Lymphocyte Subpopulations in Pulmonary Tuberculosis Patients

Protection against Mycobacterium tuberculosis is based on cell-mediated immunity, most importantly involving CD4(+) and CD8(+) T-cell subsets. The aim of this study was to evaluate CD4(+) and CD8(+) T-cell profiles and CD19(+) and CD3(−)CD(16+56)(+) populations in patients with pulmonary tuberculosis. CD4(+) and CD8(+) T cells, B-lymphocytes, and natural killer (NK) cells were evaluated in 75 active (APTB) and 25 inactive (IPTB) pulmonary tuberculosis cases and 20 healthy subjects (HCs). The results were compared at different stages of antituberculosis treatment in the APTB patients and also according to X-ray findings in the newly diagnosed APTB patients. The percentages of CD4(+) T cells were significantly lower (P < .01) and those of CD3(−)CD(16 + 56)(+) cells were significantly higher (P < .01) in APTB patients than in HCs. CD8(+) T cells were significantly decreased (P < .05), and CD3(−)CD(16+56)(+) cells were significantly increased (P < .01), in IPTB patients compared to HCs. The percentages of CD4(+), CD8(+), CD3(−)CD19(+), and CD3(−)CD(16+56)(+) cells showed no differences at different times of the antituberculosis regimen, and different stages of newly diagnosed APTB patients. APTB patients have a reduced percentage of circulating CD4(+) T cells and an increased percentage of NK cells compared with healthy individuals. These cells could play important roles in the immune response to M tuberculosis infection

The frequency of restless legs syndrome in health care workers

Giriş: Bu çalışmanın amacı sağlık çalışanlarında Huzursuz Bacak Sendromu (HBS) sıklığının saptanması ve etkili faktörlerle ilişkisinin araştırılmasıdır. Materyal ve metod: Kesitsel tipte olan bu araştırmaya toplam 266 doktor, hemşire ve ebe alındı. Araştırma grubundan 213 kişiye ulaşıldı. Olguların demografik verilerini, sosyoekonomik faktörlerini, sigara ve alkol kullanma durumlarını, eşlik eden hastalıklarını, uyku davranışlarını belirlemeye, ayrıca HBS tanı kriterlerinin, şiddetinin ve obstrüktif uyku apne sendromunun (OUAS) riskinin belirlenmesine yönelik soruları içeren anket formu karşılıklı görüşme yoluyla uygulandı. Bulgular: Olguların 49’u erkek, 164’ü kadın idi. Elli bir olgu doktor, 162’si ise hemşire ve ebe olarak görev yapmaktaydı. Sağlık çalışanlarında HBS sıklığı %18.3 (n=39) idi. HBS sıklığı açısından kadın ve erkekler arasında istatistiksel fark saptanmadı. HBS saptanan 39 kişinin 23’ünde (%59) aile öyküsü mevcuttu. HBS saptananlarda HBS saptanmayanlara göre işe geç kalma oranı daha yüksek, ortalama uyku süresi daha kısa ve uykuya dalma süresi daha yüksek bulundu. OUAS için yüksek riskli olanların oranı HBS saptananlarda HBS saptanmayanlara göre istatistiksel olarak yüksekti. Lojistik regresyon analizi sonuçlarına göre OUAS açısından yüksek riske sahip olmanın ve eşlik eden hastalık varlığının HBS için bağımsız risk faktörü olduğu saptandı. Sonuç: Genellikle semptomları anksiyete veya strese bağlanan HBS, sorgulandığında sağlık çalışanlarında yüksek oranda tespit edilmiştir. OUAS açısından yüksek riske sahip olmanın HBS için risk faktörü olabilmesi nedeni ile özellikle eşlik eden hastalık varlığında sağlık çalışanlarının başta HBS ve OUAS olmak üzere uyku bozuklukları açısından taranması ve bilinçlendirilmesi gerektiği düşünüldü.Introduction: To investigate the frequency of restless Leg Syndrome (RLS) in healthcare workers and the relation between the effective factors. Material and Method: Totally 266 doctors, nurses, and midwives were included into this cross-sectional study. Two hundred thirteen of study group were reached. The questionnaires about demographic data, socioeconomic factors, smoking, and alcohol status, co-morbidities, sleep behaviours, and diagnostic criteria, and severity of RLS, and the risk of obstructive sleep apnea syndrome (OSAS) were applied by face to face interview. Results: Forty nine of the cases were male, and 164 were female. Fifty one of cases were doctors, 162 were nurses, and midwives. The frequency of RLS was 18.3% in health care workers. The frequency of RLS was not statistically different between genders. Twenty three of 39 cases diagnosed RLS had family history. The rate of being late for work was high, median sleep time was short, and sleep latency was high in RLS diagnosed cases. The ratio of high risk for OSAS was high in RLS diagnosed cases. The results of logistic regression analysis showed that having high risk for OSAS, and presence of co-morbidities were independent risk factors for RLS. Conclusion: The rate of RLS was determined high in health care workers. It is thought that because having high risk for OSAS may be a risk factor for RLS, especially in the presence of co-morbidities, health care workers must query for sleep disorders as RLS and OSAS, and they must be aware of these conditions

