52 research outputs found

    Multiplication et bulbaison in vitro du glaïeul (Gladiolus grandiflorus Hort.)

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    In vitro Multiplication and Bulb Formation of (Gladiolus grandiflorus Hort.). In order to set up a new bulb formation and micropropagation of gladiolus in vitro, trials were undertaken using two ornemental gladiolus cultivars 'Peter pears' and 'White friend ship'. The explants used are apical buds with 2 to 3 mm length taken from the cormes. In vitro initiation and multiplication were done for the two cultivars on Murashige and Skoog medium to which 2 mg. l-1 of BA and 0.5 mgl-1 of AIB were added. In vitro root development and bulb formation have been occurred in MS Agar Agar medium with 0.5 mg.l-1 of AIB 30 days later than the same MS medium containing 6% of saccharose and without Agar

    Effet de la salinité sur la fluorescence chlorophyllienne, la teneur en proline et la production florale de trois espèces ornementales

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    Effect of Chloride Sodium on Chlorophyll Fluorescence, Plant Proline Content and Flowers Production of Three Ornamental Species. Three ornamental species (Zinnia elegans, Tagetes patula and Petunia hybrida) were used to test sodium chloride effect on chlorophyll fluorescence, plant proline content and flowers production. Three treatments were used in this trial: 0, 2 and 4 g of Nacl.l-1 of irrigation water. The results showed that chlorophyll fluorescence was not affected by sodium chloride treatment but plant proline content increased and flowers production decreased as NaCl doses increase

    Caractéristiques de la croissance et de la production en fruits chez trois variétés de piment (Capsicum annuum L.) sous stress salin

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    Characteristics of the Growth and Fruits Production of Three Pepper Varieties Capsicum annuum L. under Saline Stress. This work aims to evaluate the salt tolerance of three pepper varieties differing by their precocity: pm797 early variety, beldi semi-early and baklouti late variety. The trials were carried out in pots under glazed greenhouse. The results showed that salinity reduced the growth and production. However, a varietal difference response to salt stress was observed between the studied varieties. Indeed, the varieties beldi and baklouti are characterized by their vigour in absence as in the presence of salt. On the other hand, early variety PM797 is characterized by the stability of production. This is highlighting the utility of early characters in salinity tolerance

    In silico analysis of alpha1-antitrypsin variants: The effects of a novel mutation

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    Alpha1-antitrypsin (AAT) is a highly polymorphic protein with more than 120 variants that are classified as normal (normal protein secretion), deficient (reduced circulating AAT level caused by defective secretion) or null (no protein secretion). Alpha1-antitrypsin deficiency, one of the most common genetic disorders, predisposes adults to pulmonary emphysema and, to a lesser extent, chronic liver disease and cirrhosis. In this report, we provide additional sequence data for alpha1-antitrypsin based on the characterization of a novel variant detected in a 53-year-old heterozygous patient with chronic obstructive pulmonary disease. The mutation occurred on a PI*M2 base allele and was characterized by a T → C transition at nt 97 in exon II that led to the replacement of phenylalanine by leucine (F33L). Since the mutation was found in the heterozygous state with the expression of a normally secreted variant (PI*M1) it was not possible to assess the pattern of F33L secretion. However, computational analyses based on evolutionary, structural and functional information indicated a reduction of 23 Å 3 in the side chain volume and the creation of a cavity in the protein hydrophobic core that likely disturbed the tridimensional structure and folding of AAT. The accuracy of the in silico prediction was confirmed by testing known mutations

    Impact of personality on educator attitudes towards open educational resources

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    The emergence of open educational resources (OER) represents one of the most significant educational developments in the 21st century. Given their capacity to be freely adapted, re-used, and shared in different contexts, OER expand the options for educators. This paper reports on an initial study concerning such choices in which educators’ personalities are investigated in relation to the OER adoption. Choosing open approaches has been shown to correlate with personal attitudes and several studies have highlighted the potential and need for investigating how personality might affect OER adoption. To address this gap, this study investigates the impact of educators’ personality differences in relation to OER adoption. The analysis focuses specifically on the perception towards OER and the intention to use OER using the Five-Factor Model (FFM) to identify educators’ personalities. Following a mixed methods approach, data collected from university educators using questionnaires (57 respondents) and interviews (15 respondents) are discussed in a two-stage hierarchical regression analysis. Demographic variables (age and gender) do not show any significant relationship. Findings reveal that while the explored five personality dimensions do not have an impact on the educator attitudes towards OER, they seem to have a significant impact on their intention to use OER. Specifically, only three personality dimensions – namely, extraversion, agreeableness, and openness – have a significant impact on the intention to use OER. This shows that ‘open attitude’ (mixing extraversion, and agreeableness, and openness) may be a fundamental prerequisite for educators to engage in open teaching practices, including the use of OER

    Alpha-1 antitrypsin gene polymorphism in Chronic Obstructive Pulmonary Disease (COPD)

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    Alpha-1-antitrypsin (AAT) plays an important role in the pathogenesis of emphysema, the pathological lesion underlying the majority of the manifestations of Chronic Obstructive Pulmonary Disease (COPD). In this study we tested the hypothesis that common AAT polymorphisms influence the risk of developing COPDs. We investigated PiM1 (Ala213Val), PiM2 (Arg101His), PiM3 (Glu376Asp), PiS (Glu264Val) and PiZ (Glu342Lys) SERPINA1 alleles in 100 COPD patients and 200 healthy controls. No significant differences were observed in allele frequencies between COPD patients and controls, neither did haplotype analysis show significant differences between the two groups. A cross-sectional study revealed no significant relationship between common SERPINA1 polymorphisms (PiM1, PiM2, PiM3) and the emphysematous type of COPD. In addition, FEV1 annual decline, determined during a two-year follow up period, revealed no difference among carriers of the tested polymorphisms

    Etude de la variabilité intraspécifique de tolérance au stress salin du pois chiche (Cicer arietinum L.) au stade germination

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    Study of the Intra-specific Variability Tolerance to Salt Stress of Chickpea (Cicer arietinum L.) in the Germination Stage. This study aims at comparing the germinative behaviour of some genotypes of chickpea (Cicer arietinum L.), in salt stress. For this, a test has been driven on eight genotypes whose seeds are submitted under different concentrations of NaCl (0; 34; 68 and 102 mM) in a temperature of 26 °C and a relative humidity of 93%. The measures concerned the kinetics, the speed and the final germination rate. The mobilization of the reserves and the reversibility of the effect of salt stress are also studied. The results suggest that the germinative capacity of the genotypes is not affected in 34 mM of NaCl. But in more elevated concentrations, it decreases and the speed of germination shows down. The increase of the salt concentration until 68 and 102 mM hinders the process of mobilization of the reserves and decreases the mean daily germination. The intraspecific variability of chickpea is relatively important. But, in presence as in absence of salt, the FLIP97-254C genotype showed the best germinative behaviour. During germination, salinity has two different effects on the chickpea seeds, the first are osmotic reversible, the second are toxic irreversible
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