A Rare Case of Mediastinal Mass: Thymoma and Thymic Tumor

Thymomas and thymic carcinomas are rare mediastinal neoplasms arising from thymic epithelial cells, and the presence of synchronous or metachronous primary thymic neoplasms in a single patient is an extremely rare event. Thymoma patients appear to have an inherent predisposition toward developing additional neoplasms. This additionally presents a diagnostic challenge, revealing the importance of multidisciplinary expertise to the management of these patients. This is a case report of a patient with a thymoma and thymic carcinoma, submitted to surgical resection and postoperative radiotherapyinfo:eu-repo/semantics/publishedVersio

Modulation of dietary protein to lipid ratios for gilthead seabream on-growing during summer temperature conditions

Gilthead seabream (Sparus aurata) tend to increase fat deposition during summer farming conditions in the Mediterranean, which may negatively affect productive performance and consumers' quality perception of the final product. Therefore, this study evaluated the impacts of protein to lipid ratios in low fishmeal/fish oil diets on growth performance, body composition, feed conversion and nutrient utilization of seabream on-grown during summer temperature conditions. The experimental diets contained low levels of fishmeal, fish oil, and crude protein (39%), differing in crude lipid content: 16% (MF diet) or 12% (LF diet). A growth trial was per-formed with seabream (initial weight: 100 & PLUSMN; 7 g) from August to October (water temperature: 23.1 & PLUSMN; 2.2 & DEG;C). A digestibility trial was also performed (at 23 & DEG;C). Key performance indicators, whole-body composition and ac-tivities of digestive enzymes were evaluated at the end of the experiment (64 days). Low dietary lipid levels negatively affected lipid, energy, and amino acid digestibility, and as a result, fish fed the LF diet presented higher nitrogen faecal losses. Still, the decrease in nutrient digestibility was not related to dietary effects on the digestive enzyme activities. The experimental diets did not compromise the activity of pancreatic, gastric, and intestinal digestive enzymes nor feed utilization, but a slight growth impairment was observed in fish fed the LF diet, probably due to the lower amino acid and lipid digestibility. However, a potential benefit of this dietary treatment towards reducing fat accumulation in seabream during summer was observed. Nevertheless, the environmental impact of the nitrogen losses during seabream on-growing should be considered when estimating the sustainability of the production. This study demonstrated that the optimisation of diet formulations should account for the environmental conditions, especially in Mediterranean aquaculture, so the economic and envi-ronmental impacts may be correctly evaluated towards a more sustainable fish production.info:eu-repo/semantics/publishedVersio

Comparing Soil Compaction under Different Grazing Systems with a Virgin Forest Soil to Determine Optimal Stocking Rates

The understanding of how soil physical properties respond to differing grazing practices may help explain the main causes of pasture degradation. Soil compaction has been shown to be a main degradation form of soil and the knowledge of techniques to quantify and rectify this are necessary to maintain optimal yields. This research aims to measure the rupture lines of red yellow latossol under differing pasture grazing practices compared to cropping and a natural forest. With this information it is aimed to calculate the correction factor for stocking rates and traffic of tillage tools. The differing soil management practices examined was, pasture grazed by sheep, and dairy cattle, a maize crop in no tillage cover-crop system and a natural forest. To quantify the soil physical changes, the direct shear test was used, which calculated the resultant force of a load. The resultant forces of the natural forest were compared against pasture systems and crop system, and a correction factor for stocking rates was calculated. The samples of Red yellow Latossol were equilibrated in the matrix potential (ψ): -6 kPa. In the shear test, the normal stress used was the 450kPa. The correction factor (CF) indicates whether the soil has structural degradation compared to natural forest. Values less than 1 indicated soil degradation. The pastures grazed by sheep and dairy cattle had values observed to be less than 1, excessive loads at high soil moisture may be attributed to this soil structural deformation. For these systems, grazing management and stocking rates should be corrected. The correction factor gives an indication of the magnitude of management change that is required (i.e. the stocking rate decreased). The crop area was found to have no soil strength issues, using the stress test

In situ enabling approaches for tissue regeneration: current challenges and new developments

In situ tissue regeneration can be defined as the implantation of tissue-specific biomaterials (by itself or in combination with cells and/or biomolecules) at the tissue defect, taking advantage of the surrounding microenvironment as a natural bioreactor. Up to now, the structures used were based on particles or gels. However, with the technological progress, the materials’ manipulation and processing has become possible, mimicking the damaged tissue directly at the defect site. This paper presents a comprehensive review of current and advanced in situ strategies for tissue regeneration. Recent advances to put in practice the in situ regeneration concept have been mainly focused on bioinks and bioprinting techniques rather than the combination of different technologies to make the real in situ regeneration. The limitation of conventional approaches (e.g., stem cell recruitment) and their poor ability to mimic native tissue are discussed. Moreover, the way of advanced strategies such as 3D/4D bioprinting and hybrid approaches may contribute to overcome the limitations of conventional strategies are highlighted. Finally, the future trends and main research challenges of in situ enabling approaches are discussed considering in vitro and in vivo evidence.info:eu-repo/semantics/publishedVersio

