Doppler measurement integration for kinematic real-time GPS positioning

The present paper discusses the advantages of the use of Doppler shift measurements in a Kalman filter estimator in order to improve the kinematic stand-alone global positioning system positioning performance. Tests conducted in an urban environment using a single-frequency receiver demonstrate the real advantages of the proposed real- time computation technique

Size-dependent resistivity in a micro-processed YBa2Cu3O7-δ superconducting whisker

We report the results of a detailed geometrical and electrical study which has been performed on a YBa2Cu3O7-δ superconducting whisker. This sample has undergone three subsequent steps of micro-machining by means of a focused ion beam (FIB) instrument, in order to progressively decrease its cross-sectional area from ~77 to ~4 µm2, over a length of about 150 µm. A simple analytical model based on the exact shape both of the electrical contacts and of the micro-machined material has been proposed for the voltage drop; besides, an accurate geometrical characterization of all of the sample details has been performed by means of SEM microscopy. This enabled us to extract accurate electrical resistivity curves from the resistance versus temperature characteristics for each of the fabrication steps of the whisker, showing an increase of the sheet resistivity with decreasing cross-sectional area. Among the possible physical reasons for such behaviour, inelastic electron scattering at the sample surfaces has been ruled out because of the very short mean free path of carriers in YBCO. On the other hand, oxygen out-diffusion and Ga ion implantation due to the FIB processing are most likely to be responsible for the observed resistivity trend

Clinical associations of serum antiendothelial cell antibodies in patients with sudden sensorineural hearing loss

Objectives/Hypothesis: The role of antiendothelial cell antibodies in systemic vasculitis has been reported. The. aim of the study was to define the clinical associations of serum antiendothelial cell antibodies in patients with sudden sensorineural hearing loss. Study Design: A prospective study in patients with sudden sensorineural hearing loss. Methods: Serum samples were taken from 59 consecutive patients with sudden sensorineural hearing loss at time of presentation and from 28 normal control subjects. Indirect immunofluorescence assay was used to detect antiendothelial cell antibodies. Results: The prevalence of antiendothelial cell antibody detection was 54% (32 of 59 patients), with a statistically significant difference between patients and control subjects (P = .0064). Antiendothelial cell antibody positivity was significantly associated with absent recovery of hearing loss (P = .0020). Conclusions: The cytotoxicity to endothelial cells of the inner ear by antiendothelial cell antibody-positive sera might play a role in causing the stria vascularis damage in immune-mediated sudden sensorineural deafness. The appearance of antiendothelial cell antibody is related to the poor outcome of hearing loss, and its detection could be helpful in the selection of particular patients with sensorineural hearing loss for specific immunosuppressive treatments

Decline in macrolide resistance rates among Streptococcus pyogenes causing pharyngitis in children isolated in Italy

Macrolides are often used to treat group A streptococcus (GAS) infections, but their resistance rates reached high proportions worldwide. The aim of the present study was to give an update on the characteristics and contemporary prevalence of macrolide-resistant pharyngeal GAS in Central Italy. A total of 592 isolates causing pharyngitis in children were collected in the period 2012-2013. Clonality was assessed by emm typing and pulsed-field gel electrophoresis (PFGE) for all macrolide-resistant strains and for selected susceptible isolates. Genetic determinants of resistance were screened by polymerase chain reaction (PCR). Forty-four GAS were erythromycin-resistant (7.4 %). Among them, 52.3 % and 50 % were clindamycin- and tetracycline-resistant, respectively. erm(B)-positive isolates (52.3 %) expressed the constitutive cMLSB phenotype. mef(A) and its associated M phenotype were recorded in 40.9 % of the cases. The remaining erm(A)-positive isolates expressed the iMLSB phenotype. Seventeen tetracycline-resistant isolates carried tet(M) and five isolates carried tet(O). Twenty-five emm types were found among all strains, with the predominance of emm types 12, 89, 1, and 4. Eleven emm types and 12 PFGE clusters characterized macrolide-resistant strains, with almost two-thirds belonging to emm12, emm4, and emm11. Macrolide-susceptible and -resistant emm types 12, 89, 11, and 4 shared related PFGE profiles. There was a dramatic decline in macrolide resistance in Central Italy among pharyngeal GAS isolates in 2012-2013 when compared to previous studies from the same region (p < 0.05), although macrolide consumption remained stable over the past 15 years. We observed a decrease in the proportion of macrolide-resistant strains within emm types commonly associated with macrolide resistance in the past, namely emm12, 1, and 89

Different healing characteristics of thiol-bearing molecules on CVD-grown MoS₂

Vacancies in atomically thin molybdenum disulphide play an essential role in controlling its optical and electronic properties, which are crucial for applications in sensorics, catalysis or electronics. For this reason, defect engineering employing thiol-terminated molecules is used to heal and/or functionalise defective nanosheets. In this work, chemical vapour deposition-grown MoS2 with different defect densities was functionalised with three molecules: 4-aminothiophenol (ATP), biphenyl-4-thiol (BPT) and 4-nitrothiophenol (NTP). The molecules’ efficacy in functionalising MoS2 was probed by x-ray photoelectron, Raman and photoluminescence (PL) spectroscopy. The results show that exposing a defective single layer of MoS2 to either ATP, BPT or NTP molecules heals the defects, however the chemical structure of these molecules affects the optical response and only for BPT the PL intensity increases.</p

