Infectious agents in atherosclerotic cardiovascular diseases through oxidative stress

Accumulating evidence demonstrates that vascular oxidative stress is a critical feature of atherosclerotic process, potentially triggered by several infectious agents that are considered as risk co-factors for the atherosclerotic cardiovascular diseases (CVDs). C. pneumoniae has been shown to upregulate multiple enzymatic systems capable of producing reactive oxygen species (ROS) such as NADPH oxidase (NOX) and cyclooxygenase in vascular endothelial cells, NOX and cytochrome c oxidase in macrophages as well as nitric oxide synthase and lipoxygenase in platelets contributing to both early and late stages of atherosclerosis. P. gingivalis seems to be markedly involved in the atherosclerotic process as compared to A. actinomycetemcomitans contributing to LDL oxidation and foam cell formation. Particularly interesting is the evidence describing the NLRP3 inflammasome activation as a new molecular mechanism underlying P. gingivalis-induced oxidative stress and inflammation. Amongst viral agents, immunodeficiency virus-1 and hepatitis C virus seem to have a major role in promoting ROS production, contributing, hence, to the early stages of atherosclerosis including endothelial dysfunction and LDL oxidation. In conclusion, oxidative mechanisms activated by several infectious agents during the atherosclerotic process underlying CVDs are very complex and not well-known, remaining, thus, an attractive target for future research

Nomenclature adjustments and new syntaxa of the arctic, alpine and oro-Mediterranean vegetation

Proposte sintassonomiche e nomenclaturali per la vegetazione della Tundra alpina. Il capitolo riguardante l'alta quota appenninica è il più corposo e propone il nuovo syntaxon Leontopodio-Elynion a livello di alleanza. La grande novità sta nel fatto che questa alleanza è da considerarsi anfi-Adriatica e unsice Appennino e balcani, differenziandosi dall'alleanza già definita da altri per le Alpi. i Pirenei e i Carpazi.During preparation of the European checklist of vegetation units (EuroVegChecklist), it became clear that some earlier described syntaxa need to be typified in order to stabilize nomenclature and some new syntaxa need to be described. Here we propose nomenclature adjustments and formal description of four new alliances for the Arctic, alpine and oro-Mediterranean vegetation of Europe, Greenland and Anatolia. First, we typify the class Juncetea trifidi. Second, we describe four new alliances, such as the Puccinellion nuttallianae (Low-Arctic salt steppes of Greenland; class Saxifrago tricuspidatae-Calamagrostietea purpurascentis), Dryado octopetalae- Caricion arctisibiricae (Arctic tundra vegetation of north-eastern European Russia; class Carici rupestris-Kobresietea bellardii), Leontopodio nivalis-Elynion myosuroidis (southern European alpine tundra vegetation; class Carici rupestris-Kobresietea bellardii) and Lagotido uralensis-Caricion ensifoliae (alpine tundra vegetation of the Southern Ural Mountains; class Juncetea trifidi). Two new associations are described within the first two of these alliances. Finally, we present an interpretation of the alliance Muscario-Scillion nivalis

Liquidity Creation and Bank Capital

This paper aims to evaluate the relationship between capital and liquidity following the implementation of the Basel III rules. These regulatory measures target both increased capital ratios and a reduction of banks’ maturity transformation risk , which could result in excessive constraints on bank liquidity creation, thereby negatively affecting economic growth. Using a simultaneous equation model, we find a bi-causal negative relationship, which suggests that banks may reduce liquidity creation as capital increases; and when liquidity creation increases, banks reduce capital ratios. Our results therefore imply a trade-off between financial stability (higher capital, reduced risk) and economic growth (liquidity creation)

The regions of the sequence most exposed to the solvent within the amyloidogenic state of a protein initiate the aggregation process.

Formation of misfolded aggregates is an essential part of what proteins can do. The process of protein aggregation is central to many human diseases and any aggregating event needs to be prevented within a cell and in protein design. In order to aggregate, a protein needs to unfold its native state, at least partially. The conformational state that is prone to aggregate is difficult to study, due to its aggregating potential and heterogeneous nature. Here, we use a systematic approach of limited proteolysis, in combination with electrospray ionisation mass spectrometry, to investigate the regions that are most flexible and solvent-exposed within the native, ligand-bound and amyloidogenic states of muscle acylphosphatase (AcP), a protein previously shown to form amyloid fibrils in the presence of trifluoroethanol. Seven proteases with different degrees of specificity have been used for this purpose. Following exposure to the aggregating conditions, a number of sites along the sequence of AcP become susceptible to proteolytic digestion. The pattern of proteolytic cleavages obtained under these conditions is considerably different from that of the native and ligand-bound conformations and includes a portion within the N-terminal tail of the protein (residues 6-7), the region of the sequence 18-23 and the position 94 near the C terminus. There is a significant overlap between the regions of the sequence found to be solvent-exposed from the present study and those previously identified to be critical in the rate-determining steps of aggregation from protein engineering approaches. This indicates that a considerable degree of solvent exposure is a feature of the portions of a protein that initiate the process of aggregation

