Monitoring of Air Pollution by Moss Bags around an Oil Refinery: A Critical Evaluation over 16 Years

The present study analyzes the results of a biomonitoring campaign, carried out by means of Hypnum cupressiforme Hedw. moss bags around an oil refinery, located in the southwestern part of Sardinia island (Italy). This work focuses mainly on the effects of rainfall and distance from the source of contamination on the content of 14 trace elements measured over 16 years. In addition, to point out any increasing or decreasing trends, as well as any peak in presence of airborne pollutants in the area, annual elements' concentration values are plotted and discussed. Coefficients of variation were also calculated on accumulation values in order to evaluate stability of measurements across the years and to evaluate if similar exposure conditions, i.e., humidity and distance from contamination source, resulted in more uniform accumulation values. In conclusion, (i) the vicinity of the source of contamination as well as rainfall influenced element content in the biomonitor in the case study differently, depending on the considered element and on the exposure condition, (ii) H. cupressiforme moss bags provided relatively stable measurements during the 16-year time frame (observed variations in elements content can be attributed to environmental inputs in the area), (iii) similar conditions of exposure determined less variable accumulation values

Epipactis tremolsii seed diversity in two close but extremely different populations: Just a case of intraspecific variability?

Analysis of the seed morphology is a widely used approach in ecological and taxonomic studies. In this context, intraspecific variability with respect to seed morphology (size, weight, and density) was assessed in two close Epipactis tremolsii Pau. populations sharing the same ecological conditions, except for the soil pollution distinguishing one of them. Larger and heavier seeds were found in plants growing on the heavy metal polluted site, while no differences in seed density were detected between seeds produced by plants growing on the contaminated and the control site. Moreover, seed coats and embryos varying together in their dimensions were described in the control population, while coats varying in their size independently from embryos were described in plants growing on the polluted site. Seeds from the two studied populations significantly differed in several parameters suggesting that intraspecific seed variability occurred in the case study

Resolution of a Severe Grade of Obstructive Sleep Apnea Syndrome with Mandibular Advancement Device: A Case Report

Obstructive sleep apnea syndrome (OSAS) is a disorder of the sleep with episodes of the obstruction at the upper airway that can be recurrent, partial or complete. The continuous positive airway pressure (CPAP) is the gold treatment for OSAS, but many patients do not tolerate this kind of treatment, and for this reason mandibular advancement device are used. A 55-year-old Caucasian man affected by severe OSAS [apnea-hypopnea index (AHI): 51, lowest saturation value (NADIR): 77%] came at Padua University Hospital Dental Clinic after ENT evaluation to consider an alternative treatment to CPAP. After a surgery treatment and the use of oral appliance (Somnodent, Somnomed Limited) associated with vertical elastics AHI improve to 2.4 and NADIR to 88%

Heavy metal tolerance strategies in metallicolous and non-metallicolous populations of mosses: Insights of γ+β-tocopherol regulatory role

As bryophytes present the capacity to colonize a wide range of habitats, including extreme environments (e.g., mine areas), we first checked the global response of bryophytes to heavy metals (HM) by a systematic review. This analysis found i) the lack of studies in a polluted environment and ii) resulted in a global trend of photochemical efficiency decrease when bryophytes faced heavy metals. Secondly, we characterized the photoprotective responses of four metallicolous populations of mosses (Lewinskya rupestris, Polytrichum commune, Ptychostomum compactum and Rhynchostegium confertum) naturally growing in an abandoned mine area by an experimental field approach. As a result, we found markedly species-specific tolerance patterns: i) less tolerant species, presenting high pollutants content accompanied by marked dissipative and antioxidative strategies. This strategy was depicted by the species R. confertum that result to be a good sentinel species due to the high pollutant content and high physiological sensitivity.; ii) intermediate tolerant species presenting a medium content of pollutants and less marked photoprotective mechanisms, and iii) most tolerant species minimizing pollutants content and consequently presenting no changes in their physiological performance. This evidence support that species’ bryological attributes have a key role in determining species’ tolerance towards environmental heavy metals, and should be taken into consideration in future studies. The findings of this study also pointed out that the higher levels of γ + β-tocopherol in metallicolous populations could play a regulatory role in metal stress tolerance in mosses and it could be a suitable functional responsive trait for environmental response prediction to heavy metals in polluted environments

Neoplasia at the site of the colostomy of patient with chagasic megacolon: case report

A neoplasia no sítio da colostomia associada ao megacólon chagásico é uma entidade rara. Os autores relatam caso de um paciente com lesão avançada, o qual foi submetido a tratamento cirúrgico e discutem aspectos relacionados a esta afecção.Neoplasia at the site of the colostomy associated with chagasic megacolon is a rare clinical event. Here, the authors report the case of a patient with advanced lesion, who had to undergo surgical treatment, and discuss aspects related to this disease

Volatile Organic Compounds (VOCs) Diversity in the Orchid Himantoglossum robertianum (Loisel.) P. Delforge from Sardinia (Italy)

Volatile Organic Compounds (VOCs) are produced by plants to address a variety of physiological and ecological tasks (among others, stress resistance, and pollinator attraction). Genetics is a key factor in determining plants’ VOCs content and emission, nevertheless, environment strongly influences VOCs profiles in plants. Orchids are a widespread group of plants that colonize diverse environments and rely on complex and refined pollination mechanisms to reproduce. Orchids VOCs are rarely studied and discussed in relation to growing conditions. In the present study, we compare the volatile profiles of inflorescences of Himantoglossum robertianum (Loisel.) P. Delforge sampled in six ecologically diverse populations on Sardinia Island (Italy). The essential oils obtained by steam distillation were characterized by GC‐FID and GC‐MS analysis. A total of 79 compounds were detected, belonging to the chemical classes of saturated hydrocarbons, esters, alcohols, ketones, unsaturated hydrocarbons, sesquiterpenes, oxygenated terpenes, terpenes, acids, and aldehydes. Multivariate statistics separated H. robertianum populations based on their chemical profiles. Differences were positively linked to the distance separating populations and reflected climatological features of the sampling sites. Interestingly, our results differed from those available in the literature, pointing out the high variability of VOCs profiles in this food‐deceptive orchid

