Pengaruh Citra Perusahaan dan Kualitas Pelayanan terhadap Loyalitas Pelanggan pada Jasa Perhotelan ( Kasus Hotel Benteng Pekanbaru )

This study aims to determine the influence of corporate image and service quality oncustomer loyalty at the Hotel Fort Pekanbaru. The companys image as a variable (X1) quality ofservice as a variable (X2) and customer loyalty as (Y).The method in this research is descriptive and quantitative using SPSS 20, where sampleswere used that guests staying more than 3 times in the Hotel Fort Pekanbaru with respondents asmany as 99 people. To determine the number of samples used slovin formula, as well as asampling technique by purposive sampling. Data collection through questionnaires andinterviews.Results of analysis using multiple linear regression, t test and f test so that it can be seenthat the variable corporate image positive effect on customer loyalty, that the provision ofquality services significant effect on customer loyalty and significant influence of the companysimage and service quality on customer.Keywords: Corporate Image, Service Quality and Customer Loyaltyloyalty

Cryptocapsinepoxide-type Carotenoids from Red Mamey, Pouteria sapota

Three new carotenoids, cryptocapsin-5,6-epoxide, 3ʹ-deoxycapsanthin-5,6-epoxide, and cryptocapsin-5,8-epoxides, have been isolated from the ripe fruits of red mamey (Pouteria sapota). Cryptocapsin-5,6-epoxide was prepared by partial synthesis via epoxidation of cryptocapsin and the (5R,6S)- and (5S,6R)-stereoisomers were identified by HPLC-ECD analysis. Spectroscopic data of the natural (anti) and semisynthetic (syn) derivatives obtained by acid-catalyzed rearrangement of cryptocapsin-5,8-epoxide stereoisomers were compared for structural elucidation. Chiral HPLC separation of natural and semisynthetic samples of cryptocapsin-5,8-epoxides was performed and HPLC-ECD analysis allowed configurational assignment of the separated stereoisomers

Development of electrocardiogram intervals during growth of FVB/N neonate mice

<p>Abstract</p> <p>Background</p> <p>Electrocardiography remains the best diagnostic tool and therapeutic biomarker for a spectrum of pediatric diseases involving cardiac or autonomic nervous system defects. As genetic links to these disorders are established and transgenic mouse models produced in efforts to understand and treat them, there is a surprising lack of information on electrocardiograms (ECGs) and ECG abnormalities in neonate mice. This is likely due to the trauma and anaesthesia required of many legacy approaches to ECG recording in mice, exacerbated by the fragility of many mutant neonates. Here, we use a non-invasive system to characterize development of the heart rate and electrocardiogram throughout the growth of conscious neonate FVB/N mice.</p> <p>Results</p> <p>We examine ECG waveforms as early as two days after birth. At this point males and females demonstrate comparable heart rates that are 50% lower than adult mice. Neonatal mice exhibit very low heart rate variability. Within 12 days of birth PR, QRS and QTc interval durations are near adult values while heart rate continues to increase until weaning. Upon weaning FVB/N females quickly develop slower heart rates than males, though PR intervals are comparable between sexes until a later age. This suggests separate developmental events may contribute to these gender differences in electrocardiography.</p> <p>Conclusions</p> <p>We provide insight with a new level of detail to the natural course of heart rate establishment in neonate mice. ECG can now be conveniently and repeatedly used in neonatal mice. This should serve to be of broad utility, facilitating further investigations into development of a diverse group of diseases and therapeutics in preclinical mouse studies.</p

Blood-brain- barrier co-culture models to study nanoparticle penetration : focus on co-culture systems

The blood-brain barrier, as a physical, active transport and metabolic barrier represents the main obstacle in the treatment of central nervous system diseases. The field of nanoparticle delivery systems is rapidly developing and nanocarriers seem to be promising for drug delivery or targeting to the brain. For testing the toxicity, uptake and transcellular transport of nanoparticles culture models of the blood-brain barrier are widely used, including immortalized brain endothelial cell lines, primary brain endothelial cells in static or dynamic culture conditions, and in co-culture systems with glial cells and/or pericytes. This mini-review gives a brief summary of blood-brain barrier co-culture models that were used for testing nanocarriers, the types of different nanoparticle systems that were examined on blood-brain barrier models, and the advantages, limitations and suitability of the blood-brain barrier models for nanoparticle penetration studies

FOXA1 mutations alter pioneering activity, differentiation and prostate cancer phenotypes.

Mutations in the transcription factor FOXA1 define a unique subset of prostate cancers but the functional consequences of these mutations and whether they confer gain or loss of function is unknown1-9. Here, by annotating the landscape of FOXA1 mutations from 3,086 human prostate cancers, we define two hotspots in the forkhead domain: Wing2 (around 50% of all mutations) and the highly conserved DNA-contact residue R219 (around 5% of all mutations). Wing2 mutations are detected in adenocarcinomas at all stages, whereas R219 mutations are enriched in metastatic tumours with neuroendocrine histology. Interrogation of the biological properties of wild-type FOXA1 and fourteen FOXA1 mutants reveals gain of function in mouse prostate organoid proliferation assays. Twelve of these mutants, as well as wild-type FOXA1, promoted an exaggerated pro-luminal differentiation program, whereas two different R219 mutants blocked luminal differentiation and activated a mesenchymal and neuroendocrine transcriptional program. Assay for transposase-accessible chromatin using sequencing (ATAC-seq) of wild-type FOXA1 and representative Wing2 and R219 mutants revealed marked, mutant-specific changes in open chromatin at thousands of genomic loci and exposed sites of FOXA1 binding and associated increases in gene expression. Of note, ATAC-seq peaks in cells expressing R219 mutants lacked the canonical core FOXA1-binding motifs (GTAAAC/T) but were enriched for a related, non-canonical motif (GTAAAG/A), which was preferentially activated by R219-mutant FOXA1 in reporter assays. Thus, FOXA1 mutations alter its pioneering function and perturb normal luminal epithelial differentiation programs, providing further support for the role of lineage plasticity in cancer progression

