Data Report: Pump Sampling and Sediment Analysis in Support of the Sensor Insertion System Duck, N.C. April and October, 1997

The objectives of this study were to (i) construct a pumping system, (ii) operate the system and collect samples of suspended sediment across the width of the surf zone during two major field experiments planned for the SIS in 1997, and (iii) analyze the resulting samples for sand concentration, sand size distribution, total percent sand, total percent mud, and organic content. The purpose was to provide a high quality data set of pump samples with which to later test the sensitivity of indirect measurements of suspended sand concentration to the presence of suspended mud. The response of OBSs is known to be particularly sensitive to the presence of suspended mud ( turbidity ) due to the inverse response of OBS output to grain size. Without better constraints on the proper interpretation of OBS time series, the overall conclusions with regards to net along-shelf transport of sand during storms may be in doubt (see following section)

Gevoeligheid van belgische Mycoplasma Hyopneumoniae isolaten voor antimicrobiële middelen

To control Mycoplasma hyopneumoniae infections, the use of antibiotics in feed or water is a common practice. Unfortunately, information concerning the susceptibility of M. hyopneumoniae to different antimicrobials is very scarce. The in vitro susceptibility of 21 M hyopneumoniae field isolates was determined in this study using a broth microdilution technique. Acquired resistance to spectinomycin, oxytetracycline, doxycycline, gentamicin, florfenicol and tiamulin was not observed. One isolate showed acquired resistance to lincomycin, tilmicosin and tylosin, but was susceptible to all other antibiotics tested. For 5 isolates, the MIC-values of flumequine were > 16 mu g/ml, while the MIC50-value was 2 mu g/ml. The MIC-values of enrofloxacin for these 5 isolates were >= 0.5 mu g/ml, the MIC50 being 0.06 mu g/ml. This is the first report showing acquired resistance against macrolides, lincosamides and fluoroquinolones in M hyopneumoniae field isolates

Subtractive isolation of phage-displayed single-chain antibodies to thymic stromal cells by using intact thymic fragments

In the murine thymus, the stroma forms microenvironments that control different steps in T cell development. To study the architecture of such microenvironments and more particularly the nature of communicative signals in lympho–stromal interaction during T cell development, we have employed the phage antibody display technology, with the specific aim of isolating thymic stromal cellspecific single-chain antibodies from a semisynthetic phage library. A subtractive approach using intact, mildly fixed thymic fragments as target tissue and lymphocytes as absorber cells generated monoclonal phages (MoPhabs) detecting subsets of murine thymic stromal cells. In the present paper we report on the reactivity of single-chain antibodies derived from three MoPhabs, TB4–4, TB4–20, and TB4–28. While TB4–4 and TB4–20 are both epithelium specific, TB4–28 detects an epitope expressed on both epithelial- and mesenchymal-derived stromal cells. TB4–4 reacts with all cortical epithelial cells and with other endoderm-derived epithelia, but this reagent leaves the majority of medullary epithelial cells unstained. In contrast, MoPhab TB4–20 detects both cortical and medullary thymic epithelial cells, as well as other endoderm- and ectoderm-derived epithelial cells. Cross-reaction of single-chain antibodies to human thymic stromal cells shows that our semisynthetic phage antibody display library, in combination with the present subtractive approach, permits detection of evolutionary conserved epitopes expressed on subsets of thymic stromal cells

Improving the evaluation of worldwide biomedical research output: classification method and standardised bibliometric indicators by disease

Merit, Expertise and Measuremen

Thixotropy in macroscopic suspensions of spheres

An experimental study of the viscosity of a macroscopic suspension, i.e. a suspension for which Brownian motion can be neglected, under steady shear is presented. The suspension is prepared with a high packing fraction and is density-matched in a Newtonian carrier fluid. The viscosity of the suspension depends on the shear rate and the time of shearing. It is shown for the first time that a macroscopic suspension shows thixotropic viscosity, i.e. shear-thinning with a long relaxation time as a unique function of shear. The relaxation times show a systematic decrease with increasing shear rate. These relaxation times are larger when decreasing the shear rates, compared to those observed after increasing the shear. The time scales involved are about 10000 times larger than the viscous time scale and about 1000 times smaller than the thermodynamic time scale. The structure of the suspension at the outer cylinder of a viscometer is monitored with a camera, showing the formation of a hexagonal structure. The temporal decrease of the viscosity under shear coincides with the formation of this hexagonal pattern

Implementatie van EU-handhavingsvoorschriften.

The politics and administration of institutional chang

Implementation of EU enforcement provisions: between European control and national practice. A report on Dutch experience

FDR Trias Europea: de verhoudingen tussen de overheidsmachten in de EU en de lidstaten in een bewegend constitutioneel landschap -- ou