The Oral Dipeptidyl Peptidase-4 Inhibitor Sitagliptin Increases Circulating Endothelial Progenitor Cells in Patients With Type 2 Diabetes: Possible role of stromal-derived factor-1α

OBJECTIVE: Vasculoprotective endothelial progenitor cells (EPCs) are regulated by stromal-derived factor-1alpha (SDF-1alpha) and are reduced in type 2 diabetes. Because SDF-1alpha is a substrate of dipeptidyl-peptidase-4 (DPP-4), we investigated whether the DPP-4 inhibitor sitagliptin modulates EPC levels in type 2 diabetic patients. RESEARCH DESIGN AND METHODS: This was a controlled, nonrandomized clinical trial comparing 4-week sitagliptin (n = 16) versus no additional treatment (n = 16) in addition to metformin and/or secretagogues in type 2 diabetic patients. We determined circulating EPC levels and plasma concentrations of SDF-1alpha, monocyte chemoattractant protein-1 (MCP-1), vascular endothelial growth factor (VEGF), and nitrites/nitrates. RESULTS: There was no difference in clinical baseline data between the sitagliptin and control arms. After 4 weeks, as compared with control subjects, patients receiving sitagliptin showed a significant increase in EPCs and SDF-1alpha and a decrease in MCP-1. CONCLUSIONS: Sitagliptin increases circulating EPCs in type 2 diabetic patients with concomitant upregulation of SDF-1alpha. This ancillary effect of DPP-4 inhibition might have potential favorable cardiovascular implications

Time Course and Mechanisms of Circulating Progenitor Cell Reduction in the Natural History of Type 2 Diabetes

OBJECTIVE: Reduction of bone marrow-derived circulating progenitor cells has been proposed as a novel mechanism of cardiovascular disease in type 2 diabetes. The present study was designed to describe the extent and potential mechanisms of progenitor cell reduction during the natural history of type 2 diabetes. RESEARCH DESIGN AND METHODS: We identified 425 individuals, divided into seven categories according to carbohydrate metabolism status (normal glucose tolerance [NGT], impaired fasting glucose, impaired glucose tolerance [IGT], and newly diagnosed type 2 diabetes) and diabetes duration (0-9, 10-19, and >or=20 years). These categories were examined as ideally describing the natural history of type 2 diabetes development and progression. We measured CD34+ and CD34+KDR+ progenitor cells by flow cytometry. We also evaluated progenitor cells in 20 coupled bone marrow and peripheral blood samples and examined progenitor cell apoptosis in 34 subjects. RESULTS: In comparison to NGT, CD34+ cells were significantly reduced in IGT and had a first nadir in newly diagnosed type 2 diabetes and a second nadir after 20 years of diabetes. Statistical adjustment for possible confounders confirmed that CD34+ cell counts are deeply reduced at time of diagnosis, that they partially recover during the subsequent 0-19 years, and that they dip again after >or=20 years. A similar, but less consistent, trend was detected for CD34+KDR+ cells. Peripheral blood CD34+ cells were directly correlated with bone marrow CD34+ cells and inversely correlated with CD34+ cell apoptosis. CONCLUSIONS: Circulating progenitor cell reduction marks the clinical onset of type 2 diabetes. Both defective mobilization and increased apoptosis may account for this phenomenon. While a partial recovery occurs during subsequent years, bone marrow reserve seems exhausted in the long term

On an accidental degeneracy in the 31P{¹H} NMR chemical shifts in ruthenium diphosphine complexes

The [RuCl(bipy)(dppb)(4-pic)]PF6 complex was prepared and fully characterized. The X-ray crystal structure of this complex was determined in order to make an unambiguous distinction between the two possible positions of the 4-methylpyridine ligand (4-pic) in the compound: trans to phosphorus atom or trans to nitrogen atom. The [RuCl(bipy)(dppb)(4-pic)]PF6 complex exhibits an unusual temperature-dependent accidental degeneracy of the 31P chemical shifts in its solution NMR spectrum

Promising inhibition of diabetes-related enzymes and antioxidant properties of Ptilostemon casabonae leaves extract

Type 2 diabetes (T2D) is a progressive metabolic disorder of glucose metabolism. One of the therapeutic approaches for the treatment of T2D is reducing postprandial hyperglycaemia through inhibition of the digestive enzymes α-glucosidase and α-amylase. In this context, aimed at identifying natural products endowed with anti-T2D potential, we focused on Ptilostemon casabonae (L.) Greuter, a species belonging to Asteraceae family. Enzymatic inhibition, antioxidant activity, phenolic composition and cellular assays were performed. This study revealed that the P. casabonae hydroalcoholic extract exerts a potent inhibitory activity against α-glucosidase. This activity is supported by an antioxidant effect, preventing ROS formation in a stressed cellular system. HPLC-PDA-MS/MS analysis, revealed a complex polyphenolic fraction. Among the tested pure compounds, 1,5-dicaffeoylquinic acid, apigenin and rutin displayed good α-glucosidase inhibitory activity. Our study suggested new potential of P. casabonae encouraging us to further testing the possible therapeutic potential of this extract