Changes in Serum Cytokine Levels in Active Tuberculosis With Treatment

It has been reported that IFN-γ, TNF-α, and IL-12 stimulate, and that IL-10, TGF-β, and IL-4 suppress the protective immune response against tuberculosis. We aim to evaluate changes in the serum levels of pro and antiinflammatory cytokines in active pulmonary tuberculosis (APTB) and the possible effects of treatment on these changes. Serum IL-12p40, IL-4, IL-10, TNF-α, IFN-γ, and TGF-β1 levels were determined in 20 APTB cases (group 1) before and 2, 4, and 6 months after therapy. The same parameters were also determined in 9 inactive pulmonary tuberculosis (IPTB) cases (group 2) and 9 healthy controls (HC, group 3). Before treatment, the mean serum IFN-γ, TNF-α, and IL-10 levels in group 1 were statistically higher than those in group 2 (P=.001, P=.024, P=.016, resp) or group 3 (P=.003, P=.002, P=.011, resp). The levels in group 1 decreased significantly after treatment (P=.001 for IFN-γ, P=.004 for TNF-α, P=.000 for IL-10). The serum levels of IL-12p40 were significantly higher in group 1 than in group 3 (P=.012) and decreased insignificantly after treatment. There was no difference in serum IL-4 and TGF-β1 levels among the groups (P>.05). Because the serum IL-12p40, IL-10, TNF-α, and IFN-γ levels were high in APTB, we believe that these cytokines have important roles in the immune response to Mycobacterium tuberculosis (M tuberculosis). These parameters could be used in follow-up as indicators of the success of APTB therapy

Pulmoner ve ekstrapulmoner tüberküloz hastalarında CCL1 rs159294 T/A gen polimorfizminin araştırılması

PubMed ID: 24298961Investigation of CCL1 rs159294 T/A gene polymorphism in pulmonary and extrapulmonary tuberculosis patients Introduction: The purpose of this study is to reveal whether CCL1 rs159294 T/A polymorphism in pulmonary and extra-pulmonary tuberculosis patients pose a risk to catch tuberculosis or not. Materials and Methods: In the study, peripheral blood samples from the control group, which includes 160 patients, who consulted to Fırat University Faculty of Medicine, Pulmonology Policlinic in Elazı? province and who were diagnosed with tuberculosis; and 160 healthy individuals, were taken and put into tubes containing EDTA. Each tube contained 2 cc blood samples. DNA isolation was made from these blood samples and CCL1 rs159294 T/A polymorphism was defined with PCR-RFLP analysis. Results: For CCL1 rs159294 T/A polymorphism, TT genotype was found in 98 (61.3%) patients, TA genotype was found in 58 (36.3%) patients, AA genotype was found in 4 (2.5%) patients among 160 patients with tuberculosis; and TT genotype was found in 50 (70.4%) patients, TA genotype in 20 (28.2%) patients, AA genotype was found in 1 (1.4%) patient among 71 patients with pulmonary tuberculosis; TT genotype was found in 48 (53.9%) patients TA genotype was found in 38 (42.7%) patients and AA genotype was found in 3 (3.4%) patients among 89 extrapulmonary tuberculosis patients. And in control group, among 160 healthy individuals, TT genotype was found in 100 (62.5%) individuals, TA genotype was found in 58 (36.3%) individuals, AA genotype was found in 2 (1.3%) individuals and no statistically significant difference was found. Conclusion: CCL1 rs159294 T/A polymorphism do not form an inclination to tuberculosis in our population

