El uso de los hornos pachamanca y guayra para la fundición en los Andes

El presente estudio ofrece una reseña sobre la tradición metalúrgica andina, discrimina al horno de pachamanca del horno guayra, así como de otros hornos, y trata sobre su uso para la fundición en los Andes. Se apoya en las descripciones aportadas por los cronistas españoles coloniales, arqueólogos, etc. Haciendo referencia a evidencias arqueológicas pertenecientes a los períodos prehispánicos y colonial que se presentan en los distritos mineros de Morococha (Huancavelica, Perú), Porco (Bolivia) y en algunos sitios del Noroeste de Argentina, etc. Cabe acotar que la temática abordada aporta al conocimiento de los hornos de fundición andinos y constituye una parte apenas divulgada del patrimonio minero

Molinos mineros de tradición andina

El presente trabajo contiene aportes para la clasificación tipológica de los molinos mineros utilizados en los Andes precolombinos. Se apoya en las descripciones que nos han legado los arqueólogos, ingenieros de minas, cronistas españoles, etc., con referencias a evidencias arqueológicas y etnográficas presentes en Cobres, Incahuasi, Rosario de Coyahuaima, Capillitas, Andalgalá y Tacuil (Argentina); Zapar (Chile); Porco y Potosí (Bolivia); así como en Santiago de Tulpo (Perú), etc. Es necesario destacar que la temática abordada contribuye al conocimiento de los molinos mineros y constituye un aspecto apenas divulgado del patrimonio minero andino. ABSTRACT This paper contains contributions on the typological classification of the Pre-Columbian Andean mining mills. It is based on the descriptions of archaeologists, mining engineers, Spanish chroniclers, etc., with references to archaeological and ethnographic evidences present in Cobres, Incahuasi, Rosario de Coyahuaima, Capillitas, Andalgalá and Tacuil (Argentina); Zapar (Chile); Porco and Potosi (Bolivia); as well as in Santiago de Tulpo (Peru), etc. It is necessary to emphasize that the topics treated contribute to the knowledge of the mining mills and constitutes a non well known aspect of the Andean mining heritage

YY1 overexpression is associated with poor prognosis and metastasis-free survival in patients suffering osteosarcoma

<p>Abstract</p> <p>Background</p> <p>The polycomb transcription factor Yin Yang 1 (YY1) overexpression can be causally implicated in experimental tumor growth and metastasization. To date, there is no clinical evidence of YY1 involvement in outcome of patients with osteosarcoma. Prognosis of osteosarcoma is still severe and only few patients survive beyond five years. We performed a prospective immunohistochemistry analysis to correlate YY1 immunostaining with metastatic development and survival in a selected homogeneous group of patients with osteosarcoma.</p> <p>Methods</p> <p>We studied 41 patients suffering from osteosarcoma (stage II-IVa). Multivariate analysis was performed using Cox proportional hazard regression to evaluate the correlation between YY1 expression and both metastasis development and mortality.</p> <p>Results</p> <p>YY1 protein is not usually present in normal bone; in contrast, a high number of patients (61%) showed a high score of YY1 positive cells (51-100%) and 39% had a low score (10-50% positive cells). No statistical difference was found in histology, anatomic sites, or response to chemotherapy between the two degrees of YY1 expression. Cox regression analysis demonstrated that the highest score of YY1 expression was predictive of both low metastasis-free survival (HR = 4.690, 95%CI = 1.079-20.396; p = 0.039) and poor overall survival (HR = 8.353, 95%CI = 1.863-37.451 p = 0.006) regardless of the effects of covariates such as age, gender, histology and chemonecrosis.</p> <p>Conclusion</p> <p>Overexpression of YY1 in primary site of osteosarcoma is associated with the occurrence of metastasis and poor clinical outcome.</p

Therapeutic targeting of the stem cell niche in experimental hindlimb ischemia.

21BACKGROUND: The custom microenvironment 'vascular niche' is a potential therapeutic target for several pathophysiological conditions. Osteoblasts regulate the hematopoietic stem cell niche, and activation of the parathyroid hormone (PTH) receptor can increase the number of cells mobilized into the bloodstream. METHODS: C57Bl/6 mice were randomly assigned treatment with granulocyte-colony stimulating factor (G-CSF), PTH, G-CSF plus PTH or saline. All mice underwent hindlimb ischemia. Blood flow was measured by laser Doppler imaging. Indices of capillary activity were determined by electron microscopy in muscle tissue. CD34(+) and Ki67(+) cells were detected and evaluated by immunofluorescence, apoptosis by TUNEL, surface antigen and endothelial progenitor cells by fluorescence-activated cell sorting analysis, and vascular endothelial growth factor-164 and angiopoietin-1 expression by reverse-transcriptase polymerase chain reaction. Frozen bone marrow sections were stained for antigen-specific B cells and fibronectin and analyzed by confocal laser scanning microscopy. RESULTS: Following mobilization induced by G-CSF treatment, mice also treated with PTH showed increases in blood flow, capillary density, nitrite/nitrate release, angiogenic factors and circulating progenitor cells, as well as reduced apoptosis, fibrosis, oxidative stress and inflammation in ischemic muscles. Furthermore, hematopoietic antigen-specific B cells in the bone marrow were also increased by G-CSF alone and in combination with PTH. CONCLUSIONS: PTH might increase the efficiency of hematopoietic stem-cell-based therapy in a recognized model of peripheral ischemia. Our translational experimental therapeutic targeting of the vascular niche points to novel clinical targets for the hematopoietic stem-cell treatment of ischemic vascular diseases.nonenoneNAPOLI C; WILLIAM-IGNARRO S; BYRNS R; BALESTRIERI ML; CRIMI E; FARZATI B; MANCINI FP; DE NIGRIS F; MATARAZZO A; D'AMORA M; ABBONDANZA C; FIORITO C; GIOVANE A; FLORIO A; VARRICCHIO E; PALAGIANO A; MINUCCI PB; TECCE MF; A. GIORDANO; PAVAN A; IGNARRO LJNapoli, C; WILLIAM IGNARRO, S; Byrns, R; Balestrieri, Ml; Crimi, E; Farzati, B; Mancini, Fp; DE NIGRIS, F; Matarazzo, A; D'Amora, M; Abbondanza, C; Fiorito, C; Giovane, A; Florio, A; Varricchio, E; Palagiano, A; Minucci, Pb; Tecce, Mf; Giordano, Antonio; Pavan, A; Ignarro, L