Chartres, d'<i>Autrikon</i> à <i>Autricum</i>, cité des Carnutes : prémices et essor de l’urbanisation

International audienceExcavations carried out in Chartres during the last decade, systematically sequenced and dated thanks to ceramic studies, provide a collection of reference points for establishing the main stages that marked the town’s evolution during the 1st c. AD. Among these, two cemeteries predated the installation of the Roman city. Its road network and the buildings along it are a good marker for the phasing of its development. The public buildings were probably constructed from the middle of the 1st c. AD. The dating of the digging of the large ditch with embankments has for a long time been uncertain. It seems that it ought to be assigned to the 1st c. AD.Les fouilles réalisées à Chartres ces dix dernières années, systématiquement mises en séquences et datées par l’étude de la céramique, fournissent un corpus de sites de référence pour l’établissement des grandes phases marquant l’évolution de la ville durant le Ier s. apr. J.-C. Parmi ceux-ci, deux nécropoles précèdent l’installation de la ville romaine. Son réseau viaire et les bâtiments qui le bordent constituent un bon marqueur des rythmes de son développement. Les constructions publiques sont probablement édifiées à partir du milieu du Ier s. apr. J.-C. La datation du creusement du grand fossé à talus a longtemps posé question. Il semble devoir être rattaché au Ier s. apr. J.-C

New AMD3100 derivatives for CXCR4 chemokine receptor targeted molecular imaging studies: synthesis, anti-HIV-1 evaluation and binding affinities

CXCR4 is a target of growing interest for the development of new therapeutic drugs and imaging agents as its role in multiple disease states has been demonstrated. AMD3100, a CXCR4 chemokine receptor antagonist that is in current clinical use as a haematopoietic stem cell mobilising drug, has been widely studied for its anti-HIV properties, potential to inhibit metastatic spread of certain cancers and, more recently, its ability to chelate radiometals for nuclear imaging. In this study, AMD3100 is functionalised on the phenyl moiety to investigate the influence of the structural modification on the anti-HIV-1 properties and receptor affinity in competition with anti-CXCR4 monoclonal antibodies and the natural ligand for CXCR4, CXCL12. The effect of complexation of nickel(II) in the cyclam cavities has been investigated. Two amino derivatives were obtained and are suitable intermediates for conjugation reactions to obtain CXCR4 molecular imaging agents. A fluorescent probe (BODIPY) and a precursor for 18F (positron emitting isotope) radiolabelling were conjugated to validate this route to new CXCR4 imaging agents

Chartres, d’Autrikon à Autricum, cité des Carnutes : prémices et essor de l’urbanisation

Les fouilles réalisées à Chartres ces dix dernières années, systématiquement mises en séquences et datées par l’étude de la céramique, fournissent un corpus de sites de référence pour l’établissement des grandes phases marquant l’évolution de la ville durant le ier s. apr. J.-C. Parmi ceux-ci, deux nécropoles précèdent l’installation de la ville romaine. Son réseau viaire et les bâtiments qui le bordent constituent un bon marqueur des rythmes de son développement. Les constructions publiques sont probablement édifiées à partir du milieu du ier s. apr. J.-C. La datation du creusement du grand fossé à talus a longtemps posé question. Il semble devoir être rattaché au ier s. apr. J.-C.Excavations carried out in Chartres during the last decade, systematically sequenced and dated thanks to ceramic studies, provide a collection of reference points for establishing the main stages that marked the town’s evolution during the 1st c. AD. Among these, two cemeteries predated the installation of the Roman city. Its road network and the buildings along it are a good marker for the phasing of its development. The public buildings were probably constructed from the middle of the 1st c. AD. The dating of the digging of the large ditch with embankments has for a long time been uncertain. It seems that it ought to be assigned to the 1st c. AD

Chartres, d'<i>Autrikon</i> à <i>Autricum</i>, cité des Carnutes : prémices et essor de l’urbanisation

