A 100 mW monolithic Yb waveguide laser fabricated using the femtosecond laser direct-write technique

A femtosecond laser-written monolithic waveguide laser (WGL) oscillator based on a distributed feedback (DFB) architecture and fabricated in ytterbium doped phosphate glass is reported. The device lased at 1033 nm with an output power of 102 mW and a bandwidth less than 2 pm when bidirectionally pumped at 976 nm. The WGL device was stable and operated for 50 hours without degradation. This demonstration of a high performance WGL opens the possibility for creating a variety of narrow-linewidth laser designs in bulk glasses.Comment: 5 pages, 3 figures, submitted journal manuscrip

e-Learning cookbook. TPACK in professional development in higher education

Information and communication technology (ICT) makes it possible to bring information to everyone who wants to learn. Rapid advances in technology offer strong support for using ICT in teaching. Online education can intensify and improve students' learning process, and enables us to reach more students than by traditional means. The number of courses and modules being offered online is increasing rapidly worldwide. Although online education can reach more people nowadays and new and challenging learning experiences can be created with it, in the average university course the digital dimension too often remains limited to simply publishing the existing face-to-face course content online. It is crucial that lecturers have and can obtain knowledge about how to design technology-enhanced teaching. Technical advances can be expected to continue in the future, and those who wish to implement educational technology in their own teaching practice must reckon on becoming lifelong learners. This fits the culture of academic teachers perfectly: they are already lifelong learners and creators of new knowledge within their discipline. This book is based on the notion that a lecturer who uses ICT in teaching must learn how to apply his or her knowledge about content, pedagogy and technology in an integrated manner. The idea of integrating these three types of knowledge is based on the TPACK model, which stands for Technological Pedagogical Content Knowledge model. The material for this book was developed in a Dutch higher education innovation project known as MARCHET (Make Relevant Choices in Educational Technology, MARCHET, 2009-2011)

e-Learning cookbook. TPACK in professional development in higher education

Information and communication technology (ICT) makes it possible to bring information to everyone who wants to learn. Rapid advances in technology offer strong support for using ICT in teaching. Online education can intensify and improve students' learning process, and enables us to reach more students than by traditional means. The number of courses and modules being offered online is increasing rapidly worldwide. Although online education can reach more people nowadays and new and challenging learning experiences can be created with it, in the average university course the digital dimension too often remains limited to simply publishing the existing face-to-face course content online. It is crucial that lecturers have and can obtain knowledge about how to design technology-enhanced teaching. Technical advances can be expected to continue in the future, and those who wish to implement educational technology in their own teaching practice must reckon on becoming lifelong learners. This fits the culture of academic teachers perfectly: they are already lifelong learners and creators of new knowledge within their discipline. This book is based on the notion that a lecturer who uses ICT in teaching must learn how to apply his or her knowledge about content, pedagogy and technology in an integrated manner. The idea of integrating these three types of knowledge is based on the TPACK model, which stands for Technological Pedagogical Content Knowledge model. The material for this book was developed in a Dutch higher education innovation project known as MARCHET (Make Relevant Choices in Educational Technology, MARCHET, 2009-2011)

Inflammation and premature aging in advanced chronic kidney disease

Systemic inflammation in end-stage renal disease (ESRD) is an established risk factor for mortality and a catalyst for other complications which are related to a premature aging phenotype, including muscle wasting, vascular calcification and other forms of premature vascular disease, depression, osteoporosis and frailty. Uremic inflammation is also mechanistically related to mechanisms involved in the aging process, such as telomere shortening, mitochondrial dysfunction, and altered nutrient sensing, which can have direct effect on cellular and tissue function. In addition to uremia-specific causes such as abnormalities in the phosphate- Klotho axis, there are remarkable similarities between the pathophysiology of uremic inflammation and so-called "inflammaging" in the general population. Potentially relevant, but still somewhat unexplored in this respect are abnormal or misplaced protein structures as well as abnormalities in tissue homeostasis, which evoke danger signals through damage associated molecular patters (DAMPS) as well as the senescence associated secretory phenotype (SASP). Systemic inflammation, in combination with the loss of kidney function, can impair the resilience of the body to external and internal stressors by reduced functional and structural tissue reserve, and by impairing normal organ crosstalk, thus providing an explanation for the greatly increased risk of homeostatic breakdown in this population. In this review, the relation between uremic inflammation and a premature aging phenotype, as well as potential causes and consequences are discussed

