Chromosome Characterization of Bartek (Cucumis Melo L. var. Bartek), Local Melon Variety from Pemalang

ABSTRACTBartek is one of local melon varieties which mainly cultivated in Pemalang, Central Java. Bartek has three shapes of fruit variation; Long-Green, Ellipse-Green, and Yellow. Chromosome characterization of the Bartek was investigated to determine the genetic variation. The main purpose of this research was to determine the genetic characters of Bartek including chromosome number, mitosis, cell cycle, and karyotype. Squash method was used for chromosome preparation. The results showed that all of Bartek observed in this study have similar diploid (2n) chromosome number = 24. According to total number of chromosome, Bartek is more related to melon. The mitotic analysis exhibited that the Bartek has same Karyotype formula, 2n = 2x = 24m. According to the R value of the three kind of Bartek (R< 0.27), it has indicated that three kind of Bartek were considered to be originated from same species and one of melon varieties (Cucumis melo L. var. Bartek).Key words: cucumber, bartek, chromosome, karyotyp

Kajian aspek keamanan nyamuk Aedes aegypti Linnaeus ber-Wolbachia di Yogyakarta, Indonesia

Dengue prevention efforts are limited to the control strategies of its vector and the management of breeding sites. New alternatives for dengue vector control that are sustainable and more environmentally friendly are needed to complement the government’s current efforts. Research on Wolbachia-infected Aedes aegypti Linnaeus mosquitoes as an alternative biocontrol strategy has been performed in Yogyakarta City. However, one of the concerns of the community members and stakeholders about this technology is the safety aspect regarding the transmission of Wolbachia to other species and the possibility that humans will contract Wolbachia. This study aimed to address these concerns, namely to find out whether horizontal transmission of Wolbachia occurred from A. aegypti that were released to other species and whether residents living in the released areas were infected with Wolbachia. The research was conducted in Dusun Nogotirto and Dusun Kronggahan (Sleman Regency), as well as in Dusun Jomblangan and Dusun Singosaren (Bantul Regency), Yogyakarta Special Province. Wolbachia qPCR screening using the target gene WD0513 was performed on 922 Culex quinquefasciatus Say and 331 Aedes albopictus (Skuse). ELISA test was carried out on 190 pairs of plasma samples, namely the sample before the Wolbachia frequency was established (still 80%). The results showed no evidence of Wolbachia transfer from Wolbachia-infected A. aegypti to other mosquito species coexisting in the same habitat or to humans. This study corroborates the safety evidence of Wolbachia-infected A. aegypti technology as an alternative to control dengue virus transmissio

Bartek is one of local melon varieties mainly cultivated in Pemalang, Central Java. Bartek has three variations of fruits; Long-Green, Ellips-Green, and Yellow. Chromosome characterization of the Bartek was investigated to determine the genetic variation. The main purpose of this research was to determine the genetic characters of Bartek including chromosome number, mitosis, cell cycle, and karyotype. Squash method was used for chromosome preparation. The results showed that all of Bartek observed in this study have similar diploid (2n) chromosome number = 24. According to the total number of chromosome, Bartek is closer to melon than cucumber. The mitotic analysis exhibited that the Bartek has similar karyotype formula, 2n = 2x = 24m. Based on the R value of the three kinds of Bartek (R &lt; 0.27), it indicated that three kinds of Bartek were considered to be originated from similar species and one of melon varieties (Cucumis melo L. var. Bartek).

Bartek is one of local melon varieties mainly cultivated in Pemalang, Central Java. Bartek has three variations of fruits; Long-Green, Ellips-Green, and Yellow. Chromosome characterization of the Bartek was investigated to determine the genetic variation. The main purpose of this research was to determine the genetic characters of Bartek including chromosome number, mitosis, cell cycle, and karyotype. Squash method was used for chromosome preparation. The results showed that all of Bartek observed in this study have similar diploid (2n) chromosome number = 24. According to the total number of chromosome, Bartek is closer to melon than cucumber. The mitotic analysis exhibited that the Bartek has similar karyotype formula, 2n = 2x = 24m. Based on the R value of the three kinds of Bartek (R &lt; 0.27), it indicated that three kinds of Bartek were considered to be originated from similar species and one of melon varieties (Cucumis melo L. var. Bartek)