New terrestrial cyanobacteria from the Azores Islands: description of Venetifunis gen. nov. and new species of Albertania, Kovacikia and Pegethrix

The taxonomy of cyanobacteria has advanced quickly with the use of molecular methods in combination with well-defined morphological and ecological characters. Using this approach, many taxonomical changes have occurred in the Synechococcales and Oscillatoriales, with the description of new families, genera and species. Here we present the study of four cyanobacterial strains from the Bank of Algae and Cyanobacteria of the Azores (BACA) culture collection, all isolated from the Azores Archipelago. The strains were genetically characterized through the amplification of the 16S rRNA gene and 16S-23S rRNA internal transcribed spacer (ITS) region, as well as morphologically by light and transmission electron microscopy. One of the strains presented a high genetic divergence from known genera of Synechococcales and was described as Venetifunis florensis gen. & sp. nov. (Trichocoleusaceae, Cyanobacteria). The remaining three strains were found to be new species of the genera Albertania, Kovacikia and Pegethrix. Venetifunis florensis was isolated from the shores of Lake Rasa in Flores Island, Kovacikia atmophytica sp. nov. is a new species mainly distinguished genetically from other Kovacikia species from Terceira Island. Albertania obscura sp. nov. was isolated from inside a volcanic cave in Terceira Island and is distinct both morphologically and genetically from the already described species. Pegethrix atlantica sp. nov. is a new morphologically and genetically different species isolated from São Miguel Island. The description of these new taxa increases the biogeographic knowledge of the four mentioned cyanobacterial genera to remote oceanic islands and new terrestrial habitats

Continuous production of highly tuned silk/calcium-based composites: exploring new pathways for skin regeneration

Calcium plays an important role in barrier function repair and skin homeostasis. In particular, calcium phosphates (CaPs) are well established materials for biomedical engineering due to their biocompatibility. To generate biomaterials with a more complete set of biological properties, previously discarded silk sericin (SS) has been recovered and used as a template to grow CaPs. Crucial characteristics for skin applications, such as antibacterial activity, can be further enhanced by doping CaPs with cerium (Ce) ions. The effectiveness of cell attachment and growth on the materials highly depends on their morphology, particle size distribution, and chemical composition. These characteristics can be tailored through the application of oscillatory flow technology, which provides precise mixing control of the reaction medium. Thus, in the present work, CaP/SS and CaP/SS/Ce particles were fabricated for the first time using a modular oscillatory flow plate reactor (MOFPR) in a continuous mode. Furthermore, the biological behavior of both these composites and of previously produced pure CaPs was assessed using human dermal fibroblasts (HDFs). It was demonstrated that both CaP based with plate-shaped nanoparticles and CaP-SS-based composites significantly improved cell viability and proliferation over time. The results obtained represent a first step towards the reinvention of CaPs for skin engineering.info:eu-repo/semantics/publishedVersio

Uncovering archaeological sites in airborne LiDAR data with data-centric artificial intelligence

Mapping potential archaeological sites using remote sensing and artificial intelligence can be an efficient tool to assist archaeologists during project planning and fieldwork. This paper explores the use of airborne LiDAR data and data-centric artificial intelligence for identifying potential burial mounds. The challenge of exploring the landscape and mapping new archaeological sites, coupled with the difficulty of identifying them through visual analysis of remote sensing data, results in the recurring issue of insufficient annotations. Additionally, the top-down nature of LiDAR data hinders artificial intelligence in its search, as the morphology of archaeological sites blends with the morphology of natural and artificial shapes, leading to a frequent occurrence of false positives. To address this problem, a novel data-centric artificial intelligence approach is proposed, exploring the available data and tools. The LiDAR data is pre-processed into a dataset of 2D digital elevation images, and the known burial mounds are annotated. This dataset is augmented with a copy-paste object embedding based on Location-Based Ranking. This technique uses the Land-Use and Occupation Charter to segment the regions of interest, where burial mounds can be pasted. YOLOv5 is trained on the resulting dataset to propose new burial mounds. These proposals go through a post-processing step, directly using the 3D data acquired by the LiDAR to verify if its 3D shape is similar to the annotated sites. This approach drastically reduced false positives, attaining a 72.53% positive rate, relevant for the ground-truthing phase where archaeologists visit the coordinates of proposed burial mounds to confirm their existence.This work was supported by the Project Odyssey: Platform for Automated Sensing in Archaeology Co-Financed by COMPETE 2020 and Regional Operational Program Lisboa 2020 through Portugal 2020 and FEDER under Grant ALG-01-0247-FEDER-070150.info:eu-repo/semantics/publishedVersio