Protein destruction by a helium atmospheric pressure glow discharge: capability and mechanisms

Biological sterilization represents one of the most exciting applications of atmospheric pressure glow discharges (APGD). Despite the fact that surgical instruments are contaminated by both microorganisms and proteinaceous matters, sterilization effects of APGD have so far been studied almost exclusively for microbial inactivation. This work presents the results of a detailed investigation of the capability of a helium-oxygen APGD to inactivate proteins deposited on stainless-steel surfaces. Using a laser-induced fluorescence technique for surface protein measurement, a maximum protein reduction of 4.5 logs is achieved by varying the amount of the oxygen admixture into the background helium gas. This corresponds to a minimum surface protein of 0.36 femtomole/mm2. It is found that plasma reduction of surface-borne protein is through protein destruction and degradation, and that its typically biphasic reduction kinetics is influenced largely by the thickness profile of the surface protein. Also presented is a complementary study of possible APGD protein inactivation mechanisms. By interplaying the protein inactivation kinetics with optical emission spectroscopy, it is shown that the main protein-destructing agents are excited atomic oxygen (via the 777 and 844 nm emission channels) and excited nitride oxide (via the 226, 236, and 246 nm emission channels). It is also demonstrated that the most effective protein reduction is achieved possibly through a synergistic effect between atomic oxygen and nitride oxide. This study is a useful step toward a full confirmation of the efficacy of APGD as a sterilization technology for surgical instruments contaminated by prion proteins

Assessing the damming effects on runoff using a multiple linear regression model: A case study of the Manwan Dam on the Lancang River

AbstractThe Lancang River in Yunnan Province, with a length of 1170km and a 1780-m drop from northwest to southeast, is the most controversial river in southwest China because 14 cascade hydropower stations have been planned on the main waterway. The Manwan Dam, the first of the 14 dams, began operating in 1993, and the associated downstream runoff may have been affected by its construction. To assess this impact, we first investigated the relationships between monthly runoff observed from the Gajiu station and meteorological data obtained from four meteorological gauging stations with a time-lag of 0-3 months over the pre-dam period (1957-2000). Second, we established and validated a multiple linear regression equation employing monthly meteorological and hydrological data during the pre-dam period. Finally, we simulated the monthly runoff after dam construction (1993-2000) using the established equations and assessed the impact of dam construction on runoff by comparing the observed actual monthly runoff with the simulated monthly runoff. Our results suggested a very high hydro-meteorological correlation for the pre-dam period, which opened up the possibility of runoff forecasting. Further, the multiple linear regression equation displayed good simulation performance as coefficient of determination (R2) and the Nash-Suttcliffe coefficient (NS) reached 0.84 and 0.82 respectively. By comparing the observed and the predicted monthly runoff, we found that construction of the Manwan Dam caused a visible disturbance on monthly runoff that, with the disturbance value, displayed a multi-peak fluctuation of up-down variation in the annual hydrologic regime circl

Formation of a molecular Bose-Einstein condensate and an entangled atomic gas by Feshbach resonance

Processes of association in an atomic Bose-Einstein condensate, and dissociation of the resulting molecular condensate, due to Feshbach resonance in a time-dependent magnetic field, are analyzed incorporating non-mean-field quantum corrections and inelastic collisions. Calculations for the Na atomic condensate demonstrate that there exist optimal conditions under which about 80% of the atomic population can be converted to a relatively long-lived molecular condensate (with lifetimes of 10 ms and more). Entangled atoms in two-mode squeezed states (with noise reduction of about 30 dB) may also be formed by molecular dissociation. A gas of atoms in squeezed or entangled states can have applications in quantum computing, communications, and measurements.Comment: LaTeX, 5 pages with 4 figures, uses REVTeX

Thermal properties of coal during low temperature oxidation using a grey correlation method

The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.The low temperature oxidation of coal is a contradictory and unified dynamic process of coexisting mass and heat transfer. The thermophysical properties are crucial during coal spontaneous combustion. In the current paper, the variations of moisture, ash, volatiles, fixed carbon and thermophysical properties (thermal diffusivity, specific heat and thermal conductivity) of three coal samples from 30 °C to 300 °C were studied, and their grey correlation was analyzed. The results indicated that with the increase of temperature, the free moisture of Coals A and B decreased first but then increased, while the free moisture of Coal C kept decreasing without a later increase. The variation of surface moisture was consistent with that of free moisture. The trend of volatiles and fixed carbon was completely the opposite, showing a significant negative correlation. Ash was less affected by temperature. Along with the rise of temperature, the thermal diffusivity of three coal samples decreased first but later increased, and the specific heat was always in a state of increasing. The change in thermal conductivity was mainly affected by specific heat. By calculating the gray correlation degree, the major factors affecting the thermophysical properties were obtained

