Assessing the effectiveness of voluntary solid waste reduction policies: methodology and a Flemish case study

The purpose of this paper is to illustrate the use of statistical techniques to evaluate the effectiveness of voluntary policy instruments for waste management. The voluntary character of these instruments implies that latent characteristics, unobserved by the analyst, might influence the participation decision and might lead to biased estimates of the effectiveness of the policy instrument if standard techniques are used. We propose an extension of the Difference-in-Differences estimator to evaluate the effectiveness of voluntary policy instruments. We illustrate the technique by estimating the effectiveness of voluntary cooperation agreements between the Flemish environmental administration and individual municipalities. We focus on agreements which aim at curbing residential solid waste. Using a dataset covering all 308 Flemish municipalities for the period 2000 - 2005, our results indicate that municipalities subscribing to the agreement reduced their waste level by less than what could be expected on the basis of their own performance prior to subscription and the performance of the non-subscribers. This result might be explained by rising marginal cost of extra residential solid waste reduction policies. In addition, there are indications that subscribing municipalities refrain from additional reduction efforts once the target waste level of the program is achieved.Residential solid waste, difference-in-differences, voluntary agreements, municipalities, endogeneity bias.

Life cycle assessment and valuation of the packaging waste recycling system in Belgium

This study analyses the packaging waste management system in Belgium. Waste management operations involve a significant number of processes associated with energy consumption and emission of pollutants in air and water. To assess the impact on the environment of the several waste management operations, a life cycle assessment was developed. The operations of selective and refuse collection, sorting, recycling and incineration of packaging waste were considered. A comparison between two scenarios was developed. The first scenario comprised the packaging waste management system in operation in 2010. This system comprises the waste management operations envisaging the recycling of the packaging materials. The second scenario was developed based on the hypothesis that there was no recycling system and all packaging waste would be collected in the refuse collection system. An environmental valuation was performed to convert the environmental results into a common unit (EUR). To accomplish this valuation, three methods were used: Ecocost, Ecovalue and Stepwise. These methods were developed in Europe and follow different methodologies. The environmental results were compared using the three methods and they were consistent with the conclusion that the recycling scenario (i.e. the actual situation in 2010) is more environmentally sound

Auxin-dependent cell cycle reactivation through transcriptional regulation of Arabidopsis E2Fa by lateral organ boundary proteins

Multicellular organisms depend on cell production, cell fate specification, and correct patterning to shape their adult body. In plants, auxin plays a prominent role in the timely coordination of these different cellular processes. A well-studied example is lateral root initiation, in which auxin triggers founder cell specification and cell cycle activation of xylem pole-positioned pericycle cells. Here, we report that the E2Fa transcription factor of Arabidopsis thaliana is an essential component that regulates the asymmetric cell division marking lateral root initiation. Moreover, we demonstrate that E2Fa expression is regulated by the LATERAL ORGAN BOUNDARY DOMAIN18/LATERAL ORGAN BOUNDARY DOMAIN33 (LBD18/LBD33) dimer that is, in turn, regulated by the auxin signaling pathway. LBD18/LBD33 mediates lateral root organogenesis through E2Fa transcriptional activation, whereas E2Fa expression under control of the LBD18 promoter eliminates the need for LBD18. Besides lateral root initiation, vascular patterning is disrupted in E2Fa knockout plants, similarly as it is affected in auxin signaling and lbd mutants, indicating that the transcriptional induction of E2Fa through LBDs represents a general mechanism for auxin-dependent cell cycle activation. Our data illustrate how a conserved mechanism driving cell cycle entry has been adapted evolutionarily to connect auxin signaling with control of processes determining plant architecture

Economic viability of packaging waste recycling systems: a comparison between Belgium and Portugal

The Packaging and Packaging Waste Directive has had an undeniable impact on waste management throughout the European Union. Whereas recycling and recovery targets are the same, member states still enjoy a considerable degree of freedom with respect to the practical organization and management strategies adopted. Nevertheless, in all cases, the industry (which brings packaging material onto the market) should be responsible for the costs associated with packaging waste recycling/recovery (following the extended producer responsibility principle). The current paper compares and contrasts the institutional frameworks and financial costs and benefits of waste management operators for Belgium and Portugal. The unit costs of selective collection and sorting of packaging waste are provided for both countries. In Belgium, the costs of recycling seem to be fully supported by the industry (through Fost Plus, the national Green Dot agency). In Portugal the fairness of the recycling system depends on the perspective adopted (economic or strictly financial). Adopting a strictly financial perspective, it seems that Sociedade Ponto Verde (SPV, the Portuguese Green Dot agency) should increase the transfers to local authorities. However, the conclusions differ for this country if the avoided costs with refuse collection and other treatment are taken into account

Coulomb blockade and quantum tunnelling in the low-conductivity phase of granular metals