FINANCIAL PERFORMANCE ANALYSIS WITH ECONOMIC VALUE ADDED METHOD OF PT. MUSTIKA RATU TBK

PT Mustika Ratu Tbk. is a modern cosmetics and herbal medicine company listed on the Indonesia Stock Exchange. The problems that exist in PT Mustika Ratu Tbk. is on the company's Economic Value Added seen from the value of Invested Capital, NOPAT, WACC, and Capital Charges companies that fluctuate during the period 2012-2016. The purpose of this research is to know the financial performance of PT Mustika Ratu Tbk. in terms of Economic Value Added period 2012-2016. The method of analysis used in this research is descriptive method. The data used in this research is secondary data. The object of this study is the financial statements of PT. Mustika Ratu Tbk consisting of Balance Sheet and Income Statement in 2012 until 2016. The data in this research is obtained through literature study and corporate documentation. The results showed that the Economic Value Added (EVA) in 2012 amounted to 1,142,908,461 can be assessed Positive because it is above the value 0 on a predetermined benchmark, which means there has been an economic value-added process for the company. In 2013 Economic Value Added (EVA) of-944,887,103 can be rated Negative because it is below the value 0 on the benchmark that has been set, meaning there is no process of economic value-added for the company. In 2014 Economic Value Added (EVA) of -11.210.007.738 can be rated Negative because it is below the value 0 on the benchmark that has been set, meaning there is no process of economic value-added for the company. In 2015 Economic Value Added (EVA) of -10.595.194.484 can be rated Negative because it is below the value 0 on the benchmarks that have been set, meaning there is no process of economic value-added for the company. In 2016 Economic Value Added (EVA) of -17.181.300.521 can be rated Negative because it is below the value 0 on the benchmark that has been set, meaning there is no process of economic value-added for the company. Keywords: Financial Performance, Economic Value Added.[Font bold dan hanya satu alinea

Impact of the SPOP Mutant Subtype on the Interpretation of Clinical Parameters in Prostate Cancer.

Purpose: Molecular characterization of prostate cancer, including The Cancer Genome Atlas, has revealed distinct subtypes with underlying genomic alterations. One of these core subtypes, SPOP (speckle-type POZ protein) mutant prostate cancer, has previously only been identifiable via DNA sequencing, which has made the impact on prognosis and routinely used risk stratification parameters unclear. Methods: We have developed a novel gene expression signature, classifier (Subclass Predictor Based on Transcriptional Data), and decision tree to predict the SPOP mutant subclass from RNA gene expression data and classify common prostate cancer molecular subtypes. We then validated and further interrogated the association of prostate cancer molecular subtypes with pathologic and clinical outcomes in retrospective and prospective cohorts of 8,158 patients. Results: The subclass predictor based on transcriptional data model showed high sensitivity and specificity in multiple cohorts across both RNA sequencing and microarray gene expression platforms. We predicted approximately 8% to 9% of cases to be SPOP mutant from both retrospective and prospective cohorts. We found that the SPOP mutant subclass was associated with lower frequency of positive margins, extraprostatic extension, and seminal vesicle invasion at prostatectomy; however, SPOP mutant cancers were associated with higher pretreatment serum prostate-specific antigen (PSA). The association between SPOP mutant status and higher PSA level was validated in three independent cohorts. Despite high pretreatment PSA, the SPOP mutant subtype was associated with a favorable prognosis with improved metastasis-free survival, particularly in patients with high-risk preoperative PSA levels. Conclusion: Using a novel gene expression model and a decision tree algorithm to define prostate cancer molecular subclasses, we found that the SPOP mutant subclass is associated with higher preoperative PSA, less adverse pathologic features, and favorable prognosis. These findings suggest a paradigm in which the interpretation of common risk stratification parameters, particularly PSA, may be influenced by the underlying molecular subtype of prostate cancer

The Proportion of the Ungrazed Area of the Pasture (PUP) Determines When Forage Intake and Diet Quality Decline in Grazing Systems

Grazing management has to deal with the spatial and temporal heterogeneity of pastures. In this context it is desirable to have a grazing management strategy that can be applied in a wide range of pasture conditions to control daily forage intake, diet quality and thus, animal performance. Sward height has been extensively studied and has been found not to be universally applicable to control the animal response as its relationship with intake changes with sward structure (Prache and Peyraud, 2001; Sollenberger and Burns, 2001) . Selective grazing is a universal phenomenon where, independently of pasture condition, cattle prefer the more nutritious and easily ingested top stratum of the pasture before consuming the deeper strata that impose a lower diet quality and greater restrictions on selective grazing. This study tested the hypothesis that forage intake and diet quality significantly decreases when the top selected stratum is removed across the entire area of the pasture (i.e. the proportion of pasture ungrazed)