to develop a sensitization. However, when a sensitization develops during adolescence, these mice still experience higher ethanol stimulant effects when tested in adulthood.Peer reviewe

Exploring and Validating LM Performance at Very High Resolution

Numerical weather prediction models will in the near future be operationally run at resolutions of the order of l-3km even at the Alpine scale. It is the purpose of this study to investigate the potential benefits and also the problems involved with this increase in resolution. To this end high resolution simulations (at 2km) with the Lokal Model] (LM) of the COSMO1 are performed for selected MAP IOP cases and the results are examined in comparison with available high resolution datasets from the MAP campaign and with operational simulations at coarser resolution. The study develops in three directions: (i) study of meso-scale structures with help of a new modeling tool (Dynamics), (ii) with a systematical analysis of the model behavior for a selection of MAP IOP case studies with particular attention to model errors in precipitation field (Prediction) and (iii) experiments with the numerical set-up of the model (Numerics) investigating the influence of the domain size on the high resolution simulation and adapting the number of vertical levels of the model due to the increased horizontal resolution

Estudo bibliométrico sobre as publicações na área de finanças comportamentais no Brasil entre 2001 e 2017

Este artigo busca verificar a evolução da produção acadêmica brasileira em finanças comportamentais através de um estudo bibliométrico utilizando um software que coleta dados diretamente do Currículo Lattes dos pesquisadores em finanças e a busca por palavra-chave nos dez principais periódicos da área no país. Identificamos 47 publicações na área de finanças comportamentais no Brasil, no período de 2001 a 2017, distribuídas em 28 periódicos. A maior parte das publicações ocorreu entre 2007 e 2013, o que pode indicar o aumento no interesse em fenômenos atípicos durante a crise econômica. A região Sudeste foi a que mais apresentou estudos sobre o tema, com destaque para o estado de São Paulo. Os estudos foram realizados por um total de 118 pesquisadores, sendo média de autores por artigo foi de 3,28. Avaliamos também o Qualis dos periódicos nas áreas de Administração Pública e de Empresas, Ciências contábeis e Turismo e de Economia e por fim apresentamos as considerações finais, incluindo sugestões para estudos futuros