The RNA-binding protein MEX3A is a prognostic factor and regulator of resistance to gemcitabine in pancreatic ductal adenocarcinoma

Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive cancer. Most patients present with advanced disease at diagnosis, which only permits palliative chemotherapeutic treatments. RNA dysregulation is a hallmark of most human cancers, including PDAC. To test the impact of RNA processing dysregulation on PDAC pathology, we performed a bioinformatics analysis to identify RNA-binding proteins (RBPs) associated with prognosis. Among the 12 RBPs associated with progression-free survival, we focused on MEX3A because it was recently shown to mark an intestinal stem cell population that is refractory to chemotherapeutic treatments, a typical feature of PDAC. Increased expression of MEX3A was correlated with higher disease stage in PDAC patients and with tumor development in a mouse model of PDAC. Depletion of MEX3A in PDAC cells enhanced sensitivity to chemotherapeutic treatment with gemcitabine, whereas its expression was increased in PDAC cells selected upon chronic exposure to the drug. RNA-sequencing analyses highlighted hundreds of genes whose expression is sensitive to MEX3A expression, with significant enrichment in cell cycle genes. MEX3A binds to its target mRNAs, like cyclin-dependent kinase 6 (CDK6), and promotes their stability. Accordingly, knockdown of MEX3A caused a significant reduction in PDAC cell proliferation and in progression to the S phase of the cell cycle. These findings uncover a novel role for MEX3A in the acquisition and maintenance of chemoresistance by PDAC cells, suggesting that it may represent a novel therapeutic target for PDAC

The use of high aspect ratio photoresist (SU-8) for super-hydrophobic pattern prototyping

In this work we present a reliable technique for the production of large areas of high aspect-ratio patterns and describe their use as model super-hydrophobic systems. The high thickness and straight sidewalls possible with SU-8 were used to generate dense patterns of small pillars. These photoresist patterns could be used directly, without the need for micromoulding. A method is given allowing resist thickness to be varied over a wide range and a bottom antireflective layer was used to simplify patterning on reflective substrates. This patterning technique allows rapid testing of wetting theories, as pattern size and depth can be varied simply and samples can be produced in sufficient numbers for laboratory use. We show how the static contact angle of water varies with pattern height for one sample-pattern and how static and dynamic contact angles vary with dimension using high aspect-ratio patterns

Solar neutrino detection in a large volume double-phase liquid argon experiment

Precision measurements of solar neutrinos emitted by specific nuclear reaction chains in the Sun are of great interest for developing an improved understanding of star formation and evolution. Given the expected neutrino fluxes and known detection reactions, such measurements require detectors capable of collecting neutrino-electron scattering data in exposures on the order of 1 ktonne yr, with good energy resolution and extremely low background. Two-phase liquid argon time projection chambers (LAr TPCs) are under development for direct Dark Matter WIMP searches, which possess very large sensitive mass, high scintillation light yield, good energy resolution, and good spatial resolution in all three cartesian directions. While enabling Dark Matter searches with sensitivity extending to the "neutrino floor" (given by the rate of nuclear recoil events from solar neutrino coherent scattering), such detectors could also enable precision measurements of solar neutrino fluxes using the neutrino-electron elastic scattering events. Modeling results are presented for the cosmogenic and radiogenic backgrounds affecting solar neutrino detection in a 300 tonne (100 tonne fiducial) LAr TPC operating at LNGS depth (3,800 meters of water equivalent). The results show that such a detector could measure the CNO neutrino rate with ~15% precision, and significantly improve the precision of the 7Be and pep neutrino rates compared to the currently available results from the Borexino organic liquid scintillator detector.Comment: 21 pages, 7 figures, 6 table

Seismic analysis and retrofitting of an existing R.C. highway bridge: investigation through pseudo-dynamic

The “Retro” TA project funded by the European commission within the Series-project aims at studying numerically and experimentally the seismic behaviour of an old existing reinforced concrete bridge with portal frame piers and the effectiveness of different isolation systems. In particular, an experimental test campaign will be performed at ELSA Laboratory of JRC (Ispra, Italy). Two piers (scale 1:2.5) will be built and tested using the PsD technique with sub-structuring; the modelling of he entire viaduct is considered along with the non-linear behaviour of each pier, due to bending, shear on the transverse beams and strain penetration effect at the column bases. The comprehensive numerical investigations have shown the high vulnerability of the sample bridge. Consequently two isolation systems (yielding-based and friction-based bearings) have been currently designed and characterized. Because the test will start after the summer 2012, in this paper the relevant issues will be here addressed and discussed.JRC.G.5-European laboratory for structural assessmen