STRIDE CHARACTERISTICS RELATED TO RUNNING VELOCITY IN MAXIMAL SPRINT RUNNING

In sprinting, athletes and coaches strive to increase running speed by means of general and specific training methods. As running velocity is always the product of stride length and stride frequency, in the end, all methods aim to improve one or both of these factors. The relation between stride length, stride rate and running velocity has been discussed in the literature from different points of view. Alexander and Goldspink (1977) analysed the movement speed and stride characteristics of mammals. As they wanted to compare mammals of different sizes, they transferred stride characteristics and speed into dimensionless parameters, taking into account gravity and the length of the leg. Their conclusions should also be valid for humans. But it is not clear if these formulas can predict stride characteristics in maximal sprint running. It was the purpose of this study to analyse whether stride rate and stride length in maximal sprint running are related to running velocity as proposed by Alexander and Goldspink. A better understanding of this relationship could probably help coaches in developing sprint training strategies. In this study twenty male physical education students performed a maximal sprint over 100 meter and seventeen female students ran a 40 meter sprint. Running speed was continuously recorded by means of a velocimeter. Surface electrodes were used to record the muscle activity of four thigh muscles. These EMGrecordings were used to determine the duration of each stride cycle and to calculate mean stride rate per 5 meter-interval. The length of the lower limbs (h) and the acceleration of free fall (g) are used in the definitions of dimensionless running velocity (dV), stride rate(dSR) and stride length (dSL). The average maximal velocity of the male sprinters was 9.37 ±0.52 m/s while the female sprinters attained 7.38± 0.52 m/s. The relation between stride length and running velocity in male 100 meter performance was determined by means of linear regression analysis: dV=0.178+(1.175*dSL). This means that 87% of variance in running velocity seems to be related to differences in stride length. On the other hand variance in stride rate explains less than 20% of the variance in running speed. These findings are confirmed by the results in the 40 meter sprint of the female group: dV=(1.172*dSL)-0.0830 with 80% of variance in dV being explained by variance in dSL and 20% of dV explained by dSR. In contrast to the findings reported in the literature we found a clear linear relationship between dSL and dV, and no significant correlation between dSR and dV. These findings were confirmed in two separate analyses: one with females and another with males. The discrepancies between the results in the literature and this study can probably be explained by the fact that we analyzed pure sprint performances, in contrast to most studies analyzing differences in running speeds ranging from jogging to sprinting. In all-out sprinting, stride rate in the second 5 meter interval is already close to the maximum stride rate; from this point on differences in running speed are mainly due to changes in stride length

STUDY OF PERFORMANCE RELATED STRENGTH TESTS FOR COMPETITION LEVEL SPRINTERS

Strength is a performance determining factor in sprinting. This study investigates the significance of a variety of isokinetic tests to control strength requirements for sprinters. Eighteen competition level sprinters &1i0 0 m-time = 10.94 s, = 0.22 s) ran a 40 meter sprint and performed 24 isokinetic strength tests on the PROMETT-system Static, concentric, eccentric and plyometric contractions were executed at velocities between 0 and 300 '1s for knee-extensors, knee flexors and ankle extensors. For each movement the torque at three different joint angles was recorded. As the performance determining factors change in relation to running distance, the correlation between the recorded torques and the running speed is graphically presented in relation to running distance (72 graphs). Per type of contraction the torque with the highest correlation with running speed was selected for further analysis. To interpret these graphs three phases are distinguished in a 40 meter sprint. Phase 1 is the phase of initial acceleration (from 0 to 10 m), phase 2 is the phase of continued acceleration (from 10 to 30 m) and phase 3 is the phase of maximum running speed (30 to 40 m). The common variance in torque and running speed data is quantified by means of the determinationcoefficient. The results indicate that isokinetic strength tests can be used to evaluate sprint related strength requirements at a competition level. 30 to 50 percent of variance in running speed within each of the three phases can be declared by a single isokinetic strength test. It may be concluded that the strength of the knee flexors determines 50 % of the variance within the phase of initial acceleration. Ankle extension torques explain 45 % of the variance in running speed within phase 2, and the strength of the knee extensors determines 33 % of variance in maximum running speed. It is also remarkable that for ankle extension only tests were selected with a high movement velocity (200°/s), while for knee extension tests were selected at lower velocities (65 and 130°/s)

