21 research outputs found
Nitrogen metabolism in forest trees
NITROGEN METABOLISM IN FOREST TREES
Francisco Cánovas, Concepción Ávila, Fernando N de la Torre, Rafael Cañas, Belén Pascual.
Universidad de Málaga
Email:[email protected]
Forests are essential components of the ecosystems covering approximately one-third of the Earth’s land area and playing a fundamental role in the regulation of terrestrial carbon sinks. Forest trees are also of significant economic importance, as they are used for timber and paper production worldwide. A sustainable management of forest resources is needed to preserve natural forest and to meet the increasing international demands in the production of wood and the other forest-derived products. New advances and developments in biotechnology will contribute to accelerate the domestication of important traits for forest productivity. It is critical to identify the fundamental constraints on forest productivity to addressing these constraints with modern genomic tools. Nitrogen availability extremely low in forest ecosystems, and consequently, forest trees have evolved adaptive mechanism and biotic interactions to guarantee the strict economy of this essential nutrient. Nitrogen assimilation and recycling play a key role in the tree growth and biomass production and we firmly believe that knowledge on nitrogen metabolism will lead to approaches aimed at increasing forest productivity. In our laboratory, we are interested in studying nitrogen metabolism and its regulation the conifer maritime pine (Pinus pinaster Aiton), a forest tree species of great economic and ecological importance in the Mediterranean area and relevant model for conifer genomic research in Europe.
Current research efforts are focused on improving the understanding of the response of conifer trees to ammonium availability and the transcriptional control of ammonium assimilation into amino acids. An overview and update of our research programme will be presented and discussed. Research supported by Spanish Ministry of Economy and Competitiveness and Junta de Andalucía (Grants BIO2012-33797, PLE2009-016 and research group BIO-114).Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech
Impactos ambientais do manejo agroecológico da caatinga no Rio Grande do Norte.
O objetivo deste trabalho foi avaliar os impactos ambientais do manejo agroecológico da caatinga, em unidades de produção familiar no Rio Grande do Norte, pelo método Ambitec de produção animal - dimensão ambiental, desenvolvido pela Embrapa Meio Ambiente. Foram avaliadas sete unidades de produção familiar, em quatro projetos de assentamentos de reforma agrária do Município de Apodi, RN. Os dados para o levantamento foram obtidos por meio de questionários aplicados aos representantes das unidades produtivas familiares, que atribuíram, a cada variável estudada, um valor que representou a alteração proporcionada pela implementação da tecnologia. Após a inserção dos coeficientes de alteração de cada variável dos indicadores por unidade de produção, o coeficiente de impacto foi automaticamente calculado por meio da planilha Ambitec. O manejo agroecológico da caatinga resultou num impacto ambiental positivo, e suas maiores contribuições foram relacionadas aos efeitos positivos dos seguintes indicadores: capacidade produtiva do solo, uso de insumos materiais, qualidade do produto e diminuição da emissão de poluentes à atmosfera. Dois indicadores geraram efeitos negativos: o uso de energia e o uso de recursos naturais. Pela superioridade dos benefícios gerados, o manejo agroecológico da caatinga é uma inovação tecnológica geradora de impactos ambientais positivos
Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries
Background
Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres.
Methods
This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries.
Results
In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia.
Conclusion
This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries
Recuperação de oócitos e produção in vitro de embriões de vacas estimuladas com fsh ou ecg
It was evaluated the oocyte and embryo in
vitro production of 42 Nellore mixed breed cows
(Bos taurus indicus) come from de same genetic
group, aging from 4 to 9 years, with mean body
weight of 420 kg, stimulated with FSH and eCG.
The animals were distributed into three groups:
group 1, control (n= 14), only with follicular aspi-
ration (OPU); group 2, treated with 1400 UI of eCG
in a single dose plus OPU (n= 14); and group 3,
treated with 120 UI of FSH administered four times
plus OPU with 12 h of interval (n= 14). All groups
received auricular implant with 3 mg of Norgestomet
on the first day (D0) associated with 2 mg of
estradiol benzoate intramuscularly. At the seventh
day (D7) the implants were removed and the
ovarian aspirations were realized on the cows of
group 1(OPU). Group 2 had the eCG applied at the
fifth day and the OPU was performed at D7. The
animals from group 3 were treated with FSH at fifth
and sixth day and the aspiration occurred at D7
(OPU). The follicular aspiration procedure was
done via transvaginal guided with ultrasound. It
was performed 42 aspirations obtaining 627
oocytes, where 502 were viable and 125 non-
viable. Then, it was realized the in vitro maturation
and fecundation. The cleavage, blastocyst and
eclosion rate were evaluated. There were no
significant difference (p>0.05) at these parameters
between the groups. In conclusion, the gona-
dotrophic stimulation with FSH and eCG, at the
doses used in this study, was insufficient to
increase the quality of the viable oocytes submitted
to in vitro fertilization to produce embryos.Avaliou-se a recuperação de oócitos e
produção de embriões in vitro de 42 vacas, mestiça
Nelore proveniente de grupo genético homogêneo,
com idade de 4 a 9 anos, com peso médio de 420
kg, estimuladas com FSH ou eCG. Estas foram
distribuídas em três grupos: grupo 1, controle
(n=14), apenas OPU, grupo 2, tratadas com 1400
UI de eCG, em dose única seguida de aspiração
folicular (OPU) (n= 14); e, grupo 3, tratadas com
120 UI de FSH, administrados com intervalo de 12
horas em quatro doses seguida de OPU (n= 14).
Todos os grupos receberam implante auricular
contendo 3 mg de Norgestomet no primeiro dia (D0)
associado a administração de 2 mg de benzoato
de estradiol via intramuscular. No sétimo dia (D7)
foram retirados todos os implantes e na sequência
foram realizadas as aspirações ovarianas das
vacas do grupo 1. O grupo 2 recebeu a aplicação
de eCG no quinto dia e a OPU foi realizada no D7.
Já o grupo 3 recebeu o tratamento com FSH no
quinto e sexto dia, e foram aspiradas em D7. O
procedimento de aspiração folicular foi realizado
via transvaginal guiada por ultrassom. Foram rea-
lizadas 42 aspirações com obtenção de 627 oócitos,
sendo 502 viáveis e 125 inviáveis. Realizou-se a
maturação e fecundação in vitro. Avaliaram-se as
taxas de clivagem, blastocisto e eclosão. Não
houve diferenças significativas (p>0,05) nos
parâmetros avaliados entre os grupos. Conclui-se
que o estímulo ovariano com FSH ou com eCG nas
doses utilizadas, foram insuficientes para incre-
mentar o número e a qualidade de oócitos viáveis
submetidos a fertilização in vitro para produção
de embriões