We study the effects of Coulomb interaction and inter-grain quantum tunnelling in an array of metallic grains using the phase-functional approach for temperatures $T$ well below the charging energy $E_{c}$ of individual grains yet large compared to the level spacing in the grains. When the inter-grain tunnelling conductance $g\gg1$, the conductivity $\sigma$ in $d$ dimensions decreases logarithmically with temperature ($\sigma/\sigma_{0}\sim1-\frac{1}{2\pi gd}\ln(gE_{c}/T)$), while for $g\to0$, the conductivity shows simple activated behaviour ($\sigma \sim \exp(-E_c/T)$). We show, for bare tunnelling conductance $g \gtrsim 1$, that the parameter $\gamma \equiv g(1-2/(g\pi)\ln(gE_{c}/T))$ determines the competition between charging and tunnelling effects. At low enough temperatures in the regime $1\gtrsim \gamma \gg 1/\sqrt{\beta E_{c}}$, a charge is shared among a finite number $N=\sqrt{(E_{c}/T)/\ln(\pi/2\gamma z)}$ of grains, and we find a soft activation behaviour of the conductivity, $\sigma\sim z^{-1}\exp(-2\sqrt{(E_{c}/T)\ln(\pi/2\gamma z)})$, where $z$ is the effective coordination number of a grain.Comment: 11 pages REVTeX, 3 Figures. Appendix added, replaced with published versio

ASAS-SN follow-up of IceCube high-energy neutrino alerts

We report on the search for optical counterparts to IceCube neutrino alerts released between April 2016 and August 2021 with the All-Sky Automated Survey for SuperNovae (ASAS-SN). Despite the discovery of a diffuse astrophysical high-energy neutrino flux in 2013, the source of those neutrinos remains largely unknown. Since 2016, IceCube has published likely-astrophysical neutrinos as public realtime alerts. Through a combination of normal survey and triggered target-of-opportunity observations, ASAS-SN obtained images within 1 hour of the neutrino detection for 20% (11) of all observable IceCube alerts and within one day for another 57% (32). For all observable alerts, we obtained images within at least two weeks from the neutrino alert. ASAS-SN provides the only optical follow-up for about 17% of IceCube's neutrino alerts. We recover the two previously claimed counterparts to neutrino alerts, the flaring-blazar TXS 0506+056 and the tidal disruption event AT2019dsg. We investigate the light curves of previously-detected transients in the alert footprints, but do not identify any further candidate neutrino sources. We also analysed the optical light curves of Fermi 4FGL sources coincident with high-energy neutrino alerts, but do not identify any contemporaneous flaring activity. Finally, we derive constraints on the luminosity functions of neutrino sources for a range of assumed evolution models

Isolation of circulating tumor cells from glioblastoma patients by direct immunomagnetic targeting

Glioblastoma (GBM) is the most common form of primary brain cancer in adults and tissue biopsies for diagnostic purposes are often inaccessible. The postulated idea that brain cancer cells cannot pass the blood-brain barrier to form circulating tumor cells (CTCs) has recently been overthrown and CTCs have been detected in the blood of GBM patients albeit in low numbers. Given the potential of CTCs to be analyzed for GBM biomarkers that may guide therapy decisions it is important to define methods to better isolate these cells. Here, we determined markers for immunomagnetic targeting and isolation of GBM-CTCs and confirmed their utility for CTC isolation from GBM patient blood samples. Further, we identified a new marker to distinguish isolated GBM-CTCs from residual lymphocytes

Isolation of circulating tumor cells from glioblastoma patients by direct immunomagnetic targeting

Glioblastoma (GBM) is the most common form of primary brain cancer in adults and tissue biopsies for diagnostic purposes are often inaccessible. The postulated idea that brain cancer cells cannot pass the blood-brain barrier to form circulating tumor cells (CTCs) has recently been overthrown and CTCs have been detected in the blood of GBM patients albeit in low numbers. Given the potential of CTCs to be analyzed for GBM biomarkers that may guide therapy decisions it is important to define methods to better isolate these cells. Here, we determined markers for immunomagnetic targeting and isolation of GBM-CTCs and confirmed their utility for CTC isolation from GBM patient blood samples. Further, we identified a new marker to distinguish isolated GBM-CTCs from residual lymphocytes

Aberrant epithelial GREM1 expression initiates colonic tumorigenesis from cells outside the stem cell niche

Hereditary mixed polyposis syndrome (HMPS) is characterized by the development of mixed-morphology colorectal tumors and is caused by a 40-kb genetic duplication that results in aberrant epithelial expression of the gene encoding mesenchymal bone morphogenetic protein antagonist, GREM1. Here we use HMPS tissue and a mouse model of the disease to show that epithelial GREM1 disrupts homeostatic intestinal morphogen gradients, altering cell fate that is normally determined by position along the vertical epithelial axis. This promotes the persistence and/or reacquisition of stem cell properties in Lgr5-negative progenitor cells that have exited the stem cell niche. These cells form ectopic crypts, proliferate, accumulate somatic mutations and can initiate intestinal neoplasia, indicating that the crypt base stem cell is not the sole cell of origin of colorectal cancer. Furthermore, we show that epithelial expression of GREM1 also occurs in traditional serrated adenomas, sporadic premalignant lesions with a hitherto unknown pathogenesis, and these lesions can be considered the sporadic equivalents of HMPS polyps