Promjena nekih biokemijskih vrijednosti u Honamli koza i dugodlakih koza u vrijeme spolnog sazrijevanja.

Honamlı goats have been defined and recorded as a new breed but the literature is very limited on these goats. In terms of meat production, they have been reported as having some of the highest potential among the goat breeds in Turkey. The aim of the present study was to determine the alterations in blood serum chemistry values in the puberty period in Honamlı and Native Hair goats. Selected biochemistry parameters were chosen due to their association with meat production potential. Blood samples of Honamlı (n = 90, 45 animals for each sex) and Native Hair goats (n = 90, 45 animals for each sex) were obtained from goat herds in the Western Mediterranean region of Turkey. The values of alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate transaminase (AST), creatine kinase (CK), calcium, cholesterol, creatinine, iron, magnesium, phosphorus, total lipids, triglycerides and uric acid were measured in the sera obtained from blood samples of males and females in equal numbers of each species, in the three age groups of 4, 8 and 12 months. One-way analysis of variance and the Tukey test were performed for statistical evaluation. In both male and female animals, ALT, AST, CK, calcium, cholesterol, creatinine, iron, magnesium, phosphorus, total lipids, triglycerides and uric acid levels exhibited significant (P<0.05) differences between the age groups. Our findings suggest that there are age-associated parallel increases of ALT values in female goats and total lipid values in male goats. The present study also reveals that the analyzed biochemical values usually change after 4 months of age when compared with the other age groups.Honamli koza je nova pasmina koza pa su literaturni podatci o njoj vrlo oskudni. Jedna je od pasmina s najvećim mogućnostima za proizvodnju mesa u Turskoj. Cilj je ovog rada odrediti promjene biokemijskih vrijednosti u krvnom serumu Honamli koza i dugodlakih koza za vrijeme spolnog sazrijevanja. Za istraživanje su odabrani neki biokemijski pokazatelji povezani s proizvodnjom mesa. Uzorci krvi Honamli koza (n = 90, po 45 životinja oba spola) i dugodlakih koza (n = 90, po 45 životinja oba spola) uzeti su od stada u Zapadnom Mediteranskom području Turske. Vrijednosti alkalne fosfataze (ALP), alanin transaminaze (ALT), aspartat transaminaze (AST), kreatin kinaze (CK), kalcija, kolesterola, kreatinina, željeza, magnezija, fosfora, ukupnih lipida, triglicerida i mokraćne kiseline izmjerene su u uzorcima seruma uzetima od muških i ženskih životinja obiju pasmina podijeljenih u skupine prema dobi od 4, 8 i 12 mjeseci. Jednostavna analiza varijance i Tukeyev test rabljeni su za statističku obradbu podataka. I u muških i u ženskih životinja ustanovljene su značajne razlike (P<0,05) među dobnim skupinama za razine ALT, AST, CK, kalcija, kolesterola, kreatinina, željeza, magnezija, fosfora, ukupnih lipida, triglicerida i mokraćne kiseline. Nalazi pokazuju usporedno povećanje vrijednosti ALT u ženskih životinja i vrijednosti ukupnih lipida u muških životinja. Analizirane biokemijske vrijednosti obično se mijenjaju nakon 4 mjeseca starosti u usporedbi s drugim dobnim skupinama

Učinci liječenja fenetil esterom kavene kiseline na reumatološke pokazatelje i metabolizam željeza u štakorica s izazvanom šećernom bolešću.

