High-level integration of murine intestinal transcriptomics data highlights the importance of the complement system in mucosal homeostasis.

BACKGROUND: The mammalian intestine is a complex biological system that exhibits functional plasticity in its response to diverse stimuli to maintain homeostasis. To improve our understanding of this plasticity, we performed a high-level data integration of 14 whole-genome transcriptomics datasets from samples of intestinal mouse mucosa. We used the tool Centrality based Pathway Analysis (CePa), along with information from the Reactome database. RESULTS: The results show an integrated response of the mouse intestinal mucosa to challenges with agents introduced orally that were expected to perturb homeostasis. We observed that a common set of pathways respond to different stimuli, of which the most reactive was the Regulation of Complement Cascade pathway. Altered expression of the Regulation of Complement Cascade pathway was verified in mouse organoids challenged with different stimuli in vitro. CONCLUSIONS: Results of the integrated transcriptomics analysis and data driven experiment suggest an important role of epithelial production of complement and host complement defence factors in the maintenance of homeostasis

Cholera in Oceania

For approximately 200 years, cholera has been feared globally as a disease that can cause rapid-onset epidemics. The causative organisms, Vibrio cholerae O1 and O139 serogroups, are endemic to Southern Asia, but appear to spread globally in waves resulting in seven recognised pandemics to date. The current seventh pandemic has seen the introduction of V. cholerae O1 El Tor into the Oceania region. Since 1962 there have been five large outbreaks at a frequency of approximately one per decade. There have also been regular small outbreaks and clusters of disease throughout the region during the seventh pandemic. The most recent outbreak of cholera in the region occurred in Papua New Guinea in 2009–2011, and this was the largest outbreak to occur in the region to date. In Oceania the majority of people live in high-income settings (Australia and New Zealand) so the risk of cholera transmission is low. Despite this, an estimated 6.5 million people living in the region are at risk of cholera. The most important risk factors are inadequate access to safe water and lack of appropriate sanitation and hygiene measures. However, many other factors may contribute to cholera transmission risk, and people living in Pacific Island countries may be at increased risk in the future due to climate change. Strengthening health delivery services in the region will ensure countries are better equipped to handle future cholera outbreaks; and further understanding the epidemiology of cholera and the causative agent in the region could help prevent future transmission

Postharvest agriculture in changing climates: Its importance to African smallholder farmers

Climate change and variability affect not only the field stages and yields of crops, but also what happens to them after harvest. There has been little discussion of the impacts of climate change on postharvest agriculture, and still less on these impacts in developing countries. Many studies have focused on potential crop yield and pre-harvest implications of different climatic projections, but have omitted an analysis of the need and ability to then protect this increasingly valuable harvest as a vital aspect of food security. Postharvest systems will be affected by changes in temperature, rainfall, humidity, extreme events and the natural and human responses to climate change and variability. This study describes typical grain postharvest systems in east and southern Africa and discusses the likely impacts of different climate change trends on postharvest activities, assets and human well-being outcomes. Adaptation opportunities for creating more climate resilient postharvest agricultural systems and associated livelihoods are identified. Many of these adaptation opportunities are already known and understood by postharvest service providers, highlighting the significant challenge of getting postharvest knowledge into use at a larger scale. A discussion is presented on the factors influencing attempts to strengthen the adaptive capacity of postharvest systems, such as its invisibility, its omission from training curricula, innovation system challenges, the policy bias towards pre-harvest agricultural spending, limited understanding of gender and diversity aspects of postharvest roles, and the dominance of maize in the food system. The study recognises the crucial role of postharvest agriculture in helping communities adapt and cope with change