Weight bearing exercise can elicit similar peak muscle activation as medium–high intensity resistance exercise in elderly women

Purpose: To assess whether stepping-based weight bearing exercise (WBE) can elicit peak activation of upper leg muscles similar to resistance exercise (RE) at an intensity required to induce strength gains in elderly women. Methods: Muscular activation of several upper leg muscles was measured during RE and WBE in a cohort of 19 healthy elderly women (69.3 ± 3.4 years). WBE consisted of forward and lateral stepping with step heights of 10, 20 and 30 cm. Muscular activation was compared to 60% of one-repetition maximum (1-RM) of congruent RE. Results: Peak activation during WBE was higher than RE at 60% 1-RM during forward and lateral stepping in vastus lateralis starting at 20 cm (p = 0.049 and p = 0.001), and biceps femoris at 30 cm step height (p = 0.024 and p = 0.030). Gluteus maximus peak activation matched RE at 60% 1-RM at 20 and 30 cm step height regardless of step direction (p ≥ 0.077). Peak activation of the rectus femoris and gluteus medius matched RE activation at 60% 1-RM during lateral stepping at 30 cm (p = 0.355 and p = 0.243, respectively) but not during forward stepping. WBE did not induce similar activation as RE in the semitendinosus. Conclusion: In WBE, most upper leg muscles were recruited at an equal or higher intensity than in RE at 60% 1-RM. Lateral stepping at 30 cm step height showed the highest training potential of all WBE’s applied

Bench stepping with incremental heights improves muscle volume, strength and functional performance in older women

Aim: Task-specific exercises such as bench stepping can improve functional ability and reduce falling incidents in older adults. However, such exercises are often not optimized to improve muscle volume and force-velocity characteristics. This study determined the effects of a 12-week stepping program using incremental step heights (STEEP), on muscle volume, strength, power, functional ability and balance performance in older women. Methods: Forty-five community-dwelling women (69y ± 4) were randomly assigned to the STEEP group or a non-training CONTROL group. Training intensity was primarily determined by step height, while training volume remained equal. Thigh muscle volume (CT-scan), force-velocity characteristics of the knee extensors (Biodex dynamometer) and functional ability (Short Physical Performance Battery, timed stair ascent, 10-m walk test and countermovement jump height) were determined pre- and post-intervention. In addition, 3D trunk accelerations were recorded at the lower back to assess balance during the Short Physical Performance Battery balance tests. Results: Two-way ANOVA showed that the STEEP program increased thigh muscle volume, knee extensor isometric peak torque, dynamic peak power, unloaded rate of velocity development and improved performance on all functional tests to a greater extent than CONTROL (p <.05), except the countermovement jump. No improvements were found for peak velocity and balance performance (p >.05). Conclusion: Our results indicate that bench step training with incremental step heights simultaneously improves functional ability, thigh muscle volume and force-velocity characteristics of the knee extensors in older women

Scoring schemes of palindrome clusters for more sensitive prediction of replication origins in herpesviruses

Many empirical studies show that there are unusual clusters of palindromes, closely spaced direct and inverted repeats around the replication origins of herpesviruses. In this paper, we introduce two new scoring schemes to quantify the spatial abundance of palindromes in a genomic sequence. Based on these scoring schemes, a computational method to predict the locations of replication origins is developed. When our predictions are compared with 39 known or annotated replication origins in 19 herpesviruses, close to 80% of the replication origins are located within 2% of the genome length. A list of predicted locations of replication origins in all the known herpesviruses with complete genome sequences is reported

Phase analysis in maximal sprinting: an investigation of step-to-step technical changes between the initial acceleration, transition and maximal velocity phases

The aim of this study was to investigate spatiotemporal and kinematic changes between the initial acceleration, transition and maximum velocity phases of a sprint. Sagittal plane kinematics from five experienced sprinters performing 50-m maximal sprints were collected using six HD-video cameras. Following manual digitising, spatiotemporal and kinematic variables at touchdown and toe-off were calculated. The start and end of the transition phase were identified using the step-to-step changes in centre of mass height and segment angles. Mean step-to-step changes of spatiotemporal and kinematic variables during each phase were calculated. Firstly, the study showed that if sufficient trials are available, step-to-step changes in shank and trunk angles might provide an appropriate measure to detect sprint phases in applied settings. However, given that changes in centre of mass height represent a more holistic measure, this was used to sub-divide the sprints into separate phases. Secondly, during the initial acceleration phase large step-to-step changes in touchdown kinematics were observed compared to the transition phase. At toe-off, step-to-step kinematic changes were consistent across the initial acceleration and transition phases before plateauing during the maximal velocity phase. These results provide coaches and practitioners with valuable insights into key differences between phases in maximal sprinting