Diabetology and rheumatology have much in common, including many biochemical features. Diabetic cases suffer excessively from common musculoskeletal conditions. The present study aimed to explore the association between the caffeic acid phenethyl ester (CAPE) treatment and the standard parameters of diabetic and rheumatologic controls. Serum anti-streptolysin-O (ASO), C-reactive protein (CRP), rheumatoid factor (RF) titers and glucose (GLU), cholesterol (CHOL), triglyceride (TG), high density lipoprotein (HDL), very low density lipoprotein (VLDL), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), sodium (Na), potassium (K), chloride (Cl), calcium (Ca), phosphorus (P), uric acid (UA), iron (Fe), iron-binding capacity (IBC), total iron-binding capacity (TIBC) and superoxide dismutase (SOD) levels were measured in 22 female Wistar-Albino rats. The animals were divided into three groups: group 1: non-diabetic rats as control; group 2: streptozotocin (STZ)-induced, untreated diabetic rats; and group 3: STZ-induced, CAPE-treated diabetic rats. Levels of GLU were increased in group 2. ASO values were decreased in group 3. TG and VLDL levels were increased in group 3 compared with the group 1. Higher UA levels were detected in group 3 compared with group 2. The levels of Fe and TIBC were decreased in group 3. The results of the present study indicate the reducing effect of CAPE on the serum levels of diabetic and rheumatologic parameters in diabetic rats.Dijabetologija i reumatologija imaju mnoge sličnosti, uključujući biokemijske procese. Oboljeli od dijabetesa pate od teških stanja mišićno-koštanog sustava. U ovom radu istražena je povezanost između liječenja fenetil esterom kavene kiseline i standardnih pokazatelja koji se kontroliraju pri dijabetičnim i reumatoidnim bolestima. Kod 22 Wistar-Albino štakorice mjereni su: serumski anti-streptolizin-O (ASO), C-reaktivni protein (CRP), titar reumatoidnog faktora (RF), zatim razina glukoze (GLU), kolesterola (CHOL), triglicerida (TG), lipoproteina visoke gustoće (HDL), lipoproteina vrlo niske gustoće (VLDL), alkalne fosfataze (ALP), gamaglutamil transferaze (GGT), natrija (Na), kalija (K), klorida (Cl), kalcija (Ca), fosfora (P), mokraćne kiseline (UA), željeza (Fe), kapaciteta vezanja željeza (IBC), ukupnog kapaciteta vezanja željeza (TIBC) i superoksid dismutaze (SOD). Životinje su bile podijeljene u tri skupine: 1. štakorice bez dijabetesa (kontrolna skupina), 2. neliječene štakorice s dijabetesom izazvanim streptozotocinom (STZ) i 3. štakorice sa streptozotocin (STZ) - izazvanim dijabetesom liječenim fenetil esterom kavene kiseline. Razine GLU bile su povećane u skupini 2. ASO vrijednosti bile su snižene u skupini 3. Razine TG i VLDL bile su povišene u skupini 3 u usporedbi s razinama u skupini 1. Više razine UA utvrđene su u skupini 3 u usporedbi sa skupinom 2. Razine Fe i TIBC bile su snižene u skupini 3. Rezultati pokazuju reducirajuće učinke fenetil estera kavene kiseline na razinu dijabetičnih i reumatoloških pokazatelja u serumu štakorica sa šećernom bolešću izazvanom streptozotocinom

The Effects of Periostin in a Rat Model of Isoproterenol: Mediated Cardiotoxicity

Sozmen, Mahmut/0000-0001-7976-4051WOS: 000426954900004PubMed: 28895052Periostin is an extracellular matrix protein from fasciclin family, and it plays an important role in the cell adhesion, migration, and growth of the organism. Periostin prevents apoptosis while stimulating cardiomyocytes. The present study was designed to investigate cardioprotective effects of the recombinant murine periostin peptide administration in a rat model of isoproterenol (ISO)-induced myocardial injury. The experiment was performed on 84 adult male Sprague Dawley rats in 4 groups (n = 21): control group (1), periostin-treated group (2), ISO-treated group (3), and ISO + periostin-treated group (4). The groups were further divided into three subgroups based on the duration of the experiment in which rats were killed on days 1, 7, and 28 (n = 7). Growth factors (VEGF, ANGPT, FGF-2, TGF beta), mitosis and apoptosis (Bcl-2, Bax, PCNA, Ki-67, phospho-histone H3), cell cycle activators and inhibitors (cyclin D1, D2, A2, Cdc2), the origin of regenerating cells (cKit and CD45) mRNA were detected using quantitative real-time polymerase chain reaction (PCR) and PCR array. Immunohistochemistry staining was used to directly detect protein level and distribution in the heart tissues. Administration of periostin following ISO-induced cardiotoxicity revealed that periostin alleviated deleterious effects of ISO through several pathways: (1) periostin induced mitotic activity of endothelial/fibroblastic cells, (2) periostin drives cardiomyocytes into S and M phases, thus promoting proliferation of cardiomyocytes, (3) periostin contributed to collagen degradation, tissue remodeling, and reduced cardiac fibrosis during the healing process following myocardial damage while preserving tissue matrix, (4) periostin stimulated angiogenesis by upregulating THBS1, TGFB2, and HGF genes, (5) periostin regulated cell growth and proliferation while maintaining cell shape and cellular muscle contractions (ACTB) and functioned as chemoattractant factor (CCL2) at the beginning of myocardial damage.Turkish Scientific Research Council (TUBITAK-TOVAG), Ankara, Turkey [114O734]This project was financially supported by Turkish Scientific Research Council (TUBITAK-TOVAG; Project No: 114O734), Ankara, Turkey

