Breeding Sorghum [Sorghum bicolor (L.) Moench] for drought tolerance and medium-maturity.

Doctor of Philosophy in Plant Breeding. University of KwaZulu-Natal, Pietermaritzburg, 2017.Abstract available in PDF file

A new isoflavone from stem bark of Millettia dura

A new isoflavone (7,3’-dimethoxy-4’,5’-methylenedioxyisoflavone) and three known isoflavones [isoerythrinin A 4’-(3-methylbut-2-enyl) ether, isojamaicin and nordurlettone] were isolated from the stem bark of Millettia dura (Leguminosae). The structures were determined by spectroscopic methods. KEY WORDS: Millettia dura, Leguminosae, Isoflavone, 7,3’-Dimethoxy-4’,5’-methylenedioxyisoflavone, Isoerythrinin A 4’-(3-methylbut-2-enyl) ether, Isojamaicin,Nordurlettone_Bull. Chem. Soc. Ethiop. 2003, 17(1), 113-115

Herbal Product Processing Practices of Traditional Medicine Practitioners in Kenya- Key Informant Interviews

Introduction: Herbalists in Kenya use self-taught processing practices which are inadequate. The objective of this study was to conduct an assessment of selected practices used by herbalists during drug processing and to identify knowledge gaps. Method: Four long practicing traditional medicinal practitioners were identified using purposive sampling. An interview guide and field visits were used to gather data. Data analysis was done using content thematic approach. Results: Sources of herbal knowledge were varied with the use of internet being a key finding. Regulatory compliance presented various challenges to the herbalists. The wild and cultivation of herbs were identified as key medicinal sources although the protection of biodiversity was a key concern of the herbalists. The facilities, area of practice and general hygiene were inadequate. Positive and negative practices were identified in processing of the herbal medicines. Conclusions: Secrecy by the herbalists has resulted in limited in innovation. More training of herbalists is required to improve on the quality of their drugs.  It is however encouraging that they have adopted some modern methods in their practice. Keywords: Herbalists, processing practices, herbal drug

Management of Type 2 Diabetes Mellitus by Traditional Medicine Practitioners in Kenya- Key Informant Interviews

Introduction: Worldwide, plant based medicines are increasing in popularity due to perceptions of safety and efficacy. Herbalists in Kenya are widely consulted for the management of many diseases including Type 2 Diabetes Mellitus (T2DM). This study investigated the level of knowledge of the herbalists in management of T2DM. Methods: Purposive sampling was used to identify 4 herbalists working in the urban areas who actively manage T2DM. Key informant interviews were used to gather data about the management of T2DM. It was analyzed using a content thematic approach. Results: Diverse management methods which included both pharmacological and non- pharmacological were noted. Glycemic control was assessed with the help of a glucometer. In addition, presenting signs and symptoms were key in diagnosing T2DM. The herbalists used various herbs, minerals and animals as medicinal sources. The drugs were dispensed as decoctions with excipients being added appropriately. Adverse effects were recorded. The herbalists acknowledged that patients use both herbal and allopathic medicine together. A level of record keeping was observed but patient follow-up was poor. The cost of the herbal drugs was perceived to be excessive. Conclusion: Some similarities exist in the management of T2DM between allopathic and traditional medicine practitioners. Training of herbalists is required to improve the quality of care given to patients.Pan African Medical Journal 2015; 2

Solar Driven Photocatalytic Activity of Porphyrin Sensitized TiO2: Experimental and Computational Studies

The absence of a secure long-term sustainable energy supply is recognized as a major worldwide technological challenge. The generation of H2 through photocatalysis is an environmentally friendly alternative that can help solve the energy problem. Thus, the development of semiconductor materials that can absorb solar light is an attractive approach. TiO2 has a wide bandgap that suffers from no activity in the visible spectrum, limiting its use of solar radiation. In this research, the semiconductor absorption profile was extended into the visible region of the solar spectrum by preparing porphyrin-TiO2 (P-TiO2) composites of meso-tetra(4-bromophenyl)porphyrin (PP1) and meso-tetra(5-bromo-2-thienyl)porphyrin (PP2) and their In(III), Zn(II) and Ga(III) metal complexes. Density functional theory (DFT) and time-dependent density functional theory (TD-DFT) calculations were performed on the porphyrins to gain insight into their electron injection capability. The results demonstrate that P-TiO2 systems merit further in-depth study for applications that require efficient photocatalytic H2 generation

Molecular diagnosis of infectious diseases using cytological specimens

Pathologists have an important role in the diagnosis of infectious disease (ID). In many cases, a definitive diagnosis can be made using cytopathology alone. However, several ancillary techniques can be used on cytological material to reach a specific diagnosis by identifying the causative agent and consequently defining the management of the patient. This review aims to present the effectiveness of the application of molecular studies on cytological material to diagnose IDs and discuss the advantages and disadvantages of the various molecular techniques according to the type of cytological specimen and the infectious agents.info:eu-repo/semantics/publishedVersio