Stage-Specific Inhibition of MHC Class I Presentation by the Epstein-Barr Virus BNLF2a Protein during Virus Lytic Cycle

gamma-herpesvirus Epstein-Barr virus (EBV) persists for life in infected individuals despite the presence of a strong immune response. During the lytic cycle of EBV many viral proteins are expressed, potentially allowing virally infected cells to be recognized and eliminated by CD8+ T cells. We have recently identified an immune evasion protein encoded by EBV, BNLF2a, which is expressed in early phase lytic replication and inhibits peptide- and ATP-binding functions of the transporter associated with antigen processing. Ectopic expression of BNLF2a causes decreased surface MHC class I expression and inhibits the presentation of indicator antigens to CD8+ T cells. Here we sought to examine the influence of BNLF2a when expressed naturally during EBV lytic replication. We generated a BNLF2a-deleted recombinant EBV (ΔBNLF2a) and compared the ability of ΔBNLF2a and wild-type EBV-transformed B cell lines to be recognized by CD8+ T cell clones specific for EBV-encoded immediate early, early and late lytic antigens. Epitopes derived from immediate early and early expressed proteins were better recognized when presented by ΔBNLF2a transformed cells compared to wild-type virus transformants. However, recognition of late antigens by CD8+ T cells remained equally poor when presented by both wild-type and ΔBNLF2a cell targets. Analysis of BNLF2a and target protein expression kinetics showed that although BNLF2a is expressed during early phase replication, it is expressed at a time when there is an upregulation of immediate early proteins and initiation of early protein synthesis. Interestingly, BNLF2a protein expression was found to be lost by late lytic cycle yet ΔBNLF2a-transformed cells in late stage replication downregulated surface MHC class I to a similar extent as wild-type EBV-transformed cells. These data show that BNLF2a-mediated expression is stage-specific, affecting presentation of immediate early and early proteins, and that other evasion mechanisms operate later in the lytic cycle

The Epstein-Barr Virus G-Protein-Coupled Receptor Contributes to Immune Evasion by Targeting MHC Class I Molecules for Degradation

Epstein-Barr virus (EBV) is a human herpesvirus that persists as a largely subclinical infection in the vast majority of adults worldwide. Recent evidence indicates that an important component of the persistence strategy involves active interference with the MHC class I antigen processing pathway during the lytic replication cycle. We have now identified a novel role for the lytic cycle gene, BILF1, which encodes a glycoprotein with the properties of a constitutive signaling G-protein-coupled receptor (GPCR). BILF1 reduced the levels of MHC class I at the cell surface and inhibited CD8+ T cell recognition of endogenous target antigens. The underlying mechanism involves physical association of BILF1 with MHC class I molecules, an increased turnover from the cell surface, and enhanced degradation via lysosomal proteases. The BILF1 protein of the closely related CeHV15 c1-herpesvirus of the Rhesus Old World primate (80% amino acid sequence identity) downregulated surface MHC class I similarly to EBV BILF1. Amongst the human herpesviruses, the GPCR encoded by the ORF74 of the KSHV c2-herpesvirus is most closely related to EBV BILF1 (15% amino acid sequence identity) but did not affect levels of surface MHC class I. An engineered mutant of BILF1 that was unable to activate G protein signaling pathways retained the ability to downregulate MHC class I, indicating that the immune-modulating and GPCR-signaling properties are two distinct functions of BILF1. These findings extend our understanding of the normal biology of an important human pathogen. The discovery of a third EBV lytic cycle gene that cooperates to interfere with MHC class I antigen processing underscores the importance of the need for EBV to be able to evade CD8+ T cell responses during the lytic replication cycle, at a time when such a large number of potential viral targets are expressed

Plasmablastic lymphoma mimicking orbital cellulitis

Introduction Orbital cellulitis is an uncommon, potentially devastating condition that, when not promptly and adequately treated, can lead to serious sequelae. The presenting clinical signs are proptosis, swelling, ophthalmoplegia, pain and redness of the peri-orbital tissues. A number of case