International audienceExcavations carried out in Chartres during the last decade, systematically sequenced and dated thanks to ceramic studies, provide a collection of reference points for establishing the main stages that marked the town’s evolution during the 1st c. AD. Among these, two cemeteries predated the installation of the Roman city. Its road network and the buildings along it are a good marker for the phasing of its development. The public buildings were probably constructed from the middle of the 1st c. AD. The dating of the digging of the large ditch with embankments has for a long time been uncertain. It seems that it ought to be assigned to the 1st c. AD.Les fouilles réalisées à Chartres ces dix dernières années, systématiquement mises en séquences et datées par l’étude de la céramique, fournissent un corpus de sites de référence pour l’établissement des grandes phases marquant l’évolution de la ville durant le Ier s. apr. J.-C. Parmi ceux-ci, deux nécropoles précèdent l’installation de la ville romaine. Son réseau viaire et les bâtiments qui le bordent constituent un bon marqueur des rythmes de son développement. Les constructions publiques sont probablement édifiées à partir du milieu du Ier s. apr. J.-C. La datation du creusement du grand fossé à talus a longtemps posé question. Il semble devoir être rattaché au Ier s. apr. J.-C

De la terre dans nos assiettes, ou des fours de Pistillus à la table de Lucullus

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Identifying three-way DNA junction-specific small-molecules

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AMD3100: A Versatile Platform for CXCR4 Targeting 68 Ga-Based Radiopharmaceuticals

International audienceCXCR4 is a G protein-coupled receptor (GPCR), which is overexpressed in numerous diseases, particularly in multiple cancers. Therefore, this receptor represents a valuable target for imaging and therapeutic purposes. Among the different approaches, which were developed for CXCR4 imaging, a CXCR4 antagonist biscyclam system (AMD3100, also called Mozobil), currently used in the clinic for the mobilization of hematopoietic stem cells, was radiolabeled with different radiometals such as 62Zn, 64Cu, 67Ga, or 99mTc. However, cyclam is not an ideal chelator for most of these radiometals, and could lead to the release of the radionuclide in vivo. In the current study, a new family of CXCR4 imaging agents is presented, in which AMD3100 is used as a carrier for specific delivery of an imaging reporter, i.e., a 68Ga complex for PET imaging. AMD3100 was functionalized on the phenyl moiety with different linkers, either ethylenediamine or diamino-polyethylene glycol 3 (PEG3). The resulting AMD3100 analogues were further coupled with two different chelators, 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) or 1,4,7-triazacyclononane-1-glutaric acid-4,7-acetic acid (NODAGA). Five potential CXCR4 targeting agents were obtained. The derived AMD3100-based ligands were labeled with 68Ga, highlighting the influence of the spacer nature on the 68Ga-labeling yield. The lipophilic character of the different systems was also investigated, as well as their affinity for the CXCR4 receptor. The most promising compound was further evaluated in vivo in H69 tumor xenografts by biodistribution and PET imaging studies, validating the proof of principle of our concept

Improvement of Adhesion Properties and Corrosion Resistance of Sol-Gel Coating on Zinc

Corrosion is a major problem for durability of many metals and alloys. Among the efficient classical surface treatments, chromate-based treatments must be banished from industrial use due to their toxicity. At the same time, sol-gel routes have demonstrated high potential to develop an efficient barrier effect against aggressive environments. By this process, the anti-corrosion property can be also associated to others in the case of the development of multi-functional hybrid coatings. In this paper, the main goal is precisely to improve both the corrosion resistance and the adhesion properties of phosphated zinc substrates by the deposition of a hybrid (organic-inorganic) sol-gel layer. To reach this double objective, a choice between two formulations 3-glycidoxypropyltrimethoxysilane (GPTMS)/aluminum-tri-sec-butoxide (ASB) and 3-(trimethoxysilyl)propylmethacrylate (MAP)/tetraethylorthosilicate (TEOS) was firstly made based on the results obtained by microstructural characterizations using SEM, optical analysis, and mechanical characterization such as shock and/or scratch tests (coupled to climatic chamber and salt spray exposure). Several investigations were performed in this study, and the best formulation and performances of the system were obtained by adding a new precursor (1-[3-(trimethoxysilyl)propyl]ureido-UPS) under controlled conditions, as detailed in this paper