Alterations in some clinical biochemistry values of Honamli and Native Hair goats during pubertal development

WOS: 000369517400006Honamli goats have been defined and recorded as a new breed but the literature is very limited on these goats. In terms of meat production, they have been reported as having some of the highest potential among the goat breeds in Turkey. The aim of the present study was to determine the alterations in blood serum chemistry values in the puberty period in Honamli and Native Hair goats. Selected biochemistry parameters were chosen due to their association with meat production potential. Blood samples of Honamli (n = 90, 45 animals for each sex) and Native Hair goats (n = 90, 45 animals for each sex) were obtained from goat herds in the Western Mediterranean region of Turkey. The values of alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate transaminase (AST), creatine kinase (CK), calcium, cholesterol, creatinine, iron, magnesium, phosphorus, total lipids, triglycerides and uric acid were measured in the sera obtained from blood samples of males and females in equal numbers of each species, in the three age groups of 4, 8 and 12 months. One-way analysis of variance and the Tukey test were performed for statistical evaluation. In both male and female animals, ALT, AST, CK, calcium, cholesterol, creatinine, iron, magnesium, phosphorus, total lipids, triglycerides and uric acid levels exhibited significant (P<0.05) differences between the age groups. Our findings suggest that there are age-associated parallel increases of ALT values in female goats and total lipid values in male goats. The present study also reveals that the analyzed biochemical values usually change after 4 months of age when compared with the other age groups.Scientific and Technological Research Council of Turkey (TUBITAK-TOVAG)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [112O939]; TUBITAK-TOVAGTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)This article is a part of the project funded by the Scientific and Technological Research Council of Turkey (TUBITAK-TOVAG; Project no.: 112O939). Authors' special thanks are extended to TUBITAK-TOVAG for financial support and Scientific and Technology Application and Research Center, Mehmet Akif Ersoy University for providing laboratory facilities

Učinci liječenja fenetil esterom kavene kiseline na reumatološke pokazatelje i metabolizam željeza u štakorica s izazvanom šećernom bolešću.

Diabetology and rheumatology have much in common, including many biochemical features. Diabetic cases suffer excessively from common musculoskeletal conditions. The present study aimed to explore the association between the caffeic acid phenethyl ester (CAPE) treatment and the standard parameters of diabetic and rheumatologic controls. Serum anti-streptolysin-O (ASO), C-reactive protein (CRP), rheumatoid factor (RF) titers and glucose (GLU), cholesterol (CHOL), triglyceride (TG), high density lipoprotein (HDL), very low density lipoprotein (VLDL), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), sodium (Na), potassium (K), chloride (Cl), calcium (Ca), phosphorus (P), uric acid (UA), iron (Fe), iron-binding capacity (IBC), total iron-binding capacity (TIBC) and superoxide dismutase (SOD) levels were measured in 22 female Wistar-Albino rats. The animals were divided into three groups: group 1: non-diabetic rats as control; group 2: streptozotocin (STZ)-induced, untreated diabetic rats; and group 3: STZ-induced, CAPE-treated diabetic rats. Levels of GLU were increased in group 2. ASO values were decreased in group 3. TG and VLDL levels were increased in group 3 compared with the group 1. Higher UA levels were detected in group 3 compared with group 2. The levels of Fe and TIBC were decreased in group 3. The results of the present study indicate the reducing effect of CAPE on the serum levels of diabetic and rheumatologic parameters in diabetic rats.Dijabetologija i reumatologija imaju mnoge sličnosti, uključujući biokemijske procese. Oboljeli od dijabetesa pate od teških stanja mišićno-koštanog sustava. U ovom radu istražena je povezanost između liječenja fenetil esterom kavene kiseline i standardnih pokazatelja koji se kontroliraju pri dijabetičnim i reumatoidnim bolestima. Kod 22 Wistar-Albino štakorice mjereni su: serumski anti-streptolizin-O (ASO), C-reaktivni protein (CRP), titar reumatoidnog faktora (RF), zatim razina glukoze (GLU), kolesterola (CHOL), triglicerida (TG), lipoproteina visoke gustoće (HDL), lipoproteina vrlo niske gustoće (VLDL), alkalne fosfataze (ALP), gamaglutamil transferaze (GGT), natrija (Na), kalija (K), klorida (Cl), kalcija (Ca), fosfora (P), mokraćne kiseline (UA), željeza (Fe), kapaciteta vezanja željeza (IBC), ukupnog kapaciteta vezanja željeza (TIBC) i superoksid dismutaze (SOD). Životinje su bile podijeljene u tri skupine: 1. štakorice bez dijabetesa (kontrolna skupina), 2. neliječene štakorice s dijabetesom izazvanim streptozotocinom (STZ) i 3. štakorice sa streptozotocin (STZ) - izazvanim dijabetesom liječenim fenetil esterom kavene kiseline. Razine GLU bile su povećane u skupini 2. ASO vrijednosti bile su snižene u skupini 3. Razine TG i VLDL bile su povišene u skupini 3 u usporedbi s razinama u skupini 1. Više razine UA utvrđene su u skupini 3 u usporedbi sa skupinom 2. Razine Fe i TIBC bile su snižene u skupini 3. Rezultati pokazuju reducirajuće učinke fenetil estera kavene kiseline na razinu dijabetičnih i reumatoloških pokazatelja u serumu štakorica sa šećernom bolešću izazvanom streptozotocinom

