Internal Motility in Stiffening Actin-Myosin Networks

We present a study on filamentous actin solutions containing heavy meromyosin subfragments of myosin II motor molecules. We focus on the viscoelastic phase behavior and internal dynamics of such networks during ATP depletion. Upon simultaneously using micro-rheology and fluorescence microscopy as complementary experimental tools, we find a sol-gel transition accompanied by a sudden onset of directed filament motion. We interpret the sol-gel transition in terms of myosin II enzymology, and suggest a "zipping" mechanism to explain the filament motion in the vicinity of the sol-gel transition.Comment: 4 pages, 3 figure

Multiwavelength campaign on Mrk 509. V. Chandra-LETGS observation of the ionized absorber

We present here the results of a 180 ks Chandra-LETGS observation as part of a large multi-wavelength campaign on Mrk 509. We study the warm absorber in Mrk 509 and use the data from a simultaneous HST-COS observation in order to assess whether the gas responsible for the UV and X-ray absorption are the same. We analyzed the LETGS X-ray spectrum of Mrk 509 using the SPEX fitting package. We detect several absorption features originating in the ionized absorber of the source, along with resolved emission lines and radiative recombination continua. The absorption features belong to ions with, at least, three distinct ionization degrees. The lowest ionized component is slightly redshifted (v = +73 km/s) and is not in pressure equilibrium with the others, and therefore it is not likely part of the outflow, possibly belonging to the interstellar medium of the host galaxy. The other components are outflowing at velocities of -196 and -455 km/s, respectively. The source was observed simultaneously with HST-COS, finding 13 UV kinematic components. At least three of them can be kinematically associated with the observed X-ray components. Based on the HST-COS results and a previous FUSE observation, we find evidence that the UV absorbing gas might be co-located with the X-ray absorbing gas and belong to the same structure.Comment: 12 pages, 7 figures, 9 tables. Accepted for publication in Astronomy & Astrophysic

Multiwavelength campaign on Mrk 509. VI. HST/COS observations of the far-ultraviolet spectrum

We present medium resolution (R~20,000) HST/COS ultraviolet spectra covering 1155-1760 A of the Seyfert 1 Mrk 509 obtained simultaneously with a Chandra/LETGS spectrum as part of a multiwavelength campaign in 2009 that included observations with XMM-Newton, SWIFT, and Integral. Our high S/N spectrum detects additional complexity in the absorption troughs from a variety of sources in Mrk 509, including the outflow from the active nucleus, the ISM and halo of the host galaxy, and infalling clouds or stripped gas from a merger that are illuminated by the AGN. Variability between the STIS and COS observation of the -400 km/s component allows us to set an upper limit on its distance of < 250 pc. Similarly, variability of a component at +150 km/s between two prior FUSE observations limits its distance to < 1.5 kpc. The UV absorption only partially covers the emission from the AGN nucleus. Covering fractions are lower than those previously seen with STIS, and are comparable to those seen with FUSE. Given the larger apertures of COS and FUSE compared to STIS, we favor scattered light from an extended region near the AGN as the explanation for the partial covering. As observed in prior X-ray and UV spectra, the UV absorption has velocities comparable to the X-ray absorption, but the bulk of the ultraviolet absorption is in a lower ionization state with lower total column density than the gas responsible for the X-ray absorption. We conclude that the outflow from the active nucleus is a multiphase wind.Comment: 21 pages, 9 figures. Accepted for publication in Astronomy & Astrophysics, 26 May 201

Multiwavelength campaign on Mrk 509. I. Variability and spectral energy distribution

