Not Simply Green: Nature-Based Solutions as a Concept and Practical Approach for Sustainability Studies and Planning Agendas in Cities

The concept of a nature-based solution (NBS) has been developed in order to operationalize an ecosystem services approach within spatial planning policies and practices, to fully integrate the ecological dimension, and, at the same time, to address current societal challenges in cities. It exceeds the bounds of traditional approaches that aim ‘to protect and preserve’ by considering enhancing, restoring, co-creating, and co-designing urban green networks with nature that are characterized by multifunctionality and connectivity. NBSs include the main ideas of green and blue infrastructure, ecosystem services, and biomimicry concepts, and they are considered to be urban design and planning tools for ecologically sensitive urban development. Nowadays, NBSs are on their way to the mainstream as part of both national and international policies. The successful implementation of NBSs in Europe and worldwide, which is becoming increasingly common, highlights the importance and relevance of NBS for sustainable and livable cities. This paper discusses the roles, development processes, and functions of NBSs in cities by taking Leipzig as a case study. Using data from interviews conducted from 2017 to 2019, we study the past and current challenges that the city faces, including the whole process of NBS implementation and successful realization. We discuss the main drivers, governance actors, and design options of NBSs. We highlight the ecosystem services provided by each NBS. We discuss these drivers and governance strategies by applying the framework for assessing the co-benefits of NBSs in urban areas in order to assess the opportunities and challenges that NBSs may have. This way, we are able to identify steps and procedures that help to increase the evidence base for the effectiveness of NBS by providing examples of best practice that demonstrate the multiple co-benefits provided by NBSs.Peer Reviewe

Lawns in Cities: From a Globalised Urban Green Space Phenomenon to Sustainable Nature-Based Solutions

This opinion paper discusses urban lawns, the most common part of open green spaces and urban green infrastructures. It highlights both the ecosystem services and also disservices provided by urban lawns based on the authors’ experience of working within interdisciplinary research projects on lawns in different cities of Europe (Germany, Sweden and Russia), New Zealand (Christchurch), USA (Syracuse, NY) and Australia (Perth). It complements this experience with a detailed literature review based on the most recent studies of different biophysical, social, planning and design aspects of lawns. We also used an international workshop as an important part of the research methodology. We argue that although lawns of Europe and the United States of America are now relatively well studied, other parts of the world still underestimate the importance of researching lawns as a complex ecological and social phenomenon. One of the core objectives of this paper is to share a paradigm of nature-based solutions in the context of lawns, which can be an important step towards finding resilient sustainable alternatives for urban green spaces in the time of growing urbanisation, increased urban land use competition, various user demands and related societal challenges of the urban environment. We hypothesise that these solutions may be found in urban ecosystems and various local native plant communities that are rich in species and able to withstand harsh conditions such as heavy trampling and droughts. To support the theoretical hypothesis of the relevance of nature-based solutions for lawns we also suggest and discuss the concept of two natures—different approaches to the vision of urban nature, including the understanding and appreciation of lawns. This will help to increase the awareness of existing local ecological approaches as well as an importance of introducing innovative landscape architecture practices. This article suggests that there is a potential for future transdisciplinary international research that might aid our understanding of lawns in different climatic and socio-cultural conditions as well as develop locally adapted (to environmental conditions, social needs and management policies) and accepted nature-based solutions.Peer Reviewe

Editorial for Special Issue “Nature-Based Solutions (NBS) in Cities and Their Interactions with Urban Land, Ecosystems, Built Environments and People: Debating Societal Implications”

Peer Reviewe

Urban Cemeteries—Places of Multiple Diversity and Challenges. A Case Study from Łódź (Poland) and Leipzig (Germany)

This article presents a pilot study investigating the multidimensional diversity of cemeteries as an important element of cultural heritage and green infrastructure within the urban landscape. We studied the state and diversity of nature, perceptions, and activities of visitors. As religion is an important aspect that differentiates cemeteries from each other, we studied a sample of four multi-confessional urban cemeteries in Łódź (Poland) and Leipzig (Germany) by using site observation and a questionnaire survey. We found that cemeteries are far undervalued as public green resources that can perform important functions in sociocultural life and the mental well-being of the general public, as the perceptions of silence- and contemplation-seeking visitors tell us. The perception of cemeteries depends on the level of secularization, varying from a sacrum sphere up to specific recreational and touristic opportunities; findings that should be considered by town planners when optimizing the cultural ecosystem services of green spaces.Peer Reviewe

Innovative e-Learning Training Modules to Improve Animal Welfare during Transport and Slaughter of Pigs: A Pretest–Posttest Study to Pre-Evaluate the General Didactical Concept

In addition to the information on the possession of a certificate of competence, there are no concrete obligations for repetitive training for personnel handling live animals at transport and slaughter. Deficiencies in the animal-welfare-friendly handling of pigs are known. The developed pilot modules “Handling of pigs” and “Electrical stunning” were tested in a pretest–posttest study in German and Romanian using questions of knowledge before and after the implementation of the modules. In this study, 45 and 46 datasets of participants could be analyzed. The mean percentages of correctly answered questions in the posttest increased by 5.6% in the module “Handling of pigs” and by 10.6% in the module “Electrical stunning”. A significant interaction was found for the language match and trend categories in the module “Handling of pigs”. No Romanian native speaker had a positive trend in this module. For both modules separately, participant education level significantly interacted with the language match and the presence or absence of a certificate of competence. Comparing the percentages of the correct given answers, significant interactions in the subgroups were more common in the module “Electrical stunning”. One question in “Electrical stunning” was correctly answered significantly more often in the posttest. Because of the positive mean trends of knowledge within this pre-evaluation, we assume the didactical concept was suitable for our target groups. Holders of a certificate of competence also gave more correct answers in the post-test. This underlines the importance of repetitive training. Differences in the trends of knowledge gain seem to be topic and experience related