A Directly-Written Monolithic Waveguide-Laser Incorporating a DFB Waveguide-Bragg Grating

We report the fabrication and performance of the first C-band directly-written monolithic waveguide-laser. The waveguide-laser device was created in an Erbium and Ytterbium doped phosphate glass host and consisted of an optical waveguide that included a distributed feedback Bragg grating structure. The femtosecond laser direct-write technique was used to create both the waveguide and the waveguide-Bragg grating simultaneously and in a single processing step. The waveguide-laser was optically pumped at approximately 980 nm and lased at 1537nm with a bandwidth of less than 4 pm.Comment: 6 pages, 13 references, 4 figure

Classical, novel and atypical isoforms of PKC stimulate ANF- and TRE/AP-1-regulated-promoter activity in ventricular cardiomyocytes

Cultured neonatal rat ventricular myocytes were co-transfected with expression plasmids encoding protein kinase C (PKC) isoforms from each of the PKC subfamilies (classical PKC-α, novel PKC-ε or atypical PKC-ξ) together with an atrial natriuretic factor (ANF) reporter plasmid. Each PKC had been rendered constitutively active by a single Ala→Glu mutation or a small deletion in the inhibitory pseudosubstrate site. cPKC-α, nPKC-ε or aPKC-ξ expression plasmids each stimulated ANF-promoter activity and expression of a reporter gene under the control of a 12-O-tetradecanoylphorbol 13-acetate-response element (TRE). Upregulation of the ANF promoter is characteristic of the hypertrophic response in the heart ventricle and a TRE is present in the ANF promoter. Thus all subfamilies of PKC may have the potential to contribute to hypertrophic response in cardiomyocytes

Hybrid system modeling and identification of cell biology systems: perspectives and challenges

Abstract: Some perspectives and challenges in research on the crossing of system biology, hybrid system formulations and system identification are outlined. Emphasis is given on the hybrid, gray box modeling of interactions between the different abstract levels of organization typically recognized in (micro-)organisms, its associated identification problem and optimal experimental (input) design

Label-free peptide profiling of Orbitrap™ full mass spectra

Background. We developed a new version of the open source software package Peptrix that can yet compare large numbers of Orbitrap™ LC-MS data. The peptide profiling results for Peptrix on MS1 spectra were compared with those obtained from a small selection of open source and commercial software packages: msInspect, Sieve™ and Progenesis™. The properties compared in these packages were speed, total number of detected masses, redundancy of masses, reproducibility in numbers and CV of intensity, overlap of masses, and differences in peptide peak intensities. Reproducibility measurements were taken for the different MS1 software applications by measuring in triplicate a complex peptide mixture of immunoglobulin on the Orbitrap™ mass spectrometer. Values of peptide masses detected from the high intensity peaks of the MS1 spectra by peptide profiling were verified with values of the MS2 fragmented and sequenced masses that resulted in protein identifications with a significant score. Findings. Peptrix finds about the same number of peptide features as the other packages, but peptide masses are in some cases approximately 5 to 10 times less redundant present in the peptide profile matrix. The Peptrix profile matrix displays the largest overlap when comparing the number of masses in a pair between two software applications. The overlap of peptide masses between software packages of low intensity peaks in the spectra is remarkably low with about 50% of the detected masses in the individual packages. Peptrix does not differ from the other packages in detecting 96% of the masses that relate to highly abundant sequenced proteins. MS1 peak intensities vary between the applications in a non linear way as they are not processed using the same method. Conclusions. Peptrix is capable of peptide profiling using Orbitrap™ files and finding differential expressed peptides in body fluid and tissue samples. The number of peptide masses detected in Orbitrap™ files can be increased by using more MS1 peptide profiling applications, including Peptrix, since it appears from the comparison of Peptrix with the other applications that all software packages have likely a high false negative rate of low intensity peptide peaks (missing peptides)