(Abridged) Active galactic nuclei show a wealth of interesting physical processes, some of which are poorly understood. We want to address a number of open questions, including the location and physics of the outflow from AGN, the nature of the continuum emission, the geometry and physical state of the X-ray broad emission line region, the Fe-K line complex, the metal abundances of the nucleus and finally the interstellar medium of our own Galaxy. We study one of the best targets for these aims, the Seyfert 1 galaxy Mrk 509 with a multiwavelength campaign using five satellites (XMM-Newton, INTEGRAL, Chandra, HST and Swift) and two ground-based facilities (WHT and PAIRITEL). Our observations cover more than five decades in frequency, from 2 um to 200 keV. The combination of high-resolution spectroscopy and time variability allows us to disentangle and study the different components. Our campaign covers 100 days from September to December 2009, and is centred on a simultaneous set of deep XMM-Newton and INTEGRAL observations with regular time intervals, spanning seven weeks. We obtain a continuous light curve in the X-ray and UV band, showing a strong, up to 60% flux increase in the soft X-ray band during the three weeks in the middle of our deepest monitoring campaign, and which is correlated with an enhancement of the UV flux. This allows us to study the time evolution of the continuum and the outflow. By stacking the observations, we have also obtained one of the best X-ray and UV spectra of a Seyfert galaxy ever obtained. In this paper we also study the effects of the spectral energy distribution (SED) that we obtained on the photo-ionisation equilibrium. Thanks to our broad-band coverage, uncertainties on the SED do not strongly affect the determination of this equilibrium.Comment: 10 pages, 5 figures, 2 tables. Accepted for publication in Astronomy & Astrophysic

Опухоли с невыявленным первичным очагом: современные подходы к лечению

Представлены современные методы и схемы лечения разных видов рака с невыясненным очагом и получаемые результаты.Contemporary methods of treatment of various types of cancer with unrevealed focus as well as the obtained results are described

Multiwavelength campaign on Mrk 509 VIII. Location of the X-ray absorber

The bright Seyfert 1 galaxy Mrk 509 was monitored by XMM-Newton and other satellites in 2009 to constrain the location of the outflow. We have studied the response of the photoionised gas to changes in the ionising flux produced by the central regions. We used the 5 discrete ionisation components A-E detected in the time-averaged spectrum taken with the RGS. Using the ratio of fluxed EPIC and RGS spectra, we put tight constraints on the variability of the absorbers. Monitoring with the Swift satellite started 6 weeks before the XMM-Newton observations, allowing to use the ionising flux history and to develop a model for the time-dependent photoionisation. Components A and B are too weak for variability studies, but the distance for component A is known from optical imaging of the [O III] line to be ~3 kpc. During the 5 weeks of the XMM-Newton observations we found no evidence of changes in the 3 X-ray dominant ionisation components C-E, despite a huge soft X-ray intensity increase of 60% in the middle of our campaign. This excludes high-density gas close to the black hole. Instead, using our time-dependent modelling, we find low density and derive firm lower limits to the distance of these components. Component D shows evidence for variability on longer time scales, yielding an upper limit to the distance. For component E we derive an upper limit to the distance based on the argument that the thickness of the absorbing layer must be less than its distance to the black hole. Combining these results, at the 90% confidence level, component C has a distance of >70 pc, component D between 5-33 pc, and component E >5 pc but smaller than 21-400 pc, depending upon modelling details. These results are consistent with the upper limits from the HST/COS observations of our campaign and point to an origin of the dominant, slow (v<1000 km/s) outflow components in the NLR or torus-region of Mrk 509.Comment: 17 pages, 16 figures, accepted for publication in Astronomy and Astrophysic

Multiwavelength campaign on Mrk 509. II. Analysis of high-quality Reflection Grating Spectrometer spectra

We study the bright Seyfert 1 galaxy Mrk~509 with the Reflection Grating Spectrometers (RGS) of XMM-Newton using the RGS multi-pointing mode of XMM-Newton for the first time in order to constrain the properties of the outflow in this object. We want to obtain the most accurate spectral properties from the 600 ks spectrum of Mrk 509 which has excellent statistical quality. We derive an accurate relative calibration for the effective area of the RGS, derive an accurate absolute wavelength calibration, improve the method for adding time-dependent spectra and enhance the efficiency of the spectral fitting by two orders of magnitude. We show the major improvement of the spectral data quality due to the use of the new RGS multi-pointing mode of XMM-Newton. We illustrate the gain in accuracy by showing that with the improved wavelength calibration the two velocity troughs observed in UV spectra are resolved.Comment: 16 pages, 13 figures, 13 tables. Submitted to Astronomy & Astrophysics, revised version after second referee's comment