28,939 research outputs found
Olig2/Plp-positive progenitor cells give rise to Bergmann glia in the cerebellum.
NG2 (nerve/glial antigen2)-expressing cells represent the largest population of postnatal progenitors in the central nervous system and have been classified as oligodendroglial progenitor cells, but the fate and function of these cells remain incompletely characterized. Previous studies have focused on characterizing these progenitors in the postnatal and adult subventricular zone and on analyzing the cellular and physiological properties of these cells in white and gray matter regions in the forebrain. In the present study, we examine the types of neural progeny generated by NG2 progenitors in the cerebellum by employing genetic fate mapping techniques using inducible Cre-Lox systems in vivo with two different mouse lines, the Plp-Cre-ER(T2)/Rosa26-EYFP and Olig2-Cre-ER(T2)/Rosa26-EYFP double-transgenic mice. Our data indicate that Olig2/Plp-positive NG2 cells display multipotential properties, primarily give rise to oligodendroglia but, surprisingly, also generate Bergmann glia, which are specialized glial cells in the cerebellum. The NG2+ cells also give rise to astrocytes, but not neurons. In addition, we show that glutamate signaling is involved in distinct NG2+ cell-fate/differentiation pathways and plays a role in the normal development of Bergmann glia. We also show an increase of cerebellar oligodendroglial lineage cells in response to hypoxic-ischemic injury, but the ability of NG2+ cells to give rise to Bergmann glia and astrocytes remains unchanged. Overall, our study reveals a novel Bergmann glia fate of Olig2/Plp-positive NG2 progenitors, demonstrates the differentiation of these progenitors into various functional glial cell types, and provides significant insights into the fate and function of Olig2/Plp-positive progenitor cells in health and disease
Technological innovations at the onset of the Mid-Pleistocene Climate Transition in high-latitude East Asia
The interplay between Pleistocene climatic variability and hominin adaptations to diverse terrestrial ecosystems is a key topic in human evolutionary studies. Early and Middle Pleistocene environmental change and its relation to hominin behavioural responses has been a subject of great interest in Africa and Europe, though little information is available for other key regions of the Old World, particularly from Eastern Asia. Here we examine key Early Pleistocene sites of the Nihewan Basin, in high-latitude northern China, dating between ∼1.4 to 1.0 million years ago (Ma). We compare stone tool assemblages from three Early Pleistocene sites in the Nihewan Basin, including detailed assessment of stone tool refitting sequences at the ∼1.1 Ma-old site of Cenjiawan. Increased toolmaking skills and technological innovations are evident in the Nihewan Basin at the onset of the Mid-Pleistocene Climate Transition (MPT). Examination of the lithic technology of the Nihewan sites, together with an assessment of other key Palaeolithic sites of China, indicates that toolkits show increasing diversity at the outset of the MPT and in its aftermath. The overall evidence indicates the adaptive flexibility of early hominins to ecosystem changes since the MPT, though regional abandonments are also apparent in high-latitudes, likely owing to cold and oscillating environmental conditions. The view presented here sharply contrasts with traditional arguments that stone tool technologies of China are homogeneous and continuous over the course of the Early Pleistocene.Introduction Results - Stone-tool-knapping skills recorded in the Cenjiawan assemblage - Technological comparisons of the Nihewan Basin assemblages Discussio
Efficient quantum cryptography network without entanglement and quantum memory
An efficient quantum cryptography network protocol is proposed with
d-dimension polarized photons, without resorting to entanglement and quantum
memory. A server on the network, say Alice, provides the service for preparing
and measuring single photons whose initial state are |0>. The users code the
information on the single photons with some unitary operations. For preventing
the untrustworthy server Alice from eavesdropping the quantum lines, a
nonorthogonal-coding technique (decoy-photon technique) is used in the process
that the quantum signal is transmitted between the users. This protocol does
not require the servers and the users to store the quantum state and almost all
of the single photons can be used for carrying the information, which makes it
more convenient for application than others with present technology. We also
discuss the case with a faint laser pulse.Comment: 4 pages, 1 figures. It also presented a way for preparing decoy
photons without a sinigle-photon sourc
De Novo Genome Sequence of "Candidatus Liberibacter solanacearum" from a Single Potato Psyllid in California.
The draft genome sequence of "Candidatus Liberibacter solanacearum" strain RSTM from a potato psyllid (Bactericera cockerelli) in California is reported here. The RSTM strain has a genome size of 1,286,787Â bp, a G+C content of 35.1%, 1,211 predicted open reading frames (ORFs), and 43 RNA genes
Circular quantum secret sharing
A circular quantum secret sharing protocol is proposed, which is useful and
efficient when one of the parties of secret sharing is remote to the others who
are in adjacent, especially the parties are more than three. We describe the
process of this protocol and discuss its security when the quantum information
carrying is polarized single photons running circularly. It will be shown that
entanglement is not necessary for quantum secret sharing. Moreover, the
theoretic efficiency is improved to approach 100% as almost all the instances
can be used for generating the private key, and each photon can carry one bit
of information without quantum storage. It is straightforwardly to utilize this
topological structure to complete quantum secret sharing with multi-level
two-particle entanglement in high capacity securely.Comment: 7 pages, 2 figure
Probabilistic teleportation of unknown two-particle state via POVM
We propose a scheme for probabilistic teleportation of unknown two-particle
state with partly entangled four-particle state via POVM. In this scheme the
teleportation of unknown two-particle state can be realized with certain
probability by performing two Bell state measurements, a proper POVM and a
unitary transformation.Comment: 5 pages, no figur
Helicoverpa armigera nucleopolyhedrovirus occlusion-derived virus-associated protein, HA100, affects oral infectivity in vivo but not virus replication in vitro
ORF100 (ha100) of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) has been reported as one of the unique genes of group II alphabaculoviruses encoding a protein located in the occlusion-derived virus (ODV) envelope and nucleocapsid. The protein consists of 510 aa with a predicted mass of 58.1 kDa and is a homologue of poly(ADP–ribose) glycohydrolase in eukaryotes. Western blot analysis detected a 60 kDa band in HearNPV-infected HzAM1 cells starting at 18 h post-infection. Transient expression of GFP-fused HA100 in HzAM1 cells resulted in cytoplasmic localization of the protein, but after superinfection with HearNPV, GFPfused HA100 was localized in the nucleus. To study the function of HA100 further, an ha100-null virus was constructed using bacmid technology. Viral one-step growth curve analyses showed that the ha100-null virus had similar budded virus production kinetics to that of the parental virus. Electron microscopy revealed that deletion of HA100 did not alter the morphology of ODVs or occlusion bodies (OBs). However, bioassays in larvae showed that the 50% lethal concentration (LC50) value of HA100-null OBs was significantly higher than that of parental OBs; the median lethal time (LT50) of ha100-null OBs was about 24 h later than control virus. These results indicate that HA100 is not essential for virus replication in vitro. However, it significantly affects the oral infectivity of OBs in host insects, suggesting that the association HA100 with the ODV contributes to the infectivity of OBs in vivo
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