Approaches to supply chain logistics integration in the textile/clothing sector: an exploratory study in the Region of Campania

Given its potential to reduce lead times and total costs of operations, increase delivery speed, responsiveness and flexibility, and ultimately customer satisfaction, supply chain logistics integration can help to improve the competitiveness of the Italian textile and clothing (T/C) firms. However, few research works on supply chain management in general, and few studies on logistics integration in particular have focused their attention on this sector. This paper analyses the approaches to supply chain logistics integration adopted by T/C companies located in the Region of Campania (Southern Italy) through a qualitative approach based on case studies. The preliminary findings of the study highlight that the prevailing approach to supply chain logistics integration is limited to functional boundaries within the firm

Translation of dialect and cultural transfer : an analysis of Eduardo De Filippo’s theatre

The thesis sets out to examine cultural transfer from Neapolitan dialect into English, in the translations of plays by the contemporary Neapolitan playwright Eduardo De Filippo (1900-1984). It involves a comparative textual analysis of English translations of a selection of De Filippo’s plays in order to identify the translation strategies employed by each translator to represent Neapolitan cultural identity. Eduardo De Filippo can be defined as one of the most prominent contemporary Italian playwrights who employed dialect to portray characters who trespassed the boundaries of both Neapolitan and Italian society and to address social issues which were comprehensible to a vast public. In fact, his innovative contribution resided in the ability to bring vernacular theatre to national and international level. Thus the objective of the study is to bring to light the universality of De Filippo’s message albeit the limited linguistic medium and to show how his theatre is represented in the Anglo-Saxon milieu. The aim of previous critical studies on the matter has been to focus on the stage representations of De Filippo’s oeuvres, without particular emphasis on the analysis of the dialect. Drawing on a variety of theatre as well as translational frameworks (critical work on translation and in particular on theatre translation, the polysystem theory, the descriptive approach, anthropology, and sociolinguistics) I argue that dialect theatre represents an autonomous genre, separate from standard Italian theatre, which needs to be accounted for in translation, and in particular that the domestication of the language reduces the cultural impact of the original plays. The thesis is the first study to suggest that lexicological issues reflect the interpretation of the Neapolitan society in the translated texts and to provide evidence of the appropriation of Neapolitan culture by the receiving theatrical system through the linguistic choices made in translation

Júri simulado como estratégia lúdica para o desenvolvimento do protagonismo e da autonomia por alunos do ensino médio do Distrito Federal

Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Biológicas, Instituto de Física, Instituto de Química, Faculdade UnB Planaltina, 2019.Na Biologia, percebemos que determinados conteúdos programáticos exigem a exposição prática e lúdica para sua compreensão. O professor deve demonstrar criatividade ao planejar a metodologia que contemplará a participação ativa do educando, bem como a apreensão dessas abordagens, tendo a contextualização de situação-problema como norteadora desse processo. A presente pesquisa analisou as contribuições da aplicação do júri simulado como atividade lúdica para a promoção da autonomia e do protagonismo dos alunos do 3º ano do ensino médio para a aprendizagem de genética forense, na perspectiva da biotecnologia. Essa estratégia teve ainda a intenção de minimizar as dificuldades da abstração de conceitos da genética que devem ser trabalhados na referida série. A proposta foi aplicada em quatro turmas de uma escola pública situada no Recanto das Emas - DF. Os alunos foram divididos em equipes, nas quais cada um assumiu determinado papel no júri simulado, além de haver o notório apoio de um líder do grupo para facilitar a comunicação com os outros alunos e com a professora-pesquisadora. Algumas atividades antecederam as apresentações, quais foram: apresentação do projeto júri simulado; aulão interdisciplinar envolvendo as disciplinas de Biologia, História, Filosofia e Língua Portuguesa, que contribuíram com esclarecimentos a respeito dos regimes militares do Brasil e da Argentina, contextualizando, dessa forma, o caso fictício do júri simulado; sugestão de séries de TV para verificarem o trabalho dos cientistas e investigadores forenses; palestras temáticas com profissionais das áreas de Direito e Biologia. Percebemos a educação por projetos, trabalho interdisciplinar e maior envolvimento dos alunos como metodologias ativas eficientes para o aprendizado. Foram escolhidos, como instrumentos de análise e investigação quantitativa-qualitativa, o diário de bordo (individual e coletivo) elaborado pelos alunos e pela professora-pesquisadora, bem como os relatos do grupo de discussão. A pesquisa viabilizou a confirmação do desenvolvimento do protagonismo e autonomia, possibilitou a apreensão do conteúdo de genética forense na perspectiva da biotecnologia, além de ratificar a contribuição lúdica do júri simulado para o ensino.In Biology, it is possible to notice that some subjects demand, to their considerable understanding, the practical and playful exposition. To do such thing in a school context, the teacher needs to demonstrate creativity to plan the correct methodology that may stimulate students’ active participation on the matter, as well as it may imply the correct apprehension towards these types of approach, by considering the concrete cases, which might be capable of guide the learning process of the scholars. The aim of the present study is indicate the contributions of the enforcement of a simulated jury as a playful activity used in a school environment, in order to encourage high school students develop their independency in the academic life routine, such as their proactive attitudes in the genetic and forensic studies, considering a biotechnology perspective. Furthermore, it is crucial to consider, in the actual method, the intention of minimizing the difficulties that may appear on the process of abstracting the application of genetics knowledge by senior year students. That being said, the purpose of this paper work is applying the study in analysis on four classes of a public school located in Recanto das Emas – DF. Therefore, the pupils were divided in groups, in which each one of the them were prepared to take a party in the simulated jury system and in addition there was designated one leading role to establish an easier communication amongst the students and the research professor. For that matter, it was adopted the quantitative and qualitative methodology in order to achieve the main goals of the activities enrolled by this master’s thesis, identified as: presentation of the simulated jury’s project to the school community in which it would be applied; interdisciplinary approaches according to Biology, History, Philosophy and Portuguese subjects, that were deeply necessary in the contextualization towards the military dictatorship that took place in Brazil and in Argentina, considered the basic concept used to build concrete cases for the simulated jury; TV shows suggestions (especially CSI, Bones and Dexter) to get the idea of the work performed by forensic scientists and criminal investigators; and finally, talks promoted by Law and Biology professionals. As a result, it was genuinely verified that the methods used in the research were remarkably efficient to students’ learning process. In order to analyze and investigate the progress of the project, the students and the research professor needed to write a “log” or a diary containing not only the facts happened during the activities practiced but the discussions promoted by the group about the whole proposal. Thus, the research made possible the confirmation of the aforementioned thesis about the development of students’ independency in their academic life routine, such as their proactiveness in school assignments, also it made possible the considerable subject apprehension about genetics and forensic studies pursued by the biotechnology perspective, and likewise it made possible ascertain the playful contribution of the simulated jury in scholars’ education

A Stress Surveillance System Based on Calcium and Nitric Oxide in Marine Diatoms

Diatoms are an important group of eukaryotic phytoplankton, responsible for about 20% of global primary productivity. Study of the functional role of chemical signaling within phytoplankton assemblages is still in its infancy although recent reports in diatoms suggest the existence of chemical-based defense strategies. Here, we demonstrate how the accurate perception of diatom-derived reactive aldehydes can determine cell fate in diatoms. In particular, the aldehyde (2E,4E/Z)-decadienal (DD) can trigger intracellular calcium transients and the generation of nitric oxide (NO) by a calcium-dependent NO synthase-like activity, which results in cell death. However, pretreatment of cells with sublethal doses of aldehyde can induce resistance to subsequent lethal doses, which is reflected in an altered calcium signature and kinetics of NO production. We also present evidence for a DD–derived NO-based intercellular signaling system for the perception of stressed bystander cells. Based on these findings, we propose the existence of a sophisticated stress surveillance system in diatoms, which has important implications for understanding the cellular mechanisms responsible for acclimation versus death during phytoplankton bloom successions

Tinnitus sensation pre and post nutritional intervention in metabolic disorders

TEMA: diferentes etiologias estão relacionadas com a presença de zumbido, incluindo doenças metabólicas (glicêmicas e lipídicas). OBJETIVO: comparar o grau de severidade do zumbido por meio de medidas de auto-análise em sujeitos com alterações metabólicas pré e pós-intervenção nutricional, utilizando o Questionário de Gravidade do Zumbido. MÉTODO: vinte e um sujeitos, homens e mulheres, com idade entre 40 e 82 anos, participaram deste estudo. Critérios de inclusão abrangeram a presença de zumbido e de alteração metabólica diagnosticada por meio de exames laboratoriais. Todos os sujeitos foram submetidos a um programa de intervenção nutricional. A avaliação audiológia e o questionário de gravidade de zumbido foram aplicados pré e pós-intervenção. RESULTADOS: comparando os resultados pré e pós-intervenção os dados mostram uma diferença estatisticamente significante com relação à sensação do zumbido em 71,5% dos sujeitos, os quais referiram menor impacto do zumbido nas atividades diárias. CONCLUSÃO: uma importante diferença foi observada com relação à influência do zumbido na vida do sujeito quando utilizadas as medidas de auto-análise. Verificou-se uma relação direta entre zumbido e alterações metabólicas em casos relacionados a estes sintomas.BACKGROUND: different etiologies are related to tinnitus including metabolic disorders (blood glucose and lipids). AIM: the aim of this study was compare tinnitus severity by self-report measures pre and post nutritional intervention, using the Tinnitus Handicap Inventory. METHOD: participants of this study were twenty one male and female subjects, with ages ranging from 40 to 82 years. Inclusion criteria involved the presence of tinnitus and metabolic disorder diagnosed by laboratory exams. All subjects were submitted to a nutritional intervention program. Audiological evaluation and the Tinnitus Handicap Inventory were applied pre and post intervention. RESULTS: when comparing the presence of tinnitus pre and post intervention, data analysis indicates statistical difference concerning tinnitus sensation - 71.5% of the individuals referred less impact of tinnitus in daily activities. CONCLUSION: an important difference was observed concerning tinnitus influence in subject's life by self-report measures. A direct relation between tinnitus and metabolic disorders in cases related with this symptom was verified