Glucocorticoids Equally Stimulate Epithelial Na+ Transport in Male and Female Fetal Alveolar Cells

Preterm infants frequently suffer from respiratory distress syndrome (RDS), possibly due to lower expression of epithelial Na+ channels (ENaC). RDS incidence is sex-specific, affecting males almost twice as often. Despite the use of antenatal glucocorticoids (GCs), the sex difference persists. It is still controversial whether both sexes benefit equally from GCs. We previously showed that Na+ transport is higher in female compared with male fetal distal lung epithelial (FDLE) cells. Since GCs increase Na+ transport, we hypothesized that their stimulating effect might be sex-specific. We analyzed FDLE cells with Ussing chambers and RT-qPCR in the presence or absence of fetal serum. In serum-free medium, GCs increased the ENaC activity and mRNA expression, independent of sex. In contrast, GCs did not increase the Na+ transport in serum-supplemented media and abolished the otherwise observed sex difference. Inhibition of the GC receptor in the presence of serum did not equalize Na+ transport between male and female cells. The GC-induced surfactant protein mRNA expression was concentration and sex-specific. In conclusion, female and male FDLE cells exhibit no sex difference in response to GCs with regard to Na+ transport, and GR activity does not contribute to the higher Na+ transport in females

Spatial and temporal effects of heat waves on the diversity of European stream invertebrate communities

The frequency and magnitude of extreme events, such as heat waves, are predicted to increase with climate change. However, assessments of the response of biological communities to heat waves are often inconclusive. We aimed to assess the responses in abundance, taxonomic and functional diversity indices of stream invertebrate communities to heat waves using long-term monitoring data collected across Europe. We quantified the heat waves' magnitude, analyzed the spatial (i.e., long-term mean) and temporal (anomaly around the long-term mean) components of variation in the magnitude of heat waves, and their interaction with anthropogenic stressors (ecological quality and land cover). For the spatial component of variation, we found a negative association of the community indices to the increasing magnitude of heat waves. Sites undergoing heat waves of higher magnitude showed fewer species and lower trait diversity compared with sites experiencing lower magnitude heat waves. However, we could not detect an immediate temporal response of the communities to heat waves (i.e., the temporal component). Furthermore, we found that the effects of heat waves interacted with the ecological quality of the streams and their surrounding land cover. Diversity declined with increasing heat waves' magnitude in streams with higher ecological quality or surrounded by forest, which may be due to a higher proportion of sensitive species in the community. Heat waves' impacts on diversity were also exacerbated by increasing urban cover. The interaction between heat waves' magnitude and anthropogenic stressors suggests that the effects of extreme events can compromise the recovery of communities. Further, the predicted increase in heat waves will likely have long-term effects on stream invertebrate communities that are currently undetected

Labeling of Anti-MUC-1 Binding Single Chain Fv Fragments to Surface Modified Upconversion Nanoparticles for an Initial in Vivo Molecular Imaging Proof of Principle Approach

In vivo optical Imaging is an inexpensive and highly sensitive modality to investigate and follow up diseases like breast cancer. However, fluorescence labels and specific tracers are still works in progress to bring this promising modality into the clinical day-to-day use. In this study an anti-MUC-1 binding single-chain antibody fragment was screened, produced and afterwards labeled with newly designed and surface modified NaYF4:Yb,Er upconversion nanoparticles as fluorescence reporter constructs. The MUC-1 binding of the conjugate was examined in vitro and in vivo using modified state-of-the-art small animal Imaging equipment. Binding of the newly generated upconversion nanoparticle based probe to MUC-1 positive cells was clearly shown via laser scanning microscopy and in an initial proof of principal small animal optical imaging approach

Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells

In vitro differentiation of human pluripotent stem cells (hPSCs) recapitulates early aspects of human embryogenesis, but the underlying processes are poorly understood and controlled. Here we show that modulating the bulk cell density (BCD: cell number per culture volume) deterministically alters anteroposterior patterning of primitive streak (PS)-like priming. The BCD in conjunction with the chemical WNT pathway activator CHIR99021 results in distinct paracrine microenvironments codifying hPSCs towards definitive endoderm, precardiac or presomitic mesoderm within the first 24 h of differentiation, respectively. Global gene expression and secretome analysis reveals that TGFß superfamily members, antagonist of Nodal signalling LEFTY1 and CER1, are paracrine determinants restricting PS progression. These data result in a tangible model disclosing how hPSC-released factors deflect CHIR99021-induced lineage commitment over time. By demonstrating a decisive, functional role of the BCD, we show its utility as a method to control lineage-specific differentiation. Furthermore, these findings have profound consequences for inter-experimental comparability, reproducibility, bioprocess optimization and scale-up.DFG/REBIRTHDFG/EXC62/1DFG/ZW 64/4-1DFG/MA 2331/16-1BMBF/13N12606BMBF/StemBANCCEU H2020/66872