The role of tolerant genotypes and plant nutrients in the management of iron toxicity in lowland rice

Iron toxicity is a nutrient disorder associated with high concentrations of iron in soil solutions. Deficiencies of other nutrients, such as P, K, Ca, Mg and Zn, have been implicated in its occurrence in rice plants. Field experiments were carried out in 1992 and 1993 in Ivory Coast to evaluate the iron toxicity tolerance of promising rice cultivars available in West Africa, and to provide additional information for selecting breeding materials. Two sites, differing in their potential to cause iron toxicity, were used. Glasshouse and field studies were also conducted to test the role of other nutrients in the occurrence of iron toxicity. The results showed that genetic tolerance to iron toxicity can significantly improve rice production in iron-toxic soils, with some cultivars producing yields in excess of 5 t/ha. The application of N, P, K and Zn in the field decreased the uptake of iron in rice tops, and this can be a significant factor in the iron-toxicity tolerance of the cultivars

Groundwater Nitrogen Source Identification and Remediation in the Texas High Plains and Rolling Plains Regions

Nitrogen in groundwater, more specifically nitrate, is common in certain areas and is often associated with agricultural production or urban areas underlain by coarse soils. While the presence of nitrates in groundwater is not debated, the specific sources or cause of the elevated nitrate in these areas is often questioned. The Texas High Plains and Rolling Plains regions are two areas in the state where elevated nitrates are readily found in groundwater and such questions regarding its cause and source are raised. These areas include portions of the Ogallala and Seymour Aquifers which both exhibit elevated nitrates in certain areas. In an effort to address questions about sources and causes of elevated groundwater nitrate and to provide sound data on potential management strategies that can remediate groundwater nitrate levels, this project was developed. The primary objective was to identify sources of groundwater nitrate in the Texas High Plains and Rolling Plains and the secondary objective was to evaluate and demonstrate strategies and practices for reducing nitrate levels in these same areas. Collectively, this effort was able to provide insight into the potential sources of nitrate found in groundwater while also demonstrating how available nitrates can be captured as a beneficial resource and effectively removed from the underlying aquifer

Performance of the improved larger acceptance spectrometer: VAMOS++

International audienceMeasurements and ion optic calculations showed that the large momentum acceptance of the VAMOS spectrometer at GANIL could be further increased from $\sim$ 11% to $\sim$ 30% by suitably enlarging the dimensions of the detectors used at the focal plane. Such a new detection system built for the focal plane of VAMOS is described. It consists of larger area detectors (1000 mm × 150 mm) namely, a Multi-Wire Parallel Plate Avalanche Counter (MWPPAC), two drift chambers, a segmented ionization chamber and an array of Si detectors. Compared to the earlier existing system (VAMOS), we show that the new system (VAMOS++) has a dispersion-independent momentum acceptance . Additionally a start detector (MWPPAC) has been introduced near the target to further improve the mass resolution to $\sim$ 1/220. The performance of the VAMOS++ spectrometer is demonstrated using measurements of residues formed in the collisions of 129Xe at 967 MeV on 197Au

Space Environmental Testing of the Electrodynamic Dust Shield Technology

NASA's exploration missions to Mars and the moon may be jeopardized by dust that will adhere to surfaces of (a) Optical systems, viewports and solar panels, (b) Thermal radiators, (c) Instrumentation, and (d) Spacesuits. We have developed an active dust mitigation technology, the Electrodynamic Dust Shield, a multilayer coating that can remove dust and also prevents its accumulation Extensive testing in simulated laboratory environments and on a reduced gravity flight shows that high dust removal performance can be achieved Long duration exposure to the space environment as part of the MISSE-X payload will validate the technology for lunar missions

Purification and Activity Testing of the Full-Length YycFGHI Proteins of Staphylococcus aureus

Background: The YycFG two-component regulatory system (TCS) of Staphylococcus aureus represents the only essential TCS that is almost ubiquitously distributed in Gram-positive bacteria with a low G+C-content. YycG (WalK/VicK) is a sensor histidine-kinase and YycF (WalR/VicR) is the cognate response regulator. Both proteins play an important role in the biosynthesis of the cell envelope and mutations in these proteins have been involved in development of vancomycin and daptomycin resistance. Methodology/Principal Findings: Here we present high yield expression and purification of the full-length YycG and YycF proteins as well as of the auxiliary proteins YycH and YycI of Staphylococcus aureus. Activity tests of the YycG kinase and a mutated version, that harbours an Y306N exchange in its cytoplasmic PAS domain, in a detergent-micelle-model and a phosholipid-liposome-model showed kinase activity (autophosphorylation and phosphoryl group transfer to YycF) only in the presence of elevated concentrations of alkali salts. A direct comparison of the activity of the kinases in the liposomemodel indicated a higher activity of the mutated YycG kinase. Further experiments indicated that YycG responds to fluidity changes in its microenvironment. Conclusions/Significance: The combination of high yield expression, purification and activity testing of membrane and membrane-associated proteins provides an excellent experimental basis for further protein-protein interaction studies an

Characterisation of the Fibroblast Growth Factor Dependent Transcriptome in Early Development

BACKGROUND: FGF signaling has multiple roles in regulating processes in animal development, including the specification and patterning of the mesoderm. In addition, FGF signaling supports self renewal of human embryonic stem cells and is required for differentiation of murine embryonic stem cells into a number of lineages. METHODOLOGY/PRINCIPAL FINDINGS: Given the importance of FGF signaling in regulating development and stem cell behaviour, we aimed to identify the transcriptional targets of FGF signalling during early development in the vertebrate model Xenopus laevis. We analysed the effects on gene expression in embryos in which FGF signaling was inhibited by dominant negative FGF receptors. 67 genes positively regulated by FGF signaling and 16 genes negatively regulated by FGF signaling were identified. FGF target genes are expressed in distinct waves during the late blastula to early gastrula phase. Many of these genes are expressed in the early mesoderm and dorsal ectoderm. A widespread requirement for FGF in regulating genes expressed in the Spemann organizer is revealed. The FGF targets MKP1 and DUSP5 are shown to be negative regulators of FGF signaling in early Xenopus tissues. FoxD3 and Lin28, which are involved in regulating pluripotency in ES cells are shown to be down regulated when FGF signaling is blocked. CONCLUSIONS: We have undertaken a detailed analysis of FGF target genes which has generated a robust, well validated data set. We have found a widespread role for FGF signaling in regulating the expression of genes mediating the function of the Spemann organizer. In addition, we have found that the FGF targets MKP1 and DUSP5 are likely to contribute to the complex feedback loops involved in modulating responses to FGF signaling. We also find a link between FGF signaling and the expression of known regulators of pluripotency