Chromosome Characterization of Bartek (Cucumis Melo L. var. Bartek), Local Melon Variety from Pemalang

ABSTRACTBartek is one of local melon varieties which mainly cultivated in Pemalang, Central Java. Bartek has three shapes of fruit variation; Long-Green, Ellipse-Green, and Yellow. Chromosome characterization of the Bartek was investigated to determine the genetic variation. The main purpose of this research was to determine the genetic characters of Bartek including chromosome number, mitosis, cell cycle, and karyotype. Squash method was used for chromosome preparation. The results showed that all of Bartek observed in this study have similar diploid (2n) chromosome number = 24. According to total number of chromosome, Bartek is more related to melon. The mitotic analysis exhibited that the Bartek has same Karyotype formula, 2n = 2x = 24m. According to the R value of the three kind of Bartek (R< 0.27), it has indicated that three kind of Bartek were considered to be originated from same species and one of melon varieties (Cucumis melo L. var. Bartek).Key words: cucumber, bartek, chromosome, karyotyp

VARIASI GENETIK POPULASI Vanda foetida J.J.S. DI GUNUNG DEMPO DAN PADIAMPE, SUMATERA SELATAN BERDASARKAN KARAKTER MOLEKULAR

It Vanda foetida J.J.S is an endemic orchid in Sumatra which first described in 1906 by J.J. Smith. was first found in Palembang, South Sumatra. Since described, V. foetida has not been seen in its habitat and the world�s orchid collection until recent time. Thus, it is assumed that this species was taxonomically disappeared more than 100 years. The distribution of V. foetida was limited in the habitat because of the deep exploitation of this orchid. In 2010, V. foetida was found in Mount Dempo and Padiampe, South Sumatra. Coffee plantation and over-exploitation to fulfill commercial demand have become serious threats for V. foetida population that is now critically endangered. In-situ and ex-situ conservation patterns are needed to maintain the existence of V. foetida continuously. andom A The aim of this research was to study the genetic variation of V. foetida in its natural habitat, Mount Dempo and Padiampe, South Sumatra. Based on R mplified Polymorphic DNA (RAPD) analysis for genetic variation using 8 primers (OPU 3, OPU 5, OPU 6, OPU 7, OPU 12, OPU 13, OPU 14, and OPU 16), it is known that V. foetida originated from two populations were forming two main clusters in dendrogram with 67% (0,67) degree of similarity. Two populations of V. foetida at recent time were separated from larger population caused by habitat fragmentation. This phenomenon was showed by the grouping of individuals from both populations into one cluster in dendrogram. Two dendrogram clusters which showed low degree of similarity had revealed that the simpatric speciation occured in those populations. Genetic variation in each population was detected by the value of h (Nei�s genetic diversity) which results were 0,1999 and 0,1778 for Mount Dempo and Padiampe, respectively. The information of genetic variation can be used to support the V. foetida conservation, which mainly focussed on increasing the number of individuals and also enriching the genetic variation to maintain the existence of V. foetida populations

Simple Feasibility Analysis Of Nitrogen-Fixing Cereals Project

Nitrogen does not directly have advantages in human physiology system, but it holds one of the most critical roles in plants’ life cycle and productivity. Even though Nitrogen is the most abundant elements in the atmosphere, it is also the most deficient essential nutrients in plants. The proposed idea of the nitrogen-fixing GM crops, particularly wheat, is aimed to overcome those stated cons of the traditional diculture and nitrogen fertilizer. This analysis focus on the overview as well as the pro and cons of the genetically modified nitrogen-fixing plants in providing a better agricultural method. The genetically modifying method to generate a nitrogen-fixing non-legumes carries a significant chance of failure results and hindrance. The multilevel implication occurs when we need to modify the plants that not normally produce nodules in their roots to form the nodules and to modify the Nitrogen-fixing microbes to live in the nodules of non-legumes, which are not their natural dwelling places.In conclusion, the genetically modified crops project to fix their Nitrogen is feasible, but the difficulties and the funds needed still outweigh the benefits obtained in the future. With all of those limitations, the target goal to erase famine in 2050 just by funding the nitrogen-fixing wheat alone seems to be too high to be reached. The funds and efforts should be better spent on other factors and farming methods