A prospective study comparing quantitative Cytomegalovirus (CMV) polymerase chain reaction in plasma and pp65 antigenemia assay in monitoring patients after allogeneic stem cell transplantation

BACKGROUND: Low levels of Cytomegalovirus (CMV) viral load are frequently detected following allogeneic stem cell transplantation (SCT) and CMV disease may still develop in some allogeneic SCT patients who have negative pp65-antigenemia (pp65-Ag) or undetectable DNA. Pp65Ag is a sensitive method to diagnose CMV infection. Quantitative CMV-DNA PCR assay in plasma has been proposed to monitor CMV infection in SCT patients. We evaluated the clinical utility of pp65Ag and PCR assay in plasma of SCT recipients. METHODS: In a prospective longitudinal study, 38 consecutive patients at risk of CMV infection (donor and/or recipient CMV seropositive) were weekly monitored for CMV infection by both quantitative CMV-PCR in plasma (COBAS AMPLICOR CMV MONITOR) and pp65 Ag, during the first 100 days after SCT. RESULTS: A total of 534 blood samples were simultaneously analysed for pp65Ag and PCR. Overall, 28/38 patients (74%) had active CMV infection within 100 days from SCT. In 16 patients, CMV was first detected by pp65 Ag alone; in 5 patients by both methods and in 6 by PCR assay alone; one patient had CMV biopsy-proven intestinal disease without pp65Ag and PCR assays positivity before CMV disease. Overall, three patients developed intestinal CMV disease (7.9%): one had negative both pp65Ag and PCR assays before CMV disease, one had disease and concomitant positivity of both methods, while in the remaining patient, only pp65Ag was positive before CMV disease. CONCLUSION: Plasma PCR(COBAS AMPLICOR CMV MONITOR) and pp65Ag assays were effective in detecting CMV infection, however, discordance between both methods were frequently observed. Plasma PCR and pp65Ag assays may be complementary for diagnosis and management of CMV infection

Central-line associated bloodstream infections in a tertiary care children's University hospital: a prospective study

BACKGROUND: The central-line associated bloodstream infections (CLABSI) are the most common healthcare-associated infections in childhood. Despite the international data available on healthcare-associated infections in selected groups of patients, there is a lack of large and good quality studies. The present survey is the first prospective study monitoring for 6 months the occurrence of central-line associated bloodstream infections in all departments of an Italian tertiary care children’s university hospital. METHODS: The study involved all children aged less than 18 years admitted to Meyer Children’s University Hospital, Florence, Italy who had a central line access between the October 15(th), 2014 and the April 14(th), 2015. CLABSI were defined according to the Center for Disease Control and Prevention criteria. CLABSI incidence rates with 95% confidence limits were calculated and stratified for the study variables. For each factor the relative risk and 95% confidence intervals were evaluated. Statistical analysis was performed using the statistical software SPSS for Windows, version 22.0 (SPSS Inc., Chicago, IL), p < 0.05 was considered statistically significant. RESULTS: CLABSI rate was 3.73/1000 (95% CI: 2.54–5.28) central line-days. A higher CLABSI incidence was seen with female gender (p = 0.045) and underlying medical conditions (excepting prematurity, surgical diseases and malignancy) (p = 0.06). In our study 5 infections, were caused by extended-spectrum β-lactamase producing organisms and in one case by carbapenem-resistant Klebsiella pneumoniae. CONCLUSIONS: Our study confirms the spreading of multi-resistant pathogens as causes of healthcare associated infections in children. An increased incidence rate of CLABSI in our study was related to underlying medical conditions. Pediatric studies focusing on healthcare infections in this type of patients should be done in order to deepen our understanding on associated risk factors and possible intervention areas