Expression levels of angiogenic growth factors in feline squamous cell carcinoma

GULBAHAR, MUSTAFA YAVUZ/0000-0001-8268-7659WOS:000539096800007PubMed: 32384073Squamous cell carcinoma (SCC) is the most common malignant neoplasm of the skin in cats. Tumour angiogenesis is the pivotal event for tumour progression and metastasis. We assessed protein and gene expression of angiogenic growth factors including bFGF, VEGF-C, TGF-beta, PDGF-A, PDGF-C and PDGFR-alpha that possibly contribute to the angiogenic phenotype of feline SCC (FSCC) and could, therefore, be a good target in the treatment of SCC. In the present study, a total of 27 FSCC cases were investigated. Tumour cases were histopathologically classified as well differentiated (10/27), moderately differentiated (5/27), and poorly differentiated (12/27). The expression levels of the growth factors were detected using immunohistochemistry and assessed semi-quantitatively. Growth factor expression levels were evaluated at different locations: in the oral region, in areas exposed to solar UV radiation including the ears, eyelids and nasal planum, and other miscellaneous locations. Our findings have revealed that FSCC arising from different anatomical sites of the body and showing differences in aggressiveness, metastasis, and prognosis may be angiogenesis dependent, and angiogenic key regulators could play a role in the development of FSCC.Ondokuz Mayis University Scientific Research and Development Support Program, Samsun, TurkeyOndokuz Mayis University [PYO.VET.1901.13.001]This study was financially supported by Ondokuz Mayis University Scientific Research and Development Support Program (Project No.: PYO.VET.1901.13.001), Samsun, Turkey

Expression levels of angiogenic growth factors in feline squamous cell carcinoma

Squamous cell carcinoma (SCC) is the most common malignant neoplasm of the skin in cats. Tumour angiogenesis is the pivotal event for tumour progression and metastasis. We assessed protein and gene expression of angiogenic growth factors including bFGF, VEGF-C, TGF-beta, PDGF-A, PDGF-C and PDGFR-alpha that possibly contribute to the angiogenic phenotype of feline SCC (FSCC) and could, therefore, be a good target in the treatment of SCC. In the present study, a total of 27 FSCC cases were investigated. Tumour cases were histopathologically classified as well differentiated (10/27), moderately differentiated (5/27), and poorly differentiated (12/27). The expression levels of the growth factors were detected using immunohistochemistry and assessed semi-quantitatively. Growth factor expression levels were evaluated at different locations: in the oral region, in areas exposed to solar UV radiation including the ears, eyelids and nasal planum, and other miscellaneous locations. Our findings have revealed that FSCC arising from different anatomical sites of the body and showing differences in aggressiveness, metastasis, and prognosis may be angiogenesis dependent, and angiogenic key regulators could play a role in the development of FSCC