Simple Feasibility Analysis Of Nitrogen-Fixing Cereals Project

Nitrogen does not directly have advantages in human physiology system, but it holds one of the most critical roles in plants’ life cycle and productivity. Even though Nitrogen is the most abundant elements in the atmosphere, it is also the most deficient essential nutrients in plants. The proposed idea of the nitrogen-fixing GM crops, particularly wheat, is aimed to overcome those stated cons of the traditional diculture and nitrogen fertilizer. This analysis focus on the overview as well as the pro and cons of the genetically modified nitrogen-fixing plants in providing a better agricultural method. The genetically modifying method to generate a nitrogen-fixing non-legumes carries a significant chance of failure results and hindrance. The multilevel implication occurs when we need to modify the plants that not normally produce nodules in their roots to form the nodules and to modify the Nitrogen-fixing microbes to live in the nodules of non-legumes, which are not their natural dwelling places.In conclusion, the genetically modified crops project to fix their Nitrogen is feasible, but the difficulties and the funds needed still outweigh the benefits obtained in the future. With all of those limitations, the target goal to erase famine in 2050 just by funding the nitrogen-fixing wheat alone seems to be too high to be reached. The funds and efforts should be better spent on other factors and farming methods

Prevalence and Distribution of Dengue Virus in <i>Aedes aegypti </i>in Yogyakarta City before Deployment of Wolbachia Infected <i>Aedes aegypti</i>

Indonesia is one of the countries where dengue infection is prevalent. In this study we measure the prevalence and distribution of dengue virus (DENV) DENV-infected Aedes aegypti in Yogyakarta City, Indonesia, during the wet season when high dengue transmission period occurred, as baseline data before implementation of a Wolbachia-infected Aedes aegypti trial for dengue control. We applied One-Step Multiplex Real Time PCR (RT-PCR) for the type-specific-detection of dengue viruses in field-caught adult Aedes aegypti mosquitoes. In a prospective field study conducted from December 2015 to May 2016, adult female Aedes aegypti were caught from selected areas in Yogyakarta City, and then screened by using RT-PCR. During the survey period, 36 (0.12%) mosquitoes from amongst 29,252 female mosquitoes were positive for a DENV type. In total, 22.20% of dengue-positive mosquitoes were DENV-1, 25% were DENV-2, 17% were DENV-3, but none were positive for DENV-4. This study has provided dengue virus infection prevalence in field-caught Aedes aegypti and its circulating serotype in Yogyakarta City before deployment of Wolbachia-infected Aedes aegypti

Wolbachia genetic similarity in different insect host species: Drosophila melanogaster and Yogyakarta’s (Indonesia) Aedes aegypti as a novel host

Aedes aegypti as a novel host. Biodiversitas 23: 2321-2328. Wolbachia naturally presents in a large number of insects and other arthropod species. The Wolbachia strain wMel from Drosophila melanogaster has been stably transinfected into Aedes aegypti where it stops the mosquito host from being infected with medically important arbovirus like dengue. Consequently, Ae. aegypti infected with wMel have been released in Indonesia as a public health intervention against dengue. This study genetically compared wMel from Yogya field-caught D. melanogaster and the wMel in stably transfected Ae. aegypti used for field releases in Yogyakarta, Indonesia. The genetic similarity between wMel Wolbachia was evaluated by sequencing of Wolbachia surface protein (wsp) gene and some polymorphic genomic regions of insertion sites (IS) and variable number tandem repeats (VNTR) loci. The sequence of the Wolbachia surface protein (wsp) gene was 100% identical between hosts. There is no insertion sequence among specimens. The insertion sequence IS-WD1310 was identical between wMel from both hosts and among other strains, as well as the IS-WD516/7. The VNTR-141 period was identical within wMel from both hosts and among other strains, the VNTR-105 as well. Wolbachia Yogya field-caught D. melanogaster and Wolbachia strain wMel present in Ae. aegypti used for bio-control of dengue were genetically identical. These findings provide beneficial understanding to answer the public attention on safety issues, especially on the genetic similarity between Wolbachia strain in the natural and transfected hosts of this novel technology for dengue control