Agro-morphological characterisation and selection of sorghum landraces

Sorghum [Sorghum bicolor (L.) Moench] grown under rain-fed conditions is frequently affected by drought stress at different stages, resulting in reduced grain and biomass yield. The aim of this study was to characterise sorghum landraces and to select farmer-preferred medium-maturing genotypes under rainfed and irrigated conditions. Hundred and ninety-six sorghum accessions were evaluated at Kobo site of the Sirinka Agricultural Research Center in 2014/2015 in Ethiopia. Data collected from 14 traits were subjected to analysis of variance, cluster analysis, Pearson’s correlation coefficient analysis, path coefficient analysis and principal component analysis (PCA). Significant genotypic differences (p < 0.05) were observed. Medium-maturing and drought tolerant sorghum genotypes including E-72457, E-72438, E-72435, E-206214, E-72449, E-75460 and E-75458 with superior agronomic performance were recommend for large-scale production or for further breeding. The genotypes evaluated under rain-fed and irrigated conditions were grouped into five and six clusters, respectively, representing varied different heterotic groups. Grain yield had significant and positive correlation with yield-related traits assessed under the two test conditions. Further, path coefficient analysis revealed that days to maturity under rainfed condition and harvest index under irrigated condition had the highest positive direct effects on grain yield, therefore can be targets for direct selection. Overall, there was marked genetic diversity among the tested genotypes. Suitable medium-maturing farmers-preferred accessions selected from the study will be useful for effective breeding for drought tolerance and medium-maturity

The impact of drought on sorghum production, and farmer's varietal and trait preferences, in the north eastern Ethiopia: implications for breeding

Purpose: Sorghum [Sorghum bicolor (L.) Moench] has been cultivated for centuries as a staple food crop for millions of people in sub-Saharan Africa and Asia. In Ethiopia a total of 4.34 million tons of sorghum is being produced per annum, predominantly in the arid and semi-arid areas. However, the yield and quality of sorghum is affected by a wide array of production constraints, notably severe and recurrent drought stress. The aim of this study were to determine the impact of drought on sorghum production and productivity over time and space, and to identify farmers’ trait preferences, production constraints and coping strategies when dealing with drought in north eastern Ethiopia. Materials and methods: Participatory rural appraisal (PRA) was employed in three administrative zones of north eastern Ethiopia. One Woreda from each Zone and two Kebeles from each Woreda were selected on the basis of sorghum area coverage, production, consumption and prior information on the intensity, duration and spatial coverage of drought. In each kebele, 30 respondents were selected for interview and 12 key informants were selected for group discussions and transect walk personal observations. Data collected from 180 respondents was subjected to statistical analysis and the information gathered from 72 key informants through focus group discussions and transect walk observations were used in discussing the results. Results and discussion: The present study found that productivity of sorghum was challenged by recurrent droughts, Striga infestation, insects, birds, diseases, a lack of varieties with farmers-preferred traits and high yield potential, limited policy support, a lack of improved seed system, poor sorghum production practices and application of crop input and poor soil fertility, in a decreasing order of importance. Among the listed sorghum production constraints, severe drought in the post-flowering stage was identified by most interviewed farmers as the leading constraint across the three study zones. Focus group discussions and transect walk observations held in each Kebele revealed that farmers’ had lost numerous valuable local landrace varieties due to extreme drought conditions over the years. A significant number of interviewed farmers preferred to grow high grain and biomass yielder medium-maturing sorghum varieties which can be sown at the normal planting time but which would escape post-flowering drought. Conclusion: Overall, sorghum breeding programme should be directed at developing farmers’ ideal sorghum varieties with high grain and biomass yield, adequate level of drought and Striga tolerance. In addition, development of farmer preferred medium-maturing sorghum varieties suitable for April planting should be strengthen to boost its productivity and to increase varietal adoption rate in the area

Prevalence and antimicrobial susceptibility pattern of Enterococcus species isolated from different clinical samples at Black Lion Specialized Teaching Hospital, Addis Ababa, Ethiopia

Abstract Objective Enterococci which are parts of the normal intestinal flora are opportunistic human pathogens. Their increasing importance is largely due to their resistance to antimicrobials. So the aim this study was to determine the prevalence and antimicrobial pattern of Enterococcus spp. Result From the total of 422 samples processed, 15 Enterococcus species were isolated. In this study, linezolid were the drug of choice for Enterococcus species, which showed 100% sensitive followed by vancomycin 93.3% sensitive. In contrast, highly resistance (80%) was observed for ampicillin followed by doxycycline (73.3%). All of isolated Enterococci were sensitive to linezolid, however, resistance was observed to common antibiotics. The presence of multidrug resistant Enterococci in our study should be considered as an alarm for Enterococcal infections