Two-Dimensional Spectroscopy of Photospheric Shear Flows in a Small delta Spot

In recent high-resolution observations of complex active regions, long-lasting and well-defined regions of strong flows were identified in major flares and associated with bright kernels of visible, near-infrared, and X-ray radiation. These flows, which occurred in the proximity of the magnetic neutral line, significantly contributed to the generation of magnetic shear. Signatures of these shear flows are strongly curved penumbral filaments, which are almost tangential to sunspot umbrae rather than exhibiting the typical radial filamentary structure. Solar active region NOAA 10756 was a moderately complex, beta-delta sunspot group, which provided an opportunity to extend previous studies of such shear flows to quieter settings. We conclude that shear flows are a common phenomenon in complex active regions and delta spots. However, they are not necessarily a prerequisite condition for flaring. Indeed, in the present observations, the photospheric shear flows along the magnetic neutral line are not related to any change of the local magnetic shear. We present high-resolution observations of NOAA 10756 obtained with the 65-cm vacuum reflector at Big Bear Solar Observatory (BBSO). Time series of speckle-reconstructed white-light images and two-dimensional spectroscopic data were combined to study the temporal evolution of the three-dimensional vector flow field in the beta-delta sunspot group. An hour-long data set of consistent high quality was obtained, which had a cadence of better than 30 seconds and sub-arcsecond spatial resolution.Comment: 23 pages, 6 gray-scale figures, 4 color figures, 2 tables, submitted to Solar Physic

Selection of antibody single-chain variable fragments with improved carbohydrate binding by phage display.

A single-chain variable fragment (Fv) version of a murine monoclonal antibody, Se155-4, specific for Salmonella serogroup B O-polysaccharide, was used as a model system for testing monovalent phage display as a route for enhancing the relatively low affinities that typify anti-carbohydrate antibodies. Random single-chain Fv mutant libraries generated by chemical and error-prone polymerase chain reaction methods were panned against the serogroup B lipopolysaccharide. Panning of a randomly mutated heavy chain variable domain library indicated selection for improved serogroup B binders and yielded six mutants, five of which showed wild type activity by enzyme immunoassay. Two of these were apparently selected on the basis of better functional single-chain Fv yield in Escherichia coli. A heavy chain mutation (Ile77-->Thr) in one mutant, 3B1, appeared to have a particularly dramatic effect, resulting in yields of approximately 120 mg/liter of functional periplasmic product. The sixth mutant, 4B2, had complementarity determining region 1 (CDR1) and CDR2 mutations and demonstrated 10-fold improved binding, by enzyme immunoassay, relative to the wild type. Extensive analysis of antigen-antibody interactions indicated that the improved binding properties of 4B2 were attributable to a higher association rate constant and interaction with an epitope that is larger than the trisaccharide recognized by the wild type. None of the mutations involved known trisaccharide contact residues; this was consistent with analysis of wild type and mutant single-chain Fvs by titration microcalorimetry. Examination of the structure indicated that two mutations in the heavy chain CDR2 provided improved surface complementarity between the protein and the extended epitope encompassing 2 additional hexose residues. However, introduction of only the CDR2 mutations into the wild type structure failed to confer the improved binding properties of 4B2, indicating an indirect effect by the more distant mutations. Panning of randomly mutated light chain variable domain and full-length single-chain Fv mutant libraries did not yield mutants with improved assembly or binding properties

Control of an atom laser using feedback

A generalised method of using feedback to control Bose-Einstein condensates is introduced. The condensates are modelled by the Gross-Pitaevskii equation, so only semiclassical fluctations can be suppressed, and back-action from the measurement is ignored. We show that for any available control, a feedback scheme can be found to reduce the energy while the appropriate moment is still dynamic. We demonstrate these schemes by considering a condensate trapped in a harmonic potential that can be modulated in strength and position. The formalism of our feedback scheme also allows the inclusion of certain types of non-linear controls. If the non-linear interaction between the atoms can be controlled via a Feshbach resonance, we show that the feedback process can operate with a much higher efficiency.Comment: 6 pages, 7 figure

