586 research outputs found
Goodwill Columbus
PRIMARY CONTACT:Â Andrew Dearth ([email protected])Goodwill Columbus offers volunteer opportunities for individuals, businesses and corporate groups as rewarding and varied as its mission of transforming the lives of individuals with disabilities and other barriers through pathways to independence and the power of work. With a commitment of anything from a few hours per week to the whole day, you or a group of your co-workers may choose to participate in a wide variety of volunteer opportunities here at Goodwill Columbus
Development of a Sandwich Hybridization Assay for the Harmful Cyanobacteria Microcystis spp.
Cyanobacteria are responsible for the largest number of harmful blooms (HABs) worldwide. HABs caused by the genus Microcystis pose public health threats because they often occur within close proximity to humans and produce microcystin (hepatotoxin), which can contaminate drinking water and recreational areas. Novel molecular techniques facilitate monitoring, ‘early warnings’ of HAB events, and appropriate management responses. Sandwich hybridization assay (SHA), the technique considered here, directly (no amplification) identifies and quantifies planktonic species using ribosomal RNA (rRNA)-targeted oligonucleotides. This project developed SHA for Microcystis 16S rRNA and validated the assay using laboratory cultures and samples from a multi-specific cyanobacteria bloom in a coastal stormwater detention pond. The assay calibration curve and limits of detection were determined using M. aeruginosa culture, though interspecific (M. aeruginosa, M. botrys, M. wesenbergii) comparisons revealed significantly (p \u3c 0.05) different responses for a comparable cell density. Assessments of three light intensities (40, 60, 100 µmol photons m-2 s-1) and two temperatures (25, 32˚C) showed that M. aeruginosa SHA response at 25˚C decreased with light intensity, but varied at 32˚C, with a significant (p \u3c 0.05) interaction between light intensity and temperature. A multi-specific cyanobacteria bloom of the genera Anabaena, Anabaenopsis, and Microcystis occurred during summer 2016. Microcystis abundance was not significantly (p \u3e 0.05) correlated with any form of measured nutrients, but was positively and significantly (p \u3c 0.05) correlated with microcystin concentration. The newly developed SHA successfully detected Microcystis from environmental samples, showing its potential for its integration with cyanoHAB monitoring efforts
Letter from Allan Dearth to James B. Finley
Dearth writes asking Finley to inquire into the spiritual well-being of prisoner John Faris, who was converted in the Methodist church in Springboro. He fell in with bad company and ended up in prison. Dearth has met with him in prison and learned that he is penitent, and requests Finley to help him. Faris desperately needs the help of the prison chaplain. Dearth also reports that the church at Red Lion is growing. Abstract Number - 1091https://digitalcommons.owu.edu/finley-letters/2075/thumbnail.jp
Nitric oxide-assisted atmospheric pressure corona discharge ionization for the analysis of automobile hydrocarbon emission species
AbstractNitric oxide reagent gas has been found to improve the sensitivty and robustness of the atmospheric pressure corona discharge ionization (APCDI) process. Sensitivity has been increased by a factor of 20-100, depending on the compound, over APCDI without nitric oxide. The robustness (defined as the sensitivity to matrix interferences) of APCDI in the presence of water has been improved by a factor of 3 over normal APCDI. These improvements are due in part to a modification of the commercial inlet system and ionization chamber that allows the chamber and sample gases to be heated to 100 and 350 °C, respectively. Nitric oxide was chosen as the reagent gas because of the variety and selectivity of its interaction with hydrocarbons with differing functional groups. Product ions of nitric oxide ionization and their subsequent tandem mass spectra are presented and discussed for selected alkanes, alkenes, alkylbenzenes, alcohols, aldehydes, and an ether. A tandem mass spectrometry (unique parent ion-daughter ion transition) method was developed to quantify componds of specific interest in vehicle emissions. The absolute sensitivty for these compounds, under ideal conditions, was determined and ranges from 0.006 ppb for xylene (most sensitive) to 80 ppb for C8 (or larger) normal alkanes. Routine sensitivity for real-world samples was in the single parts per billion range for aromatic and olefinic species. Potential applications include the real-time, on-line monitoring of selected hydrocarbons in automobile exhaust
Mexx - An Attitude, A Lifestyle, A Kiss: A Case Study In Global Strategy
This case explores the opportunities and challenges confronting Mexx in the early 21st century. For more than 20 years, Mexx, an Amsterdam-based global retailer, grew quickly and successfully. Purchased by the Liz Claiborne organization in 2001, at the turn of the century, Mexx was poised for continued expansion and support to build a powerful, global retail brand. In 2008, Mexx management faces strategic decisions that will impact the company’s future in the highly competitive global fashion arena
Highly chlorinated dimethanofluorenes in technical chlordane and in human adipose tissue
AbstractSeveral new components of technical chlordane were discovered using electron capture, negative ionization gas chromatographic mass spectrometry. These compounds have 10–12 chlorines and are produced by the condensation of three cyclopentadiene molecules. The most abundant compound has a molecular weight of 606 and has the elemental composition C15H6Cl12. This compound and a series of related compounds were also identified as contaminants in human adipose tissue samples. These compounds are approximately 0.01–0.03% of the technical chlordane mixture, and they have average concentrations in human adipose tissue of 0.4–0.7 ng per gram of fat. They are more highly retained in human adipose tissue than chlordane-like compounds containing eight or fewer chlorine atoms
Population Dynamics of Off-Host Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) Larvae in Response to Habitat and Seasonality in South Texas
The cattle tick, Rhipicephalus microplus (Canestrini), is an economically destructive arthropod because of its ability to vector bovine babesiosis. It is known that cattle ticks can spend 80–90% of their lifecycle as questing larvae, yet the effect of climatic factors on their off-host behavior and survival is unclear. The goal of this study was to measure the effects of specific ecological factors on off-host questing larvae in nature. The study was conducted in a south Texas pasture over a two-year period, during which time larval populations were surveyed. Simultaneously, weather variables—precipitation, relative humidity, and ambient temperatures—were recorded. Larval survival rates varied among seasons, with the overall highest populations recorded in the spring and the lowest in the fall by a ratio of 20:1. In the winter, the larger numbers were collected from exposed habitats at a ratio of 6:1. Conversely, canopied habitats in the summer had 10-fold larger larval numbers. In the spring, exposed and canopied habitats showed no difference in tick larval survival rates. The results show that the interaction between season and habitat strongly influence off-host questing tick survival. Relative humidity was a key weather variable
Investigation of electrode materials for low frequency electric antennas in sea water
This paper describes and gives the results of an experiment to measure the D.C. and ULF (0.1 to 1 Hz) relative impedance in sea water of several materials considered as possible electrodes for a submerged electric dipole antenna. The results of the ac and dc impedance tests are given for Ag, Al, C, Cy, Ni, Pb, Pt, Sn, Ti, W, Zn and Stainless Steel, in the form of potential difference vs current and impedance vs frequency curves. Tests concerning the relative receiving impedance of Silver Silver-Chloride, Platinum Platinum-Black, carbon and zinc electrodes are reported. A comparison is made between the submerged magnetic loop and electric dipole antennas for characteristics of open circuit voltage, short circuit current, antenna gain, and noise. Based on the analytical and experimental results obtained recommendations are made to further test the impedance and noise characteristics of the Silver Silver-Chloride, Platinum Platinum- Black and carbon electrodes.http://archive.org/details/investigationofe00dearLieutenant Commander, United States NavyApproved for public release; distribution is unlimited
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