56 research outputs found
Statistical correlation between enterovirus genome copy numbers and infectious viral particles in wastewater samples
Aims: Classic virological tests are time consuming and labour-intensive; realtime
RT-PCR has proven to be a fast method to detect and quantify enterovirus
genomes in clinical and environmental samples. This method is unable to
discriminate between infective and noninfective enterovirus particles; few clinical
studies have compared real-time RT-PCR and viral culture. We wondered if
the enterovirus genome quantification could be correlated to the infectivity.
Methods and Results: We used the statistical approach to verify our hypotheses
to correlate data, obtained by the standard method (most probable number of
cytopathic units—MPNCU) and molecular test (real-time RT-PCR), on wastewater
treatment plant samples. Chi-squared test was used, considering several
cut-off values (‘50’-‘100’-‘200’ genome copy numbers), to determine statistical
significance in comparison of the two methods. Chi-square value was not significant
when cut-off of 50 (P = 0Æ103) and 100 (P = 0Æ178) was assumed but
was significant with cut-off of 200 (P = 0Æ044).
Conclusion: This limit, 200 genome copy, could be used as cut-off value to
indicate enterovirus survival in environmental monitoring.
Significant and Impact of the Study: To introduce a fast procedure that is able
to compensate for disadvantages of cell culture method for viral environmental
analyses
Electrochemical oxidation of water on synthetic boron-doped diamond thin film anodes
Electrolysis in aqueous 1 M HClO4 and 1 M H2SO4 solutions has been carried out under galvanostatic conditions using boron-doped diamond electrodes (BDD). Analyses of the oxidation products have shown that in 1 M HClO4 the main reaction is oxygen evolution, while in H2SO4 the main reaction is the formation of H2S2O8. In both electrolytes small amounts of O3 and H2O2 are formed. Finally, a simplified mechanism involving hydroxyl radicals formed by water discharge has been proposed for water oxidation on boron-doped diamond anode
Laparoscopic diverting colostomy in the therapeutic management of large bowel obstructions in neoplastic elderly patients
ICAROS (Italian survey on CardiAc RehabilitatiOn and Secondary prevention after cardiac revascularization): Temporary report of the first prospective, longitudinal registry of the cardiac rehabilitation network GICR/IACPR
Electrochemical oxidation of Cu-EDTA on boron doped diamond (BDD) electrode
No Abstract. Global Journal of Pure and Applied Sciences Vol. 12 (4) 2006: 577-58
Investigation of Trace and Ultra-Trace Elements of Nutritional and Toxicological Significance in Italian Potable Waters by INAA
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