Transcriptional Repressors of Fetal Globin Genes as Novel Therapeutic Targets in Beta-Thalassemia

During development the human β-globin gene cluster undergoes two switching processes at the embryo-fetal and fetal-adult stages, respectively, involving changes in chromatin remodeling and in transcriptional regulatory networks. In particular, during the perinatal period, the switch from fetal-to-adult globin gene expression leads to fetal globin genes silencing and progressive decline of fetal hemoglobin (HbF). Impaired hemoglobin switching is associated with hereditary persistence of HbF (HPFH), a condition in which the fetal globin genes fail to be completely silenced in adult red blood cells. This condition, when co-inherited with hemoglobinopathies, has great therapeutic potential because elevated HbF levels can ameliorate β-thalassemia and sickle cell anemia. Therefore, there is a growing interest about the complex network of factors that regulate fetal globin genes expression. Here we discuss the activity of transcriptional repressors of fetal globin genes and their potential role as novel therapeutic targets in β-thalassemia

Mycobacterial and Human Ferrous Nitrobindins: Spectroscopic and Reactivity Properties

Structural and functional properties of ferrous Mycobacterium tuberculosis (Mt-Nb) and human (Hs-Nb) nitrobindins (Nbs) were investigated. At pH 7.0 and 25.0 °C, the unliganded Fe(II) species is penta-coordinated and unlike most other hemoproteins no pH-dependence of its coordination was detected over the pH range between 2.2 and 7.0. Further, despite a very open distal side of the heme pocket (as also indicated by the vanishingly small geminate recombination of CO for both Nbs), which exposes the heme pocket to the bulk solvent, their reactivity toward ligands, such as CO and NO, is significantly slower than in most hemoproteins, envisaging either a proximal barrier for ligand binding and/or crowding of H2O molecules in the distal side of the heme pocket which impairs ligand binding to the heme Fe-atom. On the other hand, liganded species display already at pH 7.0 and 25 °C a severe weakening (in the case of CO) and a cleavage (in the case of NO) of the proximal Fe-His bond, suggesting that the ligand-linked movement of the Fe(II) atom onto the heme plane brings about a marked lengthening of the proximal Fe-imidazole bond, eventually leading to its rupture. This structural evidence is accompanied by a marked enhancement of both ligands dissociation rate constants. As a whole, these data clearly indicate that structural-functional relationships in Nbs strongly differ from what observed in mammalian and truncated hemoproteins, suggesting that Nbs play a functional role clearly distinct from other eukaryotic and prokaryotic hemoproteins

Remoção químico-mecânica da cárie dental com o uso de CarisolvTM

Introduction: dentistry is improving techniques for caries removal, intending to facilitate the clinical routine. Such advancement was the introduction of chemical-mechanical caries removal mechanisms. Traditionally, the carious lesion removal has been held by mechanical methods such as the use of rotary and manual cutting tools, which have been shown effective nevertheless, have disadvantages as pain and eventual removal of healthy tissue. Aim: study the advantages and disadvantages of a chemical-mechanical surgical technique when using the product CarisolvTM and know its composition and its acting mechanism. Conclusion: caries removal with CarisolvTM promotes both S.mutans as Lactobacillus elimination. In the meantime is a controversial process with regard to its effectiveness and pain absence because, eventually, it is necessary to use rotary cutting tools to complement the caries removal, has a high cost and consumes a larger clinical time when compared to dental caries mechanical removal.Introdução: a Odontologia vem aprimorando as técnicas de remoção do tecido cariado, visando facilitar a rotina clínica. Um desses avanços foi a introdução de mecanismos para a remoção químico-mecânica da cárie dental. Tradicionalmente, a remoção do tecido cariado tem sido realizada por meio de métodos mecânicos, como a utilização de instrumentos cortantes rotatórios e manuais que embora tenham se mostrado efetivos, apresentam desvantagens como a dor e eventual remoção de tecido sadio. Objetivo: estudar as vantagens e as desvantagens da técnica operatória de um método químico-mecânico quando se utiliza o produto CarisolvTM, bem como conhecer a sua composição e o seu mecanismo de ação.Conclusão: a remoção de tecido cariado com o CarisolvTM promove tanto a eliminação de S.mutans quanto a de Lactobacillus, entretanto é um processo controverso no que tange a sua eficácia e a ausência de dor visto que, eventualmente, é necessário o uso de instrumentos cortantes rotatórios para complementar a remoção de tecido cariado, possui um custo elevado e consome um maior tempo clínico quando comparado à remoção mecânica da cárie dental