A social cost-benefit analysis of two One Health interventions to prevent toxoplasmosis

In the Netherlands, toxoplasmosis ranks second in disease burden among foodborne pathogens with an estimated health loss of 1,900 Disability Adjusted Life Years and a cost-of-illness estimated at €45 million annually. Therefore, effective and preferably cost-effective preventive interventions are warranted. Freezing meat intended for raw or undercooked consumption and improving biosecurity in pig farms are promising interventions to prevent Toxoplasma gondii infections in humans. Putting these interventions into practice would expectedly reduce the number of infections; however, the net benefits for society are unknown. Stakeholders bearing the costs for these interventions will not necessary coincide with the ones having the benefits. We performed a Social Cost-Benefit Analysis to evaluate the net value of two potential interventions for the Dutch society. We assessed the costs and benefits of the two interventions and compared them with the current practice of education, especially during pregnancy. A ‘minimum scenario’ and a ‘maximum scenario’ was assumed, using input parameters with least benefits to society and input parameters with most benefits to society, respectively. For both interventions, we performed different scenario analyses. The freezing meat intervention was far more effective than the biosecurity intervention. Despite high freezing costs, freezing two meat products: steak tartare and mutton leg yielded net social benefits in both the minimum and maximum scenario, ranging from €10.6 million to €31 million for steak tartare and €0.6 million to €1.5 million for mutton leg. The biosecurity intervention would result in net costs in all scenarios ranging from €1 million to €2.5 million, due to high intervention costs and limited benefits. From a public health perspective (i.e. reducing the burden of toxoplasmosis) and the societal perspective (i.e. a net benefit for the Dutch society) freezing steak tartare and leg of mutton is to be considered

Polariton condensation and lasing in optical microcavities - the decoherence driven crossover

We explore the behaviour of a system which consists of a photon mode dipole coupled to a medium of two-level oscillators in a microcavity in the presence of decoherence. We consider two types of decoherence processes which are analogous to magnetic and non-magnetic impurities in superconductors. We study different phases of this system as the decoherence strength and the excitation density is changed. For a low decoherence we obtain a polariton condensate with comparable excitonic and photonic parts at low densities and a BCS-like state with bigger photon component due to the fermionic phase space filling effect at high densities. In both cases there is a large gap in the density of states. As the decoherence is increased the gap is broadened and suppressed, resulting in a gapless condensate and finally a suppression of the coherence in a low density regime and a laser at high density limit. A crossover between these regimes is studied in a self-consistent way analogous to the Abrikosov and Gor'kov theory of gapless superconductivity.Comment: 17 pages, 8 figures, submitted to PR

Photospheric Magnetic Field: Relationship Between North-South Asymmetry and Flux Imbalance

Photospheric magnetic fields were studied using the Kitt Peak synoptic maps for 1976-2003. Only strong magnetic fields (B>100 G) of the equatorial region were taken into account. The north-south asymmetry of the magnetic fluxes was considered as well as the imbalance between positive and negative fluxes. The north-south asymmetry displays a regular alternation of the dominant hemisphere during the solar cycle: the northern hemisphere dominated in the ascending phase, the southern one in the descending phase during Solar Cycles 21-23. The sign of the imbalance did not change during the 11 years from one polar-field reversal to the next and always coincided with the sign of the Sun's polar magnetic field in the northern hemisphere. The dominant sign of leading sunspots in one of the hemispheres determines the sign of the magnetic-flux imbalance. The sign of the north-south asymmetry of the magnetic fluxes and the sign of the imbalance of the positive and the negative fluxes are related to the quarter of the 22-year magnetic cycle where the magnetic configuration of the Sun remains constant (from the minimum where the sunspot sign changes according to Hale's law to the magnetic-field reversal and from the reversal to the minimum). The sign of the north-south asymmetry for the time interval considered was determined by the phase of the 11-year cycle (before or after the reversal); the sign of the imbalance of the positive and the negative fluxes depends on both the phase of the 11-year cycle and on the parity of the solar cycle. The results obtained demonstrate the connection of the magnetic fields in active regions with the Sun's polar magnetic field in the northern hemisphere.Comment: 24 pages, 12 figures, 2 table