A Sub-cellular Localization of the Gene Product of the DNase-1 Locus in Drosophila melanogaster

A study was undertaken to determine the subcellular location of DNase-I, a major acid deoxyribonuclease in Drosophila melanogaster. Embryonic tissue used in these experiments was derived from a wild type strain and from a strain homozygous at the DNase-l locus (3-61.8) for the null activity allele, DNase-lnl24. The majority of total acid DNase and DNase-l activity is found in the small particulate fraction of tissue homogenates fractionated by differential centrifugation. The activity exhibits latency in these extracts indicating that it is membrane delimited. DNase-I activity also co-equilibrates in sucrose density gradients with acid phosphatase activity which, in D. melanogaster, is known to be lysosomal. These results suggest that DNase-l is localized largely within the Iysosomes of embryonic tissue. Functional implications of a lysosomal location for DNase-l are discussed

How to Write a Good Technical Paper

The ability to communicate is a critical skill that not only affects our success as professionals, but also determines how effectively lessons from the laboratory and field are transferred into general practice. As members of ACI's Publications Committee, and as authors ourselves, we offer some guidelines on one form of communication: writing technical papers. You can apply the principles we discuss to formal technical reports, reports to clients, conference papers, and journal articles. Publishing organizations prescribe the exact details of form and format. Because a technical paper should reconstruct the process of the investigation, the generic outline of a paper closely resembles the steps of the scientific method: 1. Identification of the problem or question to be investigated; 2. Evaluation of previous work in the field; 3. Formulation of the hypothesis or definition of the key aspects of the investigation; 4. Design of experiments, observations, or both; 5. Gathering of data; 6. Evaluation of data; and 7. Presentation of conclusions

Cellular Origin of Spontaneous Ganglion Cell Spike Activity in Animal Models of Retinitis Pigmentosa

Here we review evidence that loss of photoreceptors due to degenerative retinal disease causes an increase in the rate of spontaneous ganglion spike discharge. Information about persistent spike activity is important since it is expected to add noise to the communication between the eye and the brain and thus impact the design and effective use of retinal prosthetics for restoring visual function in patients blinded by disease. Patch-clamp recordings from identified types of ON and OFF retinal ganglion cells in the adult (36–210 d old) rd1 mouse show that the ongoing oscillatory spike activity in both cell types is driven by strong rhythmic synaptic input from presynaptic neurons that is blocked by CNQX. The recurrent synaptic activity may arise in a negative feedback loop between a bipolar cell and an amacrine cell that exhibits resonant behavior and oscillations in membrane potential when the normal balance between excitation and inhibition is disrupted by the absence of photoreceptor input

PLENARY PANEL: A 360-DEGREE VIEW OF SCIENCE AND AGRICULTURAL POLICY; EDITED TRANSCRIPT

Agricultural and Food Policy,

Nuclear Propelled Vessels and Neutrino Oscillation Experiments

We study the effect of naval nuclear reactors on the study of neutrino oscillations. We find that the presence of naval reactors at unknown locations and times may limit the accuracy of future very long baseline reactor-based neutrino oscillation experiments. At the same time we argue that a nuclear powered surface ship such as a large Russian ice-breaker may provide an ideal source for precision experiments. While the relatively low reactor power would in this case require a larger detector, the source could be conveniently located at essentially any distance from a detector built at an underground location near a shore in a region of the world far away from other nuclear installations. The variable baseline would allow for a precise measurement of backgrounds and greatly reduced systematics from reactor flux and detector efficiency. In addition, once the oscillation measurement is completed, the detector could perform geological neutrino and astrophysical measurements with minimal reactor background.Comment: 4 pages, 2 figure

The Energetic Costs of Cellular Computation

Cells often perform computations in response to environmental cues. A simple example is the classic problem, first considered by Berg and Purcell, of determining the concentration of a chemical ligand in the surrounding media. On general theoretical grounds (Landuer's Principle), it is expected that such computations require cells to consume energy. Here, we explicitly calculate the energetic costs of computing ligand concentration for a simple two-component cellular network that implements a noisy version of the Berg-Purcell strategy. We show that learning about external concentrations necessitates the breaking of detailed balance and consumption of energy, with greater learning requiring more energy. Our calculations suggest that the energetic costs of cellular computation may be an important constraint on networks designed to function in resource poor environments such as the spore germination networks of bacteria.Comment: 9 Pages (including Appendix); 4 Figures; v3 corrects even more typo

Investigation of ultra-high performance concrete under static and blast loads

Conventional concrete works as an important construction material. However, conventional concrete is known to be brittle and prone to tensile failure and cracks. To overcome such defects and improve the dynamic performance of concrete against extreme loading conditions, concrete with different additions and formulae have been developed. In a recent study, to develop ultra-high performance concrete (UHPC) material with better strength and crack control ability, super fine aggregates with high pozzolanic effect were mixed into the steel fibre reinforced concrete instead of the traditional graded coarse aggregates. Furthermore, to achieve high early age strength, nanoscale additives which can accelerate the hydration process of the ordinary Portland cement were also introduced into the concrete composite. A series of uniaxial compression and four-point bending tests had been performed in the laboratory to get the material properties of this innovative concrete material. Great improvement of the concrete uniaxial compressive strength and flexural tensile strength was observed. Field blast tests were carried out on columns made of this UHPC material. Superior blast resistance performance was observed. In the current study, based on the available test data, numerical models are developed and numerical simulations are carried out. The simulation results are found to comply well with the experimental results

Testing the inverted neutrino mass ordering with neutrinoless double-β decay

We quantify the extent to which future experiments will test the existence of neutrinoless double-beta decay mediated by light neutrinos with inverted-ordered masses. While it remains difficult to compare measurements performed with different isotopes, we find that future searches will fully test the inverted ordering scenario, as a global, multi-isotope endeavor. They will also test other possible mechanisms driving the decay, including a large uncharted region of the allowed parameter space assuming that neutrino masses follow the normal ordering