Multiple ectopic leiomyomas of the abdominal rectus muscles after gasless laparoscopic uterine myomectomy

To describe and analyze the first case of multiple ectopic leiomyomas of the abdominal rectus muscles in a patient who had undergone gasless laparoscopic uterine myomectomy (GLM) 10 years before

The molecular chaperone Hsp90 is a component of the cap-binding complex and interacts with the translational repressor Cup during Drosophila oogenesis

In metazoa, the spatio-temporal translation of diverse mRNAs is essential to guarantee proper oocyte maturation and early embryogenesis. The eukaryotic translation initiation factor 4E (eIF4E), which binds the 5′ cap structure of eukaryotic mRNAs, associates with either stimulatory or inhibitory factors to modulate protein synthesis. In order to identify novel factors that might act at the translational level during Drosophila oogenesis, we have undertaken a functional proteomic approach and isolated the product of the Hsp83 gene, the evolutionarily conserved chaperone Hsp90, as a specific component of the cap-binding complex. Here we report that Hsp90 interacts in vitro with the translational repressor Cup. In addition, we show that Hsp83 and cup interact genetically, since lowering Hsp90 activity enhances the oogenesis alterations linked to diverse cup mutant alleles. Hsp90 and Cup co-localize in the cytoplasm of the developing germ-line cells within the germarium, thus suggesting a common function from the earliest stages of oogenesis. Taken together, our data start elucidating the role of Hsp90 during Drosophila female germ-line development and strengthen the idea that Cup has multiple essential functions during egg chamber development

The academic impact of natural disasters: evidence from L’Aquila earthquake

This paper uses a standard difference-in-differences approach to examine the effect of the L’Aquila earthquake on the academic performance of the students of the local university. The empirical results indicate that this natural disaster reduced students’ probability of graduating on-time and slightly increased students’ probability of dropping out. While post-disaster measures (e.g. fast re-establishment of education activities in temporary locations) are likely to have mitigated the effects of this event, disruptions in the learning environment and the mental trauma suffered by students in the aftermath of the earthquake may have worsened their academic performance

HyBIS: Windows Guest Protection through Advanced Memory Introspection

Effectively protecting the Windows OS is a challenging task, since most implementation details are not publicly known. Windows has always been the main target of malwares that have exploited numerous bugs and vulnerabilities. Recent trusted boot and additional integrity checks have rendered the Windows OS less vulnerable to kernel-level rootkits. Nevertheless, guest Windows Virtual Machines are becoming an increasingly interesting attack target. In this work we introduce and analyze a novel Hypervisor-Based Introspection System (HyBIS) we developed for protecting Windows OSes from malware and rootkits. The HyBIS architecture is motivated and detailed, while targeted experimental results show its effectiveness. Comparison with related work highlights main HyBIS advantages such as: effective semantic introspection, support for 64-bit architectures and for latest Windows (8.x and 10), advanced malware disabling capabilities. We believe the research effort reported here will pave the way to further advances in the security of Windows OSes

New insights into Chlamydiae persistence: an energy metabolism strategy?

Chlamydiaceae is a family of obligate intracellular bacteria generally considered energy parasites. Several studies have suggested that Chlamydiae are capable of independently producing energy and, more importantly, several genes involved in the energy metabolism are up-regulated during the persistent state. Thus, it has been suggested that chlamydial persistence could be a complex and flexible metabolic strategy designed to favor a lengthy survival in the host cell by evading the immune response. In conclusion, more detailed studies on the shift in the chlamydial energy metabolism, from the active to the persistent form, may be helpful in future to determine whether chlamydial persistence observed in vitro does occur in vivo and whether chronic sequelae of chlamydial diseases may be related to the persistence

Differential response to hepatic differentiation stimuli of amniotic epithelial cells isolated from four regions of the amniotic membrane

Human Amniotic Epithelial Cells (hAEC) isolated from term placenta are a promising source for regenerative medicine. However, it has long been debated whether the hAEC population consists of heterogeneous or homogeneous cells. In a previous study, we investigated the characteristics of hAEC isolated from four different regions of the amniotic membrane finding significant heterogeneity. The aim of this study was to evaluate the hepatic differentiation capability of hAEC isolated from these four regions. Human term placentae were collected after caesarean section and hAEC were isolated from four regions of the amniotic membrane (R1-R4, according to their relative distance from the umbilical cord) and treated in hepatic differentiation conditions for 14 days. hAEC-derived hepatocyte-like cells showed marked differences in the expression of hepatic markers: R4 showed higher levels of Albumin and Hepatocyte Nuclear Factor (HNF) 4α whereas R1 expressed higher Cytochrome P450 enzymes, both at the gene and protein level. These preliminary results suggest that hAEC isolated from R1 and R4 of the amniotic membrane are more prone to hepatic differentiation. Therefore, the use of hAEC from a specific region of the amniotic membrane should be taken into consideration as it could have an impact on the outcome of therapeutic applications