Investigation of CCL1 rs159294 T/A gene polymorphism in pulmonary and extrapulmonary tuberculosis patients

Giriş: Bu çalışmada amaç, pulmoner ve ekstrapulmoner tüberküloz hastalarındaki, CCL1 rs159294 T/A polimorfizminin tüberküloza yakalanma riski oluşturup oluşturmadığını ortaya koymaktır. Materyal ve Metod: Çalışmamızda Elazığ ili yöresinde Fırat Üniversitesi Tıp Fakültesi Göğüs Hastalıkları Polikliniğine başvuran, tüberküloz teşhisi konulmuş 160 hasta ve 160 sağlıklı bireyden oluşan kontrol grubuna ait kişilerden periferal kan örnekleri alınarak EDTA içeren tüplere 2 cc olacak şekilde konulmuştur. Bu kan örneklerinden DNA izolasyonu gerçekleştirilerek CCL1 rs159294 T/A polimorfizmi PCR-RFLP analizi yapılarak belirlenmiştir. Bulgular: CCL1 rs159294 T/A polimorfizmi için 160 tüberkülozlu hastanın 98 (%61.3)\\\\\\\'inde TT genotipi, 58 (%36.3)\\\\\\\'inde TA genotipi, 4 (%2.5)\\\\\\\'ünde de AA genotipi, 71 pulmoner tüberkülozlu hastanın 50 (%70.4)\\\\\\\'sinde TT genotipi, 20 (%28.2)\\\\\\\'sinde TA genotipi, 1 (%1.4)\\\\\\\'inde de AA genotipi, 89 ekstrapulmoner tüberkülozlu hastanın 48 (%53.9)\\\\\\\'inde TT genotipi, 38 (%42.7)\\\\\\\'inde TA genotipi, 3 (%3.4)\\\\\\\'ünde de AA genotipi tespit edilmiştir. Kontrol grubunda ise 160 sağlıklı bireyin 100 (%62.5)\\\\\\\'ünde TT genotipi, 58 (%36.3)\\\\\\\'inde TA genotipi, 2 (%1.3)\\\\\\\'sinde de AA genotipi belirlenmiş olup, istatistiksel olarak anlamlı bir farklılık saptanamamıştır.Introduction: The purpose of this study is to reveal whether CCL1 rs159294 T/A polymorphism in pulmonary and extrapulmonary tuberculosis patients pose a risk to catch tuberculosis or not. Materials and Methods: In the study, peripheral blood samples from the control group, which includes 160 patients, who consulted to Fırat University Faculty of Medicine, Pulmonology Policlinic in Elazığ province and who were diagnosed with tuberculosis; and 160 healthy individuals, were taken and put into tubes containing EDTA. Each tube contained 2 cc blood samples. DNA isolation was made from these blood samples and CCL1 rs159294 T/A polymorphism was defined with PCR-RFLP analysis. Results: For CCL1 rs159294 T/A polymorphism, TT genotype was found in 98 (61.3%) patients, TA genotype was found in 58 (36.3%) patients, AA genotype was found in 4 (2.5%) patients among 160 patients with tuberculosis; and TT genotype was found in 50 (70.4%) patients, TA genotype in 20 (28.2%) patients, AA genotype was found in 1 (1.4%) patient among 71 patients with pulmonary tuberculosis; TT genotype was found in 48 (53.9%) patients TA genotype was found in 38 (42.7%) patients and AA genotype was found in 3 (3.4%) patients among 89 extrapulmonary tuberculosis patients. And in control group, among 160 healthy individuals, TT genotype was found in 100 (62.5%) individuals, TA genotype was found in 58 (36.3%) individuals, AA genotype was found in 2 (1.3%) individuals and no statistically significant difference was found. Conclusion: CCL1 rs159294 T/A polymorphism do not form